Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F78 I0 R1	626	241.3	10389380	77.7%	8072548	149.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	632,406	T→A	11.9%	K223N (AAA→AAT)	ybdO ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	632,406	0	T	A	11.9%	719.3 / 73.5	227	K223N (AAA→AAT)	ybdO	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (102/98); new base A (12/15); total (114/113)
Fisher's exact test for biased strand distribution p-value = 5.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 232 total aligned reads displayed.

ATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGT  >  CP009273/632257‑632555

 ttGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGAttt                                                                                                                                                      <  1:4917432/150‑1 (MQ=255)

   gAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTg                                                                                                                                                    <  1:3246214/150‑1 (MQ=255)

      ggTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGa                                                                                                                                                 <  2:352877/150‑1 (MQ=255)

      ggTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGa                                                                                                                                                 >  1:2177184/1‑150 (MQ=255)

       gTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGAt                                                                                                                                                <  2:3766150/150‑1 (MQ=255)

       gTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGAt                                                                                                                                                >  2:3537364/1‑150 (MQ=255)

          aCGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCa                                                                                                                                             <  2:717743/150‑1 (MQ=255)

           cGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCAt                                                                                                                                            <  2:4168134/150‑1 (MQ=255)

           cGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCAt                                                                                                                                            >  1:2489416/1‑150 (MQ=255)

            ggATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATa                                                                                                                                           >  1:451290/1‑150 (MQ=255)

             gATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATaa                                                                                                                                          >  1:33814/1‑150 (MQ=255)

             gATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATaa                                                                                                                                          <  1:504556/150‑1 (MQ=255)

              aTGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATaaa                                                                                                                                         <  1:3859279/150‑1 (MQ=255)

               tGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAt                                                                                                                                        >  2:1402972/1‑150 (MQ=255)

                gTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAtc                                                                                                                                       >  1:1389742/1‑150 (MQ=255)

                gTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAAtc                                                                                                                                       <  1:3872616/150‑1 (MQ=255)

                 ttCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAtct                                                                                                                                      <  2:4904006/150‑1 (MQ=255)

                 ttCGAGTTTTATCTCTTTGAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAAtct                                                                                                                                      <  2:2663340/150‑1 (MQ=255)

                  tCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATCATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAGATGAGATTTTGCGATTCATAAAtctc                                                                                                                                     <  2:3296809/150‑1 (MQ=255)

                     aGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCg                                                                                                                                  >  2:5143771/1‑150 (MQ=255)

                           aTCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTAtt                                                                                                                            >  2:4352756/1‑150 (MQ=255)

                              tctTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTcc                                                                                                                         >  1:871320/1‑150 (MQ=255)

                               ctTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCa                                                                                                                        >  1:636887/1‑150 (MQ=255)

                               ctTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCa                                                                                                                        >  1:3060720/1‑150 (MQ=255)

                                 tttAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCAtt                                                                                                                      <  2:1571150/150‑1 (MQ=255)

                                   taagttaagAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTg                                                                                                                    <  1:1773848/150‑1 (MQ=255)

                                     agttaagAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGaa                                                                                                                  <  1:3095107/150‑1 (MQ=255)

                                        taagAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGTatat                                                                                                               >  2:3151386/1‑150 (MQ=255)

                                          agAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATatca                                                                                                             <  2:1245289/150‑1 (MQ=255)

                                           gAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATatcat                                                                                                            <  1:766783/150‑1 (MQ=255)

                                               gTCACGGTATGAATTATCAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAAc                                                                                                        <  2:2845535/150‑1 (MQ=255)

                                               gTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAAc                                                                                                        <  1:3550142/150‑1 (MQ=255)

                                               gTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAAc                                                                                                        <  1:950813/150‑1 (MQ=255)

                                               gTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAAc                                                                                                        >  2:3345273/1‑150 (MQ=255)

                                                  aCGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAAcccc                                                                                                     <  2:830270/150‑1 (MQ=255)

                                                   cGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCct                                                                                                    <  1:2034980/150‑1 (MQ=255)

                                                     gTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCctgc                                                                                                  >  1:3276688/1‑150 (MQ=255)

                                                       aGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCctgctg                                                                                                >  2:2217211/1‑150 (MQ=255)

                                                          aaTTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGAtt                                                                                             <  2:2188898/150‑1 (MQ=255)

                                                          aaTTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGAtt                                                                                             >  2:870368/1‑150 (MQ=255)

                                                             tataAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAg                                                                                          <  2:1148702/150‑1 (MQ=255)

                                                             tataAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAg                                                                                          >  2:1946657/1‑150 (MQ=255)

                                                               taAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGat                                                                                        >  1:2937653/1‑150 (MQ=255)

                                                                  aGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATAtt                                                                                     >  2:1811777/1‑150 (MQ=255)

                                                                  aGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATAtt                                                                                     <  1:1120698/150‑1 (MQ=255)

                                                                    ttCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTaa                                                                                   >  2:4936435/1‑150 (MQ=255)

                                                                     tCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAAt                                                                                  >  1:4134887/1‑150 (MQ=255)

                                                                     tCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAAt                                                                                  <  1:283388/150‑1 (MQ=255)

                                                                     tCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATATATCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTCGATATTAAt                                                                                  >  2:2525342/1‑150 (MQ=255)

                                                                      cATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAAtt                                                                                 <  1:3706449/150‑1 (MQ=255)

                                                                      cATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAAtt                                                                                 >  1:2423090/1‑150 (MQ=255)

                                                                       aTAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTg                                                                                >  2:4775667/1‑150 (MQ=255)

                                                                        tAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTgg                                                                               <  1:4530499/150‑1 (MQ=255)

                                                                          ggggACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGt                                                                             >  1:5178696/1‑150 (MQ=255)

                                                                                  ttCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACtctt                                                                     >  1:4366785/1‑150 (MQ=255)

                                                                                   tCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTCGATATTAATTGGGTGAACtcttc                                                                    >  2:3769446/1‑150 (MQ=255)

                                                                                    cccAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACtcttct                                                                   <  1:127111/150‑1 (MQ=255)

                                                                                    cccAGTAAATCTGTAACTGCAATGATATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACtcttct                                                                   <  2:4144527/150‑1 (MQ=255)

                                                                                     ccAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTg                                                                  >  1:4244363/1‑150 (MQ=255)

                                                                                        gTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCa                                                               >  2:2660177/1‑150 (MQ=255)

                                                                                        gTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCa                                                               >  2:1506931/1‑150 (MQ=255)

                                                                                            aTCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAAt                                                           >  1:1025514/1‑150 (MQ=255)

                                                                                              cTGTAACTGCAATGCTATTAATGAGTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAAttt                                                         <  2:4240281/150‑1 (MQ=255)

                                                                                                  aaCTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTc                                                     >  1:3179191/1‑150 (MQ=255)

                                                                                                       cAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAg                                                >  1:4784299/1‑150 (MQ=255)

                                                                                                        aaTGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGt                                               >  1:260492/1‑150 (MQ=255)

                                                                                                        aaTGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGt                                               <  2:2177184/150‑1 (MQ=255)

                                                                                                        aaTGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGt                                               <  1:4196392/150‑1 (MQ=255)

                                                                                                         aTGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTg                                              >  1:4645255/1‑150 (MQ=255)

                                                                                                            cTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTa                                           <  2:4499477/150‑1 (MQ=255)

                                                                                                             tATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTAt                                          >  2:1511547/1‑150 (MQ=255)

                                                                                                             tATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTAt                                          >  1:1041045/1‑150 (MQ=255)

                                                                                                             tATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTAt                                          >  1:1041358/1‑150 (MQ=255)

                                                                                                                tAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGt                                       >  1:4327594/1‑150 (MQ=255)

                                                                                                                  aTGATTGTCAGCAAAGAGGACCCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATGTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCa                                     >  2:2060790/1‑150 (MQ=255)

                                                                                                                   tGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAg                                    <  1:2707736/150‑1 (MQ=255)

                                                                                                                   tGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAg                                    <  1:2707127/150‑1 (MQ=255)

                                                                                                                    gATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGt                                   >  1:4168861/1‑150 (MQ=255)

                                                                                                                    gATTGTCAGCAAAGAGGAACCACGAAATGAGATATTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGt                                   >  1:1573820/1‑150 (MQ=255)

                                                                                                                        gTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAAtt                               >  2:3040619/1‑150 (MQ=255)

                                                                                                                         tCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTc                              <  1:1908097/150‑1 (MQ=255)

                                                                                                                           aGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGt                            <  2:3712175/150‑1 (MQ=255)

                                                                                                                            gCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTg                           <  2:1095484/150‑1 (MQ=255)

                                                                                                                             cAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTgg                          <  2:2376916/150‑1 (MQ=255)

                                                                                                                             cAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTgg                          <  1:1402567/150‑1 (MQ=255)

                                                                                                                              aaaGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGa                         >  1:2778350/1‑150 (MQ=255)

                                                                                                                               aaGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGAt                        <  1:3630388/150‑1 (MQ=255)

                                                                                                                                agagGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATg                       >  2:571073/1‑150 (MQ=255)

                                                                                                                                 gagGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGc                      >  1:94736/1‑150 (MQ=255)

                                                                                                                                 gagGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGc                      >  1:448650/1‑150 (MQ=255)

                                                                                                                                  agGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGcc                     >  2:4029513/1‑150 (MQ=255)

                                                                                                                                  agGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGcc                     <  1:334209/150‑1 (MQ=255)

                                                                                                                                  agGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGcc                     <  1:36647/150‑1 (MQ=255)

                                                                                                                                      aCCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTAtt                 <  1:1307889/150‑1 (MQ=255)

                                                                                                                                      aCCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTAtt                 <  1:4645885/150‑1 (MQ=255)

                                                                                                                                        cACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATtac               >  2:3532356/1‑150 (MQ=255)

                                                                                                                                          cGATATGTGTTTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTTAAGTTCATCAACCCCTGCTGATTTAGTTATTAATTGGGTGTACTCTTCTGCCATAATTTGTTCATTAGTGCTATTGTCAGTATTTCGTGGATGCCTATtacta             <  2:4513947/150‑1 (MQ=255)

                                                                                                                                           gAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATtactac            <  2:1904562/150‑1 (MQ=255)

                                                                                                                                                gagaTTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAAtt       <  1:1170061/150‑1 (MQ=255)

                                                                                                                                                  gaTTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTag     <  1:690090/150‑1 (MQ=255)

ATTGAAGGTAACGGATGTTCTAGTTTTATCTCTTTTAAGTTAAGAAAGTCACGGTAGGAATTATAAAGTTCATAGGGGACTATTCCCAGTAAATCTGTAACTGCAATGCTATTAATGATTGTCAGCAAAGAGGAACCACGAAATGAGATTTTGCGATTCATAAATCTCTCGTCTATTTCCATTTGAATATCATCAACCCCTGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGT  >  CP009273/632257‑632555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A