Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,493,283 C→A 12.5% N378K (AAC→AAA)  ypdA → sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,493,2830CA12.5% 970.5 / 86.8 299N378K (AAC→AAA) ypdAsensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
Reads supporting (aligned to +/- strand):  ref base C (132/129);  new base A (18/20);  total (150/149)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

TTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGC  >  CP009273/2493134‑2493432
                                                                                                                                                     |                                                                                                                                                     
 tACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACg                                                                                                                                                      <  1:1918395/150‑1 (MQ=255)
 tACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAAAGCTCTGAACg                                                                                                                                                      >  2:4876499/1‑150 (MQ=255)
   cAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCt                                                                                                                                                    >  2:118698/1‑150 (MQ=255)
      gAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTAtt                                                                                                                                                 <  2:3799865/150‑1 (MQ=255)
      gAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTAtt                                                                                                                                                 >  1:2326308/1‑150 (MQ=255)
              ggtcggtcTGTCGCAAATCATCTCCACGCACCTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTc                                                                                                                                         >  2:639809/1‑150 (MQ=255)
                tcggtcTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCaa                                                                                                                                       <  1:41007/150‑1 (MQ=255)
                  ggtcTGTCGCAAATCATCTCTACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATc                                                                                                                                     <  2:3848430/150‑1 (MQ=255)
                    tcTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTCTTTCATCGTCAATCCg                                                                                                                                   >  1:5129863/1‑150 (MQ=255)
                      tGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTc                                                                                                                                 >  2:2030695/1‑150 (MQ=255)
                       gTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCt                                                                                                                                <  2:3341275/150‑1 (MQ=255)
                         cGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGa                                                                                                                              <  2:4999848/150‑1 (MQ=255)
                         cGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGa                                                                                                                              >  2:1982450/1‑150 (MQ=255)
                         cGCAAAGCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGa                                                                                                                              <  2:4999929/150‑1 (MQ=255)
                             aaTCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATcc                                                                                                                          <  1:128356/150‑1 (MQ=255)
                             aaTCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATcc                                                                                                                          <  1:1983081/150‑1 (MQ=255)
                               tcatcTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCgg                                                                                                                        <  1:4765965/150‑1 (MQ=255)
                                catcTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGa                                                                                                                       >  2:4308528/1‑150 (MQ=255)
                                  tctcCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATa                                                                                                                     <  1:1607282/150‑1 (MQ=255)
                                    tcCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATAcc                                                                                                                   >  2:4021212/1‑150 (MQ=255)
                                     ccACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCg                                                                                                                  <  1:3223910/150‑1 (MQ=255)
                                      cACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGc                                                                                                                 >  1:2343475/1‑150 (MQ=255)
                                      cACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGc                                                                                                                 >  1:120244/1‑150 (MQ=255)
                                             cTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAg                                                                                                          >  1:544722/1‑150 (MQ=255)
                                             cTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAg                                                                                                          <  1:4773072/150‑1 (MQ=255)
                                               ggAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGtt                                                                                                        >  1:2934511/1‑150 (MQ=255)
                                                gAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTg                                                                                                       >  2:4159755/1‑150 (MQ=255)
                                                 aGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGa                                                                                                      <  2:1099606/150‑1 (MQ=255)
                                                     ttCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCtt                                                                                                  <  2:2942097/150‑1 (MQ=255)
                                                     ttCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCtt                                                                                                  <  1:4237108/150‑1 (MQ=255)
                                                     ttCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCtt                                                                                                  <  2:703274/150‑1 (MQ=255)
                                                       cACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTa                                                                                                >  1:3710686/1‑150 (MQ=255)
                                                       cACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTa                                                                                                <  2:3759310/150‑1 (MQ=255)
                                                       cACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTa                                                                                                >  2:4236122/1‑150 (MQ=255)
                                                         cgcgCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCtttcag                                                                                              >  2:4964021/1‑147 (MQ=255)
                                                               gAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATCTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTcg                                                                                        <  2:1420648/150‑1 (MQ=255)
                                                                agcagcTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTcgc                                                                                       >  1:566671/1‑150 (MQ=255)
                                                                   agcTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGtt                                                                                    >  1:1395209/1‑150 (MQ=255)
                                                                     cTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTAt                                                                                  >  1:1458492/1‑150 (MQ=255)
                                                                     cTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTAt                                                                                  <  1:2492401/150‑1 (MQ=255)
                                                                      tACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATc                                                                                 <  2:1280965/150‑1 (MQ=255)
                                                                       aCGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCt                                                                                <  1:3992814/150‑1 (MQ=255)
                                                                        cGTGAAATGGCAAATAATGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATACCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGAGACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTg                                                                               <  1:2750091/150‑1 (MQ=255)
                                                                        cGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTg                                                                               <  1:4970805/150‑1 (MQ=255)
                                                                          tGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTgcg                                                                             >  1:5158174/1‑150 (MQ=255)
                                                                           gAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTgcgc                                                                            >  1:3872441/1‑150 (MQ=255)
                                                                            aaaTGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCt                                                                           <  2:3289016/150‑1 (MQ=255)
                                                                                ggCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATaa                                                                       >  1:1929738/1‑150 (MQ=255)
                                                                                    aaTAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACAtt                                                                   >  2:3534329/1‑150 (MQ=255)
                                                                                      tAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTAGAACATTGa                                                                 >  2:3635263/1‑150 (MQ=255)
                                                                                      tAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTGTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGa                                                                 <  2:3371915/150‑1 (MQ=255)
                                                                                       aaGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGaa                                                                >  1:745637/1‑150 (MQ=255)
                                                                                        aGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAAt                                                               >  2:152341/1‑150 (MQ=255)
                                                                                        aGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAAt                                                               >  2:3956433/1‑150 (MQ=255)
                                                                                           cAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTaa                                                            >  1:1008046/1‑150 (MQ=255)
                                                                                            agagCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTATTCTGTCTCTTTATCTGCGCTATAACATTGAATTaaa                                                           >  2:2735719/1‑150 (MQ=255)
                                                                                            agagCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAAAATTGAATTaaa                                                           >  2:4350778/1‑150 (MQ=255)
                                                                                              agCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAg                                                         <  1:4922212/150‑1 (MQ=255)
                                                                                              agCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAg                                                         >  1:4176059/1‑150 (MQ=255)
                                                                                               gCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGa                                                        <  2:2452727/150‑1 (MQ=255)
                                                                                                cTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGAc                                                       <  1:4073098/150‑1 (MQ=255)
                                                                                                   cgcgCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGAt                                                    <  2:2859451/150‑1 (MQ=255)
                                                                                                      gcCCTGCAAAGCAAAATTACTCCCCATTTTCTGTTTATCGCTCTGAACGCTATTTCATCGTCAATCCGTCTTAATCCTGATACCGCTCGACAGTTTATCTTTAATCTTTCGCTTTATCTGCGCTATAACTTTGAATTAATAGACGTTGAg                                                 <  2:2233889/150‑1 (MQ=255)
                                                                                                      gcCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAg                                                 <  2:2010098/150‑1 (MQ=255)
                                                                                                        ccTGCAAAGCTAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGAGCTATAACATTGAATTAAAAGACGATGAGCa                                               >  2:1325402/1‑150 (MQ=255)
                                                                                                        ccTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATCACAGTGAATTAAAAGACGATGAGCa                                               >  2:5075940/1‑150 (MQ=255)
                                                                                                          tGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAATACGATGAGCaaa                                             >  1:2579927/1‑150 (MQ=255)
                                                                                                           gcaaagcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCACAt                                            >  1:5010836/1‑150 (MQ=255)
                                                                                                             aaagcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCg                                          <  2:3179597/150‑1 (MQ=255)
                                                                                                             aaagcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCg                                          <  2:2995773/150‑1 (MQ=255)
                                                                                                               agcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGat                                        >  2:1006703/1‑150 (MQ=255)
                                                                                                               agcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGat                                        <  1:2239372/150‑1 (MQ=255)
                                                                                                               agcaaaATTAATCCCCATTTTATGATTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGat                                        <  2:4667332/150‑1 (MQ=255)
                                                                                                                 caaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGatat                                      <  2:3073426/150‑1 (MQ=255)
                                                                                                                  aaaaTTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATc                                     >  2:2796046/1‑150 (MQ=255)
                                                                                                                  aaaaTTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATc                                     >  2:3738571/1‑150 (MQ=255)
                                                                                                                  aaaaTTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATc                                     >  1:353741/1‑150 (MQ=255)
                                                                                                                   aaaTTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCa                                    >  1:3351089/1‑150 (MQ=255)
                                                                                                                      ttAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCaaaa                                 <  1:202107/150‑1 (MQ=255)
                                                                                                                      ttAATACCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCaaaa                                 <  1:3893372/150‑1 (MQ=255)
                                                                                                                       tAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCaaaaa                                >  1:2149070/1‑150 (MQ=255)
                                                                                                                             ccATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCt                          <  1:4250191/150‑1 (MQ=255)
                                                                                                                               aTTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGt                        >  1:1010031/1‑150 (MQ=255)
                                                                                                                                 tttCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTAt                      >  1:178398/1‑150 (MQ=255)
                                                                                                                                 tttCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTAt                      <  1:3057332/150‑1 (MQ=255)
                                                                                                                                  ttCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATc                     >  1:1981057/1‑150 (MQ=255)
                                                                                                                                  ttCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATc                     <  1:2808095/150‑1 (MQ=255)
                                                                                                                                    cTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCaa                   >  1:4701077/1‑150 (MQ=255)
                                                                                                                                      gTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAAt                 <  1:90826/150‑1 (MQ=255)
                                                                                                                                      gTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAAt                 >  1:447318/1‑150 (MQ=255)
                                                                                                                                           aCGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAg            <  1:4514857/150‑1 (MQ=255)
                                                                                                                                           aCGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAg            <  1:4378574/150‑1 (MQ=255)
                                                                                                                                             gCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGAc          <  2:2343475/150‑1 (MQ=255)
                                                                                                                                             gCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGAc          <  2:3975277/150‑1 (MQ=255)
                                                                                                                                               tctGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACta        <  2:4548792/150‑1 (MQ=255)
                                                                                                                                               tctGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACta        <  1:3846080/150‑1 (MQ=255)
                                                                                                                                                ctGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACtat       <  2:5141828/150‑1 (MQ=255)
                                                                                                                                                  gAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTatat     <  2:2230439/150‑1 (MQ=255)
                                                                                                                                                   aaCGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATAtt    >  2:1756431/1‑150 (MQ=255)
                                                                                                                                                    aCGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTg   >  2:2341563/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTGC  >  CP009273/2493134‑2493432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A