Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F55 I0 R1 519 344.2 11360120 95.0% 10792114 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,493,283 C→A 21.8% N378K (AAC→AAA)  ypdA → sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,493,2830CA21.8% 835.2 / 192.1 324N378K (AAC→AAA) ypdAsensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
Reads supporting (aligned to +/- strand):  ref base C (115/138);  new base A (36/35);  total (151/173)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 326 total aligned reads displayed.

TTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTG  >  CP009273/2493134‑2493431
                                                                                                                                                     |                                                                                                                                                    
ttACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAc                                                                                                                                                      >  1:389120/1‑150 (MQ=255)
ttACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAc                                                                                                                                                      <  2:3681857/150‑1 (MQ=255)
   cAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCt                                                                                                                                                   >  1:5168463/1‑150 (MQ=255)
     ggAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTAt                                                                                                                                                 <  1:4627433/150‑1 (MQ=255)
     ggAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTAt                                                                                                                                                 >  2:4907255/1‑150 (MQ=255)
       aaaTGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCTTTTTCTGTTTAACGCTCTGAACGCTAttt                                                                                                                                               <  2:1320668/150‑1 (MQ=255)
       aaaTGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTAttt                                                                                                                                               <  2:1805250/150‑1 (MQ=255)
       aaaTGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTAttt                                                                                                                                               <  2:2535211/150‑1 (MQ=255)
        aaTGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTc                                                                                                                                              >  2:3479837/1‑150 (MQ=255)
        aaTGGCGGTAGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTc                                                                                                                                              <  1:2871688/150‑1 (MQ=255)
         aTGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCa                                                                                                                                             <  2:2006556/150‑1 (MQ=255)
           ggCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATc                                                                                                                                           >  1:2529363/1‑150 (MQ=255)
            gCGGTAGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCg                                                                                                                                          <  1:5254088/150‑1 (MQ=255)
             cggtcggtCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGt                                                                                                                                         <  1:2350961/150‑1 (MQ=255)
               gtcggtcTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCa                                                                                                                                       <  1:3935181/150‑1 (MQ=255)
                tcggtcTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCaa                                                                                                                                      <  2:5601758/150‑1 (MQ=255)
                   gtcTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATcc                                                                                                                                   >  2:4309103/1‑150 (MQ=255)
                    tcTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCg                                                                                                                                  <  2:4998104/150‑1 (MQ=255)
                    tcTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCg                                                                                                                                  <  2:2207761/150‑1 (MQ=255)
                     cTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGt                                                                                                                                 >  2:4932529/1‑150 (MQ=255)
                            aaaTCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATc                                                                                                                          >  2:2465119/1‑150 (MQ=255)
                            aaaTCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATc                                                                                                                          <  1:739937/150‑1 (MQ=255)
                            aaaTCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATc                                                                                                                          <  1:1302867/150‑1 (MQ=255)
                            aaaTCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATc                                                                                                                          >  2:1518857/1‑150 (MQ=255)
                                catcTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGa                                                                                                                      >  2:5330116/1‑150 (MQ=255)
                                catcTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCAGTTTCTGGTTAACGCTCTGAACGCTAGTTCATCGTCAATCCGTCTGAATCCGGa                                                                                                                      >  1:3126532/1‑150 (MQ=255)
                                 atcTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGAt                                                                                                                     >  2:387507/1‑150 (MQ=255)
                                    tcCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATAcc                                                                                                                  <  1:2179042/150‑1 (MQ=255)
                                    tcCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATAcc                                                                                                                  <  1:4473046/150‑1 (MQ=255)
                                        cGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTc                                                                                                              <  2:3700984/150‑1 (MQ=255)
                                           aaCTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGcc                                                                                                           <  1:2430531/150‑1 (MQ=255)
                                           aaCTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGcc                                                                                                           <  1:1775717/150‑1 (MQ=255)
                                                gAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTg                                                                                                      >  1:4105462/1‑150 (MQ=255)
                                                gAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTg                                                                                                      <  2:17127/150‑1 (MQ=255)
                                                 aGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGa                                                                                                     <  1:3286900/150‑1 (MQ=255)
                                                 aGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGa                                                                                                     <  2:2290478/150‑1 (MQ=255)
                                                   gTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATc                                                                                                   >  1:4037759/1‑150 (MQ=255)
                                                    tttCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCt                                                                                                  >  2:4738567/1‑150 (MQ=255)
                                                        aCGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTaa                                                                                              <  1:4164338/150‑1 (MQ=255)
                                                         cgcgCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAAt                                                                                             >  2:3421084/1‑150 (MQ=255)
                                                          gcgcCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATc                                                                                            >  2:2957482/1‑150 (MQ=255)
                                                            gcCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTg                                                                                          <  1:5322851/150‑1 (MQ=255)
                                                            gcCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTg                                                                                          >  1:4496201/1‑150 (MQ=255)
                                                             ccGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGt                                                                                         >  1:2398693/1‑150 (MQ=255)
                                                              cGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTc                                                                                        >  1:3812854/1‑150 (MQ=255)
                                                              cGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTc                                                                                        <  2:5466380/150‑1 (MQ=255)
                                                                  cagcTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCCGTTGATCTTTAATCTGTCGCGt                                                                                    >  2:4538561/1‑150 (MQ=255)
                                                                   agcTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGtt                                                                                   >  2:1927737/1‑150 (MQ=255)
                                                                     cTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTAt                                                                                 <  1:1294182/150‑1 (MQ=255)
                                                                      tACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATc                                                                                >  2:755460/1‑150 (MQ=255)
                                                                      tACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATc                                                                                <  1:2039110/150‑1 (MQ=255)
                                                                      tACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATc                                                                                <  1:409050/150‑1 (MQ=255)
                                                                      tACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTATGAATCCGGATACCGCTCTCCAGTTGATCTTTAAACTGTCGCGTTATc                                                                                >  1:5457939/1‑150 (MQ=255)
                                                                            aaaTGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCt                                                                          <  1:3231352/150‑1 (MQ=255)
                                                                            aaaTGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCt                                                                          <  2:4789940/150‑1 (MQ=255)
                                                                             aaTGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCta                                                                         <  1:4287393/150‑1 (MQ=255)
                                                                             aaTGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCta                                                                         <  2:2267785/150‑1 (MQ=255)
                                                                             aaTGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCAGTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCtc                                                                         >  1:5617954/1‑149 (MQ=255)
                                                                              aTGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCtat                                                                        >  1:2748539/1‑150 (MQ=255)
                                                                              aTGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCtat                                                                        <  1:524574/150‑1 (MQ=255)
                                                                              aTGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCtat                                                                        >  2:1737901/1‑150 (MQ=255)
                                                                              aTGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCtat                                                                        <  2:197733/150‑1 (MQ=255)
                                                                                      tAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGa                                                                >  2:5097520/1‑150 (MQ=255)
                                                                                         ggCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAAtt                                                             >  2:5133316/1‑150 (MQ=255)
                                                                                         ggCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAAtt                                                             <  2:5249349/150‑1 (MQ=255)
                                                                                          gCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTa                                                            >  1:993069/1‑150 (MQ=255)
                                                                                            agagCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTaaa                                                          >  1:5284228/1‑150 (MQ=255)
                                                                                               gCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGa                                                       >  1:3570395/1‑150 (MQ=255)
                                                                                                  tCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGa                                                    <  2:166879/150‑1 (MQ=255)
                                                                                                   cgcgCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGAt                                                   <  2:1207021/150‑1 (MQ=255)
                                                                                                     cgcCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGa                                                 >  2:375270/1‑150 (MQ=255)
                                                                                                     cgcCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGa                                                 <  1:2469712/150‑1 (MQ=255)
                                                                                                          tGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCaaa                                            >  2:1530618/1‑150 (MQ=255)
                                                                                                             aaagcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCg                                         <  1:3085605/150‑1 (MQ=255)
                                                                                                              aagcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGa                                        >  1:1958866/1‑150 (MQ=255)
                                                                                                               agcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTAGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGat                                       <  1:3368862/150‑1 (MQ=255)
                                                                                                                gcaaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGata                                      >  1:3062904/1‑150 (MQ=255)
                                                                                                                 caaaATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGatat                                     >  2:5390745/1‑150 (MQ=255)
                                                                                                                  aaaaTTAATCCCCATTTTCTGTTTAACGCTCAGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATc                                    <  2:4059133/150‑1 (MQ=255)
                                                                                                                     aTTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCaaa                                 >  2:917975/1‑150 (MQ=255)
                                                                                                                     aTTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCaaa                                 <  1:3757241/150‑1 (MQ=255)
                                                                                                                     aTTAATCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGACGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCaaa                                 <  2:2663706/150‑1 (MQ=255)
                                                                                                                        aaTCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCaaaaaa                              >  1:262956/1‑150 (MQ=255)
                                                                                                                        aaTCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCaaaaaa                              >  2:103839/1‑150 (MQ=255)
                                                                                                                         aTCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAag                             <  1:4213099/150‑1 (MQ=255)
                                                                                                                         aTCCCCATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAag                             >  1:573137/1‑150 (MQ=255)
                                                                                                                              cATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTg                        <  2:3279932/150‑1 (MQ=255)
                                                                                                                              cATTTTCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTg                        <  1:3482131/150‑1 (MQ=255)
                                                                                                                               aTTTTCTGTTTAACGCTCTGAACGCTATATCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTga                       >  1:5197478/1‑149 (MQ=255)
                                                                                                                                ttttCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTa                      <  1:915415/150‑1 (MQ=255)
                                                                                                                                  ttCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATCTCAAAAAAGAGCTGTATc                    >  1:1594700/1‑150 (MQ=255)
                                                                                                                                   tCTGTTTAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAATGAGCTGTATCa                   >  2:4501946/1‑150 (MQ=255)
                                                                                                                                     tGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCaca                 >  2:800508/1‑148 (MQ=255)
                                                                                                                                     tGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCaaa                 <  2:2080341/150‑1 (MQ=255)
                                                                                                                                        ttAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTa              >  1:1212396/1‑150 (MQ=255)
                                                                                                                                        ttAACGCTCTGAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTa              >  1:227120/1‑150 (MQ=255)
                                                                                                                                         tAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTaa             >  1:1041044/1‑150 (MQ=255)
                                                                                                                                                ctGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACtat      <  1:4789552/150‑1 (MQ=255)
                                                                                                                                                  gAAAGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTatat    >  2:2869887/1‑150 (MQ=255)
                                                                                                                                                    aCGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTg  <  1:4953008/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCTGAACGCTATTTCATCGTCAATCCGTCTGAATCCGGATACCGCTCGCCAGTTGATCTTTAATCTGTCGCGTTATCTGCGCTATAACATTGAATTAAAAGACGATGAGCAAATCGATATCAAAAAAGAGCTGTATCAAATTAAAGACTATATTG  >  CP009273/2493134‑2493431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A