Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,674,941 G→T 26.7% I388I (ATC→ATA)  yphG ← DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,674,9410GT26.7% 620.1 / 226.7 299I388I (ATC→ATA) yphGDUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (96/123);  new base T (34/46);  total (130/169)
Fisher's exact test for biased strand distribution p-value = 8.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

TGATGTTCCTGATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGAAATACTG  >  CP009273/2674792‑2675090
                                                                                                                                                     |                                                                                                                                                     
tGATGTTCCTGATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCGGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCg                                                                                                                                                       >  1:1924967/1‑150 (MQ=255)
tGATGTTCCAGATAACTCAGGACAAGAGTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTGGCATTTACCg                                                                                                                                                       <  2:2693344/150‑1 (MQ=255)
    gTTCCTGATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGAttt                                                                                                                                                   <  1:2100155/150‑1 (MQ=255)
            tAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATc                                                                                                                                           <  2:2261441/150‑1 (MQ=255)
               cTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGcc                                                                                                                                        <  1:3507678/150‑1 (MQ=255)
                 cAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAg                                                                                                                                      <  2:2351415/150‑1 (MQ=255)
                  aGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAgg                                                                                                                                     >  1:4034461/1‑150 (MQ=255)
                      cAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGt                                                                                                                                 <  2:1253145/150‑1 (MQ=255)
                      cAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGt                                                                                                                                 <  2:3038888/150‑1 (MQ=255)
                       aaTATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTa                                                                                                                                >  2:1043920/1‑150 (MQ=255)
                        atatTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGTTACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTAt                                                                                                                               <  2:3736709/150‑1 (MQ=255)
                         tatTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATc                                                                                                                              <  1:4279572/150‑1 (MQ=255)
                          atTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATcc                                                                                                                             <  2:341183/150‑1 (MQ=255)
                           ttGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCg                                                                                                                            >  2:4477159/1‑150 (MQ=255)
                             gCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGtt                                                                                                                          <  1:2315444/150‑1 (MQ=255)
                              ccGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTc                                                                                                                         <  2:2543352/150‑1 (MQ=255)
                               cGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa                                                                                                                        >  2:602313/1‑150 (MQ=255)
                               cGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCa                                                                                                                        >  2:3435397/1‑150 (MQ=255)
                                 tCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAAc                                                                                                                      <  1:1810712/150‑1 (MQ=255)
                                 tCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAAc                                                                                                                      <  2:5237763/150‑1 (MQ=255)
                                     cATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgag                                                                                                                  >  2:3390840/1‑150 (MQ=255)
                                        cGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgagaga                                                                                                               >  1:2178819/1‑150 (MQ=255)
                                          gagaTCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg                                                                                                             <  2:2124084/150‑1 (MQ=255)
                                              gCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCAt                                                                                                         <  1:1216210/150‑1 (MQ=255)
                                                tCAATGGTCAGCCTTTCAGGAGTGAGACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATAc                                                                                                       <  2:3665361/150‑1 (MQ=255)
                                                  aaTGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAg                                                                                                     <  2:4767696/150‑1 (MQ=255)
                                                    tGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGcc                                                                                                   >  1:4605111/1‑150 (MQ=255)
                                                      gTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGcccc                                                                                                 >  1:2959323/1‑150 (MQ=255)
                                                        cAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCa                                                                                               >  1:1189843/1‑150 (MQ=255)
                                                        cAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCa                                                                                               <  2:4167868/150‑1 (MQ=255)
                                                          gCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACt                                                                                             >  1:1365800/1‑150 (MQ=255)
                                                          gCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACt                                                                                             <  2:754873/150‑1 (MQ=255)
                                                          gCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACt                                                                                             <  1:5189679/150‑1 (MQ=255)
                                                           ccTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTc                                                                                            >  2:426243/1‑150 (MQ=255)
                                                           ccTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTc                                                                                            >  2:3698279/1‑150 (MQ=255)
                                                               tCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATc                                                                                        <  1:2158051/150‑1 (MQ=255)
                                                               tCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATc                                                                                        <  1:4760938/150‑1 (MQ=255)
                                                                 aGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATccc                                                                                      >  2:516022/1‑150 (MQ=255)
                                                                   gATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATccccc                                                                                    <  1:1038006/150‑1 (MQ=255)
                                                                        aTACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTta                                                                               <  1:4503331/150‑1 (MQ=255)
                                                                          aCCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTtact                                                                             <  2:164463/150‑1 (MQ=255)
                                                                           ccGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTtacta                                                                            <  1:3839386/150‑1 (MQ=255)
                                                                             gTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACg                                                                          >  1:2769895/1‑150 (MQ=255)
                                                                             gTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACg                                                                          >  2:182558/1‑150 (MQ=255)
                                                                                cAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTg                                                                       <  2:4937671/150‑1 (MQ=255)
                                                                                 aaCACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTgg                                                                      >  1:2481062/1‑150 (MQ=255)
                                                                                   cacGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAg                                                                    <  1:828703/150‑1 (MQ=255)
                                                                                      gCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGttt                                                                 <  2:4593850/150‑1 (MQ=255)
                                                                                       cccTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGtttt                                                                <  1:2551288/150‑1 (MQ=255)
                                                                                       cccTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGtttt                                                                >  1:1400122/1‑150 (MQ=255)
                                                                                       cccTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGtttt                                                                <  1:4217077/150‑1 (MQ=255)
                                                                                        ccTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTa                                                               >  1:4525711/1‑150 (MQ=255)
                                                                                        ccTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTa                                                               >  1:3094067/1‑150 (MQ=255)
                                                                                           ggATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGGTCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCa                                                            >  1:4958383/1‑150 (MQ=255)
                                                                                           ggATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCa                                                            >  1:763359/1‑150 (MQ=255)
                                                                                              tGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCGGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCg                                                         >  2:4995522/1‑150 (MQ=255)
                                                                                                 cGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCAt                                                      <  1:5179747/150‑1 (MQ=255)
                                                                                                  ggTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATc                                                     >  1:556840/1‑150 (MQ=255)
                                                                                                   gTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATcg                                                    <  1:3391289/150‑1 (MQ=255)
                                                                                                         ggCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAg                                              <  2:2322185/150‑1 (MQ=255)
                                                                                                           cATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGc                                            <  2:4739223/150‑1 (MQ=255)
                                                                                                            aTCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCa                                           <  1:1489520/150‑1 (MQ=255)
                                                                                                             tCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCAt                                          <  2:2464992/150‑1 (MQ=255)
                                                                                                                gTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCAtttt                                       <  2:3507844/150‑1 (MQ=255)
                                                                                                                 tGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCACTCCCCCGCTTCCTACGCTGGAGATTTATCACCGCATCGCGGGAGGCATTATg                                      >  2:2826202/1‑150 (MQ=255)
                                                                                                                    cACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCAc                                   <  1:2351054/150‑1 (MQ=255)
                                                                                                                      cGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCa                                 <  2:3526267/150‑1 (MQ=255)
                                                                                                                       ggCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCAt                                <  1:4346536/150‑1 (MQ=255)
                                                                                                                       ggCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATACCCCGCTTACTAAGCTGGAGTATTATCACCCCATCGCGGGAGGCATATTGCACCAt                                >  2:1950588/1‑150 (MQ=255)
                                                                                                                       ggCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCAt                                >  1:598329/1‑150 (MQ=255)
                                                                                                                       ggCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCAt                                <  2:423764/150‑1 (MQ=255)
                                                                                                                        gCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATg                               <  1:3695641/150‑1 (MQ=255)
                                                                                                                          atcatcAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGcc                             >  1:3009936/1‑150 (MQ=255)
                                                                                                                          atcatcAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGcc                             >  1:3424195/1‑150 (MQ=255)
                                                                                                                           tcatcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGccc                            <  2:4138167/150‑1 (MQ=255)
                                                                                                                             atcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTAATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCaa                          <  1:1693332/150‑1 (MQ=255)
                                                                                                                              tcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCaaa                         >  2:1525841/1‑150 (MQ=255)
                                                                                                                                 aGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGaa                      >  1:2879358/1‑150 (MQ=255)
                                                                                                                                  gTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAAt                     <  1:4261845/150‑1 (MQ=255)
                                                                                                                                  gTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAAt                     <  1:1733365/150‑1 (MQ=255)
                                                                                                                                   tAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATg                    >  1:5040065/1‑150 (MQ=255)
                                                                                                                                   tAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATg                    >  1:4686007/1‑150 (MQ=255)
                                                                                                                                    aaCGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGa                   >  2:1121282/1‑150 (MQ=255)
                                                                                                                                    aaCGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGa                   <  2:4426457/150‑1 (MQ=255)
                                                                                                                                     aCGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGAt                  <  2:1766185/150‑1 (MQ=255)
                                                                                                                                     aCGCGGTGCATGTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGAt                  <  2:4842347/150‑1 (MQ=255)
                                                                                                                                       gcgTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATaa                <  2:4606809/150‑1 (MQ=255)
                                                                                                                                       gcgTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATaa                <  1:3186237/150‑1 (MQ=255)
                                                                                                                                       gcgTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATaa                <  1:3936780/150‑1 (MQ=255)
                                                                                                                                       gcgTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATaa                <  1:3109441/150‑1 (MQ=255)
                                                                                                                                        cgTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAg               >  2:3978182/1‑150 (MQ=255)
                                                                                                                                          ttGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGc             >  2:2444600/1‑150 (MQ=255)
                                                                                                                                           tGCATTGACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCa            <  2:3622525/150‑1 (MQ=255)
                                                                                                                                             cATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAgg          <  2:1412122/150‑1 (MQ=255)
                                                                                                                                               tttACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCTACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGaa        >  2:124776/1‑150 (MQ=255)
                                                                                                                                               tttACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGaa        <  2:3094701/150‑1 (MQ=255)
                                                                                                                                                ttACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGaaa       >  1:1613143/1‑150 (MQ=255)
                                                                                                                                                     gATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGTCATACAGCCCCCACTCAATCCCCCGCTAACTACGCTGGAGTTTTATCACAGCATCGCGGGAGGCATTTTGCACCATGCCCATAGAATGATAAGGAAGTAAATACTg  >  2:1353522/1‑150 (MQ=255)
                                                                                                                                                     gATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGAAATACTg  >  1:1868983/1‑150 (MQ=255)
                                                                                                                                                     gATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGAAATACTg  <  2:650393/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGATGTTCCTGATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGAAATACTG  >  CP009273/2674792‑2675090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A