Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,674,941 G→T 14.0% I388I (ATC→ATA)  yphG ← DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,674,9410GT14.0% 569.0 / 61.1 178I388I (ATC→ATA) yphGDUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base G (73/80);  new base T (12/13);  total (85/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

TGTTCCTGATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGAAATACTG  >  CP009273/2674795‑2675090
                                                                                                                                                  |                                                                                                                                                     
 gTTCCTGATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGAttt                                                                                                                                                   <  2:1098851/150‑1 (MQ=255)
  ttCCTGATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTc                                                                                                                                                  <  2:922309/150‑1 (MQ=255)
  ttCCTGATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTc                                                                                                                                                  <  2:4645739/150‑1 (MQ=255)
   tCCTGATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTcg                                                                                                                                                 <  2:3083947/150‑1 (MQ=255)
      tGATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCgg                                                                                                                                              <  1:2059561/150‑1 (MQ=255)
       gATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGa                                                                                                                                             >  1:43386/1‑150 (MQ=255)
       gATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGa                                                                                                                                             >  1:3090354/1‑150 (MQ=255)
        aTAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGAt                                                                                                                                            >  1:781854/1‑150 (MQ=255)
          aaCTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCg                                                                                                                                          <  2:538942/150‑1 (MQ=255)
            cTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGcc                                                                                                                                        >  2:323432/1‑150 (MQ=255)
            cTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGcc                                                                                                                                        >  2:322997/1‑150 (MQ=255)
              cAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAg                                                                                                                                      <  2:1558994/150‑1 (MQ=255)
               aGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAgg                                                                                                                                     <  2:1177468/150‑1 (MQ=255)
                 tACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCg                                                                                                                                   <  2:3854778/150‑1 (MQ=255)
                  aCAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCgg                                                                                                                                  <  1:4768252/150‑1 (MQ=255)
                   cAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGt                                                                                                                                 <  2:1828526/150‑1 (MQ=255)
                    aaTATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTa                                                                                                                                >  2:4538785/1‑150 (MQ=255)
                      tatTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATc                                                                                                                              >  1:4254015/1‑150 (MQ=255)
                       atTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATcc                                                                                                                             >  2:2744483/1‑150 (MQ=255)
                           ccGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTc                                                                                                                         <  1:2849154/150‑1 (MQ=255)
                           ccGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTc                                                                                                                         <  1:4505114/150‑1 (MQ=255)
                             gTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCaa                                                                                                                       >  1:1329688/1‑150 (MQ=255)
                                ggCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACgg                                                                                                                    >  1:2584098/1‑150 (MQ=255)
                                 gCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGga                                                                                                                   <  1:2495242/150‑1 (MQ=255)
                                   aTCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgaga                                                                                                                 <  2:3899398/150‑1 (MQ=255)
                                   aTCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgaga                                                                                                                 >  2:590783/1‑150 (MQ=255)
                                   aTCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgaga                                                                                                                 <  2:5127804/150‑1 (MQ=255)
                                   aTCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGgaga                                                                                                                 >  1:3935594/1‑150 (MQ=255)
                                      ggAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGAt                                                                                                              >  1:2419273/1‑150 (MQ=255)
                                      ggAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGAt                                                                                                              >  1:2419138/1‑150 (MQ=255)
                                       gagaGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATg                                                                                                             <  2:487714/150‑1 (MQ=255)
                                        agagCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATgg                                                                                                            >  2:5093222/1‑150 (MQ=255)
                                        agagCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATgg                                                                                                            <  1:665436/150‑1 (MQ=255)
                                          agCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCa                                                                                                          <  1:323432/150‑1 (MQ=255)
                                             tCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATAc                                                                                                       <  1:1040202/150‑1 (MQ=255)
                                             tCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATAc                                                                                                       <  2:2519567/150‑1 (MQ=255)
                                             tCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATAc                                                                                                       >  2:4082477/1‑150 (MQ=255)
                                             tCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATAc                                                                                                       <  1:4106577/150‑1 (MQ=255)
                                                  ggTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGccc                                                                                                  <  1:1096997/150‑1 (MQ=255)
                                                  ggTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGccc                                                                                                  <  1:3571147/150‑1 (MQ=255)
                                                    tCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGccccc                                                                                                <  2:314945/150‑1 (MQ=255)
                                                    tCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGccccc                                                                                                >  1:3587265/1‑150 (MQ=255)
                                                      aGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCAc                                                                                              <  1:2345909/150‑1 (MQ=255)
                                                       gCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACt                                                                                             >  2:57529/1‑150 (MQ=255)
                                                        ccTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTc                                                                                            <  1:3001495/150‑1 (MQ=255)
                                                         cTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCa                                                                                           >  2:1758108/1‑150 (MQ=255)
                                                            tCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATc                                                                                        <  2:2986010/150‑1 (MQ=255)
                                                            tCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATc                                                                                        <  2:4200718/150‑1 (MQ=255)
                                                             cAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATcc                                                                                       <  1:1321681/150‑1 (MQ=255)
                                                             cAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATcc                                                                                       >  2:944602/1‑150 (MQ=255)
                                                             cAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATcc                                                                                       >  2:3695481/1‑150 (MQ=255)
                                                              aGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATccc                                                                                      <  2:47348/150‑1 (MQ=255)
                                                                  ttGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGc                                                                                  >  1:2156661/1‑150 (MQ=255)
                                                                   tGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCt                                                                                 <  1:1375280/150‑1 (MQ=255)
                                                                     aTACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTta                                                                               <  1:2907341/150‑1 (MQ=255)
                                                                      tACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTtac                                                                              <  1:4043207/150‑1 (MQ=255)
                                                                       aCCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTtact                                                                             <  2:4430127/150‑1 (MQ=255)
                                                                              aaCACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTgg                                                                      >  2:3874195/1‑150 (MQ=255)
                                                                                   gCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGttt                                                                 >  2:2395438/1‑150 (MQ=255)
                                                                                     ccTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTa                                                               >  1:3268009/1‑150 (MQ=255)
                                                                                      cTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTAt                                                              <  1:2009603/150‑1 (MQ=255)
                                                                                       tGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATc                                                             >  2:372341/1‑150 (MQ=255)
                                                                                       tGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATc                                                             <  2:1476527/150‑1 (MQ=255)
                                                                                       tGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATc                                                             >  2:2983127/1‑150 (MQ=255)
                                                                                        ggATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCa                                                            <  1:1917240/150‑1 (MQ=255)
                                                                                        ggATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCa                                                            <  1:4504782/150‑1 (MQ=255)
                                                                                              cGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCAt                                                      <  2:842893/150‑1 (MQ=255)
                                                                                                  cGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATcgcg                                                  >  1:2960800/1‑150 (MQ=255)
                                                                                                   gCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCgg                                                 <  1:590790/150‑1 (MQ=255)
                                                                                                        cATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGc                                            <  1:1030835/150‑1 (MQ=255)
                                                                                                         aTCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCACCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCa                                           >  1:271184/1‑150 (MQ=255)
                                                                                                            aGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCAttt                                        <  1:4607614/150‑1 (MQ=255)
                                                                                                              tGCCACGGCATCATCAAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTg                                      >  2:2028020/1‑150 (MQ=255)
                                                                                                               gCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGc                                     <  2:3305904/150‑1 (MQ=255)
                                                                                                                 cACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCAc                                   <  2:1599810/150‑1 (MQ=255)
                                                                                                                  aCGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCAcc                                  <  2:3587265/150‑1 (MQ=255)
                                                                                                                  aCGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCAcc                                  >  2:2428187/1‑150 (MQ=255)
                                                                                                                  aCGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCAcc                                  >  2:5008582/1‑150 (MQ=255)
                                                                                                                     gCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATg                               <  2:3417479/150‑1 (MQ=255)
                                                                                                                      catcatCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGc                              >  2:2562936/1‑150 (MQ=255)
                                                                                                                        tcatcaAGTAACGCGTTGCATTTACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGccc                            <  2:40807/150‑1 (MQ=255)
                                                                                                                         catcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCa                           >  2:3152686/1‑150 (MQ=255)
                                                                                                                         catcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCa                           >  1:3407862/1‑150 (MQ=255)
                                                                                                                         catcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCa                           >  1:2152818/1‑150 (MQ=255)
                                                                                                                          atcaAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCaa                          >  1:316273/1‑150 (MQ=255)
                                                                                                                            caAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTAACCGTTCAACGGAGAGATGGCATACAGCCCCCACTAAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAg                        >  2:342288/1‑150 (MQ=255)
                                                                                                                              aGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGaa                      <  2:1694349/150‑1 (MQ=255)
                                                                                                                                 aaCGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGa                   <  2:1173030/150‑1 (MQ=255)
                                                                                                                                 aaCGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGa                   >  1:1316424/1‑150 (MQ=255)
                                                                                                                                   cgcgTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATa                 >  2:3080282/1‑150 (MQ=255)
                                                                                                                                     cgTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAg               <  1:1467777/150‑1 (MQ=255)
                                                                                                                                      tttGCATTTAACGATTTCGAGGATCGCCAGGCTGTATCAGTTCATCGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGATGCTTTTTGCACCATGCCCATAGAATTATAAgg              <  1:91174/149‑1 (MQ=255)
                                                                                                                                      gTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAgg              <  1:2707624/150‑1 (MQ=255)
                                                                                                                                       ttGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGc             <  2:43386/150‑1 (MQ=255)
                                                                                                                                       ttGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGc             >  2:1959058/1‑150 (MQ=255)
                                                                                                                                       ttGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGc             <  2:3090354/150‑1 (MQ=255)
                                                                                                                                           aTTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGa         >  1:2632369/1‑150 (MQ=255)
                                                                                                                                           aTTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGa         >  1:4980356/1‑150 (MQ=255)
                                                                                                                                              tACCTATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGAAAt      >  1:5047650/1‑150 (MQ=255)
                                                                                                                                               aCCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGAAATa     >  1:401354/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGTTCCTGATAACTCAGTACAATATTGCCGTCGGCATCGGAGAGCTCAATGGTCAGCCTTTCAGGATTGATACCGTGCAACACGCCCTGGATGGCGGTCGCAGGCATCAGTGCCACGGCATCATCAAGTAACGCGTTGCATTTACCGATTTCGCGGATCGCCAGGCGGTATCCGTTCAACGGAGAGATGGCATACAGCCCCCACTCAATCCCCCGCTTACTACGCTGGAGTTTTATCACCGCATCGCGGGAGGCATTTTGCACCATGCCCAAAGAATGATAAGGCAGGAAATACTG  >  CP009273/2674795‑2675090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A