Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,103,319 C→T 19.7% I136I (ATC→ATT)  yqgA → DUF554 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,103,3190CT19.7% 741.1 / 163.3 300I136I (ATC→ATT) yqgADUF554 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (123/118);  new base T (27/32);  total (150/150)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 308 total aligned reads displayed.

CAAAATCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGAT  >  CP009273/3103170‑3103468
                                                                                                                                                     |                                                                                                                                                     
caAAATCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATc                                                                                                                                                       <  2:2035971/150‑1 (MQ=255)
caAAATCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATc                                                                                                                                                       >  1:2384472/1‑150 (MQ=255)
 aaaaTCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCg                                                                                                                                                      >  2:87834/1‑150 (MQ=255)
   aaTCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGcc                                                                                                                                                    <  2:1590212/150‑1 (MQ=255)
   aaTCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGcc                                                                                                                                                    >  1:1503490/1‑150 (MQ=255)
     tCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCaa                                                                                                                                                  >  2:3414223/1‑150 (MQ=255)
     tCTGTTACGTCACACACGTAAGAAGCCAGCGCATGAATCTGTTATTCAGAAATATGTCGCGAGAATTGTCCTCAATTGCGCCAGCGGCACCGGGATCTACGGGGCGAGGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCaa                                                                                                                                                  >  2:2485574/1‑150 (MQ=255)
       tGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGt                                                                                                                                                >  1:250168/1‑150 (MQ=255)
       tGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTGATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGt                                                                                                                                                <  1:653513/150‑1 (MQ=255)
         tttCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCTGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCa                                                                                                                                              <  2:1215649/150‑1 (MQ=255)
           tCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCAtt                                                                                                                                            <  2:4390730/150‑1 (MQ=255)
           tCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCAtt                                                                                                                                            <  2:2972129/150‑1 (MQ=255)
           tCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCAtt                                                                                                                                            <  2:3883592/150‑1 (MQ=255)
            cGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCAttt                                                                                                                                           <  1:2767472/150‑1 (MQ=255)
             gTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTc                                                                                                                                          >  1:352800/1‑150 (MQ=255)
              taactcacGTAAGAAGCCAGCGCATGAATATTTTATTCATAATTATGTCGCGATTTTTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGCGGCGATGATCGATGGGATGTCCTGCGATCCTTTTATTTTTATTGCCAAGTCATTTCt                                                                                                                                         <  2:3633569/148‑1 (MQ=255)
               cactcacGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTATTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCtt                                                                                                                                        <  2:3825283/150‑1 (MQ=255)
                   cacGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGAtt                                                                                                                                    <  1:1327821/150‑1 (MQ=255)
                   cacGTAAGAAGCCAGCGCATGAATCTTTGATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGAtt                                                                                                                                    <  1:341996/150‑1 (MQ=255)
                    acGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGAttt                                                                                                                                   <  2:2452072/150‑1 (MQ=255)
                     cGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTc                                                                                                                                  <  1:1078992/150‑1 (MQ=255)
                         agaagCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTa                                                                                                                              <  1:479440/150‑1 (MQ=255)
                         agaagCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTa                                                                                                                              <  1:744921/150‑1 (MQ=255)
                          gaagCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTAc                                                                                                                             <  1:3368554/150‑1 (MQ=255)
                            agCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACgg                                                                                                                           >  1:917504/1‑150 (MQ=255)
                               cAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCga                                                                                                                        >  1:4691992/1‑150 (MQ=255)
                                    cATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATc                                                                                                                   >  1:1157296/1‑150 (MQ=255)
                                      tGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCtt                                                                                                                 <  1:4131174/150‑1 (MQ=255)
                                      tGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCtt                                                                                                                 <  1:1521473/150‑1 (MQ=255)
                                       gAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTc                                                                                                                >  1:3004914/1‑150 (MQ=255)
                                       gAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTc                                                                                                                <  1:2263901/150‑1 (MQ=255)
                                          tCTTTTATTCAGAATTATGTCTCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGcc                                                                                                             <  2:4040243/150‑1 (MQ=255)
                                               tATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTc                                                                                                        >  2:1707458/1‑150 (MQ=255)
                                               tATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTc                                                                                                        <  2:685889/150‑1 (MQ=255)
                                               tATTCAGAATTATGTCGCGATTAGTGTCCTGTTTTGCGCCAGCGGCACCGGGAACTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTc                                                                                                        >  2:147863/1‑150 (MQ=255)
                                                 ttCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGc                                                                                                      >  1:5091991/1‑150 (MQ=255)
                                                   cAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTg                                                                                                    <  1:979862/150‑1 (MQ=255)
                                                   cAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTg                                                                                                    >  1:2638402/1‑150 (MQ=255)
                                                       aTTCTGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTa                                                                                                <  2:2090115/150‑1 (MQ=255)
                                                       aTTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACTGCGATGATCTTCGCCTGCTCGCTgggga                                                                                                >  2:3214561/1‑148 (MQ=255)
                                                       aTTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTa                                                                                                <  1:1691074/150‑1 (MQ=255)
                                                       aTTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTa                                                                                                >  1:1488967/1‑150 (MQ=255)
                                                         tATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTAtt                                                                                              >  1:2432615/1‑150 (MQ=255)
                                                         tATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTAtt                                                                                              <  1:4285000/150‑1 (MQ=255)
                                                            gTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGAGGATCTTCGCCTGCTCGCTGGGTAGTGCg                                                                                           >  2:2730946/1‑150 (MQ=255)
                                                             tCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCgg                                                                                          >  2:4468827/1‑150 (MQ=255)
                                                               gcgATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTa                                                                                        <  1:85625/150‑1 (MQ=255)
                                                                cgATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTAt                                                                                       >  1:1386489/1‑150 (MQ=255)
                                                                cgATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTAAGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTAt                                                                                       >  2:2984132/1‑150 (MQ=255)
                                                                cgATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTAt                                                                                       <  2:5143143/150‑1 (MQ=255)
                                                                cgATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTAt                                                                                       <  2:2310810/150‑1 (MQ=255)
                                                                      ttGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTCTTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGa                                                                                 >  2:355962/1‑150 (MQ=255)
                                                                      ttGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGa                                                                                 <  2:4607254/150‑1 (MQ=255)
                                                                      ttGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGa                                                                                 >  2:3456308/1‑150 (MQ=255)
                                                                      ttGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGa                                                                                 >  2:830864/1‑150 (MQ=255)
                                                                        gTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTCTTTTACTTGCCAAGTCATTTCGTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGGATTGCGGTATCGGTGAtt                                                                               >  2:1331530/1‑150 (MQ=255)
                                                                            tGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTa                                                                           <  1:1591427/150‑1 (MQ=255)
                                                                             gTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTAt                                                                          <  1:5225677/150‑1 (MQ=255)
                                                                              ttttGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATc                                                                         >  1:3239171/1‑150 (MQ=255)
                                                                              ttttGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATc                                                                         <  1:1963883/150‑1 (MQ=255)
                                                                                 tGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCa                                                                      >  2:568291/1‑150 (MQ=255)
                                                                                  gcgcCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCAt                                                                     <  2:4232970/150‑1 (MQ=255)
                                                                                    gcCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTa                                                                   <  2:3046419/150‑1 (MQ=255)
                                                                                      cAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACt                                                                 <  1:4750692/150‑1 (MQ=255)
                                                                                       aGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTg                                                                <  2:3971110/150‑1 (MQ=255)
                                                                                         cGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGat                                                              <  2:1269655/150‑1 (MQ=255)
                                                                                          ggCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGatc                                                             >  2:1977278/1‑150 (MQ=255)
                                                                                           gCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGatca                                                            >  2:4172407/1‑150 (MQ=255)
                                                                                            cACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGatcat                                                           <  2:522826/150‑1 (MQ=255)
                                                                                            cACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGatcat                                                           >  2:606693/1‑150 (MQ=255)
                                                                                             aCCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGatcatc                                                          >  2:758200/1‑150 (MQ=255)
                                                                                             aCCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGAGTAGTATCCCATTACTGatcatc                                                          >  2:3383080/1‑150 (MQ=255)
                                                                                              ccGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATcc                                                         <  2:1123291/150‑1 (MQ=255)
                                                                                               cGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCa                                                        >  2:3921066/1‑150 (MQ=255)
                                                                                                 ggATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGt                                                      <  2:4318666/150‑1 (MQ=255)
                                                                                                   aTCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTa                                                    <  2:1514791/150‑1 (MQ=255)
                                                                                                     cTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAAc                                                  >  2:3282577/1‑150 (MQ=255)
                                                                                                         ggggCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTg                                              <  1:4356091/150‑1 (MQ=255)
                                                                                                          gggCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGTTCATCCTGTTAACGCTgg                                             >  2:2851170/1‑150 (MQ=255)
                                                                                                           ggCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGc                                            <  2:5237971/150‑1 (MQ=255)
                                                                                                           ggCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCAGCCAGTTAACGCTGGc                                            <  2:5010275/150‑1 (MQ=255)
                                                                                                               aTGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTgg                                        >  1:4668756/1‑150 (MQ=255)
                                                                                                                  aaCGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCt                                     >  2:2374434/1‑150 (MQ=255)
                                                                                                                   aCGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTg                                    >  1:2769797/1‑150 (MQ=255)
                                                                                                                            tGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCGGa                           >  2:5166579/1‑150 (MQ=255)
                                                                                                                             gACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGAt                          >  1:1902209/1‑150 (MQ=255)
                                                                                                                                cGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGGTCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGTTGGCGTGGGCTGCCGCGCTGAtttt                       >  2:2254106/1‑150 (MQ=255)
                                                                                                                                 ggCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTa                      >  1:4620138/1‑150 (MQ=255)
                                                                                                                                 ggCGATCCGAGTATTTTAATCGCCAAGTCATTTCGTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGGAGTGCGGGATCGGTGATTTGTAGCCCATTACTGATCATCCAGTTCACGCTGGCGGGGGCTGCCGAGCTGAGTTTa                      >  2:680362/1‑150 (MQ=255)
                                                                                                                                   cGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTAcc                    <  1:4009868/150‑1 (MQ=255)
                                                                                                                                         gAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGAc              >  1:4981044/1‑150 (MQ=255)
                                                                                                                                          aGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGAcc             >  2:1753739/1‑150 (MQ=255)
                                                                                                                                           gTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACca            >  1:5217158/1‑150 (MQ=255)
                                                                                                                                            tATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACcac           <  1:3937616/150‑1 (MQ=255)
                                                                                                                                               tttAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACAcc        <  2:1848412/150‑1 (MQ=255)
                                                                                                                                               tttAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACAcc        <  1:4505803/150‑1 (MQ=255)
                                                                                                                                                 tAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGt      >  1:2563901/1‑150 (MQ=255)
                                                                                                                                                  aaTTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTc     >  1:3742091/1‑150 (MQ=255)
                                                                                                                                                  aaTTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTc     >  1:1297395/1‑150 (MQ=255)
                                                                                                                                                     cGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCgat  >  1:3901706/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAAAATCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATCGGTGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGAT  >  CP009273/3103170‑3103468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A