Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,103,319 C→T 27.5% I136I (ATC→ATT)  yqgA → DUF554 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,103,3190CT27.5% 584.0 / 263.2 323I136I (ATC→ATT) yqgADUF554 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (135/99);  new base T (48/41);  total (183/140)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 324 total aligned reads displayed.

CAAAATCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGA  >  CP009273/3103170‑3103467
                                                                                                                                                     |                                                                                                                                                      
caAAATCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATc                                                                                                                                                        <  2:1094026/150‑1 (MQ=255)
 aaaaTCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCg                                                                                                                                                       <  2:3899323/150‑1 (MQ=255)
 aaaaTCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCg                                                                                                                                                       >  2:3114393/1‑150 (MQ=255)
        gTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTc                                                                                                                                                <  1:752386/150‑1 (MQ=255)
         tttCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCa                                                                                                                                               <  1:843649/150‑1 (MQ=255)
         tttCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCa                                                                                                                                               <  1:2787948/150‑1 (MQ=255)
             gTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTc                                                                                                                                           <  1:3292967/150‑1 (MQ=255)
                actcacGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTg                                                                                                                                        <  1:461421/150‑1 (MQ=255)
                    acGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGAttt                                                                                                                                    <  1:1942241/150‑1 (MQ=255)
                    acGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGAttt                                                                                                                                    <  1:4394432/150‑1 (MQ=255)
                     cGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTc                                                                                                                                   <  2:4296055/150‑1 (MQ=255)
                     cGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTc                                                                                                                                   <  2:2167313/150‑1 (MQ=255)
                     cGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTc                                                                                                                                   >  1:3962888/1‑150 (MQ=255)
                      gTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCt                                                                                                                                  >  2:948373/1‑150 (MQ=255)
                      gTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCt                                                                                                                                  <  2:306822/150‑1 (MQ=255)
                        aagaagCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCttt                                                                                                                                <  2:1275891/150‑1 (MQ=255)
                        aagaagCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCttt                                                                                                                                >  2:769217/1‑150 (MQ=255)
                         agaagCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTa                                                                                                                               <  1:121607/150‑1 (MQ=255)
                         agaagCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTCAAAAGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTa                                                                                                                               >  2:4423973/1‑150 (MQ=255)
                           aagCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACg                                                                                                                             <  2:4790002/150‑1 (MQ=255)
                           aagCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACg                                                                                                                             <  2:3446101/150‑1 (MQ=255)
                              ccAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCg                                                                                                                          <  2:1005679/150‑1 (MQ=255)
                               cAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCga                                                                                                                         >  2:896639/1‑150 (MQ=255)
                               cAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCga                                                                                                                         >  2:2526743/1‑150 (MQ=255)
                               cAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCTCCAAGTCATTTCTTGATTTCTTTACGGCga                                                                                                                         >  1:1808855/1‑150 (MQ=255)
                                aGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCgat                                                                                                                        >  1:2900208/1‑150 (MQ=255)
                                  cgcATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCgatga                                                                                                                      <  2:1405477/150‑1 (MQ=255)
                                   gcATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCgatgat                                                                                                                     <  1:1580081/150‑1 (MQ=255)
                                    cATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATc                                                                                                                    <  1:4043571/150‑1 (MQ=255)
                                      tGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCtt                                                                                                                  <  1:534899/150‑1 (MQ=255)
                                           cTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCt                                                                                                             <  2:2897623/150‑1 (MQ=255)
                                               tATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTc                                                                                                         >  2:1223569/1‑150 (MQ=255)
                                               tATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTc                                                                                                         <  1:1481155/150‑1 (MQ=255)
                                               tATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTc                                                                                                         <  2:3265568/150‑1 (MQ=255)
                                               tATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTc                                                                                                         <  2:461419/150‑1 (MQ=255)
                                                  tCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCt                                                                                                      <  2:2143975/150‑1 (MQ=255)
                                                    aGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTgg                                                                                                    <  2:3622377/150‑1 (MQ=255)
                                                    aGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTgg                                                                                                    >  2:4217869/1‑150 (MQ=255)
                                                      aaTTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGt                                                                                                  >  1:714646/1‑150 (MQ=255)
                                                       aTTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTa                                                                                                 >  2:809423/1‑150 (MQ=255)
                                                           tGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGc                                                                                             >  2:4871062/1‑150 (MQ=255)
                                                              cgcgATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGt                                                                                          <  1:2160469/150‑1 (MQ=255)
                                                               gcgATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTa                                                                                         >  2:261484/1‑150 (MQ=255)
                                                                cgATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTAt                                                                                        <  1:1534045/150‑1 (MQ=255)
                                                                cgATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTAt                                                                                        >  1:4279713/1‑150 (MQ=255)
                                                                   ttattGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑gg                                                                                    >  2:4483998/1‑150 (MQ=255)
                                                                   ttattGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑gg                                                                                    >  2:4482726/1‑150 (MQ=255)
                                                                      ttGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGa                                                                                >  1:3786844/1‑150 (MQ=255)
                                                                      ttGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGa                                                                                >  1:2006202/1‑150 (MQ=255)
                                                                       tGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGAt                                                                               <  2:4119252/150‑1 (MQ=255)
                                                                         tCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTa                                                                             >  2:3359797/1‑150 (MQ=255)
                                                                          ccTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAg                                                                            <  1:3438482/150‑1 (MQ=255)
                                                                          ccTGTTTTGCGCCAGCGGCACCGGGATCGTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAg                                                                            <  2:2454472/150‑1 (MQ=255)
                                                                           cTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGt                                                                           >  1:2254391/1‑150 (MQ=255)
                                                                             gTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTAt                                                                         >  1:4762298/1‑150 (MQ=255)
                                                                             gTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTAt                                                                         <  2:1632751/150‑1 (MQ=255)
                                                                             gTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTAt                                                                         >  2:4726147/1‑150 (MQ=255)
                                                                                  gcgcCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCAt                                                                    <  2:1660941/150‑1 (MQ=255)
                                                                                   cgcCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCAtt                                                                   <  2:3314604/150‑1 (MQ=255)
                                                                                    gcCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTa                                                                  >  1:48363/1‑150 (MQ=255)
                                                                                     ccAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTAc                                                                 <  1:3178449/150‑1 (MQ=255)
                                                                                       aGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTg                                                               >  1:4256522/1‑150 (MQ=255)
                                                                                       aGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTg                                                               <  1:3805214/150‑1 (MQ=255)
                                                                                         cGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGat                                                             <  1:2222732/150‑1 (MQ=255)
                                                                                         cGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATAGCGGTATC‑GG‑TGATTAGTATCCCATTACAGat                                                             >  1:3647111/1‑150 (MQ=255)
                                                                                          ggCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGatc                                                            >  2:2724159/1‑150 (MQ=255)
                                                                                            cACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGatcat                                                          >  1:3837712/1‑150 (MQ=255)
                                                                                                gggATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAg                                                      >  2:2977058/1‑150 (MQ=255)
                                                                                                 ggATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGt                                                     >  1:604519/1‑150 (MQ=255)
                                                                                                  gATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGtt                                                    <  1:1916861/150‑1 (MQ=255)
                                                                                                      ttCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACg                                                >  1:1054058/1‑150 (MQ=255)
                                                                                                        cGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCt                                              <  2:3993038/150‑1 (MQ=255)
                                                                                                        cGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCt                                              >  2:1193236/1‑150 (MQ=255)
                                                                                                           ggCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGc                                           >  1:3125255/1‑150 (MQ=255)
                                                                                                            gCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCg                                          <  2:2021190/150‑1 (MQ=255)
                                                                                                             cGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGt                                         >  2:2501172/1‑150 (MQ=255)
                                                                                                              gATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTg                                        <  1:2539743/150‑1 (MQ=255)
                                                                                                                tGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTggg                                      >  2:2262383/1‑150 (MQ=255)
                                                                                                                  aaCGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCt                                    >  1:3964197/1‑150 (MQ=255)
                                                                                                                   aCGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTg                                   >  2:2048771/1‑150 (MQ=255)
                                                                                                                    cGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGc                                  >  2:4183052/1‑150 (MQ=255)
                                                                                                                     gAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGcc                                 <  1:1675892/150‑1 (MQ=255)
                                                                                                                     gAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGcc                                 >  1:1156065/1‑150 (MQ=255)
                                                                                                                         ggATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCgcgc                             >  1:4569464/1‑150 (MQ=255)
                                                                                                                           aTGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTg                           <  2:1970874/150‑1 (MQ=255)
                                                                                                                            tGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGa                          >  2:4723121/1‑150 (MQ=255)
                                                                                                                             gACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGAt                         <  2:3740701/150‑1 (MQ=255)
                                                                                                                               ccGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGTTAACTGGATGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGAt                         <  1:2389495/150‑1 (MQ=255)
                                                                                                                               ccGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGAttt                       >  2:1711514/1‑150 (MQ=255)
                                                                                                                                   cGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTAcc                   >  2:1568129/1‑150 (MQ=255)
                                                                                                                                   cGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTAcc                   >  2:3233912/1‑150 (MQ=255)
                                                                                                                                    gATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCg                  >  1:4124338/1‑150 (MQ=255)
                                                                                                                                    gATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCg                  >  2:1914213/1‑150 (MQ=255)
                                                                                                                                      tCCGAGTATTTTAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCt                <  1:3062613/150‑1 (MQ=255)
                                                                                                                                       ccGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTg               >  1:1420322/1‑150 (MQ=255)
                                                                                                                                               tttAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACAcc       <  1:3336440/150‑1 (MQ=255)
                                                                                                                                               tttAATTGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACAcc       <  1:896639/150‑1 (MQ=255)
                                                                                                                                                   aTCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCg   >  1:775859/1‑150 (MQ=255)
                                                                                                                                                   aTCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCg   <  1:2467526/150‑1 (MQ=255)
                                                                                                                                                    tCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCga  <  1:4217869/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CAAAATCTGTTTCGTCACTCACGTAAGAAGCCAGCGCATGAATCTTTTATTCAGAATTATGTCGCGATTATTGTCCTGTTTTGCGCCAGCGGCACCGGGATCTTCGGGGCGATGAACGAAGGGATGACCGGCGATCCGAGTATTTTAATCGCCAAGTCATTTCTTGATTTCTTTACGGCGATGATCTTCGCCTGCTCGCTGGGTATTGCGGTATC‑GG‑TGATTAGTATCCCATTACTGATCATCCAGTTAACGCTGGCGTGGGCTGCCGCGCTGATTTTACCGCTGACCACACCGTCGA  >  CP009273/3103170‑3103467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A