Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,732,014 C→A 8.3% intergenic (‑212/‑87) ynhF ← / → purR stress response membrane/transcriptional repressor, hypoxanthine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,732,0140CA 8.3% 672.2 / 33.9 192intergenic (‑212/‑87)ynhF/purRstress response membrane/transcriptional repressor, hypoxanthine‑binding
Reads supporting (aligned to +/- strand):  ref base C (100/76);  new base A (8/8);  total (108/84)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

Only 100 of 200 total aligned reads displayed.

TGAAAAAATCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTG  >  CP009273/1731865‑1732163
                                                                                                                                                     |                                                                                                                                                     
tgaAAAAATCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAac                                                                                                                                                       <  1:4071143/150‑1 (MQ=255)
 gaAAAAATCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAaca                                                                                                                                                      >  1:1343035/1‑150 (MQ=255)
 gaAAAAATCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAaca                                                                                                                                                      <  1:1737514/150‑1 (MQ=255)
   taaaaTCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAAAACt                                                                                                                                                    <  1:4295115/149‑1 (MQ=255)
    aaaaTCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCACCACtt                                                                                                                                                   >  2:1281000/1‑150 (MQ=255)
      aaTCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACtttt                                                                                                                                                 >  1:2390166/1‑150 (MQ=255)
       aTCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTttg                                                                                                                                                >  1:2325655/1‑150 (MQ=255)
        tctcCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTttgt                                                                                                                                               <  1:411423/150‑1 (MQ=255)
         ctcCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTttgtt                                                                                                                                              <  2:4412612/150‑1 (MQ=255)
            ccGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTtgtg                                                                                                                                           <  1:2736820/150‑1 (MQ=255)
             cGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGc                                                                                                                                          <  1:845586/150‑1 (MQ=255)
                cATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTa                                                                                                                                       >  1:4488532/1‑150 (MQ=255)
                cATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTa                                                                                                                                       <  2:594279/150‑1 (MQ=255)
                 aTTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTGCGGCGTACCGCAACACTTTTGTTGTGCGTaa                                                                                                                                      >  1:4058033/1‑150 (MQ=255)
                  tttATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAAAACTTTTGTTGTGCGTAAg                                                                                                                                     <  2:4007028/150‑1 (MQ=255)
                   ttATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAgg                                                                                                                                    <  2:3524477/150‑1 (MQ=255)
                    tataATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGgt                                                                                                                                   >  1:663781/1‑150 (MQ=255)
                    tataATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACATTTTTTGTGCGTAAGgt                                                                                                                                   >  1:425464/1‑150 (MQ=255)
                        aTGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGgtgtgt                                                                                                                               >  2:1747421/1‑150 (MQ=255)
                         tGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTa                                                                                                                              <  2:2469715/150‑1 (MQ=255)
                          gATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACACCTTCATGACGCTCCGTGTCGTTTTTACGGCGTACCGAAACACTTTTGTTGTGCGTAAGGTGTGTaa                                                                                                                             >  2:2359247/1‑150 (MQ=255)
                              aGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGc                                                                                                                         <  2:3699761/150‑1 (MQ=255)
                                     ttACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGtt                                                                                                                  >  2:383592/1‑150 (MQ=255)
                                     ttACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGtt                                                                                                                  >  2:3698894/1‑150 (MQ=255)
                                        ccACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTCACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTAc                                                                                                               <  1:513380/150‑1 (MQ=255)
                                         cACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTAcc                                                                                                              >  2:1058248/1‑150 (MQ=255)
                                         cACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTAcc                                                                                                              <  1:2022740/150‑1 (MQ=255)
                                          aCTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCt                                                                                                             <  2:1298081/150‑1 (MQ=255)
                                          aCTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCt                                                                                                             <  2:1402348/150‑1 (MQ=255)
                                          aCTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCt                                                                                                             <  2:3691011/150‑1 (MQ=255)
                                          aCTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCt                                                                                                             >  2:1912352/1‑150 (MQ=255)
                                           cgtCCCCTTGTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCtt                                                                                                            <  2:2619255/148‑1 (MQ=255)
                                            ttCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTg                                                                                                           <  1:1146093/150‑1 (MQ=255)
                                                ccTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCCAACGTTTACCTTGCGAt                                                                                                       >  1:2142009/1‑150 (MQ=255)
                                                    ttCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTg                                                                                                   <  1:3934885/150‑1 (MQ=255)
                                                    ttCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTg                                                                                                   <  2:68372/150‑1 (MQ=255)
                                                        tCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAgg                                                                                               <  2:788304/150‑1 (MQ=255)
                                                          aaGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGGGTGTAAAGGCAAACGTTTACCGTGCGATTTTGCAGGAg                                                                                             >  1:3395474/1‑150 (MQ=255)
                                                           aGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTTTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGc                                                                                            >  1:84052/1‑150 (MQ=255)
                                                           aGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTACGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAAAGTTTACCTTGCGATTTTTCAGGAGc                                                                                            >  1:3173580/1‑150 (MQ=255)
                                                              tCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGa                                                                                         <  1:1462093/150‑1 (MQ=255)
                                                               cGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGaa                                                                                        <  1:885774/150‑1 (MQ=255)
                                                                 gCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAgg                                                                                      >  2:1188937/1‑149 (MQ=255)
                                                                 gCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTGTTGTTGTGCGGAAGGTGTGTAAAGGCCAACGTTTACCTTGCGATTTTGCAGGAGCGGAAGt                                                                                      >  2:1191368/1‑150 (MQ=255)
                                                                   cAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTa                                                                                    >  1:4374461/1‑150 (MQ=255)
                                                                    aacaTTCCACGCGTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAg                                                                                   <  2:2861048/147‑1 (MQ=255)
                                                                     aaaTTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAgg                                                                                  >  1:1855472/1‑150 (MQ=255)
                                                                         tCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCt                                                                              <  2:3118989/150‑1 (MQ=255)
                                                                         tCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGATTACCATGCGATTTTTCAGGAGCTGAAGTTATGGTCt                                                                              >  2:2604429/1‑150 (MQ=255)
                                                                          ccACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTg                                                                             >  2:2656454/1‑150 (MQ=255)
                                                                              gCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAAAACTTTTGTTGTGCGTAAGGTGTGTAAATGCAAACGTTTAACTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGt                                                                         >  2:1232255/1‑150 (MQ=255)
                                                                              gCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAAAACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGt                                                                         <  1:2309152/150‑1 (MQ=255)
                                                                                  acacTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGaaa                                                                     >  2:3462142/1‑150 (MQ=255)
                                                                                  acacTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGaaa                                                                     >  1:4102582/1‑150 (MQ=255)
                                                                                     cTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAaatgg                                                                  <  1:1303904/150‑1 (MQ=255)
                                                                                     cTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAaatgg                                                                  >  2:4732097/1‑150 (MQ=255)
                                                                                      tATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAaatgga                                                                 <  2:2528550/150‑1 (MQ=255)
                                                                                      tATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAAAACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAaatgga                                                                 >  1:2578583/1‑150 (MQ=255)
                                                                                      tATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAAAACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAaatgga                                                                 >  1:3308718/1‑150 (MQ=255)
                                                                                        tttGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTGTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAaatggaat                                                               <  1:2984979/150‑1 (MQ=255)
                                                                                          tGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAaatggaatgg                                                             >  2:4620968/1‑150 (MQ=255)
                                                                                            cGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGca                                                           >  2:1955845/1‑150 (MQ=255)
                                                                                              tACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGcaac                                                         <  1:2123870/150‑1 (MQ=255)
                                                                                                cTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGcaacaa                                                       <  1:3242780/150‑1 (MQ=255)
                                                                                                 tGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAAt                                                      <  2:4332924/150‑1 (MQ=255)
                                                                                                 tGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTAACGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCGTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGTGTGAAATGGAATGGCAACAAt                                                      >  1:1182935/1‑150 (MQ=255)
                                                                                                  ggCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATa                                                     <  2:4269969/150‑1 (MQ=255)
                                                                                                       ttGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGa                                                <  2:3340842/150‑1 (MQ=255)
                                                                                                             ccTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGc                                          <  2:4158850/150‑1 (MQ=255)
                                                                                                             ccTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAAAACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGc                                          <  1:491000/150‑1 (MQ=255)
                                                                                                             ccTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAAAACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGc                                          <  1:2861778/150‑1 (MQ=255)
                                                                                                                tCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGaa                                       >  2:619960/1‑150 (MQ=255)
                                                                                                                 ccTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGaaa                                      <  1:3862549/150‑1 (MQ=255)
                                                                                                                 ccTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGaaa                                      <  1:1469479/150‑1 (MQ=255)
                                                                                                                 ccTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGaaa                                      <  1:2720313/150‑1 (MQ=255)
                                                                                                                 ccTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAAAACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCACCAATAAAAGATGTAGCGaaa                                      >  2:290924/1‑150 (MQ=255)
                                                                                                                   tGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATTTAGCGaaaag                                    >  2:657799/1‑148 (MQ=255)
                                                                                                                   tGACGCTCCGTGTCGTTTTTACGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTTTAAATGCAAACGTTTAACATTCGATTTTTCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAAAAAAAGATTTAGCGaaaag                                    >  1:1596905/1‑148 (MQ=255)
                                                                                                                     aCGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAg                                  >  1:1925743/1‑150 (MQ=255)
                                                                                                                       gCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCa                                >  2:3876218/1‑150 (MQ=255)
                                                                                                                       gCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCa                                >  2:4695901/1‑150 (MQ=255)
                                                                                                                        cTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGGGTGTAAAGGCAAACGTTTACCTTGCGATTTTTCAGGAGCGGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTATCGAAAAGAGCaa                               >  2:3173564/1‑150 (MQ=255)
                                                                                                                         tCCGTGTCGTTTTTACGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTTCCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAAAAATAAAATATGTAGCGAAACGAGCaaa                              >  1:2267561/1‑150 (MQ=255)
                                                                                                                          ccGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAAc                             <  2:4210320/150‑1 (MQ=255)
                                                                                                                              gtCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGttt                         >  2:3411320/1‑150 (MQ=255)
                                                                                                                              gtCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGttt                         >  1:1424010/1‑150 (MQ=255)
                                                                                                                               tCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTc                        <  2:1640559/150‑1 (MQ=255)
                                                                                                                                 gTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCa                      >  2:2190228/1‑150 (MQ=255)
                                                                                                                                  tttttCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCAc                     >  1:4347099/1‑150 (MQ=255)
                                                                                                                                      tCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACa                 >  1:860176/1‑150 (MQ=255)
                                                                                                                                      tCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACCAGCAAACGTTTCCacaaca                 >  1:3859954/1‑150 (MQ=255)
                                                                                                                                           cGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACtgt            <  2:4278460/150‑1 (MQ=255)
                                                                                                                                            gTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACtgtg           >  1:1723696/1‑150 (MQ=255)
                                                                                                                                              aCCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTc         <  2:4058047/150‑1 (MQ=255)
                                                                                                                                              aCCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTc         >  1:3227138/1‑150 (MQ=255)
                                                                                                                                                cGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCACCAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTcac       >  1:606620/1‑150 (MQ=255)
                                                                                                                                                cGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTcac       <  1:3778904/150‑1 (MQ=255)
                                                                                                                                                  cAACACTTTAGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAATATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCacac     >  2:90228/1‑150 (MQ=255)
                                                                                                                                                    acacTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGt   <  1:1249455/150‑1 (MQ=255)
                                                                                                                                                     cacTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTg  >  1:3323542/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGAAAAAATCTCCCGTCATTTATAATGATAAGTGTTTTTACCACTTCCCCTTTTCGTCAAGATCGGCCAAAATTCCACGCTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTG  >  CP009273/1731865‑1732163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A