Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,730,313 G→A 23.9% intergenic (+71/‑200) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,730,3130GA23.9% 285.8 / 99.0 158intergenic (+71/‑200)bamD/raiABamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
Reads supporting (aligned to +/- strand):  ref base G (28/85);  new base A (14/23);  total (50/108)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

Only 100 of 159 total aligned reads displayed.

GCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACATTTTGACATC  >  CP009273/2730165‑2730462
                                                                                                                                                    |                                                                                                                                                     
gCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGc                                                                                                                                                      >  2:1359059/1‑150 (MQ=255)
   tACCGTCAGATGCAGTTGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGAAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGAt                                                                                                                                                   <  1:2933054/150‑1 (MQ=255)
     ccGTCAGATGCAGATGAAGGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAttt                                                                                                                                                 <  2:3011507/150‑1 (MQ=255)
      cGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTa                                                                                                                                                <  2:1517294/150‑1 (MQ=255)
      cGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTa                                                                                                                                                <  2:3908613/150‑1 (MQ=255)
      cGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTATGTCAGTTGTGAAAATGAATCGATTTa                                                                                                                                                >  2:674308/1‑150 (MQ=255)
       gTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACAGAAACGATTTat                                                                                                                                               >  2:3688120/1‑149 (MQ=255)
        tCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGt                                                                                                                                              >  2:611792/1‑150 (MQ=255)
           gatgcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAGTTAGTCGc                                                                                                                                           >  1:337291/1‑150 (MQ=255)
                agatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACATAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCg                                                                                                                                      <  2:2325512/150‑1 (MQ=255)
                agatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGACACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCg                                                                                                                                      <  2:2325576/150‑1 (MQ=255)
                  atgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCCCAAACAGCACCAATACATAACCGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTATTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAt                                                                                                                                    <  2:4099621/150‑1 (MQ=255)
                    gAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAAATGAAGCGATTTAGTCGCTATCGctct                                                                                                                                  >  2:1442419/1‑150 (MQ=255)
                      aggcgcAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtca                                                                                                                                <  2:1657594/148‑1 (MQ=255)
                      aTGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTGTTTTTTTTGTGTGAGTTTTGTAAATGTACCCATTTTGTGGCTATCGAtgaga                                                                                                                                >  1:2899574/1‑146 (MQ=255)
                      aTGCGCAAGCTCAAAAAGTAGCGAAAATCCTCGCCGCAAACAGCAGCAATACATAACACAAACCTGAAACACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtca                                                                                                                                <  2:4206964/150‑1 (MQ=255)
                       tGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTATGTCAGGTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtcat                                                                                                                               >  2:2141012/1‑150 (MQ=255)
                       tGCGAAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATACAAGAAACCTCAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtcat                                                                                                                               <  1:1018211/150‑1 (MQ=255)
                            aaGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAat                                                                                                                          >  2:1845494/1‑150 (MQ=255)
                             aGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTGTTTTTTTTATGTCAGGTTTTTAAAAGAAAAGAATTAGTCGCGAACGATCACATCaaaaa                                                                                                                         >  1:3587661/1‑148 (MQ=255)
                              gCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAACCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAatat                                                                                                                        <  2:571453/150‑1 (MQ=255)
                              gCTGAAAAAGTAGCGAAAATCAGCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAatat                                                                                                                        <  2:2434424/150‑1 (MQ=255)
                               cttaaaaaTTAGCCAAAATCACCGCCGCAAACAGCACAAATACATAACATAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATg                                                                                                                       <  1:490978/147‑1 (MQ=255)
                               cTGAAAAAGTAGCTAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATg                                                                                                                       <  1:1063762/150‑1 (MQ=255)
                                tGAAAAAGAAGCGAACAACCCCGCCGCAAACACCCCAAATCCAAACCAGAACCCTCAACCACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATgg                                                                                                                      <  1:886520/150‑1 (MQ=255)
                                 gAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCATTTGTGAAACTGAAGCGATTTAGTAGCTATCGATCTCATCAAATATTGc                                                                                                                     >  2:2640070/1‑150 (MQ=255)
                                  aaaaaGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCt                                                                                                                    >  2:4718280/1‑150 (MQ=255)
                                    aaaTTAGCTAAAATCAACGCCGCAAACAGCAGCAATACAAAACATAACCCTTAAACACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCg                                                                                                                  <  1:530956/150‑1 (MQ=255)
                                    aaaGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAAATGAAGCGATTTAGTCGCAAGAGGTGTCATCAAAAATGGcgcg                                                                                                                  >  1:1852469/1‑150 (MQ=255)
                                      attAGCTAAAATCATCGCCCCAAACACAAGAAATACAAAAAACAAACCTGAAAAACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCt                                                                                                                <  1:507830/148‑1 (MQ=255)
                                      aGTAGCTAAAATCATCGCCGCAAACAGCAGAAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCt                                                                                                                <  1:1720852/150‑1 (MQ=255)
                                       gTAGCTAAAATCATCGCCGCAAACATCAGAAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCtt                                                                                                               <  1:1312221/150‑1 (MQ=255)
                                         aGCTAAAATCATCGCCGAAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTg                                                                                                             <  2:4324679/150‑1 (MQ=255)
                                         aGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTg                                                                                                             >  2:1691342/1‑150 (MQ=255)
                                         aGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTg                                                                                                             >  2:2221164/1‑150 (MQ=255)
                                         aGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCGATCGATCTCATCAAATATGGCTCGGtttt                                                                                                             >  2:4078485/1‑149 (MQ=255)
                                           cGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTgag                                                                                                           <  1:347969/150‑1 (MQ=255)
                                            gAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTATTTCAGTTGTGAAACTGAATCGATTTAGTCGCTATCGATCTCATAAAAAATTGCTCGCGTTgtga                                                                                                          >  1:1495449/1‑150 (MQ=255)
                                              aaaTCAACGCAGCAAACAGCAGCAATACATAACAGACACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGata                                                                                                        <  2:2638899/150‑1 (MQ=255)
                                               aaTCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGatat                                                                                                       <  2:4261077/150‑1 (MQ=255)
                                                    tcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTc                                                                                                  <  1:3310711/150‑1 (MQ=255)
                                                    tcGCCGCAAACAGCAGAAATACATAACACAAACCTGAAACACAAACCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTc                                                                                                  <  1:2188851/150‑1 (MQ=255)
                                                     cgccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCa                                                                                                 <  2:1256194/150‑1 (MQ=255)
                                                      gccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCaa                                                                                                <  2:1772275/150‑1 (MQ=255)
                                                        cgcAAACAGCAGCAATCCATAACAGAAACCTGAAACACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGt                                                                                              <  1:1775166/150‑1 (MQ=255)
                                                         gcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTATGTAAGTTGTTAAAATGAAACGATTTTTTCGCTATAGATCTCATCAAATATGGCTCGCTTTTTGATATTACTCAAGTa                                                                                             >  1:766375/1‑150 (MQ=255)
                                                          aaaccagcagCAATACATAACAGAACCCTCAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTaa                                                                                            <  1:4444019/146‑1 (MQ=255)
                                                           aaaCAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTATGTCAGTTGTGAAACGGAAGCGATTTAGTAGCAATCGATATCATCAAATATGGCTCGCTTTTTGATATTCCTCaatgtaa                                                                                           >  1:3488899/1‑145 (MQ=255)
                                                           aaaCAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTGTTTTTATTATGTCAGTTGTGTAACAGAATCGATTTATGAGCGATCGAGCTCCTCAAAAATGGCTCGCTTTGAGATAATCCGCAAGaaaa                                                                                           >  1:4190633/1‑150 (MQ=255)
                                                            aaCAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTGTTTTTATTCTGTCAGTTGTGGAACTGAAGCGATTTAGTCGCGATCGCTCTCATCAAATATGGCGCGCGTTGTGCTAATCCTCAAGTaaaa                                                                                          >  2:4427048/1‑150 (MQ=255)
                                                                 cagcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACAGAAGAGATTTAGTCGCAATCGATCACATCAAAAAAGGCTCGCTTTGAGATATTACTCAAGTaaaaaaaaa                                                                                     >  1:1825917/1‑148 (MQ=255)
                                                                     aaTACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTGTTTTTTTTCTGTAAGTTTTGTAACAGAATCGATTTAGTCGCGATCGATGTCATCAAATATTTCGAGCTTTGTGTTATTACTCAATTAAAAAAACaaatc                                                                                 >  2:1591099/1‑146 (MQ=255)
                                                                     aaTACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTGTTTTTATTCGGTCAGGTGTGAAACAGAAGCGATTTAGTCGCTCTCGATCTCAGCAAAAATGGCTCGCGTTGAGATATTCCACAACGAAAAAAACAactc                                                                                 >  2:2837333/1‑150 (MQ=255)
                                                                         cctAACAGAAACCTGAAACACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTcc                                                                             <  2:118425/148‑1 (MQ=255)
                                                                         cATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTTTTATGTCAGTTTTGGAACTGAAGCGATTTAGTCGCGATCGATCTCATCAAATATGGGTCGCTTTGAGATATTACTAAAGTAAAAAAACATATCTTcc                                                                             >  2:1347224/1‑150 (MQ=255)
                                                                          aTAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCt                                                                            <  2:3434677/150‑1 (MQ=255)
                                                                          aTAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCt                                                                            <  2:3434708/150‑1 (MQ=255)
                                                                             aCATAACCCTTAAACACCACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCg                                                                         <  1:3963172/150‑1 (MQ=255)
                                                                             aCAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCg                                                                         <  2:3413786/150‑1 (MQ=255)
                                                                             aCAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCg                                                                         <  2:709945/150‑1 (MQ=255)
                                                                                 aaaCCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTTTTCTGTCAGTTGTGTAACTGAAACGATTTAGTCGCTAACGGTCTCATCAAATATGGCTCGCTTTGAGTTATTCCTCAAGTAAAAAAACATCTCTTCCTGCGAttt                                                                     >  2:1523623/1‑150 (MQ=255)
                                                                                   aCCTGAAACACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTtct                                                                   <  1:3067856/150‑1 (MQ=255)
                                                                                    ccTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTTTTATTTCCGTTGTGAAACAGAAACGATTTTTTCGCTATCGTTCTCATCAAATATGGGTCGCTTTGAGATAGTACGCAAGTAAAAAAAAATCTAGTACTGCGAtttata                                                                  >  2:3628684/1‑147 (MQ=255)
                                                                                      tGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGTTCTCATCAAATATGGCTCGCTTTGAGATATTCCGCAAGTAAAAAAAAATCTCTTGCGGCGCTGTCTcac                                                                >  2:3079281/1‑150 (MQ=255)
                                                                                                aaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAtt                                                      <  1:1162396/150‑1 (MQ=255)
                                                                                                aaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAtt                                                      <  1:1162384/150‑1 (MQ=255)
                                                                                                aaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAtt                                                      <  2:1947715/150‑1 (MQ=255)
                                                                                                     cAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTg                                                 <  1:2275938/150‑1 (MQ=255)
                                                                                                        cccTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACa                                              <  2:3842370/150‑1 (MQ=255)
                                                                                                               gCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTg                                       <  2:3310976/150‑1 (MQ=255)
                                                                                                                cTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGa                                      >  2:241581/1‑150 (MQ=255)
                                                                                                                    cGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACaaa                                  >  1:720394/1‑150 (MQ=255)
                                                                                                                      tttttttATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAAt                                <  2:1865547/150‑1 (MQ=255)
                                                                                                                      tttttttATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAAt                                <  1:1253046/150‑1 (MQ=255)
                                                                                                                      tttttttATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAAt                                <  1:1254677/150‑1 (MQ=255)
                                                                                                                       ttttttATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAAtt                               <  2:4024995/150‑1 (MQ=255)
                                                                                                                        tttttATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTa                              <  1:2047771/150‑1 (MQ=255)
                                                                                                                          tttATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATg                            <  1:2066790/150‑1 (MQ=255)
                                                                                                                          tttATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATg                            >  1:4044762/1‑150 (MQ=255)
                                                                                                                          tttATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATg                            <  1:1549838/150‑1 (MQ=255)
                                                                                                                           ttATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATga                           <  1:611792/150‑1 (MQ=255)
                                                                                                                              ttCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGAt                        <  1:108363/150‑1 (MQ=255)
                                                                                                                              ttCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGAt                        <  1:108366/150‑1 (MQ=255)
                                                                                                                                 tGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGAtttt                     <  1:3843100/150‑1 (MQ=255)
                                                                                                                                 tGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGAtttt                     <  1:1400102/150‑1 (MQ=255)
                                                                                                                                 tGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGAtttt                     <  1:1400272/150‑1 (MQ=255)
                                                                                                                                 tGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGAtttt                     <  1:3842079/150‑1 (MQ=255)
                                                                                                                                  gTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTtc                    <  1:607127/150‑1 (MQ=255)
                                                                                                                                    cAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTtcat                  >  1:2165810/1‑150 (MQ=255)
                                                                                                                                    cAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTtcat                  <  1:2814026/150‑1 (MQ=255)
                                                                                                                                     aGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTtcatc                 <  2:1259577/150‑1 (MQ=255)
                                                                                                                                          tgAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATcacaca            <  2:3231624/150‑1 (MQ=255)
                                                                                                                                          tgAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATcacaca            >  1:3106269/1‑150 (MQ=255)
                                                                                                                                           gAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACAt           >  2:4552541/1‑150 (MQ=255)
                                                                                                                                            aaaCTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACAtt          <  1:3107207/150‑1 (MQ=255)
                                                                                                                                            aaaCTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACAtt          <  1:4563489/150‑1 (MQ=255)
                                                                                                                                              aCTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACAtttt        <  1:4718475/150‑1 (MQ=255)
                                                                                                                                              aCTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACAtttt        <  1:3228962/150‑1 (MQ=255)
                                                                                                                                              aCTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACAtttt        <  1:4718280/150‑1 (MQ=255)
                                                                                                                                                   aGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACATTTTGACAt   <  2:524649/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACATTTTGACATC  >  CP009273/2730165‑2730462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A