Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,730,313 G→A 21.6% intergenic (+71/‑200) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,730,3130GA21.6% 452.3 / 124.5 231intergenic (+71/‑200)bamD/raiABamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
Reads supporting (aligned to +/- strand):  ref base G (70/111);  new base A (26/24);  total (96/135)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

Only 100 of 231 total aligned reads displayed.

CATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACATTTTGACAT  >  CP009273/2730166‑2730461
                                                                                                                                                   |                                                                                                                                                    
cATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCg                                                                                                                                                    <  1:4736881/150‑1 (MQ=255)
cATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCg                                                                                                                                                    >  2:4925446/1‑150 (MQ=255)
 atcccGTCATATGCAGATGAATGCGCAAGCTTAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGa                                                                                                                                                   <  1:2396622/147‑1 (MQ=255)
   aCCGTCAGAGGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGAtt                                                                                                                                                 <  2:4504985/150‑1 (MQ=255)
    ccGTCAGATGCAGATGACTGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAttt                                                                                                                                                <  2:73200/150‑1 (MQ=255)
    ccGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAttt                                                                                                                                                <  2:3893761/150‑1 (MQ=255)
     cGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTATGTCAGTTGTGAAACAGAAGCGAGTTa                                                                                                                                               >  1:4882594/1‑150 (MQ=255)
     cGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAAACATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTa                                                                                                                                               <  1:264900/150‑1 (MQ=255)
      gTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAg                                                                                                                                              >  2:3339033/1‑150 (MQ=255)
       tCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGt                                                                                                                                             <  1:2612143/150‑1 (MQ=255)
         agatgcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACACAACAGAAACCTGAAACACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCg                                                                                                                                           <  2:2194402/150‑1 (MQ=255)
          gatgcagatgAATGCGCACGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGc                                                                                                                                          <  2:110445/150‑1 (MQ=255)
          gatgcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGGAACTGAAGCGATTTTGTCGc                                                                                                                                          >  1:2149074/1‑150 (MQ=255)
           atgcagatgAATGCGCACGCTGAAAAAGTAGCTAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCt                                                                                                                                         <  1:1096171/150‑1 (MQ=255)
           atgcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTAAGTTGTGTAACTGAAGCGATTTAGTCGCt                                                                                                                                         >  1:3575252/1‑150 (MQ=255)
              cagatgAATGCGCACGCTGAAAAAGTAGCGAAAATCATCGCCGCAACCAGCAGCAATACATAACAGAAACCTGAAACCCAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATc                                                                                                                                      <  1:2365997/150‑1 (MQ=255)
              cagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACCGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTANc                                                                                                                                      <  1:3714410/150‑1 (MQ=255)
              cagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTATGTCAGTTGTGAAACTTAAGCGATTTAGTCGCTATc                                                                                                                                      >  2:2314280/1‑150 (MQ=255)
               agatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATcc                                                                                                                                     >  1:3311919/1‑149 (MQ=255)
                 atgAATGCGCACGCTGAAAAAGTAGCGAACATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAt                                                                                                                                   <  2:669772/150‑1 (MQ=255)
                 atgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATACCAGAAACCTGAATCACAAAACGGCCGCCCTTGAGCTGCCGTATTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAt                                                                                                                                   <  2:4831439/150‑1 (MQ=255)
                 atgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCCGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAt                                                                                                                                   <  2:3001764/150‑1 (MQ=255)
                         gcAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGTAAAGATTTAGTCGCTATAGATCTCATCaa                                                                                                                           >  2:1736660/1‑150 (MQ=255)
                             gCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATCCATAACACAAACCTGAAACACAAACCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAatat                                                                                                                       <  1:4429132/150‑1 (MQ=255)
                             gCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGACACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAatat                                                                                                                       <  2:2659798/150‑1 (MQ=255)
                               ttaaaaaGTAGCGAAAATCATCGCCGCAAACAGCAGAAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATgg                                                                                                                     <  1:4238361/148‑1 (MQ=255)
                                    aaGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGc                                                                                                                >  2:5104493/1‑150 (MQ=255)
                                     aGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGGTCTCATCAAATATGGCTCGCt                                                                                                               >  2:1550997/1‑150 (MQ=255)
                                         gCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAACCCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTga                                                                                                           <  2:4076416/150‑1 (MQ=255)
                                         gCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACCCAAACCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTga                                                                                                           <  2:1831906/150‑1 (MQ=255)
                                          cGAAAATCATCGCCGCAAACAGCAGCAATACAGAACAGAAACCTGAAACACACAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTgag                                                                                                          <  2:4018689/150‑1 (MQ=255)
                                            aaaaTCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATAAAATATGGCTCGCTTTgagtt                                                                                                        >  2:255550/1‑148 (MQ=255)
                                            aaaaTCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTGTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATAAAATATGGCTCGCGTTTAGat                                                                                                        >  2:5084657/1‑150 (MQ=255)
                                              aaTCATCGCCGCAAACAGCAGCAATACATAACAGCAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGatat                                                                                                      <  1:2853091/150‑1 (MQ=255)
                                              aaTCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGAGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGttat                                                                                                      >  2:3497489/1‑150 (MQ=255)
                                               atcatcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCGTTGAGATAtt                                                                                                     >  2:806863/1‑150 (MQ=255)
                                               atcatcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACAGAAGCGAGTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATAtt                                                                                                     >  2:806915/1‑150 (MQ=255)
                                                 catcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTcc                                                                                                   <  1:3821646/150‑1 (MQ=255)
                                                  atcGCCGCAAACAGCAGCAATACATAACCGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCt                                                                                                  <  1:1417557/150‑1 (MQ=255)
                                                  atcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACACACCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCt                                                                                                  <  2:1021352/150‑1 (MQ=255)
                                                  atcGCCGCAAACAGCAGAAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCt                                                                                                  <  1:2459866/150‑1 (MQ=255)
                                                  atcGCCGAAAACAGCAGCAATACATAACAGAAACCTGAAACACACAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCt                                                                                                  <  1:5039993/150‑1 (MQ=255)
                                                      ccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAg                                                                                              <  1:5106486/150‑1 (MQ=255)
                                                         cAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTaa                                                                                           <  2:3657749/150‑1 (MQ=255)
                                                           aaCAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACGGAAGCGATTTAGTCGCTATCGCTCTCATCAAATATGGCTCGCGTTGAGATATTCCTCAAGTaaaa                                                                                         >  2:1487947/1‑150 (MQ=255)
                                                            aCAGCAGCAATACATAACAGAAACCTGAAACACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTaaaaa                                                                                        <  2:4481526/150‑1 (MQ=255)
                                                             cagcagCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTaaaaaa                                                                                       >  2:5131738/1‑150 (MQ=255)
                                                                  gcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTGTGTCAGTTGTTTAACAGAAGCGATTTATTAGCTATCGATCTCATCAAATATTGCGCGCTTTGTGATATTGCACAAGTaaaaaaaaaaa                                                                                  >  1:3164108/1‑146 (MQ=255)
                                                                  gcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTATGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACaac                                                                                  >  2:2679530/1‑148 (MQ=255)
                                                                    aaTACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTTTTCTGTCAGTTTTGAAACTGAAGCGATTTAGTCGCTATAGGTCTCATCAAATTTGGCTCGCGTTGGGATATTACTCAAGTaaaaaaaaaacta                                                                                >  1:1229277/1‑144 (MQ=255)
                                                                    aaTACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGTAACTGAAGCGATTTATTCGCTATAGATCTCAGAAAAAATGGCTCGCTTTGAGATATTACTCAAGGAAAAAAAAAtctc                                                                                >  1:3664296/1‑150 (MQ=255)
                                                                       aCATAACAGAAACCTGAAACACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTc                                                                             <  2:434801/150‑1 (MQ=255)
                                                                         aTAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCt                                                                           <  1:2106113/150‑1 (MQ=255)
                                                                         aTAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCt                                                                           <  1:3036332/150‑1 (MQ=255)
                                                                            aCATAAACCTGAAACCCAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCg                                                                        <  2:1570823/150‑1 (MQ=255)
                                                                              aGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGAt                                                                      <  1:2619104/150‑1 (MQ=255)
                                                                                accccTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGAttt                                                                    <  2:1278565/147‑1 (MQ=255)
                                                                                  aCCTGAAACACAACACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTtct                                                                  <  1:3068514/150‑1 (MQ=255)
                                                                                  aCCTGAAACACAAACCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTtct                                                                  <  2:3181038/150‑1 (MQ=255)
                                                                                   ccTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTtctc                                                                 <  2:4527762/150‑1 (MQ=255)
                                                                                     tGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTTTTATGTCAGTTGTGAAACTGAAACGATTTAGGCGCGATCGATCTCATCAAATATGGCTCGCTTTGTGATATTCCTCAAGGAAAAAAAAATATATTCATGCGATTTATcac                                                               >  2:4901486/1‑150 (MQ=255)
                                                                                      gAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTcaca                                                              <  1:4016274/150‑1 (MQ=255)
                                                                                      gAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTcaca                                                              <  1:4217121/150‑1 (MQ=255)
                                                                                              acaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAt                                                      <  1:590010/148‑1 (MQ=255)
                                                                                              aaaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAt                                                      <  2:4240821/150‑1 (MQ=255)
                                                                                              aaaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAt                                                      <  2:707512/150‑1 (MQ=255)
                                                                                               aaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGCAACTGAAACGTTTTAGTCGGTATCGGTCTCATCAAATATGGCGCGCTGTGTGATATTCCGCAAGTTAAAAAACATATCGTCCGGCGATTTCTCACAAAAAAGAtt                                                     >  2:4893275/1‑150 (MQ=255)
                                                                                               aaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAtt                                                     <  1:127347/150‑1 (MQ=255)
                                                                                               aaCGGCAGCCCTTGAGCTGCAGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAtt                                                     <  2:3119309/150‑1 (MQ=255)
                                                                                                ccGGCAGCCCTTGAGCTGCCTTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTc                                                    <  1:45428/149‑1 (MQ=255)
                                                                                                aCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTc                                                    <  2:1670116/150‑1 (MQ=255)
                                                                                                aCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTc                                                    <  2:2372275/150‑1 (MQ=255)
                                                                                                 cGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATTGCTCGCTTTGAGATATTCCTCAAGTAAAAAAAAATCTCTTCCTGCGATTTCTCACAAAAAAGATTCg                                                   >  2:4744270/1‑150 (MQ=255)
                                                                                                  ggCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTATCACAAAAAAGATTCGt                                                  >  2:1303027/1‑150 (MQ=255)
                                                                                                   gCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGtt                                                 <  1:4316117/150‑1 (MQ=255)
                                                                                                   gCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGtt                                                 <  1:212487/150‑1 (MQ=255)
                                                                                                     aGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGa                                               <  1:1854876/150‑1 (MQ=255)
                                                                                                       cccTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACa                                             <  2:487267/150‑1 (MQ=255)
                                                                                                         cTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACaaa                                           <  1:5070408/150‑1 (MQ=255)
                                                                                                           tGAGCTGCCGTTTTTTTATTATGTCACTTTTGAAACTGAAACGATTTATTCGCTATCGATAACATCAAAAATTGCTCGCTTTGAGATATTACTCAAATAAAAAAACAACTCTTCCAGCGATTTATAACAAAAAAAATTAGTTTAaaaaaa                                         >  1:3443688/1‑150 (MQ=255)
                                                                                                             aGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGt                                       <  2:3311919/150‑1 (MQ=255)
                                                                                                                tGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATAGATCTCATCAAATATGGCTCGCTTTTAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTTACAAAAAGTGAc                                    >  2:506760/1‑150 (MQ=255)
                                                                                                                 gCCGTATTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACa                                   <  2:3380149/150‑1 (MQ=255)
                                                                                                                    gTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACaaaa                                >  1:1939535/1‑150 (MQ=255)
                                                                                                                       ttattATTCTGTCAGTTGTGAAACTGAAGCGAGTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATGTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTa                             <  2:3554102/147‑1 (MQ=255)
                                                                                                                        ttttATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTAt                            <  1:703252/150‑1 (MQ=255)
                                                                                                                        ttttATTCTGTCAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTAt                            <  1:1770277/150‑1 (MQ=255)
                                                                                                                         tttATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATg                           <  1:3339033/150‑1 (MQ=255)
                                                                                                                         tttATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATg                           >  1:2596333/1‑150 (MQ=255)
                                                                                                                          ttATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATga                          >  1:4663045/1‑150 (MQ=255)
                                                                                                                           tATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATAGTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATgag                         <  1:2820136/150‑1 (MQ=255)
                                                                                                                              tCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGAtt                      <  2:1347029/150‑1 (MQ=255)
                                                                                                                                   cAGTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTtcat                 >  1:617453/1‑150 (MQ=255)
                                                                                                                                     gTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTtcatca               <  2:517295/150‑1 (MQ=255)
                                                                                                                                     gTTGTGAAACTGAAACGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTtcatca               <  2:345432/150‑1 (MQ=255)
                                                                                                                                             aCTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACAtttt       >  2:1142888/1‑150 (MQ=255)
                                                                                                                                               tGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACAATTTGa     >  1:2471391/1‑150 (MQ=255)
                                                                                                                                                gAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACATTTTGAc    <  2:1939455/150‑1 (MQ=255)
                                                                                                                                                 aaGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACATGTTGACa   >  1:5050479/1‑150 (MQ=255)
                                                                                                                                                  aGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACATTTTGACAt  >  2:5031421/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAAAGTGACAAAATTATGAGATTTTCATCACACATTTTGACAT  >  CP009273/2730166‑2730461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A