Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,504,362 A→G 7.1% intergenic (‑27/+57) yhfX ← / ← yhfY putative pyridoxal 5'‑phosphate binding protein/PRD domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,504,3620AG 7.1% 743.1 / 35.0 224intergenic (‑27/+57)yhfX/yhfYputative pyridoxal 5'‑phosphate binding protein/PRD domain protein
Reads supporting (aligned to +/- strand):  ref base A (91/117);  new base G (10/6);  total (101/123)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 228 total aligned reads displayed.

GTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCTT  >  CP009273/3504213‑3504511
                                                                                                                                                     |                                                                                                                                                     
gTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGaa                                                                                                                                                       <  1:3193393/150‑1 (MQ=255)
 ttttCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAAt                                                                                                                                                      <  1:3939523/150‑1 (MQ=255)
  tttCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAAtt                                                                                                                                                     <  2:2692919/150‑1 (MQ=255)
     ccAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTaaa                                                                                                                                                  >  1:2843580/1‑150 (MQ=255)
      cAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAat                                                                                                                                                 <  1:1337581/150‑1 (MQ=255)
         tATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATAtt                                                                                                                                              >  2:3417360/1‑150 (MQ=255)
          aTCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTc                                                                                                                                             <  2:1628513/150‑1 (MQ=255)
            cTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCAt                                                                                                                                           >  1:2738308/1‑150 (MQ=255)
             tGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCAtt                                                                                                                                          >  1:4127640/1‑150 (MQ=255)
              gATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCAttt                                                                                                                                         <  1:4644871/150‑1 (MQ=255)
                 ccACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCAtttttt                                                                                                                                      <  2:4074016/150‑1 (MQ=255)
                   aCGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTg                                                                                                                                    <  2:2065268/150‑1 (MQ=255)
                   aCGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTg                                                                                                                                    <  1:2899529/150‑1 (MQ=255)
                   aCGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTg                                                                                                                                    <  2:3908499/150‑1 (MQ=255)
                    cGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGa                                                                                                                                   <  2:4702864/150‑1 (MQ=255)
                       cGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAata                                                                                                                                <  2:3284146/150‑1 (MQ=255)
                       cGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAata                                                                                                                                >  2:2637362/1‑150 (MQ=255)
                        gATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGCCGGAAAACCGCGCCGGGTGATGGCGTTTCATTCCTGCAACAAACACCTGGTTGCAGCGTTGCTGTGCCTTGAGTTAAATATCCATTTTTTGTAatat                                                                                                                               >  2:4313442/1‑150 (MQ=255)
                        gATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCAGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACCTCTTGTTGCCTCGTTTCTGTTCCTTGAAATAAATATTCATTTTTGTGAatat                                                                                                                               >  1:1056485/1‑150 (MQ=255)
                         aTCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATAtt                                                                                                                              <  1:69518/150‑1 (MQ=255)
                          tCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATAttt                                                                                                                             >  1:1322882/1‑150 (MQ=255)
                            aCCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAg                                                                                                                           <  2:2738279/150‑1 (MQ=255)
                            aCCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAg                                                                                                                           >  1:4559079/1‑150 (MQ=255)
                             cccAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCATCGCCGGGTTCTTGCTTTTCAATGCTTCTAAAAACATCTTGTTGCCTCGTTTCTGTTCATTGAATTAAATATTCTTTTTTTTGAATATTTAgg                                                                                                                          <  1:1804305/150‑1 (MQ=255)
                               cAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGtt                                                                                                                        >  2:2910670/1‑150 (MQ=255)
                               cAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGtt                                                                                                                        >  2:2453228/1‑150 (MQ=255)
                                aGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTtg                                                                                                                       >  1:1675017/1‑150 (MQ=255)
                                aGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTtg                                                                                                                       <  1:1509007/150‑1 (MQ=255)
                                aGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTtg                                                                                                                       <  2:2279801/150‑1 (MQ=255)
                                 gCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTtgt                                                                                                                      <  1:2127161/150‑1 (MQ=255)
                                  cTGTCCGGGGCGATCTTGCCCTTCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTtgtg                                                                                                                     <  1:776444/150‑1 (MQ=255)
                                   tGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGc                                                                                                                    <  1:2680874/150‑1 (MQ=255)
                                    gTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCg                                                                                                                   <  2:4742465/150‑1 (MQ=255)
                                     tCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGGGCGGCGGAAATCAGCGCAGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGa                                                                                                                  >  2:2219250/1‑150 (MQ=255)
                                       cGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACg                                                                                                                <  1:2808681/150‑1 (MQ=255)
                                          ggCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCGTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTgg                                                                                                             <  2:3988520/150‑1 (MQ=255)
                                            cGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGAt                                                                                                           <  1:4381259/150‑1 (MQ=255)
                                             gATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTCCAAACCTCTTGTTGCCTCGTGTCTGTTCCGTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGAtt                                                                                                          <  2:2477582/150‑1 (MQ=255)
                                              aTCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGAttt                                                                                                         >  1:2409713/1‑150 (MQ=255)
                                                  tGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCg                                                                                                     >  1:3427845/1‑150 (MQ=255)
                                                   gCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCgg                                                                                                    <  1:1740073/150‑1 (MQ=255)
                                                   gCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCgg                                                                                                    >  2:2184809/1‑150 (MQ=255)
                                                   gCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCgg                                                                                                    <  2:2637855/150‑1 (MQ=255)
                                                   gCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCgg                                                                                                    <  2:2818551/150‑1 (MQ=255)
                                                         ctgcCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCtt                                                                                              <  2:3560006/150‑1 (MQ=255)
                                                           gcCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCa                                                                                            <  2:2268282/150‑1 (MQ=255)
                                                             cacaGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCaaa                                                                                          >  2:3823308/1‑150 (MQ=255)
                                                              acaGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAAc                                                                                         <  1:602713/150‑1 (MQ=255)
                                                                aGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACct                                                                                       <  1:1241005/150‑1 (MQ=255)
                                                                aGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACct                                                                                       >  1:1638940/1‑150 (MQ=255)
                                                                aGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACct                                                                                       <  1:31107/150‑1 (MQ=255)
                                                                   cTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGGTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCtt                                                                                    <  2:3334898/150‑1 (MQ=255)
                                                                    ttAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTc                                                                                   <  1:258784/150‑1 (MQ=255)
                                                                         gcggcgGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTag                                                                              <  2:3543051/150‑1 (MQ=255)
                                                                          cggcggAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTagc                                                                             >  1:4010320/1‑150 (MQ=255)
                                                                          cggcggAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTagc                                                                             >  1:4100054/1‑150 (MQ=255)
                                                                          cggcggAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTagc                                                                             >  1:2373364/1‑150 (MQ=255)
                                                                                  aTCAGCGCCGGGTTCTGGCGTTTCCATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGACTATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGccatcc                                                                     >  2:3997960/1‑150 (MQ=255)
                                                                                   tCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGccatcca                                                                    <  2:166717/150‑1 (MQ=255)
                                                                                   tCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTCAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGccatcca                                                                    <  2:2186457/150‑1 (MQ=255)
                                                                                    cAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTAATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGccatccat                                                                   >  1:484220/1‑150 (MQ=255)
                                                                                     aGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCAATTTTTTGAATATTTAGGTTGTTCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCatcccta                                                                  >  1:1539820/1‑150 (MQ=255)
                                                                                      gcgcCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCatccataa                                                                 >  1:3842103/1‑150 (MQ=255)
                                                                                       cgcCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCatccataat                                                                <  2:1259830/150‑1 (MQ=255)
                                                                                            ggTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCatccataatccata                                                           >  1:1258845/1‑150 (MQ=255)
                                                                                             gTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCAtccataatccataa                                                          <  1:1559862/150‑1 (MQ=255)
                                                                                             gTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCAtccataatccataa                                                          >  2:1488759/1‑150 (MQ=255)
                                                                                               tCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAg                                                        <  1:1069122/150‑1 (MQ=255)
                                                                                               tCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAg                                                        <  1:3754190/150‑1 (MQ=255)
                                                                                               tCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAg                                                        >  1:1148520/1‑150 (MQ=255)
                                                                                                 tGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGt                                                      <  2:4494663/150‑1 (MQ=255)
                                                                                                  ggCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGtt                                                     <  1:2297685/150‑1 (MQ=255)
                                                                                                   gCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTg                                                    <  2:1786939/150‑1 (MQ=255)
                                                                                                    cGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTgg                                                   <  2:3163282/150‑1 (MQ=255)
                                                                                                        tCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAg                                               >  1:4270732/1‑150 (MQ=255)
                                                                                                             gCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAAt                                          <  1:4615652/150‑1 (MQ=255)
                                                                                                              cTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATa                                         <  1:744671/150‑1 (MQ=255)
                                                                                                              cTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATa                                         <  2:1963752/150‑1 (MQ=255)
                                                                                                               ttCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAg                                        <  2:1177188/150‑1 (MQ=255)
                                                                                                                 cTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGcc                                      >  1:2876879/1‑150 (MQ=255)
                                                                                                                   aCAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCctt                                    >  2:4161743/1‑150 (MQ=255)
                                                                                                                      aaCATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCcttctt                                 <  2:686586/150‑1 (MQ=255)
                                                                                                                      aaCATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCcttctt                                 >  1:1819181/1‑150 (MQ=255)
                                                                                                                           cTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCacac                            >  2:608192/1‑150 (MQ=255)
                                                                                                                             tgttgCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACgg                          <  2:3242120/150‑1 (MQ=255)
                                                                                                                              gttgCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACggg                         <  1:2853505/150‑1 (MQ=255)
                                                                                                                                 gCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTa                      >  2:4512274/1‑150 (MQ=255)
                                                                                                                                    tCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACt                   <  1:3832376/150‑1 (MQ=255)
                                                                                                                                     cGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACtt                  <  1:73236/150‑1 (MQ=255)
                                                                                                                                        ttCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCa               <  1:3587499/150‑1 (MQ=255)
                                                                                                                                        ttCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCa               >  1:1266237/1‑150 (MQ=255)
                                                                                                                                        ttCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCa               >  2:1506921/1‑150 (MQ=255)
                                                                                                                                          cTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGt             <  1:2698284/150‑1 (MQ=255)
                                                                                                                                            gTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGc           <  2:4740453/150‑1 (MQ=255)
                                                                                                                                               ccTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGaa        <  1:1244801/150‑1 (MQ=255)
                                                                                                                                                cTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAAt       >  1:2711720/1‑150 (MQ=255)
                                                                                                                                                cTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAAt       >  2:1878627/1‑150 (MQ=255)
                                                                                                                                                   gAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTcc    >  2:722849/1‑150 (MQ=255)
                                                                                                                                                     aTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGACTTCCtt  >  2:2022736/1‑150 (MQ=255)
                                                                                                                                                     aTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCtt  >  1:1808662/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCTT  >  CP009273/3504213‑3504511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A