Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,504,362 A→G 25.8% intergenic (‑27/+57) yhfX ← / ← yhfY putative pyridoxal 5'‑phosphate binding protein/PRD domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,504,3620AG25.8% 436.7 / 181.0 236intergenic (‑27/+57)yhfX/yhfYputative pyridoxal 5'‑phosphate binding protein/PRD domain protein
Reads supporting (aligned to +/- strand):  ref base A (60/115);  new base G (35/26);  total (95/141)
Fisher's exact test for biased strand distribution p-value = 2.28e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

GTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCT  >  CP009273/3504213‑3504510
                                                                                                                                                     |                                                                                                                                                    
 ttttCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAAt                                                                                                                                                     <  1:3377792/150‑1 (MQ=255)
  tttCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAAtt                                                                                                                                                    <  2:1150932/150‑1 (MQ=255)
   ttCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTa                                                                                                                                                   <  2:2629352/150‑1 (MQ=255)
    tCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTaa                                                                                                                                                  <  2:1751693/150‑1 (MQ=255)
     ccAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTaaa                                                                                                                                                 >  1:4070551/1‑150 (MQ=255)
      cAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATATTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTACTACAAACATCTTGTTGCCACGTTTCTGTTGCTAGAGTTAAat                                                                                                                                                >  2:234153/1‑150 (MQ=255)
       aGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAata                                                                                                                                               >  1:63553/1‑150 (MQ=255)
        gTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAatat                                                                                                                                              <  2:2210556/150‑1 (MQ=255)
        gTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAatat                                                                                                                                              >  2:2554654/1‑150 (MQ=255)
         tATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATAtt                                                                                                                                             <  1:1781922/150‑1 (MQ=255)
          aTCTGATCCATGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTc                                                                                                                                            >  1:3460157/1‑150 (MQ=255)
            cTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCAt                                                                                                                                          <  1:1888760/150‑1 (MQ=255)
             tGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCAtt                                                                                                                                         <  1:3344088/150‑1 (MQ=255)
              gATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCAttt                                                                                                                                        >  1:561290/1‑150 (MQ=255)
              gATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCAttt                                                                                                                                        <  1:994386/150‑1 (MQ=255)
                tCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCAttttt                                                                                                                                      <  1:921400/150‑1 (MQ=255)
                 ccACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCAtttttt                                                                                                                                     <  1:2845691/150‑1 (MQ=255)
                  cACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTAAttttttt                                                                                                                                    >  1:1260054/1‑150 (MQ=255)
                  cACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTAAttttttt                                                                                                                                    >  1:1260630/1‑150 (MQ=255)
                  cACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCAttttttt                                                                                                                                    <  2:1514535/150‑1 (MQ=255)
                   aCGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTg                                                                                                                                   >  1:1034528/1‑150 (MQ=255)
                      tCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAat                                                                                                                                <  2:3977346/150‑1 (MQ=255)
                       cGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTAATTTTTTTGAata                                                                                                                               >  2:2483312/1‑150 (MQ=255)
                        gATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAatat                                                                                                                              >  2:1391404/1‑150 (MQ=255)
                         aTCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATAtt                                                                                                                             >  1:2699634/1‑150 (MQ=255)
                         aTCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATAtt                                                                                                                             <  2:807197/150‑1 (MQ=255)
                         aTCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATAtt                                                                                                                             <  1:2504932/150‑1 (MQ=255)
                         aTCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATAtt                                                                                                                             <  1:4820964/150‑1 (MQ=255)
                           cACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTa                                                                                                                           >  2:831676/1‑150 (MQ=255)
                           cACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTAAATGATTCTACAAAAATCTTGTTTCCTCGTTTCTTTTACTTTAATTAAATATTAATTTTTTTTAATATTTa                                                                                                                           >  1:1628845/1‑150 (MQ=255)
                            aCCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAg                                                                                                                          >  2:4666404/1‑150 (MQ=255)
                             cccAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAgg                                                                                                                         <  2:951373/150‑1 (MQ=255)
                             cccAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAgg                                                                                                                         <  1:3589705/150‑1 (MQ=255)
                               cAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGtt                                                                                                                       <  2:2257422/150‑1 (MQ=255)
                                 gCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTtgt                                                                                                                     >  2:3133676/1‑150 (MQ=255)
                                  cTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTtgtg                                                                                                                    <  2:3033257/150‑1 (MQ=255)
                                   tGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTATTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCATCTACAAAAATATTGTTGCCTCGTTTCTGTTCCTAGAGTTAAATATTCATTTTTTTGAATAATTAGGAtgtga                                                                                                                   >  1:793811/1‑149 (MQ=255)
                                        ggggCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGt                                                                                                              <  1:1703008/150‑1 (MQ=255)
                                        ggggCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGt                                                                                                              >  2:4427479/1‑150 (MQ=255)
                                          ggCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTgg                                                                                                            <  2:2333046/150‑1 (MQ=255)
                                              aTCTTGCCCTGCTGCCACAGGCTTCGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGAttt                                                                                                        <  2:1642076/150‑1 (MQ=255)
                                                    cccTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCggg                                                                                                  >  2:3652171/1‑150 (MQ=255)
                                                    cccTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCggg                                                                                                  <  2:4123364/150‑1 (MQ=255)
                                                         ctgcCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCtt                                                                                             <  1:2022514/150‑1 (MQ=255)
                                                          tgcCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTc                                                                                            <  1:900874/150‑1 (MQ=255)
                                                              acaGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGCTTTGTCGGGAGCTTCAAAc                                                                                        >  2:2638670/1‑150 (MQ=255)
                                                                aGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACct                                                                                      <  1:2146462/150‑1 (MQ=255)
                                                                  gCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACctct                                                                                    <  2:1888502/150‑1 (MQ=255)
                                                                  gCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACctct                                                                                    <  2:2239315/150‑1 (MQ=255)
                                                                    ttAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTc                                                                                  <  2:2056627/150‑1 (MQ=255)
                                                                    ttAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTc                                                                                  <  1:1900498/150‑1 (MQ=255)
                                                                     tAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCg                                                                                 >  1:4755088/1‑150 (MQ=255)
                                                                       gTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGtt                                                                               <  2:3187041/150‑1 (MQ=255)
                                                                           ggcggAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGTTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTagaa                                                                           >  2:2530458/1‑148 (MQ=255)
                                                                           ggcggAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTagca                                                                           <  1:1763695/150‑1 (MQ=255)
                                                                                aaaTCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGccat                                                                      <  2:1174195/150‑1 (MQ=255)
                                                                                 aaTCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGccatc                                                                     <  1:739986/150‑1 (MQ=255)
                                                                                 aaTCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGccatc                                                                     <  1:167570/150‑1 (MQ=255)
                                                                                   tCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGccatcca                                                                   >  2:48470/1‑150 (MQ=255)
                                                                                   tCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGccatcca                                                                   >  2:47322/1‑150 (MQ=255)
                                                                                     aGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCatccata                                                                 <  1:1404911/150‑1 (MQ=255)
                                                                                       cgcCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCatccataat                                                               <  1:1921060/150‑1 (MQ=255)
                                                                                          cGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCatccataatcca                                                            <  1:722858/150‑1 (MQ=255)
                                                                                          cGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCatccataatcca                                                            <  2:2859793/150‑1 (MQ=255)
                                                                                            ggTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCatccataatccata                                                          >  2:1617339/1‑150 (MQ=255)
                                                                                                cTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTAGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAgg                                                      >  2:1086349/1‑150 (MQ=255)
                                                                                                 tGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGt                                                     <  1:517100/150‑1 (MQ=255)
                                                                                                  ggCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGtt                                                    >  2:4343541/1‑150 (MQ=255)
                                                                                                   gCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTg                                                   <  2:4526354/150‑1 (MQ=255)
                                                                                                    cGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTgg                                                  <  2:63553/150‑1 (MQ=255)
                                                                                                      tttCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGcc                                                >  2:4104340/1‑150 (MQ=255)
                                                                                                       ttCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCa                                               <  2:4597171/150‑1 (MQ=255)
                                                                                                             gCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAAt                                         <  1:3012994/150‑1 (MQ=255)
                                                                                                              cTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCCTCCATAATCCATAAAGGTTGGCCAGCAAATa                                        >  1:3186124/1‑150 (MQ=255)
                                                                                                              cTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATa                                        >  2:1745875/1‑150 (MQ=255)
                                                                                                              cTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATa                                        >  2:4685196/1‑150 (MQ=255)
                                                                                                              cTTCTACAAACATATTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATa                                        <  1:4660236/150‑1 (MQ=255)
                                                                                                                tCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGc                                      >  1:1071186/1‑150 (MQ=255)
                                                                                                                         aTCTTGTTGCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTcac                             >  1:3150004/1‑150 (MQ=255)
                                                                                                                         aTCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTcac                             <  1:2466315/150‑1 (MQ=255)
                                                                                                                          tCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTcaca                            >  2:3882494/1‑150 (MQ=255)
                                                                                                                           cTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCacac                           >  1:3825090/1‑150 (MQ=255)
                                                                                                                            ttggtgCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACg                          <  1:1224430/150‑1 (MQ=255)
                                                                                                                             tgttgCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACgg                         >  1:3621024/1‑150 (MQ=255)
                                                                                                                                tgCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGtt                      <  1:414124/150‑1 (MQ=255)
                                                                                                                                 gCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTTTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATACATAATCCAAAAAGGTTGTCCAGCAAATAGCCATCTTCAAACGGGTTa                     >  1:3942297/1‑150 (MQ=255)
                                                                                                                                 gCCTCGTTTCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTa                     <  2:4219161/150‑1 (MQ=255)
                                                                                                                                 gCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTa                     <  2:3425776/150‑1 (MQ=255)
                                                                                                                                       tttCTGTTCCTTGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTcc               >  1:4375227/1‑150 (MQ=255)
                                                                                                                                             ttCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCg         <  2:1336835/150‑1 (MQ=255)
                                                                                                                                              tCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGa        >  2:2575798/1‑150 (MQ=255)
                                                                                                                                              tCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGa        <  1:110332/150‑1 (MQ=255)
                                                                                                                                               ccTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGaa       <  1:1491785/150‑1 (MQ=255)
                                                                                                                                               ccTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGaa       >  1:4444112/1‑150 (MQ=255)
                                                                                                                                               ccTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGaa       >  1:4388192/1‑150 (MQ=255)
                                                                                                                                                  tGAGTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTc    <  1:4218321/150‑1 (MQ=255)
                                                                                                                                                   gAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTcc   <  1:1636277/150‑1 (MQ=255)
                                                                                                                                                    aaTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCt  >  2:2338698/1‑150 (MQ=255)
                                                                                                                                                    aaTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCt  >  2:402012/1‑150 (MQ=255)
                                                                                                                                                    aaTTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCt  >  2:2797165/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCGGAAATCAGCGCCGGGTTCTGGCGTTTCAATGCTTCTACAAACATCTTGTTGCCTCGTTTCTGTTCCTTGAATTAAATATTCATTTTTTTGAATATTTAGGTTGTGCGACGTGGATTTGTCGGGAGCTTCAAACCTCTTCGTTAGCAGCCATCCATAATCCATAAAGGTTGGCCAGCAAATAGCCTTCTTCACACGGGTTAACTTCCAGTGCGAATTCCT  >  CP009273/3504213‑3504510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A