Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F0 I0 R1 81 326.4 10500568 93.1% 9776028 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,297,846 C→A 14.1% M150I (ATG→ATT)  alsE ← allulose‑6‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,297,8460CA14.1% 1289.0 / 169.5 389M150I (ATG→ATT) alsEallulose‑6‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (159/175);  new base A (28/27);  total (187/202)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 393 total aligned reads displayed.

AACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTC  >  CP009273/4297697‑4297995
                                                                                                                                                     |                                                                                                                                                     
aaCAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGc                                                                                                                                                       >  2:1747899/1‑150 (MQ=255)
  cAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAt                                                                                                                                                     <  1:1010327/150‑1 (MQ=255)
   aGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAtt                                                                                                                                                    <  1:4355729/150‑1 (MQ=255)
   aGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAtt                                                                                                                                                    >  2:2205525/1‑150 (MQ=255)
   aGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAtt                                                                                                                                                    >  2:454614/1‑150 (MQ=255)
    ggCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAttt                                                                                                                                                   >  2:870547/1‑150 (MQ=255)
    ggCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAttt                                                                                                                                                   <  2:1229119/150‑1 (MQ=255)
    ggCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAttt                                                                                                                                                   >  1:454185/1‑150 (MQ=255)
       cGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAg                                                                                                                                                >  1:2249211/1‑150 (MQ=255)
        ggAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAgg                                                                                                                                               >  2:1841131/1‑150 (MQ=255)
        ggAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAgg                                                                                                                                               >  2:5222529/1‑150 (MQ=255)
         gAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGa                                                                                                                                              >  2:1007963/1‑150 (MQ=255)
             tGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGa                                                                                                                                          >  1:535108/1‑150 (MQ=255)
              gCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGaa                                                                                                                                         <  1:4475528/150‑1 (MQ=255)
               ccGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAAc                                                                                                                                        <  2:1698671/150‑1 (MQ=255)
                 gacgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACgg                                                                                                                                      <  2:1303622/150‑1 (MQ=255)
                   cgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGtt                                                                                                                                    <  2:5043682/150‑1 (MQ=255)
                   cgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGtt                                                                                                                                    <  1:2094152/150‑1 (MQ=255)
                    gaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTg                                                                                                                                   <  1:1076667/150‑1 (MQ=255)
                    gaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTg                                                                                                                                   >  1:3499247/1‑150 (MQ=255)
                    gaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTg                                                                                                                                   <  2:1353819/150‑1 (MQ=255)
                      tAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTc                                                                                                                                 <  1:3751938/150‑1 (MQ=255)
                       aaaGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTcc                                                                                                                                >  1:3224788/1‑150 (MQ=255)
                         aGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACT‑CAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGc                                                                                                                             >  2:2756244/1‑150 (MQ=255)
                               ccGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGc                                                                                                                        >  1:4540031/1‑150 (MQ=255)
                                   cccTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCggg                                                                                                                    <  1:4259171/150‑1 (MQ=255)
                                      tGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATc                                                                                                                 <  1:1641270/150‑1 (MQ=255)
                                       gccgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCg                                                                                                                <  1:1267401/150‑1 (MQ=255)
                                        ccgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTCATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGa                                                                                                               <  2:320434/150‑1 (MQ=255)
                                             aTCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTc                                                                                                          >  1:2521673/1‑150 (MQ=255)
                                                aGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATg                                                                                                       >  1:4218934/1‑150 (MQ=255)
                                                 gTTTTTCGTAAGTTGCCTGGTGGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCACTTGTATCCAGCATTTCATGAATGAACGTTTGTCCGGAAAAGCCGGGATCGAAAGTCATGa                                                                                                      >  2:1090856/1‑150 (MQ=255)
                                                  tttttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGAc                                                                                                     >  1:4762849/1‑150 (MQ=255)
                                                     ttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGt                                                                                                  <  2:4507870/150‑1 (MQ=255)
                                                        gTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAt                                                                                               <  1:2910369/150‑1 (MQ=255)
                                                         tAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtt                                                                                              <  1:2303954/150‑1 (MQ=255)
                                                             ttGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTAt                                                                                          <  1:4646753/150‑1 (MQ=255)
                                                                   ggTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCt                                                                                    >  1:3514714/1‑150 (MQ=255)
                                                                         aGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGa                                                                              <  2:4486596/150‑1 (MQ=255)
                                                                          ggAACCGTCCACCTCAATTTCTTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGAAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGat                                                                             <  1:3653384/150‑1 (MQ=255)
                                                                          ggAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGat                                                                             <  1:3157016/150‑1 (MQ=255)
                                                                           gAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGata                                                                            >  1:1680994/1‑150 (MQ=255)
                                                                           gAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGata                                                                            <  1:3331192/150‑1 (MQ=255)
                                                                            aaCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGatat                                                                           >  2:3266421/1‑150 (MQ=255)
                                                                              ccGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAg                                                                         <  2:3447941/150‑1 (MQ=255)
                                                                              ccGTCCACCACAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAg                                                                         <  2:308307/150‑1 (MQ=255)
                                                                                gTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTa                                                                       >  2:5046889/1‑150 (MQ=255)
                                                                                 tCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAt                                                                      <  2:4820341/150‑1 (MQ=255)
                                                                                  ccACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAtt                                                                     >  1:2172855/1‑150 (MQ=255)
                                                                                   cACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAttt                                                                    >  2:1639338/1‑150 (MQ=255)
                                                                                       tCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATg                                                                <  1:868670/150‑1 (MQ=255)
                                                                                        cAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATgg                                                               >  2:3587955/1‑150 (MQ=255)
                                                                                         aaTTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGc                                                              >  1:4039011/1‑150 (MQ=255)
                                                                                          aTTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGcc                                                             <  2:3352720/150‑1 (MQ=255)
                                                                                          aTTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGcc                                                             >  1:2708795/1‑150 (MQ=255)
                                                                                            ttCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTc                                                           >  2:3107626/1‑150 (MQ=255)
                                                                                             tCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCa                                                          <  2:4970183/150‑1 (MQ=255)
                                                                                             tCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCa                                                          <  1:2807513/150‑1 (MQ=255)
                                                                                             tCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCa                                                          <  1:1116460/150‑1 (MQ=255)
                                                                                              cGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCaa                                                         >  1:2291968/1‑150 (MQ=255)
                                                                                                  cTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTgg                                                     <  2:4034704/150‑1 (MQ=255)
                                                                                                   tCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGc                                                    >  1:1348788/1‑150 (MQ=255)
                                                                                                    ccAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCg                                                   <  2:3707685/150‑1 (MQ=255)
                                                                                                     cAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGt                                                  >  1:2993234/1‑150 (MQ=255)
                                                                                                      aGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGtc                                                 <  2:1962550/150‑1 (MQ=255)
                                                                                                      aGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGtc                                                 <  2:679905/150‑1 (MQ=255)
                                                                                                          cTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCg                                             >  2:3323004/1‑150 (MQ=255)
                                                                                                          cTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCg                                             >  1:238297/1‑150 (MQ=255)
                                                                                                          cTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCg                                             >  1:3968093/1‑150 (MQ=255)
                                                                                                               cGTTCACGCCATGCCTTCAGTTCGGCTAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTa                                        <  2:4733435/150‑1 (MQ=255)
                                                                                                               cGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTa                                        <  2:535108/150‑1 (MQ=255)
                                                                                                                gTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTaa                                       <  2:4943096/150‑1 (MQ=255)
                                                                                                                gTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGAAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTaa                                       <  2:2576423/150‑1 (MQ=255)
                                                                                                                 ttCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAg                                      <  1:849502/150‑1 (MQ=255)
                                                                                                                  tCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAgg                                     >  2:2326308/1‑150 (MQ=255)
                                                                                                                   cACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCCCCGGGTTAAGGa                                    <  1:1754254/150‑1 (MQ=255)
                                                                                                                    aCGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGTATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGAt                                   <  1:2756244/150‑1 (MQ=255)
                                                                                                                      gCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCa                                 >  2:714029/1‑150 (MQ=255)
                                                                                                                            ccTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCa                           <  1:1870484/150‑1 (MQ=255)
                                                                                                                            ccTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCa                           <  2:287630/150‑1 (MQ=255)
                                                                                                                            ccTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCa                           >  1:721325/1‑150 (MQ=255)
                                                                                                                            ccTTCAGTTCGGCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCa                           >  1:372142/1‑150 (MQ=255)
                                                                                                                             cTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCAc                          >  1:969211/1‑150 (MQ=255)
                                                                                                                              ttCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGcccctca                         >  2:435137/1‑147 (MQ=255)
                                                                                                                                cAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACttt                       <  2:1172629/150‑1 (MQ=255)
                                                                                                                                 aGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtc                      >  2:3873520/1‑150 (MQ=255)
                                                                                                                                 aGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtc                      >  2:4207051/1‑150 (MQ=255)
                                                                                                                                   ttCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcat                    <  1:5102492/150‑1 (MQ=255)
                                                                                                                                   ttCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcat                    <  1:2476023/150‑1 (MQ=255)
                                                                                                                                   ttCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcat                    <  1:1494813/150‑1 (MQ=255)
                                                                                                                                      ggCAAGTTTATCCAGAATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtc                 >  1:922135/1‑150 (MQ=255)
                                                                                                                                        cAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtcat               >  2:2304694/1‑150 (MQ=255)
                                                                                                                                         aaGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGAACGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTGAAGGATCAGCCCCACTTtcatgtcatg              >  2:3780527/1‑150 (MQ=255)
                                                                                                                                          aGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGa             >  1:4944740/1‑150 (MQ=255)
                                                                                                                                                  cAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAt     >  2:3030590/1‑150 (MQ=255)
                                                                                                                                                  cAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAt     >  1:3841702/1‑150 (MQ=255)
                                                                                                                                                  cAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAt     <  2:3500375/150‑1 (MQ=255)
                                                                                                                                                  cAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGACGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAt     >  1:1169239/1‑150 (MQ=255)
                                                                                                                                                   aGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAtt    <  2:5152415/150‑1 (MQ=255)
                                                                                                                                                     cATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTtc  <  1:4834193/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGGAATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTC  >  CP009273/4297697‑4297995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A