Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F42 I0 R1 87 324.4 10965648 95.0% 10417365 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,297,846 C→A 9.0% M150I (ATG→ATT)  alsE ← allulose‑6‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,297,8460CA 9.0% 1293.4 / 78.7 348M150I (ATG→ATT) alsEallulose‑6‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (154/162);  new base A (16/16);  total (170/178)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 350 total aligned reads displayed.

ACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTC  >  CP009273/4297698‑4297995
                                                                                                                                                    |                                                                                                                                                      
aCAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCa                                                                                                                                                       >  1:3105391/1‑150 (MQ=255)
 cAGGCCGGAAGTGCCGACGATAATGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAt                                                                                                                                                      <  2:1153856/150‑1 (MQ=255)
 cAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAt                                                                                                                                                      <  1:4921359/150‑1 (MQ=255)
 cAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAt                                                                                                                                                      <  1:2199985/150‑1 (MQ=255)
  aGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAtt                                                                                                                                                     <  1:3887811/150‑1 (MQ=255)
   ggCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAttt                                                                                                                                                    <  1:3524618/150‑1 (MQ=255)
   ggCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCAttt                                                                                                                                                    <  1:4653524/150‑1 (MQ=255)
    gCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTc                                                                                                                                                   >  1:4993700/1‑150 (MQ=255)
     ccGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCa                                                                                                                                                  <  1:4784451/150‑1 (MQ=255)
     ccGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCa                                                                                                                                                  <  1:4367690/150‑1 (MQ=255)
       ggAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAgg                                                                                                                                                <  2:4767109/150‑1 (MQ=255)
        gAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCCGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑a                                                                                                                                              >  2:4899537/1‑150 (MQ=255)
         aaGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTATCGTAAGTTGCCTGGTTGCAGGACCCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTAGGCAAGTTTCTCCAGCATTTCAGG‑aa                                                                                                                                             >  1:4682086/1‑150 (MQ=255)
            tGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGa                                                                                                                                          <  2:2837530/150‑1 (MQ=255)
                 acgaTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGt                                                                                                                                     >  1:1461020/1‑150 (MQ=255)
                    aTAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGG‑AATGAACGGTTGt                                                                                                                                  >  1:3367767/1‑150 (MQ=255)
                        aGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCgg                                                                                                                              >  1:1838529/1‑150 (MQ=255)
                         gACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGc                                                                                                                             >  2:4300122/1‑150 (MQ=255)
                          aCATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCa                                                                                                                            >  1:3702392/1‑150 (MQ=255)
                          aCATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCa                                                                                                                            <  2:195619/150‑1 (MQ=255)
                            aTCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCaaa                                                                                                                          >  1:5077493/1‑150 (MQ=255)
                                   ccTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGa                                                                                                                   <  2:4422277/150‑1 (MQ=255)
                                     tGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATc                                                                                                                 <  2:827789/150‑1 (MQ=255)
                                     tGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATc                                                                                                                 <  1:594009/150‑1 (MQ=255)
                                     tGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATc                                                                                                                 <  1:2650140/150‑1 (MQ=255)
                                     tGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATc                                                                                                                 <  2:3354133/150‑1 (MQ=255)
                                        cgccATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGAc                                                                                                              <  2:1270667/150‑1 (MQ=255)
                                           cATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGt                                                                                                           <  2:80666/150‑1 (MQ=255)
                                           cATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGt                                                                                                           >  1:3730560/1‑150 (MQ=255)
                                            aTCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTc                                                                                                          >  2:3596513/1‑150 (MQ=255)
                                                 tttttCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGAc                                                                                                     >  2:5209888/1‑150 (MQ=255)
                                                 tttttCGTAAGTGGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGAc                                                                                                     <  2:3003081/150‑1 (MQ=255)
                                                       gTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAt                                                                                               <  2:1835872/150‑1 (MQ=255)
                                                       gTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAt                                                                                               <  1:3907844/150‑1 (MQ=255)
                                                       gTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACACCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGTATCGACAGTCATGACCGTAAt                                                                                               <  1:3184019/150‑1 (MQ=255)
                                                          aGTTGCCTGGTTGCAGGAACCGTCCACCTCAATGTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAAtttt                                                                                            <  2:4269142/150‑1 (MQ=255)
                                                            ttGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCCGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTAt                                                                                          <  2:1041813/150‑1 (MQ=255)
                                                            ttGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTAt                                                                                          <  2:2323/150‑1 (MQ=255)
                                                             tGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATc                                                                                         <  2:3277200/150‑1 (MQ=255)
                                                             tGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATc                                                                                         <  1:5308988/150‑1 (MQ=255)
                                                              gCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCg                                                                                        <  1:1324798/150‑1 (MQ=255)
                                                               ccTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCgg                                                                                       <  2:1580850/150‑1 (MQ=255)
                                                               ccTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCgg                                                                                       >  1:962352/1‑150 (MQ=255)
                                                                cTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGc                                                                                      <  1:847231/150‑1 (MQ=255)
                                                                   gTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCtt                                                                                   >  2:1325756/1‑150 (MQ=255)
                                                                    ttGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTa                                                                                  >  2:2162291/1‑150 (MQ=255)
                                                                    ttGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTa                                                                                  <  1:1145671/150‑1 (MQ=255)
                                                                       cAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATgg                                                                               <  1:75922/150‑1 (MQ=255)
                                                                         ggAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGat                                                                             >  1:5152961/1‑150 (MQ=255)
                                                                         ggAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGat                                                                             <  1:2059478/150‑1 (MQ=255)
                                                                          gAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGcta                                                                            >  2:3810059/1‑150 (MQ=255)
                                                                          gAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGata                                                                            >  2:2705881/1‑150 (MQ=255)
                                                                            aCCGTCCACCTCAATTTCGTACGCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAATGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTAGGGAtata                                                                          <  2:3530725/150‑1 (MQ=255)
                                                                              cGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGt                                                                        <  1:5444209/150‑1 (MQ=255)
                                                                               gTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTa                                                                       >  2:3885675/1‑150 (MQ=255)
                                                                                tCCACCTCAAGTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAt                                                                      <  1:2892220/150‑1 (MQ=255)
                                                                                 ccACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGAAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTAtt                                                                     <  2:2542811/150‑1 (MQ=255)
                                                                                   aCCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTc                                                                   >  2:2760200/1‑150 (MQ=255)
                                                                                     cTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCAt                                                                 <  2:2249604/150‑1 (MQ=255)
                                                                                      tCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCTTGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATg                                                                <  2:1896409/150‑1 (MQ=255)
                                                                                      tCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCAtt                                                                >  2:4187240/1‑149 (MQ=255)
                                                                                      tCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATg                                                                <  1:1958245/150‑1 (MQ=255)
                                                                                      tCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATg                                                                <  1:3421402/150‑1 (MQ=255)
                                                                                        aaTTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGc                                                              >  2:4600697/1‑150 (MQ=255)
                                                                                        aaTTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGc                                                              >  2:1097080/1‑150 (MQ=255)
                                                                                         aTTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGcc                                                             >  1:1072762/1‑150 (MQ=255)
                                                                                            tCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCa                                                          <  1:234396/150‑1 (MQ=255)
                                                                                            tCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCa                                                          >  1:4482995/1‑150 (MQ=255)
                                                                                            tCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCa                                                          <  2:164315/150‑1 (MQ=255)
                                                                                                  tCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGc                                                    <  1:4296928/150‑1 (MQ=255)
                                                                                                     aGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGtc                                                 <  2:3631180/150‑1 (MQ=255)
                                                                                                      gACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGtct                                                >  1:5273767/1‑150 (MQ=255)
                                                                                                       aCCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGtctc                                               >  2:2448087/1‑150 (MQ=255)
                                                                                                       aCCATCTCGTTCACGCCATGACTTCAGAAAGGAAAGTTTATCCAGCATTACAGG‑AATCAACGGTTGTCCGGCAAAGCCGGGATCGACATTCATGACCTTAATTATAAAGGACTTATGGATATAGAATATCATGGCATCAACTCGCGtctc                                               >  2:5162104/1‑150 (MQ=255)
                                                                                                        ccTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTcc                                              <  1:1407235/150‑1 (MQ=255)
                                                                                                        ccTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTcc                                              <  1:1563870/150‑1 (MQ=255)
                                                                                                         cTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCg                                             <  1:3591262/150‑1 (MQ=255)
                                                                                                           tctcGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCggg                                           >  1:624183/1‑150 (MQ=255)
                                                                                                               gTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTaa                                       >  1:2786708/1‑150 (MQ=255)
                                                                                                                ttCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAg                                      >  2:556083/1‑150 (MQ=255)
                                                                                                                    cGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATc                                  >  1:4778523/1‑150 (MQ=255)
                                                                                                                        aTGCCTTCAGTTCGGCAAGTTTATCCAGAATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGcc                              <  2:4510903/150‑1 (MQ=255)
                                                                                                                          gCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGcccc                            >  2:5033147/1‑150 (MQ=255)
                                                                                                                            cTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCAc                          >  2:619033/1‑150 (MQ=255)
                                                                                                                            cTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCAc                          >  2:2487552/1‑150 (MQ=255)
                                                                                                                              tCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACtt                        <  1:2426766/150‑1 (MQ=255)
                                                                                                                                aGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtc                      <  2:1774468/150‑1 (MQ=255)
                                                                                                                                      gCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtca                <  1:3299405/150‑1 (MQ=255)
                                                                                                                                      gCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtca                >  1:2166873/1‑150 (MQ=255)
                                                                                                                                      gCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtca                >  1:4135615/1‑150 (MQ=255)
                                                                                                                                        aaGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtcatg              >  1:3762539/1‑150 (MQ=255)
                                                                                                                                        aaGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTtcatgtcatg              <  1:4038859/150‑1 (MQ=255)
                                                                                                                                          gTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAc            >  2:5424419/1‑150 (MQ=255)
                                                                                                                                              aTCCAGCAGTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGAcggcg        <  1:3278871/150‑1 (MQ=255)
                                                                                                                                                ccATCATTTCATG‑AATGAACGGTTGTCCGGCAAAGCCGGGATAGACTGTCATGACCGTAATTTTATCGTCCTTATGGATTTATTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGa      <  1:1745122/150‑1 (MQ=255)
                                                                                                                                                ccAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGa      >  2:317123/1‑150 (MQ=255)
                                                                                                                                                  aGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAtt    >  2:2024000/1‑150 (MQ=255)
                                                                                                                                                   gCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAttt   <  1:2571690/150‑1 (MQ=255)
                                                                                                                                                   gCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAttt   >  1:736988/1‑150 (MQ=255)
                                                                                                                                                    cATTTCAGGAAATGAACGGTTGTCCGGCAAAGCCGGGATAGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGAttt   <  2:3702521/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ACAGGCCGGAAGTGCCGACGATAAAGACATCCGCCCCTGCCGCCATCAGTTTTTCGTAAGTTGCCTGGTTGCAGGAACCGTCCACCTCAATTTCGTACTCCAGACCTTCTCGTTCACGCCATGCCTTCAGTTCGGCAAGTTTATCCAGCATTTCAGG‑AATGAACGGTTGTCCGGCAAAGCCGGGATCGACAGTCATGACCGTAATTTTATCGGCCTTATGGATATAGTATTTCATGGCCTCAACTGGCGTCTCCGGGTTAAGGATCAGCCCCACTTTCATGTCATGACGGCGGATTTC  >  CP009273/4297698‑4297995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A