Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,135,016 C→A 24.8% V26V (GTG→GTT)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,135,0160CA24.8% 722.7 / 199.0 301V26V (GTG→GTT) asmAsuppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (97/129);  new base A (40/35);  total (137/164)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

CTTGCGCCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTGCGATATT  >  CP009273/2134867‑2135164
                                                                                                                                                     |                                                                                                                                                    
 ttGCGCCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCa                                                                                                                                                     >  1:5115455/1‑150 (MQ=255)
 ttGCGCCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCa                                                                                                                                                     <  1:3814587/150‑1 (MQ=255)
 ttGCGCCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCa                                                                                                                                                     <  2:3238821/150‑1 (MQ=255)
  tGCGCCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCAc                                                                                                                                                    >  1:2637450/1‑150 (MQ=255)
   gcgcCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCAcc                                                                                                                                                   >  2:1294582/1‑150 (MQ=255)
    cgcCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCa                                                                                                                                                  >  2:2785469/1‑150 (MQ=255)
      cccTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGc                                                                                                                                                <  1:4930464/150‑1 (MQ=255)
       ccTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCa                                                                                                                                               <  1:4858277/150‑1 (MQ=255)
        cTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACTCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCaa                                                                                                                                              <  2:4239012/150‑1 (MQ=255)
          gggCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAaca                                                                                                                                            <  1:2890106/150‑1 (MQ=255)
              ggTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTaa                                                                                                                                        >  2:1388362/1‑150 (MQ=255)
              ggTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTaa                                                                                                                                        <  2:3270994/150‑1 (MQ=255)
               gTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAAc                                                                                                                                       <  2:2253018/150‑1 (MQ=255)
                tGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACg                                                                                                                                      >  1:1844204/1‑150 (MQ=255)
                    agaCATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGa                                                                                                                                  <  1:4150262/150‑1 (MQ=255)
                    agaCATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGa                                                                                                                                  >  2:3608463/1‑150 (MQ=255)
                     gaCATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGAt                                                                                                                                 >  2:1162832/1‑150 (MQ=255)
                      aCATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCACCTTGCTTGACCAGATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTCACGCAGATa                                                                                                                                >  2:3848616/1‑150 (MQ=255)
                      aCATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATa                                                                                                                                <  2:1921913/150‑1 (MQ=255)
                          tCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAAccc                                                                                                                            <  1:1530563/150‑1 (MQ=255)
                          tCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAAccc                                                                                                                            <  2:61680/150‑1 (MQ=255)
                            gCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCgg                                                                                                                          <  2:1739703/150‑1 (MQ=255)
                              ccGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGcc                                                                                                                        >  2:1997167/1‑150 (MQ=255)
                                ggAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGccac                                                                                                                      <  1:4715234/150‑1 (MQ=255)
                                 gagaGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGccacc                                                                                                                     >  2:3191829/1‑150 (MQ=255)
                                   gagGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAg                                                                                                                   <  2:3428932/150‑1 (MQ=255)
                                   gagGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAg                                                                                                                   >  2:2375983/1‑150 (MQ=255)
                                    agGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCTCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGc                                                                                                                  >  1:758623/1‑150 (MQ=255)
                                    agGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGc                                                                                                                  >  2:2725864/1‑150 (MQ=255)
                                    agGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACGTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGc                                                                                                                  >  1:3336035/1‑150 (MQ=255)
                                     ggATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCa                                                                                                                 <  1:196402/150‑1 (MQ=255)
                                     ggATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCa                                                                                                                 >  1:4536229/1‑150 (MQ=255)
                                      gATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCAc                                                                                                                <  1:2996651/150‑1 (MQ=255)
                                      gATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCAc                                                                                                                <  2:2875168/150‑1 (MQ=255)
                                      gATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCAc                                                                                                                >  2:3112383/1‑150 (MQ=255)
                                       aTACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATCGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACg                                                                                                               <  2:3664462/150‑1 (MQ=255)
                                       aTACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACg                                                                                                               >  2:4067581/1‑150 (MQ=255)
                                       aTACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACg                                                                                                               <  2:1100414/150‑1 (MQ=255)
                                         aCTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGAc                                                                                                             <  1:2487459/150‑1 (MQ=255)
                                          cTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATACGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGAcc                                                                                                            <  1:3925353/150‑1 (MQ=255)
                                            aaGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAg                                                                                                          >  2:58993/1‑150 (MQ=255)
                                              gCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGACATCATTCGGATTCCCCAGCTACACGAACGCAGATAACCCGGCCACCAGCACGACCAGGa                                                                                                        >  1:4044138/1‑150 (MQ=255)
                                               cTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAg                                                                                                       <  2:491518/150‑1 (MQ=255)
                                               cTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAg                                                                                                       <  2:4798161/150‑1 (MQ=255)
                                               cTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAg                                                                                                       <  1:364645/150‑1 (MQ=255)
                                                    gCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTatca                                                                                                  <  2:1691106/150‑1 (MQ=255)
                                                     ccAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTatcat                                                                                                 >  1:1056027/1‑150 (MQ=255)
                                                       aGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTAtcatca                                                                                               <  1:133046/150‑1 (MQ=255)
                                                       aGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTAtcatca                                                                                               >  2:692143/1‑150 (MQ=255)
                                                         aCGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGc                                                                                             <  1:496068/150‑1 (MQ=255)
                                                          cGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGcg                                                                                            <  2:102478/150‑1 (MQ=255)
                                                             gCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGcgtcg                                                                                         >  2:1306878/1‑150 (MQ=255)
                                                              ccAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGcgtcgt                                                                                        >  1:696096/1‑150 (MQ=255)
                                                               cAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCgtcgtc                                                                                       >  2:4308254/1‑150 (MQ=255)
                                                                      gTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATc                                                                                <  2:3517026/150‑1 (MQ=255)
                                                                      gTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATc                                                                                <  1:125327/150‑1 (MQ=255)
                                                                        ggCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGt                                                                              <  1:4823765/150‑1 (MQ=255)
                                                                         gCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGtc                                                                             <  2:4969640/150‑1 (MQ=255)
                                                                           ccGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGtctc                                                                           <  1:2417212/150‑1 (MQ=255)
                                                                            cGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCa                                                                          <  2:3320478/150‑1 (MQ=255)
                                                                             gTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCAt                                                                         <  2:4637263/150‑1 (MQ=255)
                                                                              tCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATg                                                                        <  2:4096307/150‑1 (MQ=255)
                                                                                gAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGt                                                                      >  1:3505123/1‑150 (MQ=255)
                                                                                 aGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTa                                                                     >  1:946137/1‑150 (MQ=255)
                                                                                   cTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATc                                                                   <  1:1205424/150‑1 (MQ=255)
                                                                                    tGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATcc                                                                  >  1:3910366/1‑150 (MQ=255)
                                                                                      cAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTc                                                                <  2:3470423/150‑1 (MQ=255)
                                                                                       aaTTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCa                                                               <  1:3608463/150‑1 (MQ=255)
                                                                                        aTTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCaa                                                              <  2:2634493/150‑1 (MQ=255)
                                                                                          tGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTCTCCTCAATg                                                            >  2:1535520/1‑150 (MQ=255)
                                                                                           gATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATgg                                                           <  2:2560068/150‑1 (MQ=255)
                                                                                           gATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATgg                                                           <  2:805006/150‑1 (MQ=255)
                                                                                           gATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATgg                                                           >  1:3772713/1‑150 (MQ=255)
                                                                                            aTAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATggg                                                          >  2:3942676/1‑150 (MQ=255)
                                                                                            aTAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATggg                                                          <  1:3139428/150‑1 (MQ=255)
                                                                                                ccGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTg                                                      >  1:1637598/1‑150 (MQ=255)
                                                                                                 cGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGa                                                     <  2:3695426/150‑1 (MQ=255)
                                                                                                  gCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGaa                                                    <  2:876717/150‑1 (MQ=255)
                                                                                                  gCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGaa                                                    <  2:925427/150‑1 (MQ=255)
                                                                                                   cTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAAt                                                   >  1:3599888/1‑150 (MQ=255)
                                                                                                    tACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAAtt                                                  >  1:4459684/1‑150 (MQ=255)
                                                                                                         gcagcaACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCt                                             >  2:5140130/1‑150 (MQ=255)
                                                                                                          cagcaACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCtt                                            >  1:910455/1‑150 (MQ=255)
                                                                                                           agcaACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCCGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCttt                                           >  1:1833562/1‑150 (MQ=255)
                                                                                                             caACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTAt                                         <  2:198218/150‑1 (MQ=255)
                                                                                                                   gcttgACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTc                                   <  1:3245875/150‑1 (MQ=255)
                                                                                                                   gcttgACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTACCCTCAATGGGCCTGAATTAGTCTTTATCGATTc                                   <  2:2445277/150‑1 (MQ=255)
                                                                                                                    cttgACCCTATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCg                                  <  1:2104757/150‑1 (MQ=255)
                                                                                                                             ataGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTAc                         <  2:2173944/150‑1 (MQ=255)
                                                                                                                              taGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTAcc                        <  2:2278428/150‑1 (MQ=255)
                                                                                                                                 tCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACcgcg                     <  1:4302009/150‑1 (MQ=255)
                                                                                                                                  cgcgGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCgcgc                    >  1:2025530/1‑150 (MQ=255)
                                                                                                                                   gcgGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGcc                   >  1:1004780/1‑150 (MQ=255)
                                                                                                                                    cgGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGccc                  <  2:4448289/150‑1 (MQ=255)
                                                                                                                                     ggAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCct                 >  1:2767328/1‑150 (MQ=255)
                                                                                                                                       aaaTCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCctgc               >  1:80629/1‑150 (MQ=255)
                                                                                                                                          tCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTgg            <  1:5004922/150‑1 (MQ=255)
                                                                                                                                          tCAGTCGGATTCCCCCGCCCCCCTCCCGCAGAGAACCCGGCCCCCCGCACGACCAGGAGTATCATCCGCGTCGTCAGAAATCGGCTCATGGTATCCTCCCTGGGCCTGACTTAGTCTTTCGCGCGGCGGCTGGCGCCCGCGCCCTGCggg            <  2:72591/150‑1 (MQ=255)
                                                                                                                                            aTTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCATCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGtt          >  2:3185805/1‑150 (MQ=255)
                                                                                                                                                 gATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTGCGat     >  2:2530569/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTTGCGCCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTGCGATATT  >  CP009273/2134867‑2135164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A