Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,135,016 C→A 12.5% V26V (GTG→GTT)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,135,0160CA12.5% 824.0 / 64.5 239V26V (GTG→GTT) asmAsuppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (88/121);  new base A (10/20);  total (98/141)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 241 total aligned reads displayed.

CTTGCGCCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTGCGAT  >  CP009273/2134867‑2135161
                                                                                                                                                     |                                                                                                                                                 
 ttGCGCCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCa                                                                                                                                                  <  1:2650416/150‑1 (MQ=255)
    cgcCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCa                                                                                                                                               >  1:3627639/1‑150 (MQ=255)
       ccTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCa                                                                                                                                            >  1:3520515/1‑150 (MQ=255)
       ccTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCa                                                                                                                                            <  2:3192788/150‑1 (MQ=255)
       ccTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCa                                                                                                                                            <  2:4302954/150‑1 (MQ=255)
         tGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAac                                                                                                                                          >  2:2918512/1‑150 (MQ=255)
         tGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAac                                                                                                                                          >  1:1472682/1‑150 (MQ=255)
          gggCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAaca                                                                                                                                         >  2:2993429/1‑150 (MQ=255)
            gCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACt                                                                                                                                       <  2:1510999/150‑1 (MQ=255)
             cGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTa                                                                                                                                      <  1:58063/150‑1 (MQ=255)
                tGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACg                                                                                                                                   <  1:618795/150‑1 (MQ=255)
                tGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACg                                                                                                                                   >  1:2755515/1‑150 (MQ=255)
                  agagaCATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCa                                                                                                                                 >  1:3752857/1‑150 (MQ=255)
                   gagaCATTCGCCCGGAGAGGATACTAAGCTGCGGTCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATTACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAg                                                                                                                                <  1:462234/150‑1 (MQ=255)
                    agaCATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGa                                                                                                                               <  2:3458317/150‑1 (MQ=255)
                      aCATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATa                                                                                                                             <  1:3142476/150‑1 (MQ=255)
                        aTTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAAc                                                                                                                           <  2:460316/150‑1 (MQ=255)
                        aTTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAAc                                                                                                                           <  2:4524023/150‑1 (MQ=255)
                         ttCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAAcc                                                                                                                          >  1:1613781/1‑150 (MQ=255)
                          tCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAAccc                                                                                                                         >  2:2959396/1‑150 (MQ=255)
                              ccGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGcc                                                                                                                     >  1:735671/1‑150 (MQ=255)
                               cGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGcca                                                                                                                    <  2:1140728/150‑1 (MQ=255)
                               cGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGcca                                                                                                                    >  2:3099344/1‑150 (MQ=255)
                               cGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGcca                                                                                                                    <  2:2144563/150‑1 (MQ=255)
                                 gagaGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGccacc                                                                                                                  >  1:856604/1‑150 (MQ=255)
                                   gagGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAg                                                                                                                <  2:4373287/150‑1 (MQ=255)
                                     ggATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCa                                                                                                              >  2:3036720/1‑150 (MQ=255)
                                         aCTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGAc                                                                                                          >  2:4278902/1‑150 (MQ=255)
                                             aGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAgg                                                                                                      >  1:1006113/1‑150 (MQ=255)
                                                  cGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTat                                                                                                 <  2:2739500/150‑1 (MQ=255)
                                                   ggCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTatc                                                                                                <  2:1603859/150‑1 (MQ=255)
                                                     ccAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTatcat                                                                                              <  1:3505277/150‑1 (MQ=255)
                                                           gTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGcgt                                                                                        <  2:554325/150‑1 (MQ=255)
                                                            tGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGcgtc                                                                                       >  2:2061728/1‑150 (MQ=255)
                                                             gCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGcgtcg                                                                                      <  1:2804472/150‑1 (MQ=255)
                                                               cAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCgtcgtc                                                                                    >  2:961846/1‑150 (MQ=255)
                                                                      gTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATc                                                                             <  1:2908390/150‑1 (MQ=255)
                                                                      gTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATc                                                                             <  2:3986982/150‑1 (MQ=255)
                                                                       tGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCg                                                                            <  2:2351522/150‑1 (MQ=255)
                                                                       tGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCg                                                                            <  2:2351927/150‑1 (MQ=255)
                                                                       tGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCg                                                                            >  2:806831/1‑150 (MQ=255)
                                                                       tGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCg                                                                            >  2:1720611/1‑150 (MQ=255)
                                                                        ggCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGt                                                                           <  2:1808353/150‑1 (MQ=255)
                                                                           ccGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGtctc                                                                        <  1:4302547/150‑1 (MQ=255)
                                                                            cGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCa                                                                       >  1:3013479/1‑150 (MQ=255)
                                                                             gTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCAt                                                                      >  1:687624/1‑150 (MQ=255)
                                                                             gTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCAt                                                                      <  2:3124570/150‑1 (MQ=255)
                                                                                gAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGt                                                                   >  2:3205/1‑150 (MQ=255)
                                                                                gAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGt                                                                   >  2:222871/1‑150 (MQ=255)
                                                                                gAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGACCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGt                                                                   >  1:3968392/1‑150 (MQ=255)
                                                                                gAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGt                                                                   <  2:2619265/150‑1 (MQ=255)
                                                                                 aGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTa                                                                  <  1:3889682/150‑1 (MQ=255)
                                                                                 aGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTa                                                                  <  1:3364492/150‑1 (MQ=255)
                                                                                  gCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCTTCTCATGGTAt                                                                 >  1:4584869/1‑150 (MQ=255)
                                                                                  gCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTAt                                                                 <  1:2166157/150‑1 (MQ=255)
                                                                                   cTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGAGTCACCAGCAACACTAACGCAGATACCCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATc                                                                <  1:1169183/150‑1 (MQ=255)
                                                                                      cAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTc                                                             <  1:3117251/150‑1 (MQ=255)
                                                                                       aaTTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCa                                                            >  1:1083680/1‑150 (MQ=255)
                                                                                       aaTTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCa                                                            <  1:2345391/150‑1 (MQ=255)
                                                                                       aaTTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCa                                                            >  2:373736/1‑150 (MQ=255)
                                                                                       aaTTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATAAGTCGGATTCACCAGCAACACTAACGCAGATAACACGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTCTCCTCa                                                            >  2:1003924/1‑150 (MQ=255)
                                                                                        aTTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCaa                                                           >  2:3391291/1‑150 (MQ=255)
                                                                                         ttGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAAt                                                          <  2:1184717/150‑1 (MQ=255)
                                                                                            aTAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATggg                                                       >  2:3569447/1‑150 (MQ=255)
                                                                                             tAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGc                                                      <  2:280752/150‑1 (MQ=255)
                                                                                               aCCGCTTCGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCt                                                    <  1:822553/150‑1 (MQ=255)
                                                                                                ccGCTACGTGCAGCAACCTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTg                                                   >  1:4560174/1‑150 (MQ=255)
                                                                                                   cTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAAt                                                <  1:2074694/150‑1 (MQ=255)
                                                                                                   cTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAAt                                                >  2:3494052/1‑150 (MQ=255)
                                                                                                     aCGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTa                                              >  2:4449800/1‑150 (MQ=255)
                                                                                                        tGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTc                                           <  2:794525/150‑1 (MQ=255)
                                                                                                         gcagcaACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCt                                          <  1:4493363/150‑1 (MQ=255)
                                                                                                          cagcaACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCtt                                         <  2:4054145/150‑1 (MQ=255)
                                                                                                          cagcaACGTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCtt                                         <  2:786622/150‑1 (MQ=255)
                                                                                                           agcaACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCttt                                        >  2:3352852/1‑150 (MQ=255)
                                                                                                             caACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTAt                                      <  1:2362409/150‑1 (MQ=255)
                                                                                                             caACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTAt                                      <  1:1901864/150‑1 (MQ=255)
                                                                                                             caACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTAt                                      <  2:2981040/150‑1 (MQ=255)
                                                                                                             caACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTAt                                      <  2:1756033/150‑1 (MQ=255)
                                                                                                              aaCTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATc                                     >  2:4616565/1‑150 (MQ=255)
                                                                                                                  tgcttgACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGAtt                                 >  1:1404171/1‑150 (MQ=255)
                                                                                                                     ttgACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCgg                              >  2:1036729/1‑150 (MQ=255)
                                                                                                                      tgACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCggc                             <  2:1347512/150‑1 (MQ=255)
                                                                                                                       gACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCggct                            <  1:145792/150‑1 (MQ=255)
                                                                                                                         ccATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCggctgg                          <  2:492256/150‑1 (MQ=255)
                                                                                                                           atatAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCggctggct                        <  2:60957/150‑1 (MQ=255)
                                                                                                                              taGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTAcc                     <  2:1483428/150‑1 (MQ=255)
                                                                                                                               aGTCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACcg                    >  1:2153012/1‑150 (MQ=255)
                                                                                                                                 tCGCGGAAATCATTCGGATTAACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACcgcg                  >  2:4019314/1‑150 (MQ=255)
                                                                                                                                  cgcgGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCgcgc                 >  1:2191464/1‑150 (MQ=255)
                                                                                                                                    cgGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGccc               <  1:1672273/150‑1 (MQ=255)
                                                                                                                                     ggAAATCATTCGGATTCACCAGCAACACTTACTGAGATAACCCGGCCACCAGCACGACCAGGAGTTTCTTCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCct              <  1:195321/150‑1 (MQ=255)
                                                                                                                                     ggAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCct              <  1:1231331/150‑1 (MQ=255)
                                                                                                                                      gAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCTATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCctg             <  2:2300988/150‑1 (MQ=255)
                                                                                                                                         aTCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTAAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCctgctg          <  1:1850421/150‑1 (MQ=255)
                                                                                                                                            aTTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGtt       <  1:3011936/150‑1 (MQ=255)
                                                                                                                                            aTTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGtt       <  1:3012067/150‑1 (MQ=255)
                                                                                                                                            aTTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGtt       <  1:3940940/150‑1 (MQ=255)
                                                                                                                                             ttCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTg      <  1:586765/150‑1 (MQ=255)
                                                                                                                                                ggATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTGCGa   >  2:3284980/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CTTGCGCCCTGGGCGGTGAGAGACATTCGCCCGGAGAGGATACTAAGCTGCGGCCAGACGTGCCAACGCAGTGGCCCGTCGAGCTGCAATTGATAACCGCTACGTGCAGCAACTTGCTTGACCATATAGTCGCGGAAATCATTCGGATTCACCAGCAACACTAACGCAGATAACCCGGCCACCAGCACGACCAGGAGTATCATCAGCGTCGTCAGAAATCGTCTCATGGTATCCTCAATGGGCCTGAATTAGTCTTTATCGATTCGGCTGGCTACCGCGCCCTGCTGGTTGCGAT  >  CP009273/2134867‑2135161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A