Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,163,087 C→A 29.2% R299S (CGT→AGT)  yegS → phosphatidylglycerol kinase, metal‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,163,0870CA29.2% 545.7 / 211.4 266R299S (CGT→AGT) yegSphosphatidylglycerol kinase, metal‑dependent
Reads supporting (aligned to +/- strand):  ref base C (85/103);  new base A (36/42);  total (121/145)
Fisher's exact test for biased strand distribution p-value = 8.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

TCGAAGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAG  >  CP009273/2162938‑2163235
                                                                                                                                                     |                                                                                                                                                    
tCGAAGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGc                                                                                                                                                      >  1:3003456/1‑150 (MQ=255)
 cGAAGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCg                                                                                                                                                     <  2:772016/150‑1 (MQ=255)
    aGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTa                                                                                                                                                  >  2:4146102/1‑150 (MQ=255)
    aGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTa                                                                                                                                                  >  2:3370223/1‑150 (MQ=255)
    aGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTa                                                                                                                                                  >  2:3968258/1‑150 (MQ=255)
     ggCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTaa                                                                                                                                                 >  1:1088168/1‑150 (MQ=255)
      gcgcTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAAt                                                                                                                                                <  2:1289421/150‑1 (MQ=255)
       cgcTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATc                                                                                                                                               >  2:5126717/1‑150 (MQ=255)
          ttCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCaaa                                                                                                                                            <  1:1027185/150‑1 (MQ=255)
          ttCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCaaa                                                                                                                                            >  1:3500029/1‑150 (MQ=255)
            cgtcgtGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACt                                                                                                                                          >  1:3855600/1‑150 (MQ=255)
             gtcgtGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTa                                                                                                                                         >  2:4482670/1‑150 (MQ=255)
              tcgtGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTAt                                                                                                                                        >  2:4071211/1‑150 (MQ=255)
               cgtGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTAtt                                                                                                                                       >  1:1241168/1‑150 (MQ=255)
               cgtGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTAtt                                                                                                                                       <  1:2731997/150‑1 (MQ=255)
                 tGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATtct                                                                                                                                     >  2:3720139/1‑150 (MQ=255)
                  ggTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATtctc                                                                                                                                    >  2:1699344/1‑150 (MQ=255)
                    tttGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATtctctc                                                                                                                                  <  1:265061/150‑1 (MQ=255)
                    tttGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATtctctc                                                                                                                                  <  2:2417658/150‑1 (MQ=255)
                    tttGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATtctctc                                                                                                                                  <  1:2861037/150‑1 (MQ=255)
                     ttGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTctctct                                                                                                                                 <  1:3249626/150‑1 (MQ=255)
                      tGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCtt                                                                                                                                <  2:4784151/150‑1 (MQ=255)
                         tatTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGaa                                                                                                                             <  1:2124220/150‑1 (MQ=255)
                          atTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAAc                                                                                                                            >  1:4160096/1‑150 (MQ=255)
                            tCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCa                                                                                                                          <  1:1408654/150‑1 (MQ=255)
                             cAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAg                                                                                                                         >  1:4192240/1‑150 (MQ=255)
                               ggCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAggg                                                                                                                       <  2:1464318/150‑1 (MQ=255)
                                gCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGga                                                                                                                      <  2:2336971/150‑1 (MQ=255)
                                  accacACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGgaga                                                                                                                    >  1:888754/1‑150 (MQ=255)
                                   ccacACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGaa                                                                                                                   <  2:4667290/150‑1 (MQ=255)
                                      cacGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATcc                                                                                                                <  1:4204020/150‑1 (MQ=255)
                                          aCATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTaa                                                                                                            >  1:3673305/1‑150 (MQ=255)
                                             tCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGc                                                                                                         <  1:3728420/150‑1 (MQ=255)
                                              cACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGcc                                                                                                        >  1:5087616/1‑150 (MQ=255)
                                                ccTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCgg                                                                                                      <  2:1431439/150‑1 (MQ=255)
                                                   ttAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATg                                                                                                   >  1:2249612/1‑150 (MQ=255)
                                                       tCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGgt                                                                                               <  2:4437668/150‑1 (MQ=255)
                                                        cTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGgtg                                                                                              >  2:3606145/1‑150 (MQ=255)
                                                          tGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTa                                                                                            <  1:2303963/150‑1 (MQ=255)
                                                             tGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTacac                                                                                         >  2:4933038/1‑150 (MQ=255)
                                                             tGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAac                                                                                         <  2:856512/150‑1 (MQ=255)
                                                                    ccGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTAt                                                                                  <  2:3475471/150‑1 (MQ=255)
                                                                     cGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGTTTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACTCCTTATc                                                                                 <  1:175886/150‑1 (MQ=255)
                                                                             gggCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATAc                                                                         <  1:917460/150‑1 (MQ=255)
                                                                             gggCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATAc                                                                         >  2:4334442/1‑150 (MQ=255)
                                                                                 aaaaTTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAATCACCTTATCCGGCATACAGaa                                                                     >  1:1445279/1‑150 (MQ=255)
                                                                                    aTTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACaa                                                                  >  2:673725/1‑150 (MQ=255)
                                                                                    aTTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACaa                                                                  <  1:3141593/150‑1 (MQ=255)
                                                                                     ttttCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAAt                                                                 >  2:1506187/1‑150 (MQ=255)
                                                                                      tttCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAAta                                                                <  1:2446803/150‑1 (MQ=255)
                                                                                        tCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAAtact                                                              <  1:3181264/150‑1 (MQ=255)
                                                                                        tCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAAtact                                                              <  2:1898030/150‑1 (MQ=255)
                                                                                        tCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAAtact                                                              <  2:1837809/150‑1 (MQ=255)
                                                                                          atatTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAAtactac                                                            <  2:2541015/150‑1 (MQ=255)
                                                                                              tGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCg                                                        <  2:3291643/150‑1 (MQ=255)
                                                                                              tGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCg                                                        <  1:250749/150‑1 (MQ=255)
                                                                                               gAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGt                                                       >  1:4839319/1‑150 (MQ=255)
                                                                                                aaaTACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGta                                                      >  1:429672/1‑150 (MQ=255)
                                                                                                aaaTACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGta                                                      <  1:1622974/150‑1 (MQ=255)
                                                                                                aaaTACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGta                                                      >  2:2804711/1‑150 (MQ=255)
                                                                                                aaaTACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGta                                                      <  2:2008011/150‑1 (MQ=255)
                                                                                                aaaTACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGta                                                      >  1:904939/1‑150 (MQ=255)
                                                                                                 aaTACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGtaa                                                     <  1:1901134/150‑1 (MQ=255)
                                                                                                   tACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGtaatt                                                   <  2:1479870/150‑1 (MQ=255)
                                                                                                    aCTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGtaatta                                                  >  1:4780976/1‑150 (MQ=255)
                                                                                                     cTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGtaattaa                                                 <  2:1375396/150‑1 (MQ=255)
                                                                                                     cTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGtaattaa                                                 >  2:4271743/1‑150 (MQ=255)
                                                                                                        ccGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAAttt                                              <  1:474247/150‑1 (MQ=255)
                                                                                                        ccGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAAttt                                              <  1:4412367/150‑1 (MQ=255)
                                                                                                         cGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAAtttt                                             <  1:1699361/150‑1 (MQ=255)
                                                                                                           gCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTAc                                           <  1:660122/150‑1 (MQ=255)
                                                                                                           gCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTAc                                           <  1:4498447/150‑1 (MQ=255)
                                                                                                             aGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTAccc                                         >  1:4899412/1‑150 (MQ=255)
                                                                                                              gcgttgcgttGTCGATTACCACCAGAGTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCcg                                        <  2:3124499/150‑1 (MQ=255)
                                                                                                                  tgcgttgTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGtc                                    >  2:2178848/1‑150 (MQ=255)
                                                                                                                      ttgTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGtcttct                                <  1:3015457/150‑1 (MQ=255)
                                                                                                                        gTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGc                              <  2:198455/150‑1 (MQ=255)
                                                                                                                           gATTACCACCAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAAt                           <  2:908597/150‑1 (MQ=255)
                                                                                                                               accaccAGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCa                       <  1:2076367/150‑1 (MQ=255)
                                                                                                                                  accaGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGtt                    >  1:3575451/1‑150 (MQ=255)
                                                                                                                                  accaGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGtt                    >  2:1048957/1‑150 (MQ=255)
                                                                                                                                   ccaGATTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGttt                   >  1:4986326/1‑150 (MQ=255)
                                                                                                                                     aGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTAt                 >  2:1950805/1‑150 (MQ=255)
                                                                                                                                      gATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATc                <  2:2682830/150‑1 (MQ=255)
                                                                                                                                      gATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATc                >  1:3801928/1‑150 (MQ=255)
                                                                                                                                      gATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATc                <  1:3874817/150‑1 (MQ=255)
                                                                                                                                      gATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATc                >  1:1592679/1‑150 (MQ=255)
                                                                                                                                       aTTGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATcc               <  1:2819628/150‑1 (MQ=255)
                                                                                                                                         tGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCAc             >  1:2945170/1‑150 (MQ=255)
                                                                                                                                         tGTCCACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCAc             >  1:857678/1‑150 (MQ=255)
                                                                                                                                           tCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACct           >  1:1148349/1‑150 (MQ=255)
                                                                                                                                             cACTGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCTTAATGCCGGATGCTTTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCTTCTTCTGCAATGCCAGTTTATCCACctct         <  1:41436/150‑1 (MQ=255)
                                                                                                                                                tGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGac      >  1:3706357/1‑150 (MQ=255)
                                                                                                                                                tGTTGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGac      <  1:3370223/150‑1 (MQ=255)
                                                                                                                                                 gTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGaca     >  1:612250/1‑150 (MQ=255)
                                                                                                                                                  ttGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGacac    >  2:2620897/1‑150 (MQ=255)
                                                                                                                                                  ttGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTCCTGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGacac    <  2:116214/150‑1 (MQ=255)
                                                                                                                                                  ttGAGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGacac    <  2:3881978/150‑1 (MQ=255)
                                                                                                                                                   tGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGAcaca   >  1:2583224/1‑150 (MQ=255)
                                                                                                                                                    gCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAg  <  1:2139751/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCGAAGGCGCTTCGTCGTGGTTTGATATTCAGGCACCACACGACATCACCTTTAATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAG  >  CP009273/2162938‑2163235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A