Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,193,783 C→A 16.6% A9E (GCG→GAG)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,193,7830CA16.6% 917.1 / 117.1 299A9E (GCG→GAG) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (116/133);  new base A (31/19);  total (147/152)
Fisher's exact test for biased strand distribution p-value = 6.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

CAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAA  >  CP009273/2193634‑2193931
                                                                                                                                                     |                                                                                                                                                    
cAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGtggctggc                                                                                                                                                      <  2:4688692/150‑1 (MQ=255)
     cAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATa                                                                                                                                                 >  1:119462/1‑150 (MQ=255)
     cAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATa                                                                                                                                                 <  2:2880196/150‑1 (MQ=255)
      aGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATaa                                                                                                                                                <  1:5146814/150‑1 (MQ=255)
       gAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAAc                                                                                                                                               <  2:4739058/150‑1 (MQ=255)
        aaaGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACg                                                                                                                                              >  2:4558128/1‑150 (MQ=255)
          aGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGcc                                                                                                                                            <  1:4362510/150‑1 (MQ=255)
           gATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTCGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGccc                                                                                                                                           <  1:4098652/150‑1 (MQ=255)
            atatGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCa                                                                                                                                          >  2:597406/1‑150 (MQ=255)
               tGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACt                                                                                                                                       <  1:1553580/150‑1 (MQ=255)
                ggCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTg                                                                                                                                      <  2:4480321/150‑1 (MQ=255)
                 gCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTgg                                                                                                                                     <  2:3810450/150‑1 (MQ=255)
                   tGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGaa                                                                                                                                   <  1:3056645/150‑1 (MQ=255)
                    gggggAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAAc                                                                                                                                  <  2:1753916/150‑1 (MQ=255)
                     ggggAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACt                                                                                                                                 >  1:561231/1‑150 (MQ=255)
                       ggAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGa                                                                                                                               <  1:4916522/150‑1 (MQ=255)
                        gAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGaa                                                                                                                              <  1:2319479/150‑1 (MQ=255)
                         aGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGaaa                                                                                                                             >  1:581688/1‑150 (MQ=255)
                           tAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAata                                                                                                                           >  1:3520670/1‑150 (MQ=255)
                            aaTTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAatat                                                                                                                          >  2:2162389/1‑150 (MQ=255)
                             aTTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAAtata                                                                                                                         >  2:5094519/1‑150 (MQ=255)
                                cAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATaaca                                                                                                                      >  2:1529081/1‑148 (MQ=255)
                                   cacaGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATTGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAg                                                                                                                   <  2:4311014/150‑1 (MQ=255)
                                     caGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGc                                                                                                                 <  1:3077353/150‑1 (MQ=255)
                                     caGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGc                                                                                                                 <  1:3781004/150‑1 (MQ=255)
                                      aGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCa                                                                                                                <  1:3030649/150‑1 (MQ=255)
                                      aGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCa                                                                                                                <  2:1131648/150‑1 (MQ=255)
                                          aGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAAc                                                                                                            <  1:2566426/150‑1 (MQ=255)
                                          aGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAAc                                                                                                            <  2:2969785/150‑1 (MQ=255)
                                           gcgcAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAAcg                                                                                                           <  1:1284790/150‑1 (MQ=255)
                                             gcAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAAcgcc                                                                                                         <  1:4156575/150‑1 (MQ=255)
                                                gTTCTCCGTAATCTAGGCAATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTTAATAAGTAAAGAGCATGGACAAGGAATTACCGTGGCGGGCGGATAACGCCCAACTGTAACTGAAATATAAAAAAGGCAACACGCCGCt                                                                                                      <  2:3032778/150‑1 (MQ=255)
                                                  tctcCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCa                                                                                                    <  2:2102400/150‑1 (MQ=255)
                                                  tctcCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCa                                                                                                    <  1:4246346/150‑1 (MQ=255)
                                                    tcCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGt                                                                                                  <  2:3555543/150‑1 (MQ=255)
                                                       gTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGCAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCAt                                                                                               <  2:1811758/150‑1 (MQ=255)
                                                        tAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAACCGCCGCTCAGTCAtc                                                                                              <  2:1579507/150‑1 (MQ=255)
                                                        tAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCAtc                                                                                              >  2:2391407/1‑150 (MQ=255)
                                                        tAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCAtc                                                                                              >  1:2794480/1‑150 (MQ=255)
                                                           tCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAAcgccgc                                                                                                       <  2:1483539/138‑1 (MQ=255)
                                                           tCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCAtcgtc                                                                                           <  1:4889079/150‑1 (MQ=255)
                                                              aTGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCt                                                                                        >  2:4666345/1‑150 (MQ=255)
                                                               tGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTg                                                                                       >  2:3386045/1‑150 (MQ=255)
                                                               tGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTg                                                                                       <  2:3051152/150‑1 (MQ=255)
                                                               tGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTg                                                                                       <  1:3256840/150‑1 (MQ=255)
                                                               tGCCATCACCGCCAGAGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTg                                                                                       <  2:498454/150‑1 (MQ=255)
                                                                  cATCCCCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTCCCGTGGCGGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGcc                                                                                    <  1:502132/150‑1 (MQ=255)
                                                                   aTCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCg                                                                                   >  1:3182725/1‑150 (MQ=255)
                                                                    tCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCgg                                                                                  >  1:1818868/1‑150 (MQ=255)
                                                                     cACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCggg                                                                                 >  2:254422/1‑150 (MQ=255)
                                                                            aGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCa                                                                          <  2:86306/150‑1 (MQ=255)
                                                                                   tAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCg                                                                   >  1:1914345/1‑150 (MQ=255)
                                                                                    aaTTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGt                                                                  >  2:2024539/1‑150 (MQ=255)
                                                                                       tAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTAt                                                               >  1:5111193/1‑150 (MQ=255)
                                                                                         aCGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCa                                                             <  2:949804/150‑1 (MQ=255)
                                                                                         aCGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCa                                                             >  2:2622575/1‑150 (MQ=255)
                                                                                                tGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAg                                                      <  1:2409853/150‑1 (MQ=255)
                                                                                                tGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAg                                                      >  1:3712990/1‑150 (MQ=255)
                                                                                                 gAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGt                                                     >  2:4505525/1‑150 (MQ=255)
                                                                                                 gAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGt                                                     >  1:641765/1‑150 (MQ=255)
                                                                                                  aaGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGtc                                                    >  2:974681/1‑150 (MQ=255)
                                                                                                  aaGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGtc                                                    >  1:1119834/1‑150 (MQ=255)
                                                                                                     cccTGTTTGAATAAGGAAAGAGCATGGACAAGTAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCa                                                 <  1:2757827/150‑1 (MQ=255)
                                                                                                       cTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATc                                               >  2:3143649/1‑150 (MQ=255)
                                                                                                        tGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATcc                                              <  1:1051947/150‑1 (MQ=255)
                                                                                                        tGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATcc                                              <  1:4558128/150‑1 (MQ=255)
                                                                                                         gTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCa                                             <  2:670479/150‑1 (MQ=255)
                                                                                                          tttGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAg                                            <  1:4372886/150‑1 (MQ=255)
                                                                                                           ttGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGa                                           <  1:2043010/150‑1 (MQ=255)
                                                                                                             gAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACa                                         >  2:3662050/1‑150 (MQ=255)
                                                                                                              aaTAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACAt                                        >  1:783953/1‑150 (MQ=255)
                                                                                                              aaTAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACAt                                        >  1:3847008/1‑150 (MQ=255)
                                                                                                                tAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTg                                      >  1:696973/1‑150 (MQ=255)
                                                                                                                tAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTg                                      <  1:2908642/150‑1 (MQ=255)
                                                                                                                   ggAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGt                                   <  1:1813076/150‑1 (MQ=255)
                                                                                                                   ggAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGt                                   <  1:3622215/150‑1 (MQ=255)
                                                                                                                     aaaGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGtga                                 >  1:3199844/1‑150 (MQ=255)
                                                                                                                       agagCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGtgatg                               <  1:1456210/150‑1 (MQ=255)
                                                                                                                       agagCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGtgatg                               >  2:592344/1‑150 (MQ=255)
                                                                                                                        gagCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGtgatga                              >  1:97188/1‑150 (MQ=255)
                                                                                                                         agCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGaa                             <  1:2073043/150‑1 (MQ=255)
                                                                                                                           cATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAAtt                           >  2:506740/1‑150 (MQ=255)
                                                                                                                            aTGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTg                          >  2:4816423/1‑150 (MQ=255)
                                                                                                                            aTGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTg                          >  2:2463266/1‑150 (MQ=255)
                                                                                                                                aCAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTAc                      <  1:1150684/150‑1 (MQ=255)
                                                                                                                                 cacggAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACa                     <  1:4823334/147‑1 (MQ=255)
                                                                                                                                 cAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACa                     >  2:4132315/1‑150 (MQ=255)
                                                                                                                                  aaGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACaa                    >  1:5096432/1‑150 (MQ=255)
                                                                                                                                   aGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACaaa                   <  2:2350600/150‑1 (MQ=255)
                                                                                                                                   aGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACaaa                   <  2:1375495/150‑1 (MQ=255)
                                                                                                                                       aTTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGc               >  1:3648951/1‑150 (MQ=255)
                                                                                                                                          aCCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTga            <  1:4100935/150‑1 (MQ=255)
                                                                                                                                           ccGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTgag           <  1:2162389/150‑1 (MQ=255)
                                                                                                                                            cGTGGCTGGCTGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTgaga          <  2:119462/150‑1 (MQ=255)
                                                                                                                                             gTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGagaa         <  2:4343205/150‑1 (MQ=255)
                                                                                                                                              tggctggcGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGagaag        <  1:646136/150‑1 (MQ=255)
                                                                                                                                               ggctggcGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGagaaga       <  1:3107879/150‑1 (MQ=255)
                                                                                                                                                   ggcGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaa   <  1:259199/150‑1 (MQ=255)
                                                                                                                                                   ggcGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaa   >  1:3098629/1‑150 (MQ=255)
                                                                                                                                                    gcGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaa  <  1:3420418/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAA  >  CP009273/2193634‑2193931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A