Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,193,783 C→A 21.6% A9E (GCG→GAG)  yehI → DUF4132 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,193,7830CA21.6% 614.1 / 139.8 257A9E (GCG→GAG) yehIDUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (100/99);  new base A (34/22);  total (134/123)
Fisher's exact test for biased strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01

Only 100 of 270 total aligned reads displayed.

CAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAA  >  CP009273/2193634‑2193932
                                                                                                                                                     |                                                                                                                                                     
cAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGtggctggc                                                                                                                                                       >  2:152507/1‑150 (MQ=255)
  aaGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCgg                                                                                                                                                     >  1:2801925/1‑150 (MQ=255)
    gCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGAt                                                                                                                                                   <  2:2108482/150‑1 (MQ=255)
      aGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATaa                                                                                                                                                 >  2:1379880/1‑150 (MQ=255)
      aGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATaa                                                                                                                                                 >  2:3462063/1‑150 (MQ=255)
       gAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAAc                                                                                                                                                <  2:4610900/150‑1 (MQ=255)
        aaaGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACg                                                                                                                                               <  1:1500506/150‑1 (MQ=255)
         aaGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGc                                                                                                                                              >  1:3356514/1‑150 (MQ=255)
           gATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGccc                                                                                                                                            <  2:4030065/150‑1 (MQ=255)
           gATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGccc                                                                                                                                            >  2:433472/1‑150 (MQ=255)
                ggCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTg                                                                                                                                       <  2:1308102/150‑1 (MQ=255)
                ggCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTg                                                                                                                                       >  1:372918/1‑150 (MQ=255)
                  cTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGa                                                                                                                                     <  1:3651355/150‑1 (MQ=255)
                    gggggAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAAc                                                                                                                                   <  2:737362/150‑1 (MQ=255)
                     ggggggTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACt                                                                                                                                  <  2:377579/150‑1 (MQ=255)
                         aGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGaaa                                                                                                                              >  1:3682383/1‑150 (MQ=255)
                          gTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAat                                                                                                                             >  2:3632132/1‑150 (MQ=255)
                           tAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAata                                                                                                                            <  1:1525806/150‑1 (MQ=255)
                            aaTTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAatat                                                                                                                           >  1:1778001/1‑150 (MQ=255)
                             aTTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAAtata                                                                                                                          <  2:4542435/150‑1 (MQ=255)
                             aTTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAAtata                                                                                                                          <  2:3618420/150‑1 (MQ=255)
                                cAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATaaaa                                                                                                                       >  1:2618784/1‑150 (MQ=255)
                                  acacAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATaaaaaa                                                                                                                     >  2:3281173/1‑150 (MQ=255)
                                   cacaGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAg                                                                                                                    <  1:2995560/150‑1 (MQ=255)
                                   cacaGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAg                                                                                                                    <  1:3400932/150‑1 (MQ=255)
                                   cacaGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAg                                                                                                                    <  1:1805957/150‑1 (MQ=255)
                                     caGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGc                                                                                                                  >  2:103979/1‑150 (MQ=255)
                                        gAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCaaa                                                                                                               <  2:2642616/150‑1 (MQ=255)
                                        gAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCaaa                                                                                                               <  1:4502168/150‑1 (MQ=255)
                                         aaGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCaaaa                                                                                                              >  2:3097295/1‑150 (MQ=255)
                                          aGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAAc                                                                                                             <  2:529197/150‑1 (MQ=255)
                                          aGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAAc                                                                                                             <  2:4790648/150‑1 (MQ=255)
                                           gcgcAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAAcg                                                                                                            <  1:2603428/150‑1 (MQ=255)
                                             gcAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAAcgcc                                                                                                          >  2:2828815/1‑150 (MQ=255)
                                               aGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAAcgccgc                                                                                                        >  1:1267979/1‑150 (MQ=255)
                                               aGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAAcgccgc                                                                                                        >  1:4572554/1‑150 (MQ=255)
                                                 ttCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTc                                                                                                      >  1:1625927/1‑150 (MQ=255)
                                                  tctacttAATTGATGAAATCACCGCTATCTAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAATAGCATGGACAAGTAATTACCGTGGGTGGCGTATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCa                                                                                                     <  2:3416420/144‑1 (MQ=255)
                                                     ccGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTc                                                                                                  >  2:3166052/1‑150 (MQ=255)
                                                            cGATGCCATCACCGCCAGCTAGCTAATTAACGATATTGAAGCCCTGTTTTAATAAGGAACGAGCATGTACATGGAATTACCGTGGCTGGGGTATAACGCCCAACTGTAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCAtcgtcg                                                                                           <  1:3882666/150‑1 (MQ=255)
                                                            cGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGTACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCAtcgtcg                                                                                           <  2:4670593/150‑1 (MQ=255)
                                                               tGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTg                                                                                        >  1:4385034/1‑150 (MQ=255)
                                                               tGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTg                                                                                        >  1:602260/1‑150 (MQ=255)
                                                                gCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTgg                                                                                       >  2:709409/1‑150 (MQ=255)
                                                                 ccATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGc                                                                                      >  2:2821632/1‑150 (MQ=255)
                                                                    tCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCgg                                                                                   >  2:3522826/1‑150 (MQ=255)
                                                                    tCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCgg                                                                                   >  2:1114928/1‑150 (MQ=255)
                                                                            aGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCa                                                                           >  1:691788/1‑150 (MQ=255)
                                                                              cGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAgt                                                                         >  1:4274736/1‑150 (MQ=255)
                                                                               gAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAgtg                                                                        >  1:1094421/1‑150 (MQ=255)
                                                                                 gCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTc                                                                      >  2:2576000/1‑150 (MQ=255)
                                                                                  cTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTcc                                                                     >  2:4008561/1‑150 (MQ=255)
                                                                                    aaTTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGt                                                                   >  1:4180277/1‑150 (MQ=255)
                                                                                        aaCGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATc                                                               >  1:2254139/1‑150 (MQ=255)
                                                                                           tatatTGAAGCCCTGTTTTATTAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGTAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACt                                                            <  1:4182430/149‑1 (MQ=255)
                                                                                            atatTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTg                                                           <  1:762493/150‑1 (MQ=255)
                                                                                            atatTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTg                                                           >  1:4709552/1‑150 (MQ=255)
                                                                                            atatTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTg                                                           >  2:2996553/1‑150 (MQ=255)
                                                                                             tatgcacgCCGTGTTTTAAAAGGCAAAGACCTTGGACATGCAATTACGGGGGGTGGGGGAACACCCCCACGTGGAACTTAATAAAAAAAACGCCAACACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTgg                                                          <  1:913235/143‑1 (MQ=255)
                                                                                             tatTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTgg                                                          >  1:1649955/1‑150 (MQ=255)
                                                                                              atTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGTAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGa                                                         <  1:2345036/150‑1 (MQ=255)
                                                                                               ttGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACACGGAATTACCGTGGCGGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGaa                                                        <  2:2280895/150‑1 (MQ=255)
                                                                                                tGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAg                                                       >  2:951323/1‑150 (MQ=255)
                                                                                                  aaGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGtc                                                     >  2:2023426/1‑150 (MQ=255)
                                                                                                    gCCCTTTTTGAATAAGGAAAGAGCATGTACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGtctc                                                   <  2:1062800/150‑1 (MQ=255)
                                                                                                      ccTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATACCGCCCAACTGGAACTGAAATATAAAAAAGCCAACACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCAt                                                 <  1:3087397/150‑1 (MQ=255)
                                                                                                       cTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATc                                                >  2:816736/1‑150 (MQ=255)
                                                                                                        tGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATcc                                               >  1:1184651/1‑150 (MQ=255)
                                                                                                        tGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATcc                                               >  1:1184793/1‑150 (MQ=255)
                                                                                                        tGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATcc                                               >  1:1308490/1‑150 (MQ=255)
                                                                                                         gTTTGAATATGGAAAGAGCATGGACTAGGAATTTCCGTTGCTGGCGGATTTGGGCCAACTGGATCTGAAATTTTAAATTGGCATTACGCGGCTCAGTCATCGTCGCTGGCCGGGCGAAACAGTGTCCGTTATCACTGGAAGTCTGATcgt                                              <  2:2304715/150‑3 (MQ=255)
                                                                                                          tttGAATAAGGAACGAGCATGGACAAGGAATTACCGGGGCTGGCGTATAACGCCCAACTGGAACTTAAATATAAAAACGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAg                                             <  1:572009/150‑1 (MQ=255)
                                                                                                          tttGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTTAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAg                                             <  1:3105949/150‑1 (MQ=255)
                                                                                                          tttGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAg                                             <  2:1649955/150‑1 (MQ=255)
                                                                                                            tGAATAAGGAAAGAGCATGTACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGAc                                           <  2:4365122/150‑1 (MQ=255)
                                                                                                            tGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGAc                                           <  2:1365703/150‑1 (MQ=255)
                                                                                                             taaTACGGAACGAGCTGGGCCAGGGATTTCCCGGGGGGGGCGGATACCCCCCAACGGCAACTCAAATATAAACACGCCACCACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACa                                          <  1:3233972/149‑1 (MQ=255)
                                                                                                               aTAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACAtt                                        >  2:3861138/1‑150 (MQ=255)
                                                                                                                 acggAAAGAGCATGGACAAGGAATGCCCGTGGCTGGAGTAAACCGCCCAACTGCAACTCAAATATAAAAAAGCCAACACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTgg                                      <  1:4452918/148‑1 (MQ=255)
                                                                                                                 aaGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTgg                                      <  2:1954398/150‑1 (MQ=255)
                                                                                                                      aaGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGtgat                                 <  1:3512972/150‑1 (MQ=255)
                                                                                                                         agCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGaa                              >  2:445246/1‑150 (MQ=255)
                                                                                                                         agCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGaa                              >  2:4767395/1‑150 (MQ=255)
                                                                                                                          gCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAAt                             <  2:2935255/150‑1 (MQ=255)
                                                                                                                            aTGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTg                           >  1:4675302/1‑150 (MQ=255)
                                                                                                                            aTGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTg                           >  2:1236007/1‑150 (MQ=255)
                                                                                                                              ggACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCt                         >  1:2031900/1‑150 (MQ=255)
                                                                                                                                aCAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTAc                       >  2:1196080/1‑150 (MQ=255)
                                                                                                                                 cAAGTAATCACGGTGGCTGGCGTAAAACCCCCAACTGCAACTTAAATAAAAAAACGCCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACa                      <  1:3304354/150‑1 (MQ=255)
                                                                                                                                   tggATTTACGGTGGGTGTCTTATACCGCCCAACTGTACCTTAAATAAAAAAAAGCCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACaaa                    <  1:1679006/149‑1 (MQ=255)
                                                                                                                                   aGGACTTACCGGGCCTGGCGGAGACCGCCCAACTGGAACTCAAATCTAAAAAACGCAACACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACaaa                    <  2:2522407/150‑1 (MQ=255)
                                                                                                                                     gAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACaaaaa                  >  2:3140125/1‑150 (MQ=255)
                                                                                                                                        ttACCGTGGCTGGAGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACGGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCt               >  1:1067716/1‑150 (MQ=255)
                                                                                                                                         tACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTg              <  1:3059735/150‑1 (MQ=255)
                                                                                                                                             gTGGCTGGCGGATAACGCCCAACTGTAACTTAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGagaa          <  1:3968969/150‑1 (MQ=255)
                                                                                                                                                gctggcGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAgaagaa       <  1:1367062/150‑1 (MQ=255)
                                                                                                                                                  tggcGTATAACGCCCAACTGGAACTTAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaa     <  2:4154242/150‑1 (MQ=255)
                                                                                                                                                  tggcGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaa     <  2:749894/150‑1 (MQ=255)
                                                                                                                                                   ggcGTATAACCCCAAACTGTAACTTAAATAAAAAAAAGCAAACACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaa    <  1:433724/150‑1 (MQ=255)
                                                                                                                                                     cGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGaaaaaaa  >  1:1087190/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAAAGCAGAAAGATATGGCTGGGGGAGTAATTCAACACAGGAAGCGCAGTTCTCCGTAATCGATGCCATCACCGCCAGCGAGCTAATTAACGATATTGAAGCCCTGTTTGAATAAGGAAAGAGCATGGACAAGGAATTACCGTGGCTGGCGGATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAAAAAAA  >  CP009273/2193634‑2193932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A