Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,453,358 G→T 25.6% I197I (ATC→ATA)  fadI ← beta‑ketoacyl‑CoA thiolase, anaerobic, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,453,3580GT25.6% 900.1 / 258.3 379I197I (ATC→ATA) fadIbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit
Reads supporting (aligned to +/- strand):  ref base G (167/114);  new base T (54/44);  total (221/158)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 386 total aligned reads displayed.

TTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTT  >  CP009273/2453209‑2453507
                                                                                                                                                     |                                                                                                                                                     
ttACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTg                                                                                                                                                       <  2:1851138/150‑1 (MQ=255)
       gAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGt                                                                                                                                                >  1:519404/1‑150 (MQ=255)
       gAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGt                                                                                                                                                >  1:3105587/1‑150 (MQ=255)
       gAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGt                                                                                                                                                >  2:3209372/1‑150 (MQ=255)
        aaTATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTa                                                                                                                                               >  1:610862/1‑150 (MQ=255)
            ttgttgTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGtt                                                                                                                                           >  1:1149921/1‑150 (MQ=255)
               ttgtCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTc                                                                                                                                        >  2:4345825/1‑150 (MQ=255)
                  tCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGcc                                                                                                                                     >  2:4463182/1‑150 (MQ=255)
                     tCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCAtt                                                                                                                                  >  1:3732352/1‑150 (MQ=255)
                      cGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCAttt                                                                                                                                 >  2:790479/1‑150 (MQ=255)
                      cGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCAttt                                                                                                                                 <  2:1309795/150‑1 (MQ=255)
                      cGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTACCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCAttt                                                                                                                                 >  2:3887383/1‑150 (MQ=255)
                       gACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTg                                                                                                                                >  2:2430146/1‑150 (MQ=255)
                       gACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTg                                                                                                                                >  1:2233314/1‑150 (MQ=255)
                       gACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTg                                                                                                                                >  1:4138182/1‑150 (MQ=255)
                        aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGc                                                                                                                               <  1:203707/150‑1 (MQ=255)
                          aaGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGctc                                                                                                                             >  2:2980925/1‑150 (MQ=255)
                          aaGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGctc                                                                                                                             >  2:3648113/1‑150 (MQ=255)
                              ggTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCg                                                                                                                         <  1:4315482/150‑1 (MQ=255)
                              ggTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCg                                                                                                                         <  1:930622/150‑1 (MQ=255)
                              ggTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCg                                                                                                                         >  2:5031844/1‑150 (MQ=255)
                               gttgttTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCgg                                                                                                                        <  1:2189726/150‑1 (MQ=255)
                               gttgttTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCgg                                                                                                                        <  1:4655642/150‑1 (MQ=255)
                                 tgttTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGgtg                                                                                                                      >  1:2397874/1‑150 (MQ=255)
                                   tttATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTc                                                                                                                    <  2:507979/150‑1 (MQ=255)
                                     tataAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGc                                                                                                                  >  1:2465121/1‑150 (MQ=255)
                                     tataAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGc                                                                                                                  >  1:3476152/1‑150 (MQ=255)
                                       taAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGccc                                                                                                                >  2:2327681/1‑150 (MQ=255)
                                       taAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGccc                                                                                                                <  1:3944229/150‑1 (MQ=255)
                                         aggaggGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCAt                                                                                                              >  2:3422130/1‑150 (MQ=255)
                                           gaggGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATcc                                                                                                            >  2:3960368/1‑150 (MQ=255)
                                            aggGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTCGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCCTCCg                                                                                                           >  2:4981147/1‑150 (MQ=255)
                                                  aaaGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGcc                                                                                                     <  2:2180698/150‑1 (MQ=255)
                                                  aaaGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGcc                                                                                                     >  1:4944892/1‑150 (MQ=255)
                                                   aaGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCg                                                                                                    <  2:1929501/150‑1 (MQ=255)
                                                   aaGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCg                                                                                                    >  1:1916242/1‑150 (MQ=255)
                                                     ggCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGt                                                                                                  <  2:2902620/150‑1 (MQ=255)
                                                      gCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTa                                                                                                 <  2:3725967/150‑1 (MQ=255)
                                                        aGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGa                                                                                               >  2:1803204/1‑150 (MQ=255)
                                                         gTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTCGaa                                                                                              >  2:12153/1‑150 (MQ=255)
                                                           catcaCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAata                                                                                            <  2:3608781/150‑1 (MQ=255)
                                                            atcaCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAatat                                                                                           <  1:1308029/150‑1 (MQ=255)
                                                            atcaCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAatat                                                                                           >  1:2051820/1‑150 (MQ=255)
                                                             tcaCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATAtt                                                                                          >  2:856577/1‑150 (MQ=255)
                                                              caCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTc                                                                                         >  2:4535567/1‑150 (MQ=255)
                                                                 ctctTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGc                                                                                      >  2:4864310/1‑150 (MQ=255)
                                                                  tcttctTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCt                                                                                     >  1:2538333/1‑150 (MQ=255)
                                                                  tcttctTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCt                                                                                     <  2:549354/150‑1 (MQ=255)
                                                                  tcttctTTGAGATTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGACCGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGACGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTCGAATCTTCTGCt                                                                                     >  2:4553837/1‑150 (MQ=255)
                                                                   cttcttTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTa                                                                                    >  2:2491577/1‑150 (MQ=255)
                                                                   cttcttTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTa                                                                                    <  1:1451470/150‑1 (MQ=255)
                                                                     tcttTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTAcc                                                                                  >  1:1578986/1‑150 (MQ=255)
                                                                     tcttTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTAcc                                                                                  >  2:4796404/1‑150 (MQ=255)
                                                                      cttTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCg                                                                                 >  2:1153650/1‑150 (MQ=255)
                                                                      cttTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCg                                                                                 >  2:5054405/1‑150 (MQ=255)
                                                                        ttGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCa                                                                               <  2:4689278/150‑1 (MQ=255)
                                                                            gTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAggtg                                                                           <  2:3833336/150‑1 (MQ=255)
                                                                              tttCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAggaggt                                                                         >  2:2219033/1‑150 (MQ=255)
                                                                                   gTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACggg                                                                    <  1:3166661/150‑1 (MQ=255)
                                                                                    tCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTCGAATATTCTGCTACCGCAGGTGGTACGGGc                                                                   >  1:2282817/1‑150 (MQ=255)
                                                                                      tGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCAt                                                                 <  1:1083767/150‑1 (MQ=255)
                                                                                      tGACAATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCAt                                                                 >  1:4745436/1‑150 (MQ=255)
                                                                                         ccATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTaa                                                              >  1:137830/1‑150 (MQ=255)
                                                                                         ccATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTaa                                                              <  1:2778173/150‑1 (MQ=255)
                                                                                         ccATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTaa                                                              <  1:3818689/150‑1 (MQ=255)
                                                                                           aTGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGt                                                            >  1:2194690/1‑150 (MQ=255)
                                                                                           aTGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGt                                                            >  1:1800061/1‑150 (MQ=255)
                                                                                            tGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTc                                                           <  1:3214898/150‑1 (MQ=255)
                                                                                             gCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTcg                                                          <  1:4212943/150‑1 (MQ=255)
                                                                                             gCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTcg                                                          >  1:1986456/1‑150 (MQ=255)
                                                                                             gCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTcg                                                          >  1:464092/1‑150 (MQ=255)
                                                                                                 gAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCa                                                      >  2:1515234/1‑150 (MQ=255)
                                                                                                   gCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCaaa                                                    <  2:116924/150‑1 (MQ=255)
                                                                                                    ttttaCGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAAc                                                   <  1:319596/146‑1 (MQ=255)
                                                                                                    cGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCacac                                                   >  2:3110693/1‑150 (MQ=255)
                                                                                                        aCGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAg                                               <  1:1197473/150‑1 (MQ=255)
                                                                                                         cGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAng                                              <  1:4516298/150‑1 (MQ=255)
                                                                                                         cGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAgg                                              >  1:2878132/1‑150 (MQ=255)
                                                                                                            tGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCga                                           <  2:1981159/150‑1 (MQ=255)
                                                                                                            tGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCga                                           <  2:1469292/150‑1 (MQ=255)
                                                                                                             gATGCGAACGGTGCGCTATTGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCgag                                          >  1:2507480/1‑150 (MQ=255)
                                                                                                               tGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCgagag                                        <  1:2120873/150‑1 (MQ=255)
                                                                                                                gCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCgagaga                                       <  2:2743649/150‑1 (MQ=255)
                                                                                                                     cGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGt                                  <  1:4857704/150‑1 (MQ=255)
                                                                                                                       gTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGttt                                <  2:1718834/150‑1 (MQ=255)
                                                                                                                        tGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTCGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTc                               >  2:1052512/1‑150 (MQ=255)
                                                                                                                         gcgcTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCa                              <  2:4324885/150‑1 (MQ=255)
                                                                                                                           gcTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGt                            <  1:2328527/150‑1 (MQ=255)
                                                                                                                           gcTAATGCATCGTGCTGGTCTCGGGTGATGCCGTAGGTTTTCGCCAGTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGt                            <  2:4197569/150‑1 (MQ=255)
                                                                                                                            cTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTc                           <  2:2679288/150‑1 (MQ=255)
                                                                                                                            cTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTc                           >  2:1412767/1‑150 (MQ=255)
                                                                                                                               aTGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCt                        >  2:2967977/1‑150 (MQ=255)
                                                                                                                               aTGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCt                        >  1:3720239/1‑150 (MQ=255)
                                                                                                                                 gCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTgg                      >  1:4001854/1‑150 (MQ=255)
                                                                                                                                     cTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCa                  >  1:1665783/1‑150 (MQ=255)
                                                                                                                                      ttGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCAt                 >  1:325374/1‑150 (MQ=255)
                                                                                                                                          tGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACAGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTa             >  1:2673884/1‑150 (MQ=255)
                                                                                                                                               tcGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGc        <  2:4008167/150‑1 (MQ=255)
                                                                                                                                                cGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCt       >  1:4397553/1‑150 (MQ=255)
                                                                                                                                                     gATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTttgtt  <  2:3136757/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTT  >  CP009273/2453209‑2453507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A