Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,453,358 G→T 29.0% I197I (ATC→ATA)  fadI ← beta‑ketoacyl‑CoA thiolase, anaerobic, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,453,3580GT29.0% 818.6 / inf 399I197I (ATC→ATA) fadIbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit
Reads supporting (aligned to +/- strand):  ref base G (138/145);  new base T (67/49);  total (205/194)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 404 total aligned reads displayed.

TTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTT  >  CP009273/2453209‑2453507
                                                                                                                                                     |                                                                                                                                                     
 tACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGa                                                                                                                                                      <  1:4064445/150‑1 (MQ=255)
  aCCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGAt                                                                                                                                                     <  1:345589/150‑1 (MQ=255)
       gAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGt                                                                                                                                                <  1:1437414/150‑1 (MQ=255)
       gAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGt                                                                                                                                                >  1:4681709/1‑150 (MQ=255)
         atatTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAg                                                                                                                                              <  1:2649363/150‑1 (MQ=255)
          tatTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAgg                                                                                                                                             >  1:818579/1‑150 (MQ=255)
               ttgtCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTc                                                                                                                                        >  1:969787/1‑150 (MQ=255)
                tgtCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCg                                                                                                                                       <  2:1583080/150‑1 (MQ=255)
                tgtCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCg                                                                                                                                       <  2:187489/150‑1 (MQ=255)
                     tCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCAtt                                                                                                                                  >  2:4299214/1‑150 (MQ=255)
                       gACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGTACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTg                                                                                                                                <  2:689375/150‑1 (MQ=255)
                        aCAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGc                                                                                                                               <  1:1525295/150‑1 (MQ=255)
                         cAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGct                                                                                                                              >  1:3363843/1‑150 (MQ=255)
                          aaGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATTCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCAGTAGGTTTTCGCCATTTGctc                                                                                                                             >  2:482141/1‑150 (MQ=255)
                          aaGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGctc                                                                                                                             >  2:4138452/1‑150 (MQ=255)
                          aaGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTCACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATTCCGTAGGTTTTCGCCATTTGctc                                                                                                                             >  1:3153560/1‑150 (MQ=255)
                           aGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGctct                                                                                                                            <  1:2770968/150‑1 (MQ=255)
                           aGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGctct                                                                                                                            >  2:4467030/1‑150 (MQ=255)
                           aGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGctct                                                                                                                            <  2:4036699/150‑1 (MQ=255)
                            gCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTg                                                                                                                           >  2:915174/1‑150 (MQ=255)
                              ggTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCg                                                                                                                         <  2:4706327/150‑1 (MQ=255)
                              ggTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCg                                                                                                                         >  2:2784131/1‑150 (MQ=255)
                              ggTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTAATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCg                                                                                                                         >  1:1563428/1‑150 (MQ=255)
                               gttgttTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCgg                                                                                                                        >  1:3211035/1‑150 (MQ=255)
                                 tgttTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGgtg                                                                                                                      >  2:2363819/1‑150 (MQ=255)
                                   tttATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTc                                                                                                                    <  1:652192/150‑1 (MQ=255)
                                    ttATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCg                                                                                                                   <  1:1836142/150‑1 (MQ=255)
                                    ttATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCg                                                                                                                   <  1:2430940/150‑1 (MQ=255)
                                    ttATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCg                                                                                                                   >  2:3065938/1‑150 (MQ=255)
                                     tataAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGc                                                                                                                  >  1:2162779/1‑150 (MQ=255)
                                      ataAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGcc                                                                                                                 >  1:4545684/1‑150 (MQ=255)
                                      ataAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGcc                                                                                                                 <  1:644078/150‑1 (MQ=255)
                                       taAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGccc                                                                                                                >  2:3949634/1‑150 (MQ=255)
                                        aaGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGGAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCa                                                                                                               >  1:4390747/1‑150 (MQ=255)
                                          ggaggGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATc                                                                                                             >  2:3859765/1‑150 (MQ=255)
                                             gggATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGc                                                                                                          <  1:4642764/150‑1 (MQ=255)
                                               gATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCaa                                                                                                        <  1:3010746/150‑1 (MQ=255)
                                                   aaGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCg                                                                                                    >  2:942382/1‑150 (MQ=255)
                                                    aGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCgg                                                                                                   >  1:3714961/1‑150 (MQ=255)
                                                      gCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTa                                                                                                 <  2:3461888/150‑1 (MQ=255)
                                                       cAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAg                                                                                                >  1:2620169/1‑150 (MQ=255)
                                                          tcatcaCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGATCTGCGGTGTCGACCATCCGCAAGCCGGTAGAat                                                                                             >  1:1646119/1‑150 (MQ=255)
                                                          tcatcaCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAat                                                                                             <  1:3790121/150‑1 (MQ=255)
                                                           catcaCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCTTCCGCAAGCCGGTAGAata                                                                                            <  1:1964418/150‑1 (MQ=255)
                                                           catcaCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAata                                                                                            >  1:2921681/1‑150 (MQ=255)
                                                              caCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTc                                                                                         <  2:2207765/150‑1 (MQ=255)
                                                               aCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCt                                                                                        <  1:599678/150‑1 (MQ=255)
                                                               aCCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCt                                                                                        <  1:249496/150‑1 (MQ=255)
                                                               aCCTCTTCTATCAGATTTCCGTCTGACCATGCCTGACCTGCACGCTGATGCGAACGGTGCGCTAATGCAACATTCTGTACTCGGGTGATGCCGTAGGTTTTCGCCATATGCTCTGAGGTGACGCACATCCGCAAGCCGGTAGAATATTca                                                                                        >  2:1156550/1‑149 (MQ=255)
                                                                ccTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTg                                                                                       <  1:3615509/150‑1 (MQ=255)
                                                                ccTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTg                                                                                       >  2:3590611/1‑150 (MQ=255)
                                                                 ctctTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGc                                                                                      >  1:428198/1‑150 (MQ=255)
                                                                            gTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAggtg                                                                           >  1:2077150/1‑150 (MQ=255)
                                                                             ttttCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAggtgg                                                                          <  2:2571162/150‑1 (MQ=255)
                                                                                  cGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACgg                                                                     >  2:1979449/1‑150 (MQ=255)
                                                                                      tGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCAt                                                                 <  1:4372053/150‑1 (MQ=255)
                                                                                        aCCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTa                                                               >  2:1330336/1‑150 (MQ=255)
                                                                                        aCCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTa                                                               <  2:2920377/150‑1 (MQ=255)
                                                                                         ccATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTaa                                                              >  1:2332278/1‑150 (MQ=255)
                                                                                         ccATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTaa                                                              <  1:2666934/150‑1 (MQ=255)
                                                                                          cATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAg                                                             <  1:1351814/150‑1 (MQ=255)
                                                                                            tGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTc                                                           <  1:3757834/150‑1 (MQ=255)
                                                                                             gCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTcg                                                          <  2:3386557/150‑1 (MQ=255)
                                                                                               cTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTcgcg                                                        >  1:2558603/1‑150 (MQ=255)
                                                                                                   gCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCaaa                                                    <  2:122838/150‑1 (MQ=255)
                                                                                                     ggCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACg                                                  >  1:1268991/1‑150 (MQ=255)
                                                                                                        aCGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAg                                               <  2:4554804/150‑1 (MQ=255)
                                                                                                          gCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGc                                             <  1:3591231/150‑1 (MQ=255)
                                                                                                           cTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCg                                            >  2:356313/1‑150 (MQ=255)
                                                                                                            tGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCga                                           >  2:809901/1‑150 (MQ=255)
                                                                                                               tGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCgagag                                        <  1:4584163/150‑1 (MQ=255)
                                                                                                                 cGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGagaa                                      >  2:2438190/1‑150 (MQ=255)
                                                                                                                  gAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCTGTGTCGCCCATCCTCAAGCCGGTAGAATATTATGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCgagtgtag                                     <  1:4166194/150‑6 (MQ=255)
                                                                                                                   aaCGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGagaaga                                    <  2:4196814/150‑1 (MQ=255)
                                                                                                                   aaCGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGagaaga                                    <  1:3571441/150‑1 (MQ=255)
                                                                                                                    aCGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAagag                                   >  2:4258025/1‑150 (MQ=255)
                                                                                                                     cGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGt                                  <  1:3276017/150‑1 (MQ=255)
                                                                                                                     cGGTGCGCTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGt                                  <  1:1137442/150‑1 (MQ=255)
                                                                                                                      ggTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCACTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGtt                                 >  2:2478881/1‑150 (MQ=255)
                                                                                                                       gTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGttt                                <  1:4238396/150‑1 (MQ=255)
                                                                                                                         gcgcTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGAAAACGCAGGCGAGAGAAGAGTTTCa                              >  1:902754/1‑150 (MQ=255)
                                                                                                                          cgcTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAg                             >  1:586091/1‑150 (MQ=255)
                                                                                                                          cgcTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAg                             <  2:3153580/150‑1 (MQ=255)
                                                                                                                           gcTAATGCATCTTGCTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGt                            >  2:3760865/1‑150 (MQ=255)
                                                                                                                           gcTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGt                            >  1:535722/1‑150 (MQ=255)
                                                                                                                           gcTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGt                            >  1:3843974/1‑150 (MQ=255)
                                                                                                                             tAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCg                          >  1:130939/1‑150 (MQ=255)
                                                                                                                              aaTGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGc                         >  2:3503958/1‑150 (MQ=255)
                                                                                                                                 gCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTgg                      >  1:2392711/1‑150 (MQ=255)
                                                                                                                                   aTCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCt                    <  1:1795547/150‑1 (MQ=255)
                                                                                                                                   aTCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCt                    >  1:727699/1‑150 (MQ=255)
                                                                                                                                     cTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCa                  <  2:1072459/150‑1 (MQ=255)
                                                                                                                                       tGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATg                <  2:1961566/150‑1 (MQ=255)
                                                                                                                                         cTGTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGt              >  2:3287994/1‑150 (MQ=255)
                                                                                                                                           gTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTAc            <  1:3981317/150‑1 (MQ=255)
                                                                                                                                           gTTCTCGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTAc            >  1:1007715/1‑150 (MQ=255)
                                                                                                                                             tctcGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGa          <  2:1491453/150‑1 (MQ=255)
                                                                                                                                              ctcGGGTTATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAg         >  2:1902950/1‑150 (MQ=255)
                                                                                                                                                    tGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTttgt   <  2:621345/150‑1 (MQ=255)
                                                                                                                                                     gATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTttgtt  <  2:4090578/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCGCAAGCCGGTAGAATATTCTGCTACCGCAGGTGGTACGGGCATTAAGTCGCGCAAACGCAGGCGAGAGAAGAGTTTCAGTCGCTGGCTCATGGTACGAGCTTTGTT  >  CP009273/2453209‑2453507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A