Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,424,074 C→T 91.0% D57N (GAC→AAC)  cvpA ← colicin V production membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,424,0740CT91.0% 901.4 / 59.0 279D57N (GAC→AAC) cvpAcolicin V production membrane protein
Reads supporting (aligned to +/- strand):  ref base C (12/13);  new base T (106/148);  total (118/161)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 281 total aligned reads displayed.

ACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTATGG  >  CP009273/2423928‑2424223
                                                                                                                                                  |                                                                                                                                                     
  cGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCaa                                                                                                                                                  >  2:1590324/1‑150 (MQ=255)
  cGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGAGCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCaa                                                                                                                                                  >  2:3847546/1‑150 (MQ=255)
   gAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCACTCCCATTTCGAACCAGTTCGTTTTCaaa                                                                                                                                                 >  2:1089871/1‑150 (MQ=255)
   gAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCaaa                                                                                                                                                 >  2:2008967/1‑150 (MQ=255)
   gAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCaaa                                                                                                                                                 >  2:3365737/1‑150 (MQ=255)
     aaCAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGc                                                                                                                                               <  1:3586823/150‑1 (MQ=255)
        aGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCg                                                                                                                                            >  1:3016355/1‑150 (MQ=255)
             cccAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAAc                                                                                                                                       <  2:1489791/150‑1 (MQ=255)
             cccAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAAc                                                                                                                                       <  1:1919838/150‑1 (MQ=255)
             cccAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAAc                                                                                                                                       >  2:1172533/1‑150 (MQ=255)
              ccAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAAcc                                                                                                                                      <  2:537912/150‑1 (MQ=255)
                aGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAg                                                                                                                                    >  1:243111/1‑150 (MQ=255)
                 gCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGa                                                                                                                                   <  2:301773/150‑1 (MQ=255)
                   aCCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACt                                                                                                                                 <  1:1177563/150‑1 (MQ=255)
                     ccGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGa                                                                                                                               <  2:2598217/150‑1 (MQ=255)
                      cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGAc                                                                                                                              <  1:4534851/150‑1 (MQ=255)
                       gATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACa                                                                                                                             >  1:1304733/1‑150 (MQ=255)
                       gATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACa                                                                                                                             >  1:273480/1‑150 (MQ=255)
                           ggTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTa                                                                                                                         <  1:1453834/150‑1 (MQ=255)
                             tGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCAAGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAAACAGTTCGTTTTCAAAGCACGTAAACAAGACTGAAAGGTacg                                                                                                                       >  2:2498450/1‑148 (MQ=255)
                                  gACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtag                                                                                                                  >  2:3154227/1‑150 (MQ=255)
                                    cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtagta                                                                                                                <  2:2321335/150‑1 (MQ=255)
                                       ccccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATg                                                                                                             <  1:3469703/150‑1 (MQ=255)
                                       ccccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATg                                                                                                             <  1:3469585/150‑1 (MQ=255)
                                               tctcCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGcaa                                                                                                     <  2:3561811/150‑1 (MQ=255)
                                                 tcCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGcaaca                                                                                                   <  2:791561/150‑1 (MQ=255)
                                                  ccaccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGcaacaa                                                                                                  <  1:3254247/150‑1 (MQ=255)
                                                  ccaccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGcaacaa                                                                                                  <  1:1564363/150‑1 (MQ=255)
                                                   caccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaa                                                                                                 >  1:3797851/1‑150 (MQ=255)
                                                   caccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaa                                                                                                 >  1:2488169/1‑150 (MQ=255)
                                                    accaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaag                                                                                                <  1:2140098/150‑1 (MQ=255)
                                                      caACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTCAGTGTAGTAATGACTGGCAACaaagaa                                                                                              >  2:2462478/1‑150 (MQ=255)
                                                      caACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaagaa                                                                                              >  2:160157/1‑150 (MQ=255)
                                                       aaCTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaagaaa                                                                                             <  1:1409957/150‑1 (MQ=255)
                                                        aCTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaagaaag                                                                                            >  1:3522976/1‑150 (MQ=255)
                                                          tGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGca                                                                                          <  2:364061/150‑1 (MQ=255)
                                                              cTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAc                                                                                      >  2:1275794/1‑150 (MQ=255)
                                                              cTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAc                                                                                      >  2:1275633/1‑150 (MQ=255)
                                                                aTCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAccc                                                                                    >  1:3060987/1‑150 (MQ=255)
                                                                 tCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAcccc                                                                                   <  2:1278734/150‑1 (MQ=255)
                                                                  cACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCa                                                                                  <  2:2271687/150‑1 (MQ=255)
                                                                  cACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCa                                                                                  >  1:3239763/1‑150 (MQ=255)
                                                                   aCGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCAt                                                                                 >  1:3438072/1‑150 (MQ=255)
                                                                   aCGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCAt                                                                                 >  1:70590/1‑150 (MQ=255)
                                                                    cGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATg                                                                                >  1:915233/1‑150 (MQ=255)
                                                                    cGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATg                                                                                >  1:3248446/1‑150 (MQ=255)
                                                                     gAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGt                                                                               >  1:3615568/1‑150 (MQ=255)
                                                                     gAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGt                                                                               >  2:3954612/1‑150 (MQ=255)
                                                                         ttCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCAcc                                                                           <  2:3147013/150‑1 (MQ=255)
                                                                         ttCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGAGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCAcc                                                                           <  2:3780024/150‑1 (MQ=255)
                                                                           cACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAg                                                                         <  1:3149034/150‑1 (MQ=255)
                                                                            aCGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGc                                                                        >  2:66762/1‑150 (MQ=255)
                                                                              gATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGa                                                                      >  2:4061305/1‑150 (MQ=255)
                                                                              gATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGa                                                                      <  2:1786993/150‑1 (MQ=255)
                                                                              gATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGa                                                                      >  2:2359621/1‑150 (MQ=255)
                                                                                 aGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATaa                                                                   <  1:2407117/150‑1 (MQ=255)
                                                                                 aGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATaa                                                                   >  2:369725/1‑150 (MQ=255)
                                                                                  gCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGAAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAAc                                                                  <  2:2724636/150‑1 (MQ=255)
                                                                                   cACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACg                                                                 <  1:3034359/150‑1 (MQ=255)
                                                                                     ccAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCt                                                               <  2:3748645/150‑1 (MQ=255)
                                                                                          gATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACg                                                          <  1:3145825/150‑1 (MQ=255)
                                                                                          gATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACg                                                          <  2:514900/150‑1 (MQ=255)
                                                                                            tCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCAGGTCACCAGCGATAACGCTTCACGaa                                                        <  2:920302/150‑1 (MQ=255)
                                                                                                 gTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAg                                                   <  2:1971265/150‑1 (MQ=255)
                                                                                                  tAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGc                                                  <  1:8303/150‑1 (MQ=255)
                                                                                                   aGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGcc                                                 <  1:1993886/150‑1 (MQ=255)
                                                                                                   aGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGcc                                                 <  2:3710134/150‑1 (MQ=255)
                                                                                                   aGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGcc                                                 <  2:1448586/150‑1 (MQ=255)
                                                                                                      gATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCcgcg                                              <  1:100051/150‑1 (MQ=255)
                                                                                                        tAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGa                                            <  2:1754451/150‑1 (MQ=255)
                                                                                                         aaaCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt                                           >  2:1021802/1‑150 (MQ=255)
                                                                                                          aaCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATc                                          <  2:1804358/150‑1 (MQ=255)
                                                                                                            cAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAg                                        >  1:2222860/1‑150 (MQ=255)
                                                                                                            cAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAg                                        >  1:4461792/1‑150 (MQ=255)
                                                                                                               tACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCt                                     <  1:4393198/150‑1 (MQ=255)
                                                                                                                aCCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTa                                    <  2:823406/150‑1 (MQ=255)
                                                                                                                   gcgATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAcc                                 <  2:3206553/150‑1 (MQ=255)
                                                                                                                    cgATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCa                                <  2:1366685/150‑1 (MQ=255)
                                                                                                                     gATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCag                               <  1:177261/150‑1 (MQ=255)
                                                                                                                     gATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCag                               <  1:62313/150‑1 (MQ=255)
                                                                                                                       tGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCCGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagag                             <  2:1293755/150‑1 (MQ=255)
                                                                                                                       tGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagag                             <  2:1293158/150‑1 (MQ=255)
                                                                                                                       tGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagag                             >  2:4189227/1‑150 (MQ=255)
                                                                                                                         gCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGAGGATCAGGCTAACCagagag                           <  1:2812368/150‑1 (MQ=255)
                                                                                                                           aaTCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGa                         <  1:4086163/150‑1 (MQ=255)
                                                                                                                            aTCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaa                        <  1:1717558/150‑1 (MQ=255)
                                                                                                                             tCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaaa                       <  2:2734136/150‑1 (MQ=255)
                                                                                                                                 aTTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAATAGc                   >  2:2822392/1‑150 (MQ=255)
                                                                                                                                 aTTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGc                   <  1:3540703/150‑1 (MQ=255)
                                                                                                                                  tttCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCa                  >  2:2070210/1‑150 (MQ=255)
                                                                                                                                      gAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCa              >  2:2686057/1‑150 (MQ=255)
                                                                                                                                      gAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCa              <  2:1082212/150‑1 (MQ=255)
                                                                                                                                        aCCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCAcc            >  1:523311/1‑150 (MQ=255)
                                                                                                                                        aCCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCAcc            >  1:1111675/1‑150 (MQ=255)
                                                                                                                                          cAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACcgc          >  1:1232678/1‑150 (MQ=255)
                                                                                                                                           aGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACcgcg         >  2:2763635/1‑150 (MQ=255)
                                                                                                                                               cGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCGGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTa     <  2:4453948/150‑1 (MQ=255)
                                                                                                                                               cGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTa     <  2:3625489/150‑1 (MQ=255)
                                                                                                                                                gTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGAAAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTAt    >  2:1996746/1‑150 (MQ=255)
                                                                                                                                                  cTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTATgg  >  1:1682642/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTATGG  >  CP009273/2423928‑2424223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTATGG  >  CP009273/2423926‑2424223
                                                                                                                                                    |                                                                                                                                                     
GCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTT                                                                                                                                                      >  SRR19973076.3151029/1‑150 (MQ=60)
GCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTT                                                                                                                                                      >  SRR19973076.1521993/1‑150 (MQ=60)
GCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTT                                                                                                                                                      >  SRR19973076.484595/1‑150 (MQ=60)
GCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTT                                                                                                                                                      >  SRR19973076.1521597/1‑150 (MQ=60)
   CCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCA                                                                                                                                                   >  SRR19973076.3210089/1‑150 (MQ=60)
   CCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCA                                                                                                                                                   <  SRR19973076.1332606/150‑1 (MQ=60)
       AACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGC                                                                                                                                               <  SRR19973076.3592034/150‑1 (MQ=60)
          AGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCG                                                                                                                                            >  SRR19973076.3020745/1‑150 (MQ=60)
               CCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAAC                                                                                                                                       <  SRR19973076.1922617/150‑1 (MQ=60)
                 CAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCA                                                                                                                                     >  SRR19973076.3151578/1‑150 (MQ=60)
                  AGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAG                                                                                                                                    >  SRR19973076.243525/1‑150 (MQ=60)
                  AGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAG                                                                                                                                    <  SRR19973076.359394/150‑1 (MQ=60)
                     ACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACT                                                                                                                                 <  SRR19973076.1179308/150‑1 (MQ=60)
                        CGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGAC                                                                                                                              <  SRR19973076.2719807/150‑1 (MQ=60)
                        CGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGAC                                                                                                                              <  SRR19973076.4541581/150‑1 (MQ=60)
                         GATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACA                                                                                                                             >  SRR19973076.1306655/1‑150 (MQ=60)
                         GATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACA                                                                                                                             >  SRR19973076.273929/1‑150 (MQ=60)
                          ATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAG                                                                                                                            >  SRR19973076.3464224/1‑150 (MQ=60)
                          ATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAG                                                                                                                            >  SRR19973076.3464331/1‑150 (MQ=60)
                            CGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCGGACTGACAGGT                                                                                                                          >  SRR19973076.907266/1‑150 (MQ=60)
                            CGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGT                                                                                                                          >  SRR19973076.907328/1‑150 (MQ=60)
                             GGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTA                                                                                                                         <  SRR19973076.1455965/150‑1 (MQ=60)
                             GGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTA                                                                                                                         >  SRR19973076.1120575/1‑150 (MQ=60)
                               TGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAG                                                                                                                       >  SRR19973076.639118/1‑150 (MQ=60)
                                  CTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGT                                                                                                                    <  SRR19973076.1709238/150‑1 (MQ=60)
                                  CTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGT                                                                                                                    <  SRR19973076.3970820/150‑1 (MQ=60)
                                  CTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGT                                                                                                                    <  SRR19973076.1709233/150‑1 (MQ=60)
                                     ACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGT                                                                                                                 <  SRR19973076.351686/150‑1 (MQ=60)
                                     ACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGT                                                                                                                 <  SRR19973076.351586/150‑1 (MQ=60)
                                      CAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTA                                                                                                                >  SRR19973076.1831585/1‑150 (MQ=60)
                                       AACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAA                                                                                                               >  SRR19973076.246581/1‑150 (MQ=60)
                                        ACCCCTTTTTCTCCACCAACTGTCCTATCACGAAGTTCACTATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAAT                                                                                                              <  SRR19973076.3252535/150‑1 (MQ=60)
                                         CCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATG                                                                                                             <  SRR19973076.3474607/150‑1 (MQ=60)
                                         CCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATG                                                                                                             <  SRR19973076.3474636/150‑1 (MQ=60)
                                         CCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATG                                                                                                             <  SRR19973076.3474707/150‑1 (MQ=60)
                                         CCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATG                                                                                                             <  SRR19973076.3474725/150‑1 (MQ=60)
                                          CCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGA                                                                                                            <  SRR19973076.4528668/150‑1 (MQ=60)
                                          CCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGA                                                                                                            <  SRR19973076.4528694/150‑1 (MQ=60)
                                             GTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTG                                                                                                         <  SRR19973076.2341138/150‑1 (MQ=60)
                                               TTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTAACAGGTAAGTGTAGTAATGACTGGC                                                                                                       >  SRR19973076.142298/1‑150 (MQ=60)
                                                    CCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAA                                                                                                  <  SRR19973076.1566655/150‑1 (MQ=60)
                                                    CCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAA                                                                                                  <  SRR19973076.3258976/150‑1 (MQ=60)
                                                    CCACCAACTGGCCGATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAA                                                                                                  <  SRR19973076.3258965/150‑1 (MQ=60)
                                                     CACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAA                                                                                                 >  SRR19973076.3803426/1‑150 (MQ=60)
                                                     CACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAA                                                                                                 >  SRR19973076.2491788/1‑150 (MQ=60)
                                                      ACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAG                                                                                                <  SRR19973076.2143204/150‑1 (MQ=60)
                                                      ACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAG                                                                                                <  SRR19973076.4339623/150‑1 (MQ=60)
                                                      ACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAG                                                                                                <  SRR19973076.2143305/150‑1 (MQ=60)
                                                         AACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAA                                                                                             <  SRR19973076.1412035/150‑1 (MQ=60)
                                                         AACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAA                                                                                             >  SRR19973076.4026118/1‑150 (MQ=60)
                                                         AACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGAAATGACTGGCAAAAAAGAAA                                                                                             >  SRR19973076.1768641/1‑150 (MQ=60)
                                                          ACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAG                                                                                            >  SRR19973076.3528085/1‑150 (MQ=60)
                                                          ACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAG                                                                                            >  SRR19973076.3528125/1‑150 (MQ=60)
                                                          ACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAG                                                                                            <  SRR19973076.120526/150‑1 (MQ=60)
                                                               CCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAA                                                                                       >  SRR19973076.2378385/1‑150 (MQ=60)
                                                               CCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAA                                                                                       >  SRR19973076.1230962/1‑150 (MQ=60)
                                                                CTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAC                                                                                      <  SRR19973076.199584/150‑1 (MQ=60)
                                                                 TATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACC                                                                                     >  SRR19973076.2196504/1‑150 (MQ=60)
                                                                  ATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCC                                                                                    >  SRR19973076.3065506/1‑150 (MQ=60)
                                                                  ATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCC                                                                                    >  SRR19973076.3065433/1‑150 (MQ=60)
                                                                    CACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCA                                                                                  >  SRR19973076.3244468/1‑150 (MQ=60)
                                                                     ACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCAT                                                                                 >  SRR19973076.3443054/1‑150 (MQ=60)
                                                                     ACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCAT                                                                                 >  SRR19973076.70742/1‑150 (MQ=60)
                                                                      CGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATG                                                                                >  SRR19973076.3253165/1‑150 (MQ=60)
                                                                      CGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATG                                                                                >  SRR19973076.916584/1‑150 (MQ=60)
                                                                      CGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATG                                                                                >  SRR19973076.3250574/1‑150 (MQ=60)
                                                                       GAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGT                                                                               >  SRR19973076.3620821/1‑150 (MQ=60)
                                                                             CACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAG                                                                         <  SRR19973076.3153561/150‑1 (MQ=60)
                                                                             CACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAG                                                                         <  SRR19973076.3153603/150‑1 (MQ=60)
                                                                                   AGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAA                                                                   <  SRR19973076.2410586/150‑1 (MQ=60)
                                                                                   AGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAA                                                                   <  SRR19973076.102830/150‑1 (MQ=60)
                                                                                    GCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAAC                                                                  >  SRR19973076.1525927/1‑150 (MQ=60)
                                                                                    GCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAAC                                                                  >  SRR19973076.3786627/1‑150 (MQ=60)
                                                                                     CACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACG                                                                 <  SRR19973076.3038771/150‑1 (MQ=60)
                                                                                     CACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACG                                                                 <  SRR19973076.3038591/150‑1 (MQ=60)
                                                                                     CACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACG                                                                 <  SRR19973076.3038577/150‑1 (MQ=60)
                                                                                         AACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTC                                                             <  SRR19973076.3596635/150‑1 (MQ=60)
                                                                                         AACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTC                                                             <  SRR19973076.1324556/150‑1 (MQ=60)
                                                                                            GATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACG                                                          <  SRR19973076.3150389/150‑1 (MQ=60)
                                                                                             ATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGA                                                         <  SRR19973076.3048005/150‑1 (MQ=60)
                                                                                             ATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGA                                                         <  SRR19973076.1927591/150‑1 (MQ=60)
                                                                                               CAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAAC                                                       >  SRR19973076.3180145/1‑150 (MQ=60)
                                                                                               CAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAAC                                                       >  SRR19973076.2016602/1‑150 (MQ=60)
                                                                                               CAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAAC                                                       >  SRR19973076.2459355/1‑150 (MQ=60)
                                                                                                    TAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGC                                                  <  SRR19973076.8258/150‑1 (MQ=60)
                                                                                                    TAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGC                                                  <  SRR19973076.8317/150‑1 (MQ=60)
                                                                                                     TGCGATAAACAGTTCCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCC                                                 <  SRR19973076.4419857/150‑1 (MQ=60)
                                                                                                     AGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCC                                                 <  SRR19973076.1996787/150‑1 (MQ=60)
                                                                                                     AGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCC                                                 <  SRR19973076.4419400/150‑1 (MQ=60)
                                                                                                        GATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCG                                              <  SRR19973076.100246/150‑1 (MQ=60)
                                                                                                              CAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAG                                        >  SRR19973076.2226059/1‑150 (MQ=60)
                                                                                                              CAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAG                                        >  SRR19973076.4468434/1‑150 (MQ=60)
                                                                                                              CAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAG                                        <  SRR19973076.1471878/150‑1 (MQ=60)
                                                                                                              CAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAG                                        <  SRR19973076.3740514/150‑1 (MQ=60)
                                                                                                                 TACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCT                                     <  SRR19973076.4399679/150‑1 (MQ=60)
                                                                                                                    CGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAC                                  <  SRR19973076.2970080/150‑1 (MQ=60)
                                                                                                                      CGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCA                                <  SRR19973076.2510292/150‑1 (MQ=60)
                                                                                                                      CGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCA                                <  SRR19973076.3567877/150‑1 (MQ=60)
                                                                                                                      CGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCA                                <  SRR19973076.3569765/150‑1 (MQ=60)
                                                                                                                       GATGGCAATCCCATTTCGAACCAGTTCGTTTTCACAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAG                               <  SRR19973076.62293/150‑1 (MQ=60)
                                                                                                                       GATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAG                               <  SRR19973076.62452/150‑1 (MQ=60)
                                                                                                                       GATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAG                               <  SRR19973076.177553/150‑1 (MQ=60)
                                                                                                                       GATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAG                               <  SRR19973076.177561/150‑1 (MQ=60)
                                                                                                                       GATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAG                               <  SRR19973076.520343/150‑1 (MQ=60)
                                                                                                                       GATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAG                               <  SRR19973076.520323/150‑1 (MQ=60)
                                                                                                                         TGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAG                             <  SRR19973076.1672174/150‑1 (MQ=60)
                                                                                                                           GCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGAGGATCAGGCTAACCAGAGAG                           <  SRR19973076.2816447/150‑1 (MQ=60)
                                                                                                                           GCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGAGGATCAGGCTAACCAGAGAG                           <  SRR19973076.2816442/150‑1 (MQ=60)
                                                                                                                             AATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGA                         <  SRR19973076.3111746/150‑1 (MQ=60)
                                                                                                                             AATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGA                         <  SRR19973076.4092193/150‑1 (MQ=60)
                                                                                                                              ATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAA                        <  SRR19973076.1720056/150‑1 (MQ=60)
                                                                                                                               TCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAA                       >  SRR19973076.212038/1‑150 (MQ=60)
                                                                                                                               TCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAA                       >  SRR19973076.2748958/1‑150 (MQ=60)
                                                                                                                                 CCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAATCCGCGGATCAGGCTAACCAGAGAGGAAAAA                     <  SRR19973076.1296563/150‑1 (MQ=60)
                                                                                                                                 CCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAA                     <  SRR19973076.2429050/150‑1 (MQ=60)
                                                                                                                                   ATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGC                   <  SRR19973076.1269083/150‑1 (MQ=60)
                                                                                                                                   ATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGC                   <  SRR19973076.3545843/150‑1 (MQ=60)
                                                                                                                                     TTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAA                 <  SRR19973076.329443/150‑1 (MQ=60)
                                                                                                                                     TTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAA                 <  SRR19973076.329444/150‑1 (MQ=60)
                                                                                                                                         AACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCAC             >  SRR19973076.661076/1‑150 (MQ=60)
                                                                                                                                          ACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACC            >  SRR19973076.524145/1‑150 (MQ=60)
                                                                                                                                          ACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACC            >  SRR19973076.1114930/1‑150 (MQ=60)
                                                                                                                                          ACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACC            >  SRR19973076.1113320/1‑150 (MQ=60)
                                                                                                                                          ACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCACGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACC            >  SRR19973076.524147/1‑150 (MQ=60)
                                                                                                                                            CAGTTCGTTTTCAAAGCCCGTAAACCATACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGC          >  SRR19973076.2921691/1‑150 (MQ=60)
                                                                                                                                            CAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGC          >  SRR19973076.1234502/1‑150 (MQ=60)
                                                                                                                                                  GTTTTCAAAGCCCGTAAACCAGACTGACAGTTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACTCTTCACGAACAAAGCCGCGGATCAGTCTAACCAGAGAGGAAAAAGCAATCACCGCGATTAT    <  SRR19973076.2363013/150‑1 (MQ=60)
                                                                                                                                                  GTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTCACCAGAGAGGAAAAAGCAATCACCGCGATTAT    >  SRR19973076.3328649/1‑150 (MQ=60)
                                                                                                                                                  GTTTTAAAAGCCCGTAAACCAGACTGACAGGTAAGTGTTGTAATGACTGTCAACAAAGAAAGCACACAACCATGTCACCAGCGATAACGCTTATCGAACCTAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTAT    <  SRR19973076.2362357/150‑1 (MQ=60)
                                                                                                                                                   TTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTATG   >  SRR19973076.323154/1‑150 (MQ=60)
                                                                                                                                                    CTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTATGG  >  SRR19973076.1685095/1‑150 (MQ=60)
                                                                                                                                                    CTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTATGG  >  SRR19973076.1685101/1‑150 (MQ=60)
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GCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTATGG  >  CP009273/2423926‑2424223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑