Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,424,074 C→T 100% D57N (GAC→AAC)  cvpA ← colicin V production membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,424,0740CT99.5% 669.6 / ‑4.4 201D57N (GAC→AAC) cvpAcolicin V production membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (106/94);  total (107/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 204 total aligned reads displayed.

CCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGAT  >  CP009273/2423929‑2424218
                                                                                                                                                 |                                                                                                                                                
ccGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGtttt                                                                                                                                                <  1:2250052/148‑1 (MQ=255)
     aCAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAg                                                                                                                                           >  1:362265/1‑148 (MQ=255)
     aCAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAg                                                                                                                                           >  1:1263241/1‑148 (MQ=255)
            cccAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTaa                                                                                                                                    <  1:1030732/148‑1 (MQ=255)
              cAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAAc                                                                                                                                  <  1:37440/148‑1 (MQ=255)
               aGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAAcc                                                                                                                                 <  2:1487876/148‑1 (MQ=255)
                 cACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAg                                                                                                                               <  1:560073/148‑1 (MQ=255)
                   cccGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGAc                                                                                                                             >  1:2133655/1‑148 (MQ=255)
                     cGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTg                                                                                                                           <  2:914318/148‑1 (MQ=255)
                       aTCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGAc                                                                                                                         <  2:1000074/148‑1 (MQ=255)
                        tCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACa                                                                                                                        >  1:3286027/1‑148 (MQ=255)
                        tCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACa                                                                                                                        >  2:1206942/1‑148 (MQ=255)
                            tGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTa                                                                                                                    <  1:1595309/148‑1 (MQ=255)
                            tGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTa                                                                                                                    <  1:1846683/148‑1 (MQ=255)
                            tGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTa                                                                                                                    <  2:134242/148‑1 (MQ=255)
                                tGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAgtg                                                                                                                >  1:1182474/1‑148 (MQ=255)
                                 gACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAgtgt                                                                                                               >  1:1441721/1‑148 (MQ=255)
                                 gACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAgtgt                                                                                                               >  2:2728854/1‑148 (MQ=255)
                                   cAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtag                                                                                                             >  2:1356058/1‑148 (MQ=255)
                                     aCCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtagta                                                                                                           >  1:3118288/1‑148 (MQ=255)
                                     aCCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtagta                                                                                                           <  1:1805214/148‑1 (MQ=255)
                                       cccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAAt                                                                                                         >  1:2273761/1‑148 (MQ=255)
                                       cccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAAt                                                                                                         >  1:1402610/1‑148 (MQ=255)
                                        ccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATg                                                                                                        >  2:1464656/1‑148 (MQ=255)
                                        ccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATg                                                                                                        <  2:1661555/148‑1 (MQ=255)
                                         cGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGa                                                                                                       >  2:525288/1‑148 (MQ=255)
                                          gTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGAc                                                                                                      <  1:574795/148‑1 (MQ=255)
                                          gTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGAc                                                                                                      >  2:462925/1‑148 (MQ=255)
                                            tttCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTg                                                                                                    >  2:2565400/1‑148 (MQ=255)
                                             ttCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTgg                                                                                                   <  1:1692170/148‑1 (MQ=255)
                                                    ccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaa                                                                                            <  2:482964/148‑1 (MQ=255)
                                                      aaCTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaaga                                                                                          <  2:202117/148‑1 (MQ=255)
                                                       aCTGGCCTATCAAGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaagaa                                                                                         >  1:2979672/1‑148 (MQ=255)
                                                           gCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGca                                                                                     >  1:501323/1‑148 (MQ=255)
                                                             cTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGcaca                                                                                   <  2:1068847/148‑1 (MQ=255)
                                                             cTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGcaca                                                                                   >  1:238900/1‑148 (MQ=255)
                                                             cTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGcaca                                                                                   >  1:1909278/1‑148 (MQ=255)
                                                              tATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACaa                                                                                  <  1:2796737/148‑1 (MQ=255)
                                                               aTCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAc                                                                                 >  2:1785747/1‑148 (MQ=255)
                                                                  aCGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAcccc                                                                              >  2:2882140/1‑148 (MQ=255)
                                                                   cGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCa                                                                             <  2:1613996/148‑1 (MQ=255)
                                                                     aaGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATg                                                                           <  1:2845820/148‑1 (MQ=255)
                                                                        ttCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCa                                                                        <  1:3172051/148‑1 (MQ=255)
                                                                        ttCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCa                                                                        >  1:452713/1‑148 (MQ=255)
                                                                         tCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCAc                                                                       >  2:2258644/1‑148 (MQ=255)
                                                                             gATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGc                                                                   <  2:3213536/148‑1 (MQ=255)
                                                                                 gCACCACCGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATa                                                               <  2:1205597/148‑1 (MQ=255)
                                                                                 gCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATa                                                               <  2:1806235/148‑1 (MQ=255)
                                                                                 gCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATa                                                               <  2:1605661/148‑1 (MQ=255)
                                                                                  cACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATaa                                                              >  1:7767/1‑148 (MQ=255)
                                                                                  cACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATaa                                                              >  2:795093/1‑148 (MQ=255)
                                                                                    ccAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACg                                                            <  1:2923418/148‑1 (MQ=255)
                                                                                     cAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGc                                                           >  1:651561/1‑148 (MQ=255)
                                                                                     cAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGc                                                           <  1:525288/148‑1 (MQ=255)
                                                                                       aCGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCtt                                                         >  1:1098508/1‑148 (MQ=255)
                                                                                       aCGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCtt                                                         >  1:1311372/1‑148 (MQ=255)
                                                                                       aCGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCtt                                                         >  2:184934/1‑148 (MQ=255)
                                                                                        cGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTc                                                        >  1:1929563/1‑148 (MQ=255)
                                                                                          aTCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCAc                                                      >  1:3141520/1‑148 (MQ=255)
                                                                                           tCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACg                                                     >  1:2254290/1‑148 (MQ=255)
                                                                                            cAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGa                                                    >  2:2674311/1‑148 (MQ=255)
                                                                                             aGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGaa                                                   <  2:608040/148‑1 (MQ=255)
                                                                                                 tAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaaa                                               <  2:3263092/148‑1 (MQ=255)
                                                                                                 tAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaaa                                               <  1:1054677/148‑1 (MQ=255)
                                                                                                  agagATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAg                                              <  1:1804629/145‑1 (MQ=255)
                                                                                                  aGCGATAAACAGTACCGCGATGGCAGTCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAg                                              <  2:3305976/148‑1 (MQ=255)
                                                                                                         aaCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGa                                       <  2:362265/148‑1 (MQ=255)
                                                                                                           cAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATc                                     >  2:2454921/1‑148 (MQ=255)
                                                                                                           cAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATc                                     >  2:916343/1‑148 (MQ=255)
                                                                                                             gTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAg                                   >  2:1410260/1‑148 (MQ=255)
                                                                                                              tACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAgg                                  <  2:1569418/148‑1 (MQ=255)
                                                                                                              tACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAgg                                  >  2:81206/1‑148 (MQ=255)
                                                                                                                ccGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCt                                <  1:3086521/148‑1 (MQ=255)
                                                                                                                   cgATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAGCCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAc                             >  1:1732012/1‑148 (MQ=255)
                                                                                                                   cgATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAc                             >  1:921666/1‑148 (MQ=255)
                                                                                                                    gATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAcc                            <  2:3203927/148‑1 (MQ=255)
                                                                                                                      tGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCag                          >  2:2244619/1‑148 (MQ=255)
                                                                                                                      tGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCag                          <  2:2376698/148‑1 (MQ=255)
                                                                                                                       ggCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCaga                         <  1:3079412/148‑1 (MQ=255)
                                                                                                                       ggCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCaga                         >  2:1805157/1‑148 (MQ=255)
                                                                                                                        gCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagag                        <  2:1182447/148‑1 (MQ=255)
                                                                                                                        gCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagag                        >  1:1965701/1‑148 (MQ=255)
                                                                                                                         cAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagaga                       <  2:2628557/148‑1 (MQ=255)
                                                                                                                           aTCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAgg                     <  2:3103554/148‑1 (MQ=255)
                                                                                                                           aTCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAgg                     >  2:2125260/1‑148 (MQ=255)
                                                                                                                            tCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGa                    <  1:2967444/148‑1 (MQ=255)
                                                                                                                            tCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGa                    >  1:2885882/1‑148 (MQ=255)
                                                                                                                               cATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaaaa                 <  1:949754/148‑1 (MQ=255)
                                                                                                                                 tttCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAg               <  1:1769177/148‑1 (MQ=255)
                                                                                                                                  ttCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGACAAAGc              >  2:313/1‑148 (MQ=255)
                                                                                                                                  ttCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGc              <  1:2725432/148‑1 (MQ=255)
                                                                                                                                  ttCGAACCAGTTCGTTTTCAAAGCCCGAAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGAAAACGCTTCACTAACAAAGAAGAGGATCAGTCTAACCAGAGAGGAAAAAGc              >  1:1167989/1‑148 (MQ=255)
                                                                                                                                    cGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCaa            <  2:1929180/148‑1 (MQ=255)
                                                                                                                                       aCCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCa         >  2:2657587/1‑148 (MQ=255)
                                                                                                                                         cAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGTAAAAAGCAATCAcc       >  1:3145415/1‑148 (MQ=255)
                                                                                                                                         cAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCAcc       >  2:407913/1‑148 (MQ=255)
                                                                                                                                             tCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGa   <  1:632006/148‑1 (MQ=255)
                                                                                                                                             tCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGa   <  1:2632498/148‑1 (MQ=255)
                                                                                                                                             tCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGa   <  1:969395/148‑1 (MQ=255)
                                                                                                                                             tCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGa   <  1:17082/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGAT  >  CP009273/2423929‑2424218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTATGG  >  CP009273/2423925‑2424223
                                                                                                                                                     |                                                                                                                                                     
CGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTT                                                                                                                                                       >  SRR19973070.1052942/1‑150 (MQ=60)
 GCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTT                                                                                                                                                      <  SRR19973070.2756275/150‑1 (MQ=60)
 GCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTT                                                                                                                                                      <  SRR19973070.3141983/150‑1 (MQ=60)
 GCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTT                                                                                                                                                      <  SRR19973070.1490053/150‑1 (MQ=60)
   ACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTC                                                                                                                                                    <  SRR19973070.1866625/150‑1 (MQ=60)
   ACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTC                                                                                                                                                    <  SRR19973070.690521/150‑1 (MQ=60)
    CCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCA                                                                                                                                                   <  SRR19973070.2264939/150‑1 (MQ=60)
    CCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCA                                                                                                                                                   <  SRR19973070.2266046/150‑1 (MQ=60)
    CCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCA                                                                                                                                                   <  SRR19973070.1244007/150‑1 (MQ=60)
       AAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAG                                                                                                                                                >  SRR19973070.1272089/1‑150 (MQ=60)
       AAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAG                                                                                                                                                >  SRR19973070.364916/1‑150 (MQ=60)
                CCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAAC                                                                                                                                       <  SRR19973070.1038026/150‑1 (MQ=60)
                  CAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCA                                                                                                                                     <  SRR19973070.37921/150‑1 (MQ=60)
                  CAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCA                                                                                                                                     <  SRR19973070.37909/150‑1 (MQ=60)
                     CACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGAC                                                                                                                                  >  SRR19973070.2147914/1‑150 (MQ=60)
                     CACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGAC                                                                                                                                  >  SRR19973070.2148035/1‑150 (MQ=60)
                     CACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGAC                                                                                                                                  <  SRR19973070.563764/150‑1 (MQ=60)
                        CCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGA                                                                                                                               <  SRR19973070.1445554/150‑1 (MQ=60)
                          GATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACA                                                                                                                             >  SRR19973070.3307121/1‑150 (MQ=60)
                          GATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACA                                                                                                                             >  SRR19973070.1657535/1‑150 (MQ=60)
                          GATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACA                                                                                                                             >  SRR19973070.521245/1‑150 (MQ=60)
                                TGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAG                                                                                                                       <  SRR19973070.1606459/150‑1 (MQ=60)
                                TGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAG                                                                                                                       <  SRR19973070.1859363/150‑1 (MQ=60)
                                TGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAG                                                                                                                       <  SRR19973070.1859349/150‑1 (MQ=60)
                                  CCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTG                                                                                                                     >  SRR19973070.3123571/1‑150 (MQ=60)
                                  CCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTG                                                                                                                     >  SRR19973070.1190783/1‑150 (MQ=60)
                                  CCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTG                                                                                                                     >  SRR19973070.1190756/1‑150 (MQ=60)
                                   CTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGT                                                                                                                    >  SRR19973070.1451721/1‑150 (MQ=60)
                                   CTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGT                                                                                                                    >  SRR19973070.3103159/1‑150 (MQ=60)
                                    TGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTA                                                                                                                   >  SRR19973070.930556/1‑150 (MQ=60)
                                       CAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTA                                                                                                                >  SRR19973070.3138408/1‑150 (MQ=60)
                                         ACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAAT                                                                                                              >  SRR19973070.2288866/1‑150 (MQ=60)
                                         ACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAAT                                                                                                              <  SRR19973070.1817675/150‑1 (MQ=60)
                                         ACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAAT                                                                                                              <  SRR19973070.1817664/150‑1 (MQ=60)
                                         ACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAAT                                                                                                              >  SRR19973070.2288825/1‑150 (MQ=60)
                                         ACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAAT                                                                                                              >  SRR19973070.1411882/1‑150 (MQ=60)
                                         ACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAAT                                                                                                              >  SRR19973070.1412098/1‑150 (MQ=60)
                                           CCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGA                                                                                                            >  SRR19973070.1046510/1‑150 (MQ=60)
                                             CGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACT                                                                                                          <  SRR19973070.975969/150‑1 (MQ=60)
                                             CGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACT                                                                                                          >  SRR19973070.1152313/1‑150 (MQ=60)
                                             CGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACT                                                                                                          <  SRR19973070.3027996/150‑1 (MQ=60)
                                              GTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTG                                                                                                         <  SRR19973070.577441/150‑1 (MQ=60)
                                              GTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTG                                                                                                         <  SRR19973070.578727/150‑1 (MQ=60)
                                                 TTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCA                                                                                                      <  SRR19973070.1703804/150‑1 (MQ=60)
                                                         CAACTGGCCTATCAAGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAA                                                                                              >  SRR19973070.2998794/1‑150 (MQ=60)
                                                             TGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCA                                                                                          >  SRR19973070.504803/1‑150 (MQ=60)
                                                             TGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCA                                                                                          >  SRR19973070.1942177/1‑150 (MQ=60)
                                                              GGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCAC                                                                                         <  SRR19973070.1715786/150‑1 (MQ=60)
                                                               GCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACA                                                                                        >  SRR19973070.1922178/1‑150 (MQ=60)
                                                               GCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACA                                                                                        >  SRR19973070.240556/1‑150 (MQ=60)
                                                                  TATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACC                                                                                     <  SRR19973070.2816276/150‑1 (MQ=60)
                                                                  TATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACC                                                                                     >  SRR19973070.472930/1‑150 (MQ=60)
                                                                  TATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACC                                                                                     <  SRR19973070.2814781/150‑1 (MQ=60)
                                                                         AAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTC                                                                              <  SRR19973070.2864246/150‑1 (MQ=60)
                                                                         AAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTC                                                                              <  SRR19973070.2864245/150‑1 (MQ=60)
                                                                         AAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTC                                                                              >  SRR19973070.2616629/1‑150 (MQ=60)
                                                                          AGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCA                                                                             >  SRR19973070.455971/1‑150 (MQ=60)
                                                                          AGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCA                                                                             >  SRR19973070.1280861/1‑150 (MQ=60)
                                                                            TTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACC                                                                           <  SRR19973070.3192490/150‑1 (MQ=60)
                                                                            TTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACC                                                                           <  SRR19973070.3192583/150‑1 (MQ=60)
                                                                                CGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCG                                                                       >  SRR19973070.2702116/1‑150 (MQ=60)
                                                                                  ATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGAT                                                                     >  SRR19973070.610580/1‑150 (MQ=60)
                                                                                  ATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGAT                                                                     >  SRR19973070.33364/1‑150 (MQ=60)
                                                                                   TAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATA                                                                    <  SRR19973070.316196/150‑1 (MQ=60)
                                                                                    AGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAA                                                                   >  SRR19973070.7877/1‑150 (MQ=60)
                                                                                       ACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGC                                                                >  SRR19973070.1524444/1‑150 (MQ=60)
                                                                                       ACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGC                                                                >  SRR19973070.654035/1‑150 (MQ=60)
                                                                                       ACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGC                                                                >  SRR19973070.656209/1‑150 (MQ=60)
                                                                                        CCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCT                                                               <  SRR19973070.2940991/150‑1 (MQ=60)
                                                                                        CCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCT                                                               <  SRR19973070.2942295/150‑1 (MQ=60)
                                                                                         CAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTT                                                              >  SRR19973070.1320469/1‑150 (MQ=60)
                                                                                         CAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTT                                                              >  SRR19973070.1106066/1‑150 (MQ=60)
                                                                                         CAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTT                                                              >  SRR19973070.1106068/1‑150 (MQ=60)
                                                                                         CAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTT                                                              <  SRR19973070.528847/150‑1 (MQ=60)
                                                                                          AACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTC                                                             >  SRR19973070.1942562/1‑150 (MQ=60)
                                                                                            CGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCAC                                                           >  SRR19973070.3274149/1‑150 (MQ=60)
                                                                                            CGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCAC                                                           >  SRR19973070.3161821/1‑150 (MQ=60)
                                                                                             GATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACG                                                          >  SRR19973070.2269240/1‑150 (MQ=60)
                                                                                             GATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACG                                                          >  SRR19973070.2957567/1‑150 (MQ=60)
                                                                                                CAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAAC                                                       >  SRR19973070.1946084/1‑150 (MQ=60)
                                                                                                    GTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAG                                                   >  SRR19973070.2459214/1‑150 (MQ=60)
                                                                                                     TAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGC                                                  <  SRR19973070.1062087/150‑1 (MQ=60)
                                                                                                      AGAGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCC                                                 <  SRR19973070.1817085/150‑1 (MQ=60)
                                                                                                         GATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCG                                              <  SRR19973070.913862/150‑1 (MQ=60)
                                                                                                           TAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGA                                            >  SRR19973070.2342838/1‑150 (MQ=60)
                                                                                                              ACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCA                                         <  SRR19973070.445771/150‑1 (MQ=60)
                                                                                                                  TACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCT                                     >  SRR19973070.695915/1‑150 (MQ=60)
                                                                                                                    CCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAA                                   <  SRR19973070.3106374/150‑1 (MQ=60)
                                                                                                                     CGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAGCCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAC                                  >  SRR19973070.1744022/1‑150 (MQ=60)
                                                                                                                     CGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAC                                  >  SRR19973070.928238/1‑150 (MQ=60)
                                                                                                                          TGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAG                             >  SRR19973070.1978970/1‑150 (MQ=60)
                                                                                                                          TGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAG                             >  SRR19973070.1996616/1‑150 (MQ=60)
                                                                                                                           GGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGA                            <  SRR19973070.3099197/150‑1 (MQ=60)
                                                                                                                            GCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAG                           <  SRR19973070.1791613/150‑1 (MQ=60)
                                                                                                                             CAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGG                          <  SRR19973070.185007/150‑1 (MQ=60)
                                                                                                                              AATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGA                         >  SRR19973070.2904564/1‑150 (MQ=60)
                                                                                                                               ATCCCATTTCTAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAA                        >  SRR19973070.1729784/1‑150 (MQ=60)
                                                                                                                                TCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAA                       <  SRR19973070.53610/150‑1 (MQ=60)
                                                                                                                                TCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAA                       <  SRR19973070.2986510/150‑1 (MQ=60)
                                                                                                                                   CATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAG                    <  SRR19973070.956521/150‑1 (MQ=60)
                                                                                                                                    ATTTCGAACCAGTTCGTTTTCAAAGCCCGAAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGAAAACGCTTCACTAACAAAGAAGAGGATCAGTCTAACCAGAGAGGAAAAAGC                   >  SRR19973070.1176166/1‑150 (MQ=60)
                                                                                                                                     TTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCA                  <  SRR19973070.1781383/150‑1 (MQ=60)
                                                                                                                                     TTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCA                  <  SRR19973070.1781420/150‑1 (MQ=60)
                                                                                                                                      TTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAA                 <  SRR19973070.2743013/150‑1 (MQ=60)
                                                                                                                                      TTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAA                 <  SRR19973070.2746449/150‑1 (MQ=60)
                                                                                                                                         GAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGGCAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCA              >  SRR19973070.841626/1‑150 (MQ=60)
                                                                                                                                           ACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGTAAAAAGCAATCACC            >  SRR19973070.3165741/1‑150 (MQ=60)
                                                                                                                                           ACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACC            >  SRR19973070.3165336/1‑150 (MQ=60)
                                                                                                                                                 TCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATT      <  SRR19973070.636490/150‑1 (MQ=60)
                                                                                                                                                 TCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATT      <  SRR19973070.2649663/150‑1 (MQ=60)
                                                                                                                                                 TCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATT      <  SRR19973070.17334/150‑1 (MQ=60)
                                                                                                                                                 TCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATT      <  SRR19973070.976314/150‑1 (MQ=60)
                                                                                                                                                 TCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATT      <  SRR19973070.985321/150‑1 (MQ=60)
                                                                                                                                                  CGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTA     >  SRR19973070.743689/1‑150 (MQ=60)
                                                                                                                                                  CGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTA     <  SRR19973070.2997606/150‑1 (MQ=60)
                                                                                                                                                   GTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTAT    <  SRR19973070.428211/150‑1 (MQ=60)
                                                                                                                                                   GTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTAT    <  SRR19973070.428142/150‑1 (MQ=60)
                                                                                                                                                     TTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTATGG  >  SRR19973070.2208939/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CGCACCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGATTATGG  >  CP009273/2423925‑2424223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑