Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,601,404 C→T 50.9% L377F (CTC→TTC)  hyfF → hydrogenase 4, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,601,4040CT50.9% 1.8 / inf 251L377F (CTC→TTC) hyfFhydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (52/71);  new base T (77/50);  total (129/122)
Fisher's exact test for biased strand distribution p-value = 5.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

Only 100 of 252 total aligned reads displayed.

GAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTA  >  CP009273/2601257‑2601553
                                                                                                                                                   |                                                                                                                                                     
 aGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCa                                                                                                                                                    >  1:2755146/1‑150 (MQ=255)
         aTTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATg                                                                                                                                            >  2:2512472/1‑150 (MQ=255)
           tGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGcc                                                                                                                                          >  1:651659/1‑150 (MQ=255)
            ggCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCa                                                                                                                                         <  1:3820102/150‑1 (MQ=255)
            ggCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCa                                                                                                                                         <  1:1711477/150‑1 (MQ=255)
             gCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCAt                                                                                                                                        <  1:2169770/150‑1 (MQ=255)
                     cTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTAccgcc                                                                                                                                >  2:428936/1‑150 (MQ=255)
                     cTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTAccgcc                                                                                                                                >  1:199165/1‑150 (MQ=255)
                         gAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGc                                                                                                                            >  1:4361084/1‑150 (MQ=255)
                           aTTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTg                                                                                                                          <  1:3601203/150‑1 (MQ=255)
                            tttttGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGt                                                                                                                         >  2:191442/1‑150 (MQ=255)
                             ttttGCCGCGATGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATGTACCGCCGTGCTGtg                                                                                                                        >  2:2367602/1‑149 (MQ=255)
                                  ccGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTggcg                                                                                                                   <  1:1007403/150‑1 (MQ=255)
                                   cgcgCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTggcgg                                                                                                                  <  1:3243187/150‑1 (MQ=255)
                                    gcgcTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTggcggc                                                                                                                 <  1:3539892/150‑1 (MQ=255)
                                     cgcTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGgcggcg                                                                                                                >  1:3116217/1‑150 (MQ=255)
                                     cgcTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGgcggcg                                                                                                                <  2:1041973/150‑1 (MQ=255)
                                        tgctgcACATCTGAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGGTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTg                                                                                                             <  2:3177234/150‑1 (MQ=255)
                                         gctgcACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCACTGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgc                                                                                                            >  2:3005983/1‑150 (MQ=255)
                                          ctgcACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcg                                                                                                           <  1:4516969/150‑1 (MQ=255)
                                           tgcACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgc                                                                                                          >  1:2622181/1‑150 (MQ=255)
                                           tgcACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgc                                                                                                          >  1:3454077/1‑150 (MQ=255)
                                            gcACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGGTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgct                                                                                                         <  1:2468360/150‑1 (MQ=255)
                                             cacaTCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctg                                                                                                        >  2:3900342/1‑150 (MQ=255)
                                              acaTCTTAAACCACAGTCTGGCAAAAACGCTTCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTgcgctgg                                                                                                       <  2:3662206/150‑1 (MQ=255)
                                                     aaaCCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCgctggcgctggc                                                                                                <  1:1233516/150‑1 (MQ=255)
                                                     aaaCCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCgctggcgctggc                                                                                                >  2:3476631/1‑150 (MQ=255)
                                                     aaaCCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCgctggcgctggc                                                                                                >  1:1419939/1‑150 (MQ=255)
                                                         cacaGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAggg                                                                                            <  1:1614380/150‑1 (MQ=255)
                                                           caGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGAt                                                                                          <  2:4163987/150‑1 (MQ=255)
                                                           caGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGAt                                                                                          <  1:2819907/150‑1 (MQ=255)
                                                           caGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGAt                                                                                          <  1:2024951/150‑1 (MQ=255)
                                                              tCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgcc                                                                                       >  1:1680112/1‑150 (MQ=255)
                                                              tCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATgcc                                                                                       >  1:494696/1‑150 (MQ=255)
                                                                   cAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCt                                                                                  <  1:2684511/150‑1 (MQ=255)
                                                                    aaaaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCtt                                                                                 >  2:3091667/1‑150 (MQ=255)
                                                                     aaaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTAGCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTc                                                                                <  2:1903467/150‑1 (MQ=255)
                                                                     aaaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTc                                                                                <  2:4489204/150‑1 (MQ=255)
                                                                     aaaaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTc                                                                                <  1:4009746/150‑1 (MQ=255)
                                                                       aaCGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCaa                                                                              <  1:855331/150‑1 (MQ=255)
                                                                          gctgctGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAt                                                                           <  1:3726941/150‑1 (MQ=255)
                                                                          gctgctGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAt                                                                           >  2:1282756/1‑150 (MQ=255)
                                                                           ctgctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAATATCATGCCGGTTTCCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTccagagt                                                                          >  2:3838366/1‑144 (MQ=255)
                                                                           ctgctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGGTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAtt                                                                          >  2:3838356/1‑150 (MQ=255)
                                                                            tgctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAttt                                                                         <  1:2221066/150‑1 (MQ=255)
                                                                            tgctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAttt                                                                         >  1:336439/1‑150 (MQ=255)
                                                                             gctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAtttt                                                                        >  2:2484365/1‑150 (MQ=255)
                                                                             gctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAtttt                                                                        >  2:3314971/1‑150 (MQ=255)
                                                                              ctgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAttttt                                                                       <  2:411817/150‑1 (MQ=255)
                                                                               tgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTc                                                                      >  2:1669651/1‑150 (MQ=255)
                                                                               tgTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTAAACATTTTTc                                                                      >  2:3605277/1‑150 (MQ=255)
                                                                                    tGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGc                                                                 <  2:1284088/150‑1 (MQ=255)
                                                                                        gTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAAt                                                             >  2:2167271/1‑150 (MQ=255)
                                                                                         ttCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAAtt                                                            <  2:4244207/150‑1 (MQ=255)
                                                                                           ccGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTa                                                          >  2:2010519/1‑150 (MQ=255)
                                                                                           ccGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTa                                                          >  2:2009294/1‑150 (MQ=255)
                                                                                             ggCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTTCTGTTTGCCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATg                                                        >  1:2930379/1‑150 (MQ=255)
                                                                                               cAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGAc                                                      >  1:3306227/1‑150 (MQ=255)
                                                                                                  tGTACTGCTCAAGTACGGCACGCGCTATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCAt                                                   >  2:855556/1‑150 (MQ=255)
                                                                                                   gTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCAtt                                                  <  1:1178755/150‑1 (MQ=255)
                                                                                                   gTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCAtt                                                  <  1:2642840/150‑1 (MQ=255)
                                                                                                     aCTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTAAACATTTTTCTTAGCGAATTTATGACCATTAc                                                <  1:2921898/150‑1 (MQ=255)
                                                                                                          tCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTAccgccg                                           >  2:1586053/1‑150 (MQ=255)
                                                                                                           cAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCgg                                          >  2:3180235/1‑150 (MQ=255)
                                                                                                            aaGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGa                                         >  1:2428587/1‑150 (MQ=255)
                                                                                                             aGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGAc                                        >  2:3011664/1‑150 (MQ=255)
                                                                                                               tACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTg                                      >  2:3971843/1‑150 (MQ=255)
                                                                                                                 cGGCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGc                                    >  2:2426298/1‑150 (MQ=255)
                                                                                                                 cGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGc                                    >  1:4148680/1‑150 (MQ=255)
                                                                                                                   gCACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCAc                                  <  2:1318060/150‑1 (MQ=255)
                                                                                                                   gCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCAc                                  <  1:666884/150‑1 (MQ=255)
                                                                                                                    cACGCGCGATCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACg                                 <  1:1913257/150‑1 (MQ=255)
                                                                                                                    cACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACg                                 >  1:1465804/1‑150 (MQ=255)
                                                                                                                     aCGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGt                                <  2:1582235/150‑1 (MQ=255)
                                                                                                                     aCGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGt                                <  1:1360865/150‑1 (MQ=255)
                                                                                                                      cgcgcgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTa                               >  1:2977049/1‑150 (MQ=255)
                                                                                                                      cgcgcgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACAATTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTa                               <  2:2638413/150‑1 (MQ=255)
                                                                                                                        cgcgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAAt                             <  1:1390588/150‑1 (MQ=255)
                                                                                                                         gcgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGTTGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATc                            <  1:3720087/150‑1 (MQ=255)
                                                                                                                          cgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGAAGCCGCCCATCAACATTTATCTTAGCGAATTTATGACCATTACCGCCGGACAGGCACGAAATCa                           >  1:1838403/1‑150 (MQ=255)
                                                                                                                           gATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCAc                          >  1:949317/1‑150 (MQ=255)
                                                                                                                            aTCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCAcc                         <  2:756205/150‑1 (MQ=255)
                                                                                                                            aTCTCAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCAcc                         <  2:4329065/150‑1 (MQ=255)
                                                                                                                             tctcAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACct                        <  2:3457900/150‑1 (MQ=255)
                                                                                                                             tctcAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACct                        <  2:3459619/150‑1 (MQ=255)
                                                                                                                              ctcAACGTCGTCTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACctg                       >  1:3052986/1‑150 (MQ=255)
                                                                                                                               tcAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACctgc                      >  1:770013/1‑150 (MQ=255)
                                                                                                                                 aaCGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACctgctg                    >  2:1522784/1‑150 (MQ=255)
                                                                                                                                       gtcTGTGGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTCCTGATTATc              >  1:3934889/1‑150 (MQ=255)
                                                                                                                                       gtcTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATc              >  2:2142496/1‑150 (MQ=255)
                                                                                                                                          tgtgGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTc           <  2:4361084/150‑1 (MQ=255)
                                                                                                                                            tgGGATGTTCAATATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGATGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCct         <  1:431175/150‑1 (MQ=255)
                                                                                                                                            tgGGATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCct         <  1:428936/150‑1 (MQ=255)
                                                                                                                                              ggATGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCctgc       >  1:2800136/1‑150 (MQ=255)
                                                                                                                                              ggATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGACTTTATTACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCctgc       >  2:801524/1‑150 (MQ=255)
                                                                                                                                               gATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCctgct      <  2:4469425/150‑1 (MQ=255)
                                                                                                                                               gATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTGGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCctgct      <  2:3280798/150‑1 (MQ=255)
                                                                                                                                                aTGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCctgctg     <  2:2850990/150‑1 (MQ=255)
                                                                                                                                                aTGTTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCctgctg     <  1:3888627/150‑1 (MQ=255)
                                                                                                                                                   cTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTa  <  2:4021624/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GAGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACATCTTAAACCACAGTCTGGCAAAAACGCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTA  >  CP009273/2601257‑2601553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A