Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,601,496 T→A 28.3% F407L (TTT→TTA)  hyfF → hydrogenase 4, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,601,4960TA28.3% 466.9 / 242.0 290F407L (TTT→TTA) hyfFhydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (120/88);  new base A (34/48);  total (154/136)
Fisher's exact test for biased strand distribution p-value = 1.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01

Only 100 of 294 total aligned reads displayed.

CGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCT  >  CP009273/2601349‑2601645
                                                                                                                                                   |                                                                                                                                                     
cGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTAt                                                                                                                                                     <  2:4059922/150‑1 (MQ=255)
 ggCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATg                                                                                                                                                    <  1:1463808/150‑1 (MQ=255)
  gCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGa                                                                                                                                                   <  1:4787336/150‑1 (MQ=255)
       gTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCAtt                                                                                                                                              >  1:4453009/1‑150 (MQ=255)
       gTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCAtt                                                                                                                                              <  2:4991467/150‑1 (MQ=255)
         aCTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTAc                                                                                                                                            >  1:2571729/1‑150 (MQ=255)
         aCTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTAc                                                                                                                                            <  1:1765202/150‑1 (MQ=255)
         aCTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTAc                                                                                                                                            >  2:314465/1‑150 (MQ=255)
         aCTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTAc                                                                                                                                            <  1:3937353/150‑1 (MQ=255)
          cTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCTACTTTTTTCTTAGCGAATTTATGACCATTAcc                                                                                                                                           <  2:3372907/150‑1 (MQ=255)
          cTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTAcc                                                                                                                                           <  2:3433409/150‑1 (MQ=255)
              tCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTAccgccg                                                                                                                                       >  2:2769379/1‑150 (MQ=255)
                 aGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGAc                                                                                                                                    >  2:5003077/1‑150 (MQ=255)
                 aGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACAGCCGGAc                                                                                                                                    >  2:3714595/1‑150 (MQ=255)
                  gTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACt                                                                                                                                   <  1:986718/150‑1 (MQ=255)
                      ggCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCa                                                                                                                               <  2:179380/150‑1 (MQ=255)
                       gCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCAc                                                                                                                              >  2:5257229/1‑150 (MQ=255)
                             gcgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATc                                                                                                                        >  2:1786193/1‑150 (MQ=255)
                             gcgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATc                                                                                                                        <  2:5192636/150‑1 (MQ=255)
                             gcgATCTAAACGTCGTCTGTGTGATGCTCAATTTCTTGCAATTTTCAGCAGTGCTGTTTGGAGGCGGTGCGCTGGCGATGGCAGGTATGCCTCCCTTCAACATTTTTCTTAGCGAATTTATTACCTTTACCGCCGTACTGGCACGTAATc                                                                                                                        <  2:4109711/150‑1 (MQ=255)
                              cgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCa                                                                                                                       >  1:4023298/1‑150 (MQ=255)
                                   tcAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACctgc                                                                                                                  >  1:2644964/1‑150 (MQ=255)
                                    cAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACctgct                                                                                                                 <  1:499703/150‑1 (MQ=255)
                                    cAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACctgct                                                                                                                 <  1:2443355/150‑1 (MQ=255)
                                    cAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACctgct                                                                                                                 >  2:1850828/1‑150 (MQ=255)
                                     aaCGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACctgctg                                                                                                                >  2:2608794/1‑150 (MQ=255)
                                      aCGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGa                                                                                                               <  1:3626423/150‑1 (MQ=255)
                                       cgtcgtCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGAt                                                                                                              >  1:5148730/1‑150 (MQ=255)
                                       cgtcgtCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGAt                                                                                                              <  1:382805/150‑1 (MQ=255)
                                        gtcgtcTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGAtt                                                                                                             <  1:1267706/150‑1 (MQ=255)
                                         tcgtcTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTa                                                                                                            <  2:4532351/150‑1 (MQ=255)
                                           gtcTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATc                                                                                                          >  1:5325954/1‑150 (MQ=255)
                                           gtcTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATc                                                                                                          <  2:4734792/150‑1 (MQ=255)
                                              tgtgGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTc                                                                                                       >  1:4262875/1‑150 (MQ=255)
                                               gtgGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTcc                                                                                                      >  1:2012039/1‑150 (MQ=255)
                                                   gATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCctgct                                                                                                  <  1:316648/150‑1 (MQ=255)
                                                   gATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCctgct                                                                                                  >  1:687555/1‑150 (MQ=255)
                                                     tGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGt                                                                                                >  1:4087564/1‑150 (MQ=255)
                                                       cTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTa                                                                                              <  1:321445/150‑1 (MQ=255)
                                                         cAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCGTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTAtt                                                                                            >  1:987110/1‑150 (MQ=255)
                                                           aaaTCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGc                                                                                          >  2:3719599/1‑150 (MQ=255)
                                                           aaaTCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGc                                                                                          >  2:2146084/1‑150 (MQ=255)
                                                            aaTCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCACTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCt                                                                                         >  2:1233331/1‑150 (MQ=255)
                                                             aTCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTg                                                                                        >  1:2933055/1‑150 (MQ=255)
                                                             aTCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTg                                                                                        >  2:1086632/1‑150 (MQ=255)
                                                             aTCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTg                                                                                        <  2:1921700/150‑1 (MQ=255)
                                                              tCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGt                                                                                       <  1:2566299/150‑1 (MQ=255)
                                                              tCATGCCATTGACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGt                                                                                       <  1:4095064/150‑1 (MQ=255)
                                                                   ccATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACg                                                                                  >  2:835808/1‑150 (MQ=255)
                                                                   ccATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACg                                                                                  <  1:1874626/150‑1 (MQ=255)
                                                                    cATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGc                                                                                 >  1:3330714/1‑150 (MQ=255)
                                                                     aTTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCt                                                                                <  1:349013/150‑1 (MQ=255)
                                                                     aTTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCt                                                                                <  2:799768/150‑1 (MQ=255)
                                                                       ttACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTgg                                                                              <  2:152653/150‑1 (MQ=255)
                                                                       ttACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTgg                                                                              <  2:1759545/150‑1 (MQ=255)
                                                                       ttACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTgg                                                                              <  2:1467787/150‑1 (MQ=255)
                                                                         aCCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTg                                                                            >  1:1190522/1‑150 (MQ=255)
                                                                           cgccgTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCt                                                                          <  2:558566/150‑1 (MQ=255)
                                                                           cgccgTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCt                                                                          >  1:1116054/1‑150 (MQ=255)
                                                                                tGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCgg                                                                     <  2:2035458/150‑1 (MQ=255)
                                                                                    gTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCt                                                                 <  2:4015903/150‑1 (MQ=255)
                                                                                     tttGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTg                                                                <  2:4439924/150‑1 (MQ=255)
                                                                                        ggcggcGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTa                                                             >  1:2197704/1‑150 (MQ=255)
                                                                                             cggTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGAt                                                        <  2:3948212/150‑1 (MQ=255)
                                                                                              ggTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATg                                                       <  1:3446051/150‑1 (MQ=255)
                                                                                                   gctggcgctggcAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTgc                                                  >  1:1048628/1‑150 (MQ=255)
                                                                                                   gctggcgctggcAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTgc                                                  <  2:3996959/150‑1 (MQ=255)
                                                                                                     tggcgctggcAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTgcgc                                                >  2:4496487/1‑150 (MQ=255)
                                                                                                     tggcgctggcAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTgcgc                                                >  2:896557/1‑150 (MQ=255)
                                                                                                     tggcgctggcAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTgcgc                                                >  1:3749645/1‑150 (MQ=255)
                                                                                                         gctggcAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGgt                                            >  2:1636457/1‑150 (MQ=255)
                                                                                                             gcAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTa                                        <  1:878640/150‑1 (MQ=255)
                                                                                                              cAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTaa                                       >  2:731258/1‑150 (MQ=255)
                                                                                                               aGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAAt                                      <  2:4662744/150‑1 (MQ=255)
                                                                                                               aGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAAt                                      >  2:291031/1‑150 (MQ=255)
                                                                                                               aGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAAt                                      >  1:5232576/1‑150 (MQ=255)
                                                                                                                gggATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATg                                     <  2:1213898/150‑1 (MQ=255)
                                                                                                                 ggATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATgg                                    <  1:1000399/150‑1 (MQ=255)
                                                                                                                 ggATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATgg                                    <  1:1471197/150‑1 (MQ=255)
                                                                                                                    tGCCGCCCTTCAACATTTTTCGTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGa                                 <  2:671530/150‑1 (MQ=255)
                                                                                                                     gccgccCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGaa                                <  2:682097/150‑1 (MQ=255)
                                                                                                                       cgccCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAAc                              >  1:3813005/1‑150 (MQ=255)
                                                                                                                       cgccCTTCAACATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAAc                              <  2:3018800/150‑1 (MQ=255)
                                                                                                                            ttCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAAccgccg                         >  2:1393961/1‑150 (MQ=255)
                                                                                                                             tCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACcgccgc                        <  2:2543113/150‑1 (MQ=255)
                                                                                                                               aaCATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAg                      >  2:2381309/1‑150 (MQ=255)
                                                                                                                               aaCATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAg                      >  2:2998974/1‑150 (MQ=255)
                                                                                                                                 cATTTTTCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGc                    >  1:4349550/1‑150 (MQ=255)
                                                                                                                                   tttttCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCg                  >  2:309569/1‑150 (MQ=255)
                                                                                                                                      ttCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTa               >  2:2863253/1‑150 (MQ=255)
                                                                                                                                       tCTTAGCGAATTAATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTaa              >  2:4606032/1‑150 (MQ=255)
                                                                                                                                        cTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAAc             <  2:2187105/150‑1 (MQ=255)
                                                                                                                                         ttAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAAcc            <  1:2265854/150‑1 (MQ=255)
                                                                                                                                         ttAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAAcc            >  1:5197181/1‑150 (MQ=255)
                                                                                                                                          tAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCg           >  2:3230137/1‑150 (MQ=255)
                                                                                                                                          tAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCCGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCg           >  1:2101983/1‑150 (MQ=255)
                                                                                                                                            gCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCggg         <  1:4875033/150‑1 (MQ=255)
                                                                                                                                               aaTTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTg      >  1:2110139/1‑150 (MQ=255)
                                                                                                                                                aTTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGa     <  2:4817573/150‑1 (MQ=255)
                                                                                                                                                   tATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGAtct  <  1:269930/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCT  >  CP009273/2601349‑2601645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A