Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,603,728 A→C 46.3% Q69P (CAG→CCG)  hyfH → hydrogenase 4, Fe‑S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,603,7280AC46.3% 12.7 / inf 231Q69P (CAG→CCG) hyfHhydrogenase 4, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (55/69);  new base C (48/59);  total (103/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

CTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCC  >  CP009273/2603579‑2603877
                                                                                                                                                     |                                                                                                                                                     
cTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCa                                                                                                                                                       <  1:541750/150‑1 (MQ=255)
    gcgcCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGctc                                                                                                                                                   >  1:1039453/1‑150 (MQ=255)
     cgcCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGctct                                                                                                                                                  <  2:1070824/150‑1 (MQ=255)
     cgcCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGctct                                                                                                                                                  >  1:1049375/1‑150 (MQ=255)
     cgcCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCATGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGctct                                                                                                                                                  <  1:3493332/150‑1 (MQ=255)
       ccACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTAt                                                                                                                                                <  1:1318327/150‑1 (MQ=255)
        cACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATc                                                                                                                                               >  2:1256183/1‑150 (MQ=255)
          cTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTg                                                                                                                                             <  2:2193387/150‑1 (MQ=255)
           tGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTgg                                                                                                                                            <  2:3915878/150‑1 (MQ=255)
               ggTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTCTCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCg                                                                                                                                        >  2:1880609/1‑150 (MQ=255)
                gTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGt                                                                                                                                       <  2:2962333/150‑1 (MQ=255)
                 tCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGtt                                                                                                                                      <  2:3570023/150‑1 (MQ=255)
                 tCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGtt                                                                                                                                      <  1:2044667/150‑1 (MQ=255)
                  cAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTg                                                                                                                                     >  2:3290166/1‑150 (MQ=255)
                   aGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGt                                                                                                                                    >  1:3466957/1‑150 (MQ=255)
                   aGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGt                                                                                                                                    <  1:4025677/150‑1 (MQ=255)
                       cTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTAttt                                                                                                                                >  2:2720539/1‑150 (MQ=255)
                             ttCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCg                                                                                                                          <  2:2102587/150‑1 (MQ=255)
                              tCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCgg                                                                                                                         >  1:692978/1‑150 (MQ=255)
                                gcgGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGAc                                                                                                                       <  1:1789198/150‑1 (MQ=255)
                                  ggAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGt                                                                                                                     >  2:1327584/1‑150 (MQ=255)
                                  ggAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGt                                                                                                                     >  2:3156525/1‑150 (MQ=255)
                                   gAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACggt                                                                                                                    >  2:3172448/1‑148 (MQ=255)
                                     aaaCCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTtgt                                                                                                                  >  1:2499772/1‑150 (MQ=255)
                                     aaaCCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTtgt                                                                                                                  >  1:2968562/1‑150 (MQ=255)
                                       aCCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTga                                                                                                                <  1:2462924/150‑1 (MQ=255)
                                       aCCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTga                                                                                                                <  1:1798422/150‑1 (MQ=255)
                                         cGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTgaag                                                                                                              <  2:2536876/150‑1 (MQ=255)
                                         cGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTgaag                                                                                                              >  2:1484214/1‑150 (MQ=255)
                                         cGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTgaag                                                                                                              <  2:3355685/150‑1 (MQ=255)
                                            aCCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGaagaag                                                                                                           <  2:1754345/150‑1 (MQ=255)
                                              cTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAgtg                                                                                                         <  2:1216097/150‑1 (MQ=255)
                                               tGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAgtgt                                                                                                        <  1:3741445/150‑1 (MQ=255)
                                               tGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAgtgt                                                                                                        <  1:3283692/150‑1 (MQ=255)
                                                          cAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCaga                                                                                             >  1:873993/1‑150 (MQ=255)
                                                            aTGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGc                                                                                           >  1:3915354/1‑150 (MQ=255)
                                                             tGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGcc                                                                                          <  2:2737978/150‑1 (MQ=255)
                                                                 ttGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATcc                                                                                      <  1:2312727/150‑1 (MQ=255)
                                                                 ttGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATcc                                                                                      <  1:2313372/150‑1 (MQ=255)
                                                                  tGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCa                                                                                     >  2:826106/1‑150 (MQ=255)
                                                                     cTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCt                                                                                  <  2:1856978/150‑1 (MQ=255)
                                                                     cTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCt                                                                                  <  1:4145472/150‑1 (MQ=255)
                                                                         ggTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTAcc                                                                              >  2:3927706/1‑150 (MQ=255)
                                                                         ggTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTAcc                                                                              <  1:2696119/150‑1 (MQ=255)
                                                                         ggTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTAcc                                                                              >  2:3809714/1‑150 (MQ=255)
                                                                          gTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCa                                                                             <  2:1606559/150‑1 (MQ=255)
                                                                               tGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAAc                                                                        >  1:1551167/1‑150 (MQ=255)
                                                                               tGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAAc                                                                        <  2:3478481/150‑1 (MQ=255)
                                                                                gcgcCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACt                                                                       <  2:742542/150‑1 (MQ=255)
                                                                                 cgcCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACtt                                                                      <  1:1843110/150‑1 (MQ=255)
                                                                                  gcCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACttt                                                                     <  2:719982/150‑1 (MQ=255)
                                                                                     tgtgCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGaa                                                                  >  2:3264324/1‑150 (MQ=255)
                                                                                     tgtgCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGaa                                                                  >  2:8699/1‑150 (MQ=255)
                                                                                      gtgCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAAc                                                                 >  1:3421270/1‑150 (MQ=255)
                                                                                      gtgCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAAc                                                                 <  2:3981567/150‑1 (MQ=255)
                                                                                       tgCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACt                                                                <  2:524545/150‑1 (MQ=255)
                                                                                        gCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTg                                                               <  2:4147386/150‑1 (MQ=255)
                                                                                         cTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGa                                                              <  1:3134073/150‑1 (MQ=255)
                                                                                         cTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGa                                                              <  2:1882530/150‑1 (MQ=255)
                                                                                           tGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGAcc                                                            <  2:314530/150‑1 (MQ=255)
                                                                                             tCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGt                                                          >  2:2904643/1‑150 (MQ=255)
                                                                                              ccGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTc                                                         >  2:808888/1‑150 (MQ=255)
                                                                                                ggCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCAc                                                       <  1:3064399/150‑1 (MQ=255)
                                                                                                  cAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCa                                                     <  1:1166728/150‑1 (MQ=255)
                                                                                                    aaTGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGAATTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACAAATAACTTTGAACTGACCGTCACCAAt                                                   <  1:1441985/150‑1 (MQ=255)
                                                                                                      tGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATaa                                                 <  1:1179780/150‑1 (MQ=255)
                                                                                                        cgcTGACTATCCAGACCGACGACCAGAAAAATTAGCGCACCTGGCCGCTCTATCTTGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGACCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAg                                               <  1:39280/150‑1 (MQ=255)
                                                                                                         gcTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGc                                              <  1:2485220/150‑1 (MQ=255)
                                                                                                         gcTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGc                                              <  1:1605721/150‑1 (MQ=255)
                                                                                                         gcTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGc                                              <  1:2895889/150‑1 (MQ=255)
                                                                                                           tGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCg                                            >  1:993334/1‑150 (MQ=255)
                                                                                                           tGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCg                                            >  1:993291/1‑150 (MQ=255)
                                                                                                           tGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCg                                            <  2:3679192/150‑1 (MQ=255)
                                                                                                             aCTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGAt                                          >  2:934608/1‑150 (MQ=255)
                                                                                                              cTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGAtc                                         >  2:2024734/1‑150 (MQ=255)
                                                                                                               tATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGAtct                                        <  1:2849889/150‑1 (MQ=255)
                                                                                                                   cAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCtat                                    <  1:790889/150‑1 (MQ=255)
                                                                                                                      aCCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATAcc                                 >  2:1122743/1‑150 (MQ=255)
                                                                                                                       ccGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCAGATCTCTATAccc                                <  2:3458505/150‑1 (MQ=255)
                                                                                                                        cgacgaCCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCCATAAAGCCGATCTCTATACCcg                               >  1:2208666/1‑150 (MQ=255)
                                                                                                                        cgacgaCCAGCAAAATTCGCGCACCTGGCCGCTAAATCTGGGGAGTTGTAATTACATCGGACGTTGTGAAGAAGTTTTCCCGACCAGAGCCAACAAGCTTACCAAAAACTTTGAACTGACCGTCACCAAAAAAACCGATCACTATACCcg                               >  1:712046/1‑150 (MQ=255)
                                                                                                                          acgacCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCcgcg                             <  2:1097110/150‑1 (MQ=255)
                                                                                                                          acgacCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCcgcg                             >  1:2591496/1‑150 (MQ=255)
                                                                                                                           cgacCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCcgcgc                            >  1:1360781/1‑150 (MQ=255)
                                                                                                                            gacCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCcgcgcg                           >  2:355756/1‑150 (MQ=255)
                                                                                                                                  cAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTc                     >  2:552057/1‑150 (MQ=255)
                                                                                                                                     aaTTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCAt                  >  1:3162803/1‑150 (MQ=255)
                                                                                                                                      aTTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATc                 <  1:420953/150‑1 (MQ=255)
                                                                                                                                       ttCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCt                <  2:2938762/150‑1 (MQ=255)
                                                                                                                                         cgcgCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTAc              <  1:137619/150‑1 (MQ=255)
                                                                                                                                         cgcgCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTAc              <  2:3713217/150‑1 (MQ=255)
                                                                                                                                             cACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACg          <  1:2459248/150‑1 (MQ=255)
                                                                                                                                             cACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACg          <  1:2459139/150‑1 (MQ=255)
                                                                                                                                             cACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACg          >  1:2334278/1‑150 (MQ=255)
                                                                                                                                              aCCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAAGAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGt         <  2:346591/150‑1 (MQ=255)
                                                                                                                                               ccTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATCACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGtt        <  2:2688505/150‑1 (MQ=255)
                                                                                                                                               ccTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGtt        <  2:1936649/150‑1 (MQ=255)
                                                                                                                                               ccTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGtt        >  2:2004188/1‑150 (MQ=255)
                                                                                                                                                  ggCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCa     >  2:3614882/1‑150 (MQ=255)
                                                                                                                                                     aGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGcc  >  1:2113416/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGCC  >  CP009273/2603579‑2603877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A