Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,603,728 A→C 45.1% Q69P (CAG→CCG)  hyfH → hydrogenase 4, Fe‑S subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,603,7280AC45.1% 14.7 / inf 262Q69P (CAG→CCG) hyfHhydrogenase 4, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (67/77);  new base C (52/66);  total (119/143)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 262 total aligned reads displayed.

CGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGC  >  CP009273/2603582‑2603876
                                                                                                                                                  |                                                                                                                                                    
cgcgCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGct                                                                                                                                                   <  1:1514778/150‑1 (MQ=255)
 gcgcCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGctc                                                                                                                                                  <  2:4231450/150‑1 (MQ=255)
  cgcCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGctct                                                                                                                                                 >  1:1880512/1‑150 (MQ=255)
      aCTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCt                                                                                                                                             >  1:808129/1‑150 (MQ=255)
       cTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTAGTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTg                                                                                                                                            <  1:1467084/150‑1 (MQ=255)
        tGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTgg                                                                                                                                           <  2:1965132/150‑1 (MQ=255)
        tGGAGGTCAGCCCAGGCATACGCGGAAAACCGGACCTGATGCCCAGCCAATGTATAGCCTGCGGAGCCAGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTgg                                                                                                                                           >  2:1523202/1‑150 (MQ=255)
         ggAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTggg                                                                                                                                          <  1:3624652/150‑1 (MQ=255)
          gAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTgggg                                                                                                                                         <  1:1624754/150‑1 (MQ=255)
          gAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTgggg                                                                                                                                         <  2:2377264/150‑1 (MQ=255)
            ggTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCg                                                                                                                                       <  2:465166/150‑1 (MQ=255)
              tCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGtt                                                                                                                                     <  1:4548279/150‑1 (MQ=255)
               cAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTg                                                                                                                                    <  2:2070129/150‑1 (MQ=255)
               cAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTg                                                                                                                                    <  2:1615505/150‑1 (MQ=255)
                  cccTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTAt                                                                                                                                 <  2:577036/150‑1 (MQ=255)
                  cccTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTAt                                                                                                                                 <  2:576896/150‑1 (MQ=255)
                      ggCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTAc                                                                                                                             >  2:2719071/1‑150 (MQ=255)
                       gCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACt                                                                                                                            <  1:34839/150‑1 (MQ=255)
                             gcgGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGAc                                                                                                                      >  1:195594/1‑150 (MQ=255)
                              cgGAAAACCGGACCTGATGCCCAGCCACTGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACg                                                                                                                     <  2:4292928/150‑1 (MQ=255)
                              cgGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACg                                                                                                                     >  1:1122323/1‑150 (MQ=255)
                                gAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGtt                                                                                                                   >  1:4303758/1‑150 (MQ=255)
                                  aaaCCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTtgt                                                                                                                 >  1:3209119/1‑150 (MQ=255)
                                   aaCCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTtgtg                                                                                                                <  2:869453/150‑1 (MQ=255)
                                   aaCCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTtgtg                                                                                                                >  1:1585611/1‑150 (MQ=255)
                                    aCCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTga                                                                                                               <  2:2262896/150‑1 (MQ=255)
                                      cGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTgaag                                                                                                             >  2:207275/1‑150 (MQ=255)
                                      cGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTgaag                                                                                                             <  2:3541317/150‑1 (MQ=255)
                                       ggACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTgaaga                                                                                                            <  2:1450660/150‑1 (MQ=255)
                                          ccTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAgt                                                                                                         >  1:2776108/1‑150 (MQ=255)
                                          ccTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAgt                                                                                                         >  2:1696573/1‑150 (MQ=255)
                                           cTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAgtg                                                                                                        <  2:2455874/150‑1 (MQ=255)
                                             gATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGtgtg                                                                                                      <  2:2532840/150‑1 (MQ=255)
                                              aTGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGc                                                                                                     >  2:3259490/1‑150 (MQ=255)
                                               tGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGcc                                                                                                    >  2:2125672/1‑150 (MQ=255)
                                                   cAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGAc                                                                                                >  2:1795430/1‑150 (MQ=255)
                                                        aaTGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCagag                                                                                           >  2:3977244/1‑150 (MQ=255)
                                                           gTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCa                                                                                        <  1:3089317/150‑1 (MQ=255)
                                                           gTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCa                                                                                        <  1:2582161/150‑1 (MQ=255)
                                                            tATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCAt                                                                                       <  1:3192256/150‑1 (MQ=255)
                                                            tATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCAt                                                                                       >  2:4513665/1‑150 (MQ=255)
                                                             aTTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATc                                                                                      >  2:1002993/1‑150 (MQ=255)
                                                              ttGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATcc                                                                                     >  2:3060120/1‑150 (MQ=255)
                                                                  cTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATACAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCt                                                                                 <  1:2751360/150‑1 (MQ=255)
                                                                   tGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCtt                                                                                <  1:1558921/150‑1 (MQ=255)
                                                                   tGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCtt                                                                                <  1:2127324/150‑1 (MQ=255)
                                                                       gTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCa                                                                            >  1:4500618/1‑150 (MQ=255)
                                                                        tGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCaa                                                                           >  2:1347782/1‑150 (MQ=255)
                                                                            tGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAAc                                                                       <  1:2459859/150‑1 (MQ=255)
                                                                              cgcCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACtt                                                                     >  2:401147/1‑150 (MQ=255)
                                                                                ccTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTg                                                                   <  2:4134138/150‑1 (MQ=255)
                                                                                 cTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGa                                                                  >  2:810023/1‑150 (MQ=255)
                                                                                  tgtgCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGaa                                                                 <  1:4067821/150‑1 (MQ=255)
                                                                                  tgtgCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGaa                                                                 <  2:220377/150‑1 (MQ=255)
                                                                                  tgtgCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGaa                                                                 <  2:4185223/150‑1 (MQ=255)
                                                                                     gCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTg                                                              <  1:2872738/150‑1 (MQ=255)
                                                                                         gTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGGTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCg                                                          >  2:627872/1‑150 (MQ=255)
                                                                                            cGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCa                                                       <  2:3128514/150‑1 (MQ=255)
                                                                                            cGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCa                                                       >  1:4373765/1‑150 (MQ=255)
                                                                                             ggCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCAc                                                      >  1:1414575/1‑150 (MQ=255)
                                                                                               cAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCa                                                    <  2:2038559/150‑1 (MQ=255)
                                                                                                 aaTGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAAt                                                  <  2:746462/150‑1 (MQ=255)
                                                                                                  aTGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATa                                                 <  1:192628/150‑1 (MQ=255)
                                                                                                  aTGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATa                                                 <  2:4040264/150‑1 (MQ=255)
                                                                                                  aTGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATa                                                 <  1:310309/150‑1 (MQ=255)
                                                                                                   tGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATaa                                                >  2:1805047/1‑150 (MQ=255)
                                                                                                   tGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATaa                                                <  2:3185140/150‑1 (MQ=255)
                                                                                                    gcgcTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATaaa                                               <  1:753197/150‑1 (MQ=255)
                                                                                                       cTGACTATCCAGACCGACGACCATCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGcc                                            <  2:3005044/150‑1 (MQ=255)
                                                                                                       cTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGcc                                            <  1:1713152/150‑1 (MQ=255)
                                                                                                       cTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGcc                                            >  2:4313835/1‑150 (MQ=255)
                                                                                                        tGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCg                                           >  1:2062958/1‑150 (MQ=255)
                                                                                                          aCTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGAt                                         <  1:3324913/150‑1 (MQ=255)
                                                                                                            tATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGAtct                                       >  2:698085/1‑150 (MQ=255)
                                                                                                            tATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGAtct                                       >  2:2407386/1‑150 (MQ=255)
                                                                                                            tATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGAtct                                       <  1:1200849/150‑1 (MQ=255)
                                                                                                             aTCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGAtctc                                      >  1:3432696/1‑150 (MQ=255)
                                                                                                             aTCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGAtctc                                      >  2:24722/1‑150 (MQ=255)
                                                                                                              tCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATctct                                     >  2:35500/1‑150 (MQ=255)
                                                                                                                 aGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCtata                                  >  2:1490697/1‑150 (MQ=255)
                                                                                                                    ccGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGCCCGTCACCAATAAAGCCGATCTCTATAccc                               <  1:3886577/150‑1 (MQ=255)
                                                                                                                      gacgacCAGCAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCcgc                             >  1:2042185/1‑150 (MQ=255)
                                                                                                                        cgacCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCcgcgc                           >  1:2470738/1‑150 (MQ=255)
                                                                                                                         gacCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCcgcgcg                          <  2:3106945/150‑1 (MQ=255)
                                                                                                                         gacCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCcgcgcg                          >  1:110067/1‑150 (MQ=255)
                                                                                                                            cAGCAATATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGAACAGAGCCATCCAGCTTACCTATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACGAGCGCGagg                       <  2:1564610/150‑3 (MQ=255)
                                                                                                                             aGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGt                      >  1:3555831/1‑150 (MQ=255)
                                                                                                                               cAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTc                    >  1:1005211/1‑150 (MQ=255)
                                                                                                                               cAAAATTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTc                    >  2:681588/1‑150 (MQ=255)
                                                                                                                                  aaTTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCAt                 >  1:4149740/1‑150 (MQ=255)
                                                                                                                                   aTTCGCGCACCTGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATc                <  2:3374164/150‑1 (MQ=255)
                                                                                                                                   aTTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATCACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATc                <  2:363254/150‑1 (MQ=255)
                                                                                                                                    ttCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCt               <  2:4477425/150‑1 (MQ=255)
                                                                                                                                    ttCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCt               <  2:1122323/150‑1 (MQ=255)
                                                                                                                                      cgcgCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTAc             >  2:45160/1‑150 (MQ=255)
                                                                                                                                        cgcACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACaa           <  1:873120/150‑1 (MQ=255)
                                                                                                                                         gcACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAAc          <  1:4035552/150‑1 (MQ=255)
                                                                                                                                              tGGCCGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGc     >  1:53343/1‑150 (MQ=255)
                                                                                                                                              tGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGc     >  1:1056633/1‑150 (MQ=255)
                                                                                                                                                gCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAg   <  2:3495592/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGTTGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCAATAAAGCCGATCTCTATACCCGCGCGACGTTCCATCTACAACGTTGCAGC  >  CP009273/2603582‑2603876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A