Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F202 I0 R1	1397	275.7	9089454	95.2%	8653160	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,016,372	C→A	10.5%	F566L (TTC→TTA)	xdhD →	putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,016,372	0	C	A	10.5%	949.7 / 58.1	272	F566L (TTC→TTA)	xdhD	putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
Reads supporting (aligned to +/- strand): ref base C (122/121); new base A (11/18); total (133/139)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 274 total aligned reads displayed.

AACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGTTCCTTCCGC  >  CP009273/3016223‑3016521

aaCCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTc                                                                                                                                                       <  2:1505006/150‑1 (MQ=255)

   cactcactCACCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCt                                                                                                                                                    <  1:2940510/150‑1 (MQ=255)

    actcactcACCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTa                                                                                                                                                   >  2:3197622/1‑150 (MQ=255)

    actcactcACCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTa                                                                                                                                                   >  2:4496642/1‑150 (MQ=255)

       cactcacCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTCCTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCa                                                                                                                                                >  1:1395703/1‑150 (MQ=255)

       cactcacCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCa                                                                                                                                                <  2:1709150/150‑1 (MQ=255)

            accgtaccgtGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATg                                                                                                                                           <  1:2178824/150‑1 (MQ=255)

               gtaccgtGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCa                                                                                                                                        <  1:2256597/150‑1 (MQ=255)

                taccgtGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCAt                                                                                                                                       <  2:279172/150‑1 (MQ=255)

                 accgtGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCAtt                                                                                                                                      >  2:1759171/1‑150 (MQ=255)

                  ccgtGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTa                                                                                                                                     <  1:2812388/150‑1 (MQ=255)

                  ccgtGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTa                                                                                                                                     >  2:815888/1‑150 (MQ=255)

                    gtgtAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTACCTTAGCTATCACCATGGCATTAGc                                                                                                                                   >  2:3716214/1‑150 (MQ=255)

                     tgtAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCg                                                                                                                                  <  1:1546493/150‑1 (MQ=255)

                        aaCGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGaa                                                                                                                               >  1:3632700/1‑150 (MQ=255)

                         aCGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAAc                                                                                                                              <  1:2395587/150‑1 (MQ=255)

                          cggtgcGGCGCTGTAGCTGACGTTATATCCGTGAGATATCGTCTATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAATTGGTGCTTATCAGGGTTTTGGCGCACCTAAAGGTAACTTAGATTTCACCTTGGCATTAGCGGAACt                                                                                                                             <  2:3505309/145‑1 (MQ=255)

                          cGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACt                                                                                                                             <  1:188599/150‑1 (MQ=255)

                          cGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACt                                                                                                                             >  2:3007637/1‑150 (MQ=255)

                           ggACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTg                                                                                                                            <  2:2639127/150‑1 (MQ=255)

                                ggCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGa                                                                                                                       >  2:4170677/1‑150 (MQ=255)

                                ggCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGa                                                                                                                       <  1:4496642/150‑1 (MQ=255)

                                  cgcTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAAc                                                                                                                     <  2:3924944/150‑1 (MQ=255)

                                  cgcTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAAc                                                                                                                     <  2:4061808/150‑1 (MQ=255)

                                      gTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGtt                                                                                                                 <  2:4345536/150‑1 (MQ=255)

                                        cGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTAc                                                                                                               <  1:4270226/150‑1 (MQ=255)

                                        cGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTAc                                                                                                               <  2:1631892/150‑1 (MQ=255)

                                        cGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTAc                                                                                                               >  1:3179207/1‑150 (MQ=255)

                                          cTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAg                                                                                                             >  2:1805123/1‑150 (MQ=255)

                                            gCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGag                                                                                                           <  1:1224934/150‑2 (MQ=255)

                                            gCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGAt                                                                                                           <  2:4265404/150‑1 (MQ=255)

                                              cGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGCTCg                                                                                                         <  2:3981665/150‑1 (MQ=255)

                                              cGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCg                                                                                                         <  2:299764/150‑1 (MQ=255)

                                              cGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCg                                                                                                         >  1:766419/1‑150 (MQ=255)

                                              cGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCg                                                                                                         <  1:3766903/150‑1 (MQ=255)

                                              cGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCg                                                                                                         <  2:907254/150‑1 (MQ=255)

                                                  atatCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCa                                                                                                     >  1:499063/1‑150 (MQ=255)

                                                       cGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTgg                                                                                                >  1:1950876/1‑150 (MQ=255)

                                                       cGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTgg                                                                                                >  2:1857089/1‑150 (MQ=255)

                                                           cGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAAt                                                                                            <  1:3511117/150‑1 (MQ=255)

                                                                 cGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGa                                                                                      <  1:3215321/150‑1 (MQ=255)

                                                                  gTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGaa                                                                                     >  1:3693972/1‑150 (MQ=255)

                                                                   tCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAAc                                                                                    <  1:1328695/150‑1 (MQ=255)

                                                                     gATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGt                                                                                  >  2:3465113/1‑150 (MQ=255)

                                                                      aTTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTa                                                                                 >  2:3851302/1‑150 (MQ=255)

                                                                      aTTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTa                                                                                 >  1:1569655/1‑150 (MQ=255)

                                                                          ccAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCg                                                                             >  1:2802474/1‑150 (MQ=255)

                                                                            aGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCggg                                                                           >  1:792461/1‑150 (MQ=255)

                                                                              gTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTa                                                                         <  1:1389624/150‑1 (MQ=255)

                                                                              gTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTa                                                                         <  2:4209623/150‑1 (MQ=255)

                                                                                 accaccTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTacac                                                                      >  1:2715467/1‑150 (MQ=255)

                                                                                    accTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGaa                                                                   >  1:1677232/1‑150 (MQ=255)

                                                                                    accTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGaa                                                                   >  1:3936474/1‑150 (MQ=255)

                                                                                      ctactaCAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAgg                                                                 >  1:986128/1‑150 (MQ=255)

                                                                                       tactacAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAggg                                                                <  1:557828/150‑1 (MQ=255)

                                                                                         ctacAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCa                                                              <  1:94130/150‑1 (MQ=255)

                                                                                              gCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGc                                                         <  2:4252379/150‑1 (MQ=255)

                                                                                               cAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCt                                                        >  1:83206/1‑150 (MQ=255)

                                                                                                aaCATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTg                                                       >  1:1514907/1‑150 (MQ=255)

                                                                                                  cATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGaa                                                     <  1:2562025/150‑1 (MQ=255)

                                                                                                   aTTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGaaa                                                    >  1:267738/1‑150 (MQ=255)

                                                                                                   aTTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGaaa                                                    >  1:1042361/1‑150 (MQ=255)

                                                                                                      tGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAAtt                                                 <  2:3438742/150‑1 (MQ=255)

                                                                                                        cccAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATtct                                               <  1:4363293/150‑1 (MQ=255)

                                                                                                         ccAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATtctc                                              <  1:2347149/150‑1 (MQ=255)

                                                                                                          cAAATGGTGCTTATCAGGGTTATGGCGCACCGAACGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCg                                             <  1:1403125/150‑1 (MQ=255)

                                                                                                          cAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCg                                             >  1:4396902/1‑150 (MQ=255)

                                                                                                          cAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCg                                             >  1:649070/1‑150 (MQ=255)

                                                                                                          cAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCg                                             >  2:2726802/1‑150 (MQ=255)

                                                                                                          cAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCg                                             <  1:3183120/150‑1 (MQ=255)

                                                                                                           aaaTGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATTGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCgg                                            >  2:2777855/1‑150 (MQ=255)

                                                                                                           aaaTGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCgg                                            >  1:1599394/1‑150 (MQ=255)

                                                                                                                gtgtTTATCAGGGTTTTGGCGCACCGATTGGTATCTTCGCTATCACCTTGGCTTTTGCGGTTCTGGCTGATCTGTTAGAGATCGACCTTCTGGATATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCaa                                       <  2:3632700/150‑1 (MQ=255)

                                                                                                                 tGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAAt                                      <  2:543006/150‑1 (MQ=255)

                                                                                                                    ttATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCgg                                   >  1:3357634/1‑150 (MQ=255)

                                                                                                                    ttATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCgg                                   <  2:3027166/150‑1 (MQ=255)

                                                                                                                     tATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAACATTCTCGGTGCAATCGGt                                  >  2:2421079/1‑150 (MQ=255)

                                                                                                                     tATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGt                                  <  2:4036302/150‑1 (MQ=255)

                                                                                                                     tATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGt                                  >  1:1540335/1‑150 (MQ=255)

                                                                                                                        cAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGaa                               >  1:3993018/1‑150 (MQ=255)

                                                                                                                         aGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAg                              <  2:499063/150‑1 (MQ=255)

                                                                                                                          gggTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAgg                             <  2:1325606/150‑1 (MQ=255)

                                                                                                                           ggTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGt                            <  2:359500/150‑1 (MQ=255)

                                                                                                                           ggTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGt                            <  1:1805123/150‑1 (MQ=255)

                                                                                                                           ggTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGt                            <  1:815780/150‑1 (MQ=255)

                                                                                                                             ttATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGtca                          >  2:4111650/1‑148 (MQ=255)

                                                                                                                              tATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTaaa                         >  2:1304450/1‑150 (MQ=255)

                                                                                                                               aTGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAg                        >  1:4124821/1‑150 (MQ=255)

                                                                                                                                     cACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGa                  <  2:64188/150‑1 (MQ=255)

                                                                                                                                        cGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCt               >  2:2272116/1‑150 (MQ=255)

                                                                                                                                         gAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTc              >  1:2885213/1‑150 (MQ=255)

                                                                                                                                         gAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTc              >  2:755144/1‑150 (MQ=255)

                                                                                                                                           aaGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCg            <  2:4393780/150‑1 (MQ=255)

                                                                                                                                               tAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttcc        <  2:766293/150‑1 (MQ=255)

                                                                                                                                                aaCTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttcct       <  2:307379/150‑1 (MQ=255)

                                                                                                                                                aaCTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttcct       <  2:6530/150‑1 (MQ=255)

                                                                                                                                                aaCTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttcct       <  2:2604857/150‑1 (MQ=255)

                                                                                                                                                 aCTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttcctt      >  1:4311532/1‑150 (MQ=255)

                                                                                                                                                   ttCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttccttcc    <  2:2010761/150‑1 (MQ=255)

                                                                                                                                                   ttCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAAGTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttccttcc    <  2:2010735/150‑1 (MQ=255)

AACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGTTCCTTCCGC  >  CP009273/3016223‑3016521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A