Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,016,372 C→A 19.5% F566L (TTC→TTA)  xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,016,3720CA19.5% 889.6 / 160.8 320F566L (TTC→TTA) xdhDputative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
Reads supporting (aligned to +/- strand):  ref base C (126/131);  new base A (28/35);  total (154/166)
Fisher's exact test for biased strand distribution p-value = 5.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

ACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGTTCCTTCCGC  >  CP009273/3016224‑3016521
                                                                                                                                                    |                                                                                                                                                     
aCCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCg                                                                                                                                                      >  1:2535434/1‑150 (MQ=255)
 ccACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGc                                                                                                                                                     >  1:984208/1‑150 (MQ=255)
  cactcactCACCGTACCGTGTCACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCt                                                                                                                                                    <  2:2713685/150‑1 (MQ=255)
       actcacCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCAc                                                                                                                                               >  2:2184657/1‑150 (MQ=255)
        ctcacCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCAcc                                                                                                                                              <  1:4322968/150‑1 (MQ=255)
           accgtaccgtGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATg                                                                                                                                           >  2:2338037/1‑150 (MQ=255)
             cgtaccgtGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGc                                                                                                                                         <  1:227363/150‑1 (MQ=255)
              gtaccgtGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCa                                                                                                                                        <  1:726950/150‑1 (MQ=255)
                   gtgtAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGc                                                                                                                                   <  2:1854611/150‑1 (MQ=255)
                    tgtAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCg                                                                                                                                  <  1:1001097/150‑1 (MQ=255)
                         cGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACt                                                                                                                             <  1:899203/150‑1 (MQ=255)
                          ggACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCGATCACCATGGCATTAGCGGAACTg                                                                                                                            >  2:435543/1‑150 (MQ=255)
                             ccGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCt                                                                                                                         <  2:3681838/150‑1 (MQ=255)
                             ccGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCt                                                                                                                         <  1:1423138/150‑1 (MQ=255)
                               ggCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTTTCACCATGGCATTAGCGGAACTGGCTGa                                                                                                                       <  1:262880/150‑1 (MQ=255)
                               ggCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGa                                                                                                                       <  2:2224630/150‑1 (MQ=255)
                                      tCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTa                                                                                                                <  1:2209929/150‑1 (MQ=255)
                                      tCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTa                                                                                                                >  2:1839581/1‑150 (MQ=255)
                                      tCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAAGGGTGCTTATCCGGGTTATGGCGCACGGAAAGGTAACTTCGCTATCACCATGGCCTTAGCGGAACTGGCTGAACAGTTa                                                                                                                <  1:3501708/150‑1 (MQ=255)
                                          tGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGa                                                                                                            >  1:769384/1‑150 (MQ=255)
                                           gCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGAt                                                                                                           <  2:1029350/150‑1 (MQ=255)
                                                tataTCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGAcc                                                                                                      >  2:1071343/1‑150 (MQ=255)
                                                  tatCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGGACTGGCTGAACAGTTACAGATCGACCaa                                                                                                    >  2:3482370/1‑150 (MQ=255)
                                                  tatCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCaa                                                                                                    <  2:1219062/150‑1 (MQ=255)
                                                     ccGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTg                                                                                                 <  2:71247/150‑1 (MQ=255)
                                                        tGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGaa                                                                                              <  2:2827630/150‑1 (MQ=255)
                                                        tGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTACCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGaa                                                                                              >  2:4329535/1‑150 (MQ=255)
                                                          cGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAAt                                                                                            <  1:2636327/150‑1 (MQ=255)
                                                          cGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAAt                                                                                            <  1:2431464/150‑1 (MQ=255)
                                                          cGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAAt                                                                                            <  1:1525290/150‑1 (MQ=255)
                                                           gATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAAtt                                                                                           <  2:3080984/150‑1 (MQ=255)
                                                           gATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAAtt                                                                                           <  2:818967/150‑1 (MQ=255)
                                                             tAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGTACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTAt                                                                                         <  2:2513884/150‑1 (MQ=255)
                                                             tAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTAt                                                                                         >  1:1707526/1‑150 (MQ=255)
                                                               aCGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCg                                                                                       >  1:1182399/1‑150 (MQ=255)
                                                                cGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGa                                                                                      >  1:2904054/1‑150 (MQ=255)
                                                                 gTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGaa                                                                                     >  2:461446/1‑150 (MQ=255)
                                                                 gTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGaa                                                                                     <  1:39863/150‑1 (MQ=255)
                                                                 gTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGaa                                                                                     <  1:4176769/150‑1 (MQ=255)
                                                                 gTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGaa                                                                                     >  1:2326168/1‑150 (MQ=255)
                                                                   cGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACg                                                                                   <  2:560325/150‑1 (MQ=255)
                                                                     aTTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTa                                                                                 <  2:652678/150‑1 (MQ=255)
                                                                      tttCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTaa                                                                                <  2:1565280/150‑1 (MQ=255)
                                                                       ttCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAAc                                                                               <  1:830/150‑1 (MQ=255)
                                                                       ttCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAAc                                                                               <  1:1083/150‑1 (MQ=255)
                                                                             gTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTa                                                                         >  1:2061653/1‑150 (MQ=255)
                                                                                    ccTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAg                                                                  <  1:1410199/150‑1 (MQ=255)
                                                                                      tactacAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAggg                                                                <  1:1650037/150‑1 (MQ=255)
                                                                                      tactacAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAggg                                                                <  1:1937328/150‑1 (MQ=255)
                                                                                       actacAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGc                                                               <  2:1468725/150‑1 (MQ=255)
                                                                                         tacAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCaa                                                             <  2:3526372/150‑1 (MQ=255)
                                                                                          acAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAag                                                            >  1:1094553/1‑150 (MQ=255)
                                                                                           cAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAaga                                                           <  2:1121285/150‑1 (MQ=255)
                                                                                              cAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCt                                                        >  2:2570874/1‑150 (MQ=255)
                                                                                               aaCATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTg                                                       >  2:2940961/1‑150 (MQ=255)
                                                                                                      gCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATtc                                                <  1:4478417/150‑1 (MQ=255)
                                                                                                       cccAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATtct                                               <  1:1260463/150‑1 (MQ=255)
                                                                                                        ccAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATtctc                                              >  2:2038486/1‑150 (MQ=255)
                                                                                                          aaaTGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCgg                                            >  1:711034/1‑150 (MQ=255)
                                                                                                           aaTGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGt                                           <  2:2415881/150‑1 (MQ=255)
                                                                                                              ggTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCa                                        <  1:1112597/150‑1 (MQ=255)
                                                                                                              ggTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCa                                        <  1:3397580/150‑1 (MQ=255)
                                                                                                              ggTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCa                                        >  2:1552681/1‑150 (MQ=255)
                                                                                                              ggTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCa                                        >  1:3145474/1‑150 (MQ=255)
                                                                                                               gTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCaa                                       >  1:3612500/1‑150 (MQ=255)
                                                                                                               gTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCaa                                       >  2:2668784/1‑150 (MQ=255)
                                                                                                                tGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAAt                                      >  1:3726984/1‑150 (MQ=255)
                                                                                                                tGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAAt                                      <  1:4385684/150‑1 (MQ=255)
                                                                                                                 gCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATc                                     >  2:1354535/1‑150 (MQ=255)
                                                                                                                 gCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATc                                     >  1:491989/1‑150 (MQ=255)
                                                                                                                   ttATCAGGGTTATGGCGCACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCgg                                   <  1:4041215/150‑1 (MQ=255)
                                                                                                                        aGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAg                              >  2:3836434/1‑150 (MQ=255)
                                                                                                                        aGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAg                              >  1:636146/1‑150 (MQ=255)
                                                                                                                             tATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTaaa                         >  2:873982/1‑150 (MQ=255)
                                                                                                                             tATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTaaa                         <  1:2998360/150‑1 (MQ=255)
                                                                                                                              aTGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAg                        <  1:856477/150‑1 (MQ=255)
                                                                                                                              aTGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAg                        <  1:2776370/150‑1 (MQ=255)
                                                                                                                                ggCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAgcg                      <  2:3160389/150‑1 (MQ=255)
                                                                                                                                  cgcACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGcc                    <  2:4458204/150‑1 (MQ=255)
                                                                                                                                  cgcACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGcc                    >  2:1868354/1‑150 (MQ=255)
                                                                                                                                  cgcACCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGcc                    <  2:4051011/150‑1 (MQ=255)
                                                                                                                                   gcACCGAACGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCg                   <  1:3278907/150‑1 (MQ=255)
                                                                                                                                     aCCGAAAGGTAACTTAGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGAc                 >  2:417894/1‑150 (MQ=255)
                                                                                                                                       cGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCt               >  1:1658989/1‑150 (MQ=255)
                                                                                                                                        gAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTc              >  2:3931481/1‑150 (MQ=255)
                                                                                                                                         aaaTGTAACTTCGATATAACCTTGTCATTAGCGTAACTGGCTGAACATTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACTAAGGGCAAGAGCTGAAAATTCTCGGTGAAATCGGTGAAGGTAAAGCGCCGACCTcc             <  1:219618/150‑1 (MQ=255)
                                                                                                                                         aaaGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTcc             <  1:2117151/150‑1 (MQ=255)
                                                                                                                                         aaaGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGAAATCGGTGAAGGTAAAGCGCCGACCTcc             <  1:2891960/150‑1 (MQ=255)
                                                                                                                                          aaGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCACTCGGTGAAGGTAAAGCGCCGACCTCCg            >  1:383023/1‑150 (MQ=255)
                                                                                                                                          aaGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCg            >  2:3733479/1‑150 (MQ=255)
                                                                                                                                          aaGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCg            >  1:1600349/1‑150 (MQ=255)
                                                                                                                                          aaGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCg            >  1:3762419/1‑150 (MQ=255)
                                                                                                                                            gttAACTTCGATATCACCATGGCATTAGCGGAACTGGCTTAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGtt          <  1:1924269/148‑1 (MQ=255)
                                                                                                                                             gTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttc         >  2:3409779/1‑150 (MQ=255)
                                                                                                                                             gTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttc         >  2:3176143/1‑150 (MQ=255)
                                                                                                                                              tAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttcc        >  1:3190676/1‑150 (MQ=255)
                                                                                                                                               aaCTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttcct       >  2:1706949/1‑150 (MQ=255)
                                                                                                                                                aCTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGTAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttcctt      <  1:958970/150‑1 (MQ=255)
                                                                                                                                                  ttCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttccttcc    <  2:3804208/150‑1 (MQ=255)
                                                                                                                                                  ttCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGttccttcc    >  1:2699596/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACCACTCACTCACCGTACCGTGTAACGGACCGGCGCTGTCGCTGCCGTTATATCCGTGCGATAACGTCGATTTCCAGGTCACCACCTACTACAGCAACATTTGCCCAAATGGTGCTTATCAGGGTTATGGCGCACCGAAAGGTAACTTCGCTATCACCATGGCATTAGCGGAACTGGCTGAACAGTTACAGATCGACCAACTGGAAATTATCGAACGTAACCGGGTACACGAAGGGCAAGAGCTGAAAATTCTCGGTGCAATCGGTGAAGGTAAAGCGCCGACCTCCGTTCCTTCCGC  >  CP009273/3016224‑3016521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A