Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,278,325 T→A 6.5% C212S (TGC→AGC)  nrfA → nitrite reductase, formate‑dependent, cytochrome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,278,3250TA 6.5% 922.3 / 38.5 278C212S (TGC→AGC) nrfAnitrite reductase, formate‑dependent, cytochrome
Reads supporting (aligned to +/- strand):  ref base T (140/120);  new base A (9/9);  total (149/129)
Fisher's exact test for biased strand distribution p-value = 8.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 281 total aligned reads displayed.

GCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAA  >  CP009273/4278179‑4278474
                                                                                                                                                  |                                                                                                                                                     
gCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGcc                                                                                                                                                    >  2:2104371/1‑150 (MQ=255)
    tAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCAtgt                                                                                                                                                >  2:4322465/1‑150 (MQ=255)
    tAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTAGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCAtgt                                                                                                                                                >  2:1789295/1‑150 (MQ=255)
     aaCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCAtgtg                                                                                                                                               >  2:2200813/1‑150 (MQ=255)
     aaCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCAtgtg                                                                                                                                               <  2:1962422/150‑1 (MQ=255)
     aaCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCAtgtg                                                                                                                                               <  2:1962186/150‑1 (MQ=255)
         ccGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGt                                                                                                                                           >  2:3732139/1‑150 (MQ=255)
         ccGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGt                                                                                                                                           >  2:3951889/1‑150 (MQ=255)
           gCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTAt                                                                                                                                         >  2:1312162/1‑150 (MQ=255)
            ccTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTAtt                                                                                                                                        >  2:1195547/1‑150 (MQ=255)
             ctctCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTa                                                                                                                                       <  1:1777993/150‑1 (MQ=255)
               ctcCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACt                                                                                                                                     <  2:3795516/150‑1 (MQ=255)
               ctcCAGAGGTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACt                                                                                                                                     <  2:4245753/150‑1 (MQ=255)
                tcCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACtt                                                                                                                                    <  1:2202927/150‑1 (MQ=255)
                 ccAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTc                                                                                                                                   >  1:752602/1‑150 (MQ=255)
                   agagTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGa                                                                                                                                 <  1:1855449/150‑1 (MQ=255)
                   agagTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGa                                                                                                                                 <  1:4377698/150‑1 (MQ=255)
                   agagTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGa                                                                                                                                 <  1:3657151/150‑1 (MQ=255)
                   agagTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGa                                                                                                                                 >  2:3133170/1‑150 (MQ=255)
                   agagTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCAGGTGGAGTATTACTTCGa                                                                                                                                 >  2:2494246/1‑150 (MQ=255)
                   agagTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGACATGTGGAGTATTACTTCGa                                                                                                                                 >  1:4239519/1‑150 (MQ=255)
                    gagTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGAc                                                                                                                                >  1:3501188/1‑150 (MQ=255)
                       ttCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGc                                                                                                                             >  2:3745959/1‑150 (MQ=255)
                           ccAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCaaaa                                                                                                                         >  1:769428/1‑150 (MQ=255)
                            cAAAGGCAAACCGGATTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAACGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGcccaaa                                                                                                                        >  2:4536584/1‑145 (MQ=255)
                              aaGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACa                                                                                                                      <  1:486569/150‑1 (MQ=255)
                               aGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACaa                                                                                                                     >  1:1441592/1‑150 (MQ=255)
                               aGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACaa                                                                                                                     >  1:1441237/1‑150 (MQ=255)
                                ggCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACaaa                                                                                                                    <  1:2923256/150‑1 (MQ=255)
                                ggCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACaaa                                                                                                                    >  2:4418954/1‑150 (MQ=255)
                                  cAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGc                                                                                                                  >  1:4512409/1‑150 (MQ=255)
                                  cAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGc                                                                                                                  <  2:1980315/150‑1 (MQ=255)
                                    aaCCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCgg                                                                                                                >  1:3947611/1‑150 (MQ=255)
                                        ggAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTaa                                                                                                            >  1:2542084/1‑150 (MQ=255)
                                        ggAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTaa                                                                                                            <  1:866072/150‑1 (MQ=255)
                                         gAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTaaa                                                                                                           <  2:344785/150‑1 (MQ=255)
                                         gAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTaaa                                                                                                           <  2:1945561/150‑1 (MQ=255)
                                         gAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTaaa                                                                                                           <  2:2856045/150‑1 (MQ=255)
                                         gAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTaaa                                                                                                           <  2:2585248/150‑1 (MQ=255)
                                           gTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAAtt                                                                                                         >  2:3359947/1‑150 (MQ=255)
                                           gTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAAtt                                                                                                         >  1:2152182/1‑150 (MQ=255)
                                            ttAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTc                                                                                                        <  2:2446025/150‑1 (MQ=255)
                                             tAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTcc                                                                                                       <  1:4322465/150‑1 (MQ=255)
                                                cccTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGt                                                                                                    <  2:4048651/150‑1 (MQ=255)
                                                  cTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTgg                                                                                                  >  2:4017784/1‑150 (MQ=255)
                                                    ttCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGa                                                                                                <  1:3359010/150‑1 (MQ=255)
                                                          tccgtATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACgg                                                                                          >  1:2216873/1‑150 (MQ=255)
                                                            cgtATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCa                                                                                        >  1:2479525/1‑150 (MQ=255)
                                                              tATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGCAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATg                                                                                      >  1:2963140/1‑150 (MQ=255)
                                                               aTGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGa                                                                                     <  2:2970726/150‑1 (MQ=255)
                                                                tGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGaa                                                                                    >  2:231624/1‑150 (MQ=255)
                                                                  cGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAg                                                                                  >  1:1032174/1‑150 (MQ=255)
                                                                    gCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTc                                                                                <  1:1931036/150‑1 (MQ=255)
                                                                       cgcgcgATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGaa                                                                             >  2:4235725/1‑150 (MQ=255)
                                                                       cgcgcgATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGaa                                                                             <  2:2676312/150‑1 (MQ=255)
                                                                             aTGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATg                                                                       >  1:3249795/1‑150 (MQ=255)
                                                                              tGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATgg                                                                      <  2:2718842/150‑1 (MQ=255)
                                                                                 aaGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCCGTGCCATGTGGAGTATTACTACGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGc                                                                   >  2:3888103/1‑150 (MQ=255)
                                                                                  aGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCa                                                                  >  1:3428725/1‑150 (MQ=255)
                                                                                     cATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCCATCGATGGTTTGCGGCCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTa                                                               >  2:204003/1‑150 (MQ=255)
                                                                                     cATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTa                                                               >  1:2189398/1‑150 (MQ=255)
                                                                                      aTTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTAt                                                              <  1:2709010/150‑1 (MQ=255)
                                                                                      aTTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTAt                                                              >  2:221631/1‑150 (MQ=255)
                                                                                      aTTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTAt                                                              <  1:2084160/150‑1 (MQ=255)
                                                                                        tGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTa                                                            >  1:4492227/1‑150 (MQ=255)
                                                                                        tGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTa                                                            >  2:354828/1‑150 (MQ=255)
                                                                                         ggTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTAc                                                           <  1:762093/150‑1 (MQ=255)
                                                                                          gTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACg                                                          <  1:1362923/150‑1 (MQ=255)
                                                                                            aaaCCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGAc                                                        <  1:1877490/150‑1 (MQ=255)
                                                                                            aaaCCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGAc                                                        <  2:1010507/150‑1 (MQ=255)
                                                                                                  tttGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAAtt                                                  <  1:2317758/150‑1 (MQ=255)
                                                                                                  tttGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAAtt                                                  <  2:1637795/150‑1 (MQ=255)
                                                                                                   ttGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTg                                                 >  2:3201059/1‑150 (MQ=255)
                                                                                                     gagaAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGcc                                               >  1:3642969/1‑150 (MQ=255)
                                                                                                       gaAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAACAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCtt                                             <  2:3033840/150‑1 (MQ=255)
                                                                                                       gaAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCtt                                             >  1:1285111/1‑150 (MQ=255)
                                                                                                       gaAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCtt                                             <  1:1336749/150‑1 (MQ=255)
                                                                                                        aaaGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTtc                                            >  2:1395257/1‑150 (MQ=255)
                                                                                                        aaaGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTtc                                            >  2:3940395/1‑150 (MQ=255)
                                                                                                        aaaGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTtc                                            <  2:3806121/150‑1 (MQ=255)
                                                                                                         aaGCCTGAAGTTTCGACCAGCAATCGATGGTTTGCGTTCAGTGCCATGTTGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTtct                                           <  1:1195547/150‑1 (MQ=255)
                                                                                                              ggACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGAc                                      <  1:3460229/150‑1 (MQ=255)
                                                                                                                   tttCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGAc                                 >  1:3296527/1‑150 (MQ=255)
                                                                                                                     tCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTa                               <  1:1703095/150‑1 (MQ=255)
                                                                                                                       gACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAAc                             >  1:3062868/1‑150 (MQ=255)
                                                                                                                                tCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCg                    <  1:2777993/150‑1 (MQ=255)
                                                                                                                                 cGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGGGGTTAAATTCCCGTGGGATGACGGCATCAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGa                   >  1:4444740/1‑150 (MQ=255)
                                                                                                                                 cGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGa                   >  2:2240391/1‑150 (MQ=255)
                                                                                                                                 cGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGa                   >  2:3312480/1‑150 (MQ=255)
                                                                                                                                   aTGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGaaa                 >  1:3140066/1‑150 (MQ=255)
                                                                                                                                    tGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGaaaa                >  1:2328605/1‑150 (MQ=255)
                                                                                                                                     ggTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTAGGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAAc               <  1:1006602/150‑1 (MQ=255)
                                                                                                                                       tttGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGc             >  1:2908562/1‑150 (MQ=255)
                                                                                                                                        ttGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGcc            <  2:3249795/150‑1 (MQ=255)
                                                                                                                                            ggTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATg        <  2:549480/150‑1 (MQ=255)
                                                                                                                                               cAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTg     <  2:817211/150‑1 (MQ=255)
                                                                                                                                               cAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAACATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTg     >  2:429485/1‑150 (MQ=255)
                                                                                                                                                aGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGa    <  1:2422462/150‑1 (MQ=255)
                                                                                                                                                  tGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGaaa  >  2:4150074/1‑150 (MQ=255)
                                                                                                                                                  tGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGaaa  <  1:3875769/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAAA  >  CP009273/4278179‑4278474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A