Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,278,325 T→A 38.8% C212S (TGC→AGC)  nrfA → nitrite reductase, formate‑dependent, cytochrome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,278,3250TA38.8% 248.7 / inf 284C212S (TGC→AGC) nrfAnitrite reductase, formate‑dependent, cytochrome
Reads supporting (aligned to +/- strand):  ref base T (73/101);  new base A (55/55);  total (128/156)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 286 total aligned reads displayed.

ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAA  >  CP009273/4278176‑4278473
                                                                                                                                                     |                                                                                                                                                    
aTTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGt                                                                                                                                                      <  1:3941903/150‑1 (MQ=255)
 ttGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTg                                                                                                                                                     <  2:1905079/150‑1 (MQ=255)
  tGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGc                                                                                                                                                    >  2:2106215/1‑150 (MQ=255)
   gCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGcc                                                                                                                                                   <  1:303554/150‑1 (MQ=255)
    ccATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCa                                                                                                                                                  <  1:2800504/150‑1 (MQ=255)
      aTAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCAtg                                                                                                                                                <  2:1463887/150‑1 (MQ=255)
       tAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCAtgt                                                                                                                                               >  2:3408453/1‑150 (MQ=255)
        aaCACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCAtgtg                                                                                                                                              <  1:500255/150‑1 (MQ=255)
            ccGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGt                                                                                                                                          <  1:1636044/150‑1 (MQ=255)
               ccTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTAtt                                                                                                                                       <  2:3665322/150‑1 (MQ=255)
               ccTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTAtt                                                                                                                                       <  2:2306475/150‑1 (MQ=255)
                ctctCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGTCGTTTCGACCAGCAATCGATTGTTTGCGGTCAGTGCCATGTGGAGTATTa                                                                                                                                      <  1:3577596/150‑1 (MQ=255)
                ctctCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTa                                                                                                                                      <  1:2487914/150‑1 (MQ=255)
                 tctcCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTAc                                                                                                                                     <  1:142779/150‑1 (MQ=255)
                  ctcCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACt                                                                                                                                    <  2:317865/150‑1 (MQ=255)
                        agTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACg                                                                                                                              <  2:1362740/150‑1 (MQ=255)
                        agTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACg                                                                                                                              <  1:1090707/150‑1 (MQ=255)
                        agTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACg                                                                                                                              <  1:3238480/150‑1 (MQ=255)
                          ttCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGc                                                                                                                            <  2:4090554/150‑1 (MQ=255)
                           tCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCa                                                                                                                           <  1:3864680/150‑1 (MQ=255)
                             gCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCaaa                                                                                                                         >  1:213583/1‑150 (MQ=255)
                                 aaGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACa                                                                                                                     >  1:2775892/1‑150 (MQ=255)
                                  aGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGACAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACaa                                                                                                                    >  2:1779938/1‑150 (MQ=255)
                                        aCCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGt                                                                                                              >  2:4152146/1‑150 (MQ=255)
                                        aCCGGAGTTAACCCGTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGt                                                                                                              <  2:1712405/150‑1 (MQ=255)
                                         ccGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCACAAACAAAGCGGtt                                                                                                             >  2:2578515/1‑150 (MQ=255)
                                          cGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTa                                                                                                            <  1:797733/150‑1 (MQ=255)
                                          cGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTa                                                                                                            <  2:4032498/150‑1 (MQ=255)
                                          cGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTa                                                                                                            <  2:476548/150‑1 (MQ=255)
                                           ggAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTaa                                                                                                           >  1:535737/1‑150 (MQ=255)
                                            gAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTaaa                                                                                                          >  1:1061210/1‑150 (MQ=255)
                                            gAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTaaa                                                                                                          >  1:3543781/1‑150 (MQ=255)
                                             aGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAAt                                                                                                         <  2:3575200/150‑1 (MQ=255)
                                                tAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTcc                                                                                                      >  1:2293989/1‑150 (MQ=255)
                                                 aaCCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTccc                                                                                                     <  1:3146504/150‑1 (MQ=255)
                                                 aaCCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTccc                                                                                                     >  2:2979276/1‑150 (MQ=255)
                                                  aCCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCg                                                                                                    <  1:864113/150‑1 (MQ=255)
                                                    ccTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTg                                                                                                  <  2:3915207/150‑1 (MQ=255)
                                                     cTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTgg                                                                                                 <  2:690158/150‑1 (MQ=255)
                                                       ttCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGa                                                                                               <  1:1108531/150‑1 (MQ=255)
                                                       ttCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGa                                                                                               <  2:3779417/150‑1 (MQ=255)
                                                        tCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGAt                                                                                              <  2:1945796/150‑1 (MQ=255)
                                                        tCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGAt                                                                                              >  1:3780864/1‑150 (MQ=255)
                                                         cccGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATg                                                                                             >  1:396577/1‑150 (MQ=255)
                                                                gtATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCAt                                                                                      >  2:1495876/1‑150 (MQ=255)
                                                                 tATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCTATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGTCTAAAACATATCGGTTAAATTCCCGTGGGATGACGGCATg                                                                                     <  2:2913548/150‑1 (MQ=255)
                                                                 tATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATg                                                                                     <  1:564718/150‑1 (MQ=255)
                                                                  aTGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGa                                                                                    >  2:2031561/1‑150 (MQ=255)
                                                                  aTGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGC‑AAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGaa                                                                                   >  1:1504547/1‑150 (MQ=255)
                                                                     cGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAg                                                                                 <  2:1241111/150‑1 (MQ=255)
                                                                      ggCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGt                                                                                >  1:2084089/1‑150 (MQ=255)
                                                                      ggCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGt                                                                                >  1:1793175/1‑150 (MQ=255)
                                                                            cgcgATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGaaaa                                                                          >  2:2424840/1‑150 (MQ=255)
                                                                               gATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAatat                                                                       <  2:3012627/150‑1 (MQ=255)
                                                                                aTGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATg                                                                      <  1:1754134/150‑1 (MQ=255)
                                                                                aTGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATg                                                                      >  2:1107197/1‑150 (MQ=255)
                                                                                  ggAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGa                                                                    >  2:625998/1‑150 (MQ=255)
                                                                                    aaGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGc                                                                  <  1:2525186/150‑1 (MQ=255)
                                                                                    aaGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGc                                                                  <  1:97151/150‑1 (MQ=255)
                                                                                     aGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCa                                                                 <  2:1684723/150‑1 (MQ=255)
                                                                                      gCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGAGGGAAGACGGCAGGAACGTCGAAAATATGGAGCAg                                                                >  1:3079912/1‑150 (MQ=255)
                                                                                        cATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTa                                                              >  1:3205465/1‑150 (MQ=255)
                                                                                         aTTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTAt                                                             <  1:22826/150‑1 (MQ=255)
                                                                                          ttGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGAGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTAtt                                                            <  1:365380/150‑1 (MQ=255)
                                                                                          ttGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTAtt                                                            <  1:2043403/150‑1 (MQ=255)
                                                                                           tGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTa                                                           <  2:2151713/150‑1 (MQ=255)
                                                                                           tGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTa                                                           <  2:3234308/150‑1 (MQ=255)
                                                                                           tGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTa                                                           >  2:1235550/1‑150 (MQ=255)
                                                                                             gTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACg                                                         <  1:151999/150‑1 (MQ=255)
                                                                                                aaCCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACa                                                      >  1:3518148/1‑150 (MQ=255)
                                                                                                 aCCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACaa                                                     >  1:2408914/1‑150 (MQ=255)
                                                                                                   cTTTTGAGAAAGCCGGACTTTTCGACCAGCAATCGATGGTTTGCTGTCAGTGACATGTGGAGTATTACTTCTACGTCAAAAACAAAGCTGTTAAATTCCCGTGGGATGACTTCATGAATGTCGAAAATATGGAGCAGTATTACGACaaaa                                                   <  1:2899933/150‑1 (MQ=255)
                                                                                                   cTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACaaaa                                                   >  1:1273750/1‑150 (MQ=255)
                                                                                                   cTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGACGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACaaaa                                                   >  2:3662334/1‑150 (MQ=255)
                                                                                                      ttGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTg                                                <  2:3615415/150‑1 (MQ=255)
                                                                                                       tGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGc                                               <  2:3342106/150‑1 (MQ=255)
                                                                                                       tGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGc                                               <  2:3342122/150‑1 (MQ=255)
                                                                                                        gagaAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGcc                                              <  1:938970/150‑1 (MQ=255)
                                                                                                         agaAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCt                                             >  1:33533/1‑150 (MQ=255)
                                                                                                          gaAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGACAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCtt                                            >  2:3924916/1‑150 (MQ=255)
                                                                                                           aaaGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTtc                                           <  1:495195/150‑1 (MQ=255)
                                                                                                            aaGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTtct                                          <  2:1920763/150‑1 (MQ=255)
                                                                                                             aGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTtctc                                         >  1:1667002/1‑150 (MQ=255)
                                                                                                                 ggACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGAc                                     >  1:1186503/1‑150 (MQ=255)
                                                                                                                 ggACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGAc                                     >  1:1298190/1‑150 (MQ=255)
                                                                                                                   aCGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTg                                   >  1:2728680/1‑150 (MQ=255)
                                                                                                                    cGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTgg                                  <  1:1299433/150‑1 (MQ=255)
                                                                                                                     gTTTCGACCAGCAATCGATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGa                                 <  2:541433/150‑1 (MQ=255)
                                                                                                                           aCCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACt                           <  2:3495779/150‑1 (MQ=255)
                                                                                                                            ccAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTc                          <  2:577998/150‑1 (MQ=255)
                                                                                                                              aGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTccc                        >  1:3793881/1‑150 (MQ=255)
                                                                                                                                  aTCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTc                    >  2:265109/1‑150 (MQ=255)
                                                                                                                                     gATGGTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGaa                 >  2:2226877/1‑150 (MQ=255)
                                                                                                                                        ggTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAAc              <  2:938965/150‑1 (MQ=255)
                                                                                                                                        ggTTTGCGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAATCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAAc              <  1:2018311/150‑1 (MQ=255)
                                                                                                                                             gCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCaaa         <  1:3180442/150‑1 (MQ=255)
                                                                                                                                              cGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAAt        >  2:1580638/1‑150 (MQ=255)
                                                                                                                                              cGGTCAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAAt        >  2:1807356/1‑150 (MQ=255)
                                                                                                                                                 tCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCt     >  1:3215008/1‑150 (MQ=255)
                                                                                                                                                  cAGAGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTg    >  1:6887/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTTTCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGACGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGCAAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAGTATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTGAA  >  CP009273/4278176‑4278473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A