Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F121 I0 R1	856	273.6	9081716	94.1%	8545894	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	530,466	C→A	89.5%	F365L (TTC→TTA)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	530,466	0	C	A	89.5%	758.6 / 76.9	258	F365L (TTC→TTA)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand): ref base C (12/15); new base A (120/111); total (132/126)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

GGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCA  >  CP009273/530320‑530611

   aTTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAAc                                                                                                                                             <  2:1173726/150‑1 (MQ=255)

   aTTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAAc                                                                                                                                             >  2:1304996/1‑150 (MQ=255)

   aTTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAAc                                                                                                                                             <  1:2852307/150‑1 (MQ=255)

   aTTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAAc                                                                                                                                             >  2:3691287/1‑150 (MQ=255)

     tGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGt                                                                                                                                           <  1:1665411/150‑1 (MQ=255)

       tctcTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGc                                                                                                                                         <  1:253962/150‑1 (MQ=255)

        ctctGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGcc                                                                                                                                        <  1:106506/150‑1 (MQ=255)

        ctctGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGcc                                                                                                                                        >  1:2423219/1‑150 (MQ=255)

            gaggCTACAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTg                                                                                                                                    <  1:2678029/147‑1 (MQ=255)

            gATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTg                                                                                                                                    <  2:3038551/150‑1 (MQ=255)

            gATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTg                                                                                                                                    <  2:2370710/150‑1 (MQ=255)

            gATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTg                                                                                                                                    >  1:3261197/1‑150 (MQ=255)

             aTGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGa                                                                                                                                   >  2:254578/1‑150 (MQ=255)

               gCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGaaa                                                                                                                                 >  1:4195960/1‑150 (MQ=255)

                  aaaGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCg                                                                                                                              >  2:801296/1‑150 (MQ=255)

                  aaaGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCg                                                                                                                              >  1:3147316/1‑150 (MQ=255)

                    aGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCa                                                                                                                            >  1:2405918/1‑150 (MQ=255)

                    aGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCa                                                                                                                            >  2:4049887/1‑150 (MQ=255)

                     gcggcgCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAg                                                                                                                           <  1:1635643/150‑1 (MQ=255)

                      cggcgCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAgc                                                                                                                          <  2:3402271/150‑1 (MQ=255)

                       ggcgCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAgcg                                                                                                                         <  1:4352882/150‑1 (MQ=255)

                         cgcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCGGcgcg                                                                                                                       <  2:2234144/150‑1 (MQ=255)

                          gcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCgt                                                                                                                      >  1:837452/1‑150 (MQ=255)

                           cTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCgtg                                                                                                                     >  2:449785/1‑150 (MQ=255)

                            tGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCgtgt                                                                                                                    <  1:1655922/150‑1 (MQ=255)

                                      ggTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGAt                                                                                                          <  2:2892739/150‑1 (MQ=255)

                                       gTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATg                                                                                                         <  2:4264497/150‑1 (MQ=255)

                                        ttGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGa                                                                                                        <  1:2425808/150‑1 (MQ=255)

                                         tGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGaa                                                                                                       >  2:1155706/1‑150 (MQ=255)

                                           aaGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACa                                                                                                     <  1:3772983/150‑1 (MQ=255)

                                           aaGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACa                                                                                                     >  1:1559727/1‑150 (MQ=255)

                                                 cgcAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCt                                                                                               <  2:3114543/150‑1 (MQ=255)

                                                    aGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCTGCGCGTGGATGAAGAGATGTACTAAGCCTTTg                                                                                            >  2:3645061/1‑150 (MQ=255)

                                                      gagaTGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGt                                                                                          <  1:3222412/150‑1 (MQ=255)

                                                      gagaTGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTGGGt                                                                                          <  1:283033/150‑1 (MQ=255)

                                                       agaTGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTc                                                                                         <  2:4279529/150‑1 (MQ=255)

                                                       agaTGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTc                                                                                         <  1:415392/150‑1 (MQ=255)

                                                        gaTGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTcg                                                                                        <  2:4345451/150‑1 (MQ=255)

                                                            cAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAt                                                                                    <  1:3968173/150‑1 (MQ=255)

                                                            cAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAt                                                                                    <  2:4012263/150‑1 (MQ=255)

                                                            cAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAt                                                                                    >  1:1890115/1‑150 (MQ=255)

                                                              aaaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgt                                                                                  <  2:4223674/150‑1 (MQ=255)

                                                               aaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCCGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgtg                                                                                 >  1:3479999/1‑149 (MQ=255)

                                                                aaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgttt                                                                                >  2:1991005/1‑150 (MQ=255)

                                                                aaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgttt                                                                                >  2:1990824/1‑150 (MQ=255)

                                                                    gggTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTa                                                                            >  2:3996571/1‑150 (MQ=255)

                                                                     ggTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTAt                                                                           >  2:2348047/1‑150 (MQ=255)

                                                                        cgtcTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTc                                                                        <  2:1336723/150‑1 (MQ=255)

                                                                        cgtcTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTc                                                                        >  2:1000998/1‑150 (MQ=255)

                                                                        cgtcTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTc                                                                        >  1:2502779/1‑150 (MQ=255)

                                                                         gtcTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTACACGCACTTTGCTGCGCAAAACCCACTTAGACACCGTGCCGGTGAAACCGCCGCGCGGGTCTGAAGAGATGAACAAAGCCGTTGGTCGCGATGGTTGTTATGTca                                                                       >  2:1415620/1‑150 (MQ=255)

                                                                         gtcTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTca                                                                       >  2:1042364/1‑150 (MQ=255)

                                                                         gtcTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTca                                                                       >  2:3793322/1‑150 (MQ=255)

                                                                            tGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTcacca                                                                    <  2:2977559/150‑1 (MQ=255)

                                                                            tGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTcacca                                                                    >  1:4351600/1‑150 (MQ=255)

                                                                               cGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCAccacca                                                                 >  2:922825/1‑150 (MQ=255)

                                                                               cGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCAccacca                                                                 <  1:2397364/150‑1 (MQ=255)

                                                                                gtgtCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCAt                                                                >  1:1531841/1‑150 (MQ=255)

                                                                                gtgtCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCAt                                                                <  1:3005518/150‑1 (MQ=255)

                                                                                  gtCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTg                                                              >  2:648713/1‑150 (MQ=255)

                                                                                   tCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTgg                                                             <  2:26256/150‑1 (MQ=255)

                                                                                   tCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTgg                                                             <  2:2167907/150‑1 (MQ=255)

                                                                                    cGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGAACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGt                                                            <  1:859026/150‑1 (MQ=255)

                                                                                     gTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTc                                                           >  2:3269952/1‑150 (MQ=255)

                                                                                      tAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCt                                                          >  1:2651793/1‑150 (MQ=255)

                                                                                        aaGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGt                                                        <  1:131573/150‑1 (MQ=255)

                                                                                        aaGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGt                                                        >  2:175853/1‑150 (MQ=255)

                                                                                         aGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTc                                                       >  2:4142820/1‑150 (MQ=255)

                                                                                           aaTGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTcac                                                     >  2:3889117/1‑150 (MQ=255)

                                                                                              gggTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACaaa                                                  <  1:1772095/150‑1 (MQ=255)

                                                                                                 tCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTCTGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCg                                               >  2:1633390/1‑150 (MQ=255)

                                                                                                    ccGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTg                                            >  1:2976944/1‑150 (MQ=255)

                                                                                                        cTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGc                                        >  2:1739762/1‑150 (MQ=255)

                                                                                                          gCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGcac                                      <  2:2818251/150‑1 (MQ=255)

                                                                                                                agcGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtgc                                >  2:6944/1‑150 (MQ=255)

                                                                                                                  cGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtgctg                              <  1:2039959/150‑1 (MQ=255)

                                                                                                                  cGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtgctg                              <  1:577227/150‑1 (MQ=255)

                                                                                                                   gTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtgctgc                             >  2:4198098/1‑150 (MQ=255)

                                                                                                                    tAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCa                            >  1:3663251/1‑150 (MQ=255)

                                                                                                                    tAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCa                            <  1:2164147/150‑1 (MQ=255)

                                                                                                                    tAAACGCACTGTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCa                            <  1:4393573/150‑1 (MQ=255)

                                                                                                                     aaaCGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCAt                           <  1:81731/150‑1 (MQ=255)

                                                                                                                     aaaCGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCAt                           <  1:81528/150‑1 (MQ=255)

                                                                                                                      aaCGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATg                          <  1:2465093/150‑1 (MQ=255)

                                                                                                                      aaCGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATg                          <  1:3704917/150‑1 (MQ=255)

                                                                                                                           aCTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCttt                     >  1:4369828/1‑150 (MQ=255)

                                                                                                                            cTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTa                    >  1:2933942/1‑150 (MQ=255)

                                                                                                                            cTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTa                    >  1:3074240/1‑150 (MQ=255)

                                                                                                                            cTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTa                    >  1:1979442/1‑150 (MQ=255)

                                                                                                                             tttGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTaa                   <  1:3822064/150‑1 (MQ=255)

                                                                                                                               tgctgcGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAg                 >  1:526262/1‑150 (MQ=255)

                                                                                                                                 ctgcGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAAGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGAc               >  2:2082771/1‑150 (MQ=255)

                                                                                                                                   gcgcAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCg             <  1:1210351/150‑1 (MQ=255)

                                                                                                                                   gcgcAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACACAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCg             <  2:814195/150‑1 (MQ=255)

                                                                                                                                       aaaaCCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCAc         >  1:1018215/1‑150 (MQ=255)

                                                                                                                                        aaaCCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACt        <  2:837452/150‑1 (MQ=255)

                                                                                                                                         aaCCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTg       >  2:2282411/1‑150 (MQ=255)

                                                                                                                                          aCCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTgg      >  2:2730100/1‑150 (MQ=255)

                                                                                                                                           cccACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGa     <  2:3261197/150‑1 (MQ=255)

                                                                                                                                             cACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATc   <  2:2504243/150‑1 (MQ=255)

GGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCA  >  CP009273/530320‑530611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTG  >  CP009273/530317‑530615

CTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTA                                                                                                                                                       >  SRR19973080.3606604/1‑150 (MQ=60)

   GGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGAC                                                                                                                                                    <  SRR19973080.2991774/150‑1 (MQ=60)

      ATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAAC                                                                                                                                                 <  SRR19973080.2867955/150‑1 (MQ=60)

        TGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGT                                                                                                                                               <  SRR19973080.1675139/150‑1 (MQ=60)

         GTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTG                                                                                                                                              >  SRR19973080.552178/1‑150 (MQ=60)

         GTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTG                                                                                                                                              <  SRR19973080.2426651/150‑1 (MQ=60)

          TCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGC                                                                                                                                             <  SRR19973080.255692/150‑1 (MQ=60)

           CTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCC                                                                                                                                            <  SRR19973080.107422/150‑1 (MQ=60)

           CTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCC                                                                                                                                            <  SRR19973080.107453/150‑1 (MQ=60)

           CTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCC                                                                                                                                            >  SRR19973080.2436672/1‑150 (MQ=60)

               GTTGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTG                                                                                                                                        <  SRR19973080.2692599/150‑1 (MQ=60)

               GATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTG                                                                                                                                        >  SRR19973080.3577422/1‑150 (MQ=60)

               GATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTG                                                                                                                                        >  SRR19973080.3279153/1‑150 (MQ=60)

               GATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTG                                                                                                                                        >  SRR19973080.3279130/1‑150 (MQ=60)

               GATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTG                                                                                                                                        <  SRR19973080.2996935/150‑1 (MQ=60)

               GAGGCTACAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTG                                                                                                                                        <  SRR19973080.2692613/150‑1 (MQ=60)

                ATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGA                                                                                                                                       >  SRR19973080.2435453/1‑150 (MQ=60)

                  GCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAA                                                                                                                                     >  SRR19973080.4218746/1‑150 (MQ=60)

                   CTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAAC                                                                                                                                    >  SRR19973080.2603633/1‑150 (MQ=60)

                    TAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACC                                                                                                                                   <  SRR19973080.367820/150‑1 (MQ=60)

                    TAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACC                                                                                                                                   <  SRR19973080.3732896/150‑1 (MQ=60)

                     AAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCG                                                                                                                                  >  SRR19973080.3164651/1‑150 (MQ=60)

                     AAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCG                                                                                                                                  >  SRR19973080.2718961/1‑150 (MQ=60)

                     AAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCG                                                                                                                                  >  SRR19973080.4236887/1‑150 (MQ=60)

                     AAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCG                                                                                                                                  >  SRR19973080.4236903/1‑150 (MQ=60)

                       AGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCA                                                                                                                                >  SRR19973080.2419264/1‑150 (MQ=60)

                        GCGGCGCTGACACGGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAG                                                                                                                               <  SRR19973080.1645225/150‑1 (MQ=60)

                          GGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCG                                                                                                                             <  SRR19973080.4376642/150‑1 (MQ=60)

                             GCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGT                                                                                                                          >  SRR19973080.842490/1‑150 (MQ=60)

                               TGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGT                                                                                                                        >  SRR19973080.2543222/1‑150 (MQ=60)

                               TGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGT                                                                                                                        <  SRR19973080.1665602/150‑1 (MQ=60)

                               TGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGT                                                                                                                        >  SRR19973080.3706162/1‑150 (MQ=60)

                                GACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTA                                                                                                                       <  SRR19973080.100097/150‑1 (MQ=60)

                                GACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTA                                                                                                                       >  SRR19973080.2990666/1‑150 (MQ=60)

                                     TGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAG                                                                                                                  >  SRR19973080.3986765/1‑150 (MQ=60)

                                      GCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGA                                                                                                                 >  SRR19973080.1746406/1‑150 (MQ=60)

                                      GCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGA                                                                                                                 >  SRR19973080.1092013/1‑150 (MQ=60)

                                      GCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGGGTATGAAGA                                                                                                                 >  SRR19973080.923869/1‑150 (MQ=60)

                                       CTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAG                                                                                                                >  SRR19973080.626440/1‑150 (MQ=60)

                                           TTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGA                                                                                                            <  SRR19973080.2439269/150‑1 (MQ=60)

                                              AAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACA                                                                                                         <  SRR19973080.3793508/150‑1 (MQ=60)

                                              AAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACA                                                                                                         >  SRR19973080.1568789/1‑150 (MQ=60)

                                               AGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAA                                                                                                        >  SRR19973080.2106840/1‑150 (MQ=60)

                                                  GGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGC                                                                                                     >  SRR19973080.3855895/1‑150 (MQ=60)

                                                       AGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTG                                                                                                >  SRR19973080.1186413/1‑150 (MQ=60)

                                                         GAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGT                                                                                              <  SRR19973080.3240182/150‑1 (MQ=60)

                                                         GAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGT                                                                                              <  SRR19973080.2444839/150‑1 (MQ=60)

                                                         GAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGT                                                                                              <  SRR19973080.3240196/150‑1 (MQ=60)

                                                         GAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGT                                                                                              <  SRR19973080.437885/150‑1 (MQ=60)

                                                         GAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGT                                                                                              <  SRR19973080.14990/150‑1 (MQ=60)

                                                         GAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGT                                                                                              <  SRR19973080.285422/150‑1 (MQ=60)

                                                         GAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGT                                                                                              <  SRR19973080.1970354/150‑1 (MQ=60)

                                                         GAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTGGGT                                                                                              <  SRR19973080.284869/150‑1 (MQ=60)

                                                          AGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTC                                                                                             <  SRR19973080.417917/150‑1 (MQ=60)

                                                          AGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTC                                                                                             <  SRR19973080.417915/150‑1 (MQ=60)

                                                               CAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAT                                                                                        >  SRR19973080.1900670/1‑150 (MQ=60)

                                                               CAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAT                                                                                        <  SRR19973080.3990014/150‑1 (MQ=60)

                                                               CAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAT                                                                                        <  SRR19973080.2438629/150‑1 (MQ=60)

                                                                AAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATG                                                                                       >  SRR19973080.3214352/1‑150 (MQ=60)

                                                                  AAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCCGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTG                                                                                     >  SRR19973080.3499148/1‑150 (MQ=60)

                                                                    AGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTG                                                                                   >  SRR19973080.25091/1‑150 (MQ=60)

                                                                    AGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAAAGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTG                                                                                   >  SRR19973080.25062/1‑150 (MQ=60)

                                                                       GGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTA                                                                                >  SRR19973080.4382681/1‑150 (MQ=60)

                                                                           CGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTC                                                                            >  SRR19973080.2516592/1‑150 (MQ=60)

                                                                             TCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCAC                                                                          <  SRR19973080.1630404/150‑1 (MQ=60)

                                                                             TCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCAC                                                                          <  SRR19973080.1630454/150‑1 (MQ=60)

                                                                               TGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCA                                                                        >  SRR19973080.4375353/1‑150 (MQ=60)

                                                                                GCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCAC                                                                       <  SRR19973080.259776/150‑1 (MQ=60)

                                                                                  CGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCA                                                                     <  SRR19973080.2410678/150‑1 (MQ=60)

                                                                                   GTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCAT                                                                    >  SRR19973080.1540664/1‑150 (MQ=60)

                                                                                   GTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCAT                                                                    >  SRR19973080.1540807/1‑150 (MQ=60)

                                                                                   GTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCAT                                                                    <  SRR19973080.3021685/150‑1 (MQ=60)

                                                                                    TGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATT                                                                   <  SRR19973080.247447/150‑1 (MQ=60)

                                                                                       CGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGAACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGT                                                                <  SRR19973080.864252/150‑1 (MQ=60)

                                                                                        GTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTC                                                               >  SRR19973080.3772775/1‑150 (MQ=60)

                                                                                         TAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCT                                                              >  SRR19973080.2666116/1‑150 (MQ=60)

                                                                                          AAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTG                                                             >  SRR19973080.2918345/1‑150 (MQ=60)

                                                                                           AAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGT                                                            <  SRR19973080.132682/150‑1 (MQ=60)

                                                                                           AAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGT                                                            <  SRR19973080.2411957/150‑1 (MQ=60)

                                                                                              AATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCAC                                                         >  SRR19973080.2833762/1‑150 (MQ=60)

                                                                                                 GGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAA                                                      <  SRR19973080.1782260/150‑1 (MQ=60)

                                                                                                 GGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAA                                                      <  SRR19973080.1782389/150‑1 (MQ=60)

                                                                                                      GCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCT                                                 <  SRR19973080.3167004/150‑1 (MQ=60)

                                                                                                       CCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTG                                                >  SRR19973080.2993024/1‑150 (MQ=60)

                                                                                                             GCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCAC                                          >  SRR19973080.4381623/1‑150 (MQ=60)

                                                                                                                  CAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATAGGTCTGTCACAAATCGCTGCGGCACAAATG                                     >  SRR19973080.4545989/1‑150 (MQ=60)

                                                                                                                  CAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGAGAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATG                                     <  SRR19973080.166716/150‑1 (MQ=60)

                                                                                                                  CAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATG                                     <  SRR19973080.166609/150‑1 (MQ=60)

                                                                                                                  CAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATG                                     <  SRR19973080.1970989/150‑1 (MQ=60)

                                                                                                                     CGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTG                                  <  SRR19973080.2051647/150‑1 (MQ=60)

                                                                                                                     CGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTG                                  <  SRR19973080.2051544/150‑1 (MQ=60)

                                                                                                                     CGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTG                                  <  SRR19973080.580833/150‑1 (MQ=60)

                                                                                                                     CGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTG                                  >  SRR19973080.403181/1‑150 (MQ=60)

                                                                                                                       TAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCA                                <  SRR19973080.2176236/150‑1 (MQ=60)

                                                                                                                       TAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCA                                <  SRR19973080.1767208/150‑1 (MQ=60)

                                                                                                                       TAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCA                                >  SRR19973080.3683441/1‑150 (MQ=60)

                                                                                                                       TAAACGCACTGTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCA                                <  SRR19973080.4417496/150‑1 (MQ=60)

                                                                                                                        AAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCAT                               <  SRR19973080.82306/150‑1 (MQ=60)

                                                                                                                        AAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCAT                               <  SRR19973080.82512/150‑1 (MQ=60)

                                                                                                                        AAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCAT                               <  SRR19973080.82475/150‑1 (MQ=60)

                                                                                                                        AAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCAT                               <  SRR19973080.82382/150‑1 (MQ=60)

                                                                                                                        AAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCAT                               >  SRR19973080.2402061/1‑150 (MQ=60)

                                                                                                                         AACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATG                              <  SRR19973080.2478735/150‑1 (MQ=60)

                                                                                                                         AACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATG                              <  SRR19973080.3725254/150‑1 (MQ=60)

                                                                                                                           CGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTC                            >  SRR19973080.514538/1‑150 (MQ=60)

                                                                                                                            GCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCT                           >  SRR19973080.3466622/1‑150 (MQ=60)

                                                                                                                              ACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTT                         >  SRR19973080.4393662/1‑150 (MQ=60)

                                                                                                                              ACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTT                         <  SRR19973080.839661/150‑1 (MQ=60)

                                                                                                                              ACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTT                         >  SRR19973080.4395520/1‑150 (MQ=60)

                                                                                                                              ACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTT                         <  SRR19973080.3114613/150‑1 (MQ=60)

                                                                                                                               CTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTA                        >  SRR19973080.2949890/1‑150 (MQ=60)

                                                                                                                               CTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTA                        >  SRR19973080.3090993/1‑150 (MQ=60)

                                                                                                                               CTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTA                        >  SRR19973080.3090902/1‑150 (MQ=60)

                                                                                                                               CTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTA                        >  SRR19973080.1991656/1‑150 (MQ=60)

                                                                                                                               CTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTA                        >  SRR19973080.1990678/1‑150 (MQ=60)

                                                                                                                                TTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGTTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCTCTGCGGCACAAATGCTGCATGTCTTTAA                       >  SRR19973080.3087627/1‑150 (MQ=60)

                                                                                                                                TTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAA                       <  SRR19973080.3843001/150‑1 (MQ=60)

                                                                                                                                TTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAA                       <  SRR19973080.3842970/150‑1 (MQ=60)

                                                                                                                                  TGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAG                     <  SRR19973080.4077255/150‑1 (MQ=60)

                                                                                                                                  TGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAG                     >  SRR19973080.529613/1‑150 (MQ=60)

                                                                                                                                   GCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGA                    <  SRR19973080.535874/150‑1 (MQ=60)

                                                                                                                                    CTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGAC                   <  SRR19973080.1666718/150‑1 (MQ=60)

                                                                                                                                      GCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCG                 <  SRR19973080.1217664/150‑1 (MQ=60)

                                                                                                                                       CGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGC                >  SRR19973080.853464/1‑150 (MQ=60)

                                                                                                                                          AAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCAC             >  SRR19973080.1024284/1‑150 (MQ=60)

                                                                                                                                          AAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCAC             >  SRR19973080.14155/1‑150 (MQ=60)

                                                                                                                                           AAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACT            >  SRR19973080.1290265/1‑150 (MQ=60)

                                                                                                                                             ACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGG          >  SRR19973080.2943457/1‑150 (MQ=60)

                                                                                                                                                 ACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCA      >  SRR19973080.2281905/1‑150 (MQ=60)

                                                                                                                                                 ACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCA      >  SRR19973080.855343/1‑150 (MQ=60)

                                                                                                                                                     AGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTG  <  SRR19973080.4520347/150‑1 (MQ=60)

CTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTG  >  CP009273/530317‑530615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`