Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 530,466 C→A 100% F365L (TTC→TTA)  gcl → glyoxylate carboligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273530,4660CA100.0% 563.3 / ‑6.7 168F365L (TTC→TTA) gclglyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (74/93);  minor base T (0/1);  total (74/94)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 168 total aligned reads displayed.

GTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATC  >  CP009273/530321‑530610
                                                                                                                                                 |                                                                                                                                                
gTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGa                                                                                                                                                <  2:3063689/148‑1 (MQ=255)
 tATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGAc                                                                                                                                               >  1:2622101/1‑148 (MQ=255)
         ctGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGcc                                                                                                                                       >  1:792359/1‑148 (MQ=255)
           gATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCgg                                                                                                                                     <  1:337546/148‑1 (MQ=255)
           gATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCgg                                                                                                                                     <  1:337547/148‑1 (MQ=255)
           gATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCgg                                                                                                                                     <  1:1445465/148‑1 (MQ=255)
            aTGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGt                                                                                                                                    <  2:851722/148‑1 (MQ=255)
            aTGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGt                                                                                                                                    <  2:2507872/148‑1 (MQ=255)
            aTGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGt                                                                                                                                    >  2:626210/1‑148 (MQ=255)
            aTGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGt                                                                                                                                    >  2:2998558/1‑148 (MQ=255)
               cTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACAGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGaa                                                                                                                                 >  1:676854/1‑148 (MQ=255)
                 aaaGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAAc                                                                                                                               >  2:1719240/1‑148 (MQ=255)
                 aaaGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAAc                                                                                                                               <  2:2917439/148‑1 (MQ=255)
                 aaaGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAAc                                                                                                                               <  2:2917341/148‑1 (MQ=255)
                  aaGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAAcc                                                                                                                              >  2:1076136/1‑148 (MQ=255)
                   aGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCg                                                                                                                             >  2:1854930/1‑148 (MQ=255)
                        cgcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAgcg                                                                                                                        <  2:3119505/148‑1 (MQ=255)
                            gACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCgtg                                                                                                                    <  2:2125403/148‑1 (MQ=255)
                             acacTGCTGTTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACTCACTTTGCTGCGCAAAAACCACTTAGACAACGTGCCGGTGAAACCGCAGCGCgtgt                                                                                                                   <  2:2393459/148‑1 (MQ=255)
                             acacTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCgtgt                                                                                                                   >  1:1639837/1‑148 (MQ=255)
                              cacTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTa                                                                                                                  >  2:1439407/1‑148 (MQ=255)
                              cacTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTa                                                                                                                  >  2:2700839/1‑148 (MQ=255)
                                  gctgGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGaa                                                                                                              >  1:2118047/1‑148 (MQ=255)
                                    tgGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAaga                                                                                                            <  2:1218090/148‑1 (MQ=255)
                                       ttGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGAt                                                                                                         <  2:2463705/148‑1 (MQ=255)
                                        tGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATg                                                                                                        <  2:3166568/148‑1 (MQ=255)
                                        tGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATg                                                                                                        <  2:2627845/148‑1 (MQ=255)
                                        tGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATg                                                                                                        >  1:1567713/1‑148 (MQ=255)
                                        tGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATg                                                                                                        <  2:1526095/148‑1 (MQ=255)
                                        tGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATg                                                                                                        >  1:1569046/1‑148 (MQ=255)
                                          aaGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGaa                                                                                                      <  2:601014/148‑1 (MQ=255)
                                             tGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACaa                                                                                                   <  1:996175/148‑1 (MQ=255)
                                             tGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACaa                                                                                                   <  1:2652258/148‑1 (MQ=255)
                                             tGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACaa                                                                                                   <  2:197074/148‑1 (MQ=255)
                                              ggCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACaaa                                                                                                  <  2:1324063/148‑1 (MQ=255)
                                              ggCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACaaa                                                                                                  <  2:210016/148‑1 (MQ=255)
                                              ggCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACaaa                                                                                                  <  2:209917/148‑1 (MQ=255)
                                                    ggAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCttt                                                                                            >  1:964180/1‑148 (MQ=255)
                                                     gagaTGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTg                                                                                           >  2:725148/1‑148 (MQ=255)
                                                     gagaTGCAAAAAGCAGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTg                                                                                           >  2:743553/1‑148 (MQ=255)
                                                         tGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTcg                                                                                       <  2:137336/148‑1 (MQ=255)
                                                            aaaaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGa                                                                                    <  1:3084107/148‑1 (MQ=255)
                                                            aaaaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGa                                                                                    <  1:3084104/148‑1 (MQ=255)
                                                            aaaaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGa                                                                                    <  2:1353874/148‑1 (MQ=255)
                                                            aaaaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGa                                                                                    >  2:750238/1‑148 (MQ=255)
                                                              aaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtg                                                                                  <  2:2274696/148‑1 (MQ=255)
                                                              aaaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtg                                                                                  >  1:304189/1‑148 (MQ=255)
                                                               aaGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAggt                                                                                 >  2:1403966/1‑148 (MQ=255)
                                                                aGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgtt                                                                                >  2:88163/1‑148 (MQ=255)
                                                                 gCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgttt                                                                               >  1:2637379/1‑148 (MQ=255)
                                                                  cGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgtttg                                                                              <  2:2184570/148‑1 (MQ=255)
                                                                   gggTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGAtgtttgt                                                                             <  1:973721/148‑1 (MQ=255)
                                                                          cTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTca                                                                      >  2:2766837/1‑148 (MQ=255)
                                                                            gCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTcacc                                                                    >  1:303159/1‑148 (MQ=255)
                                                                             ccGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTcacca                                                                   <  2:1143159/148‑1 (MQ=255)
                                                                                tgtCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCAccacca                                                                >  1:251698/1‑148 (MQ=255)
                                                                                tgtCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCAccacca                                                                >  1:2839446/1‑148 (MQ=255)
                                                                                     tAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGt                                                           >  2:2501647/1‑148 (MQ=255)
                                                                                     tAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGt                                                           <  1:1411225/148‑1 (MQ=255)
                                                                                     tAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGt                                                           <  2:360715/148‑1 (MQ=255)
                                                                                       aaGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCt                                                         >  2:60203/1‑148 (MQ=255)
                                                                                         gAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGt                                                       <  1:3198695/148‑1 (MQ=255)
                                                                                          aaTGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGTAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTc                                                      <  2:1842165/148‑1 (MQ=255)
                                                                                          aaTGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTc                                                      >  1:431301/1‑148 (MQ=255)
                                                                                          aaTGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATCGGTCTGTc                                                      >  2:135242/1‑148 (MQ=255)
                                                                                             gggTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTcaca                                                   >  2:1749352/1‑148 (MQ=255)
                                                                                              ggTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACaa                                                  <  1:2811896/148‑1 (MQ=255)
                                                                                              ggTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACaa                                                  >  2:1561983/1‑148 (MQ=255)
                                                                                                tCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAAt                                                <  1:1189267/148‑1 (MQ=255)
                                                                                                   ccGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGc                                             <  1:455977/148‑1 (MQ=255)
                                                                                                     gACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTg                                           <  2:412999/148‑1 (MQ=255)
                                                                                                         gCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGc                                       >  2:732530/1‑148 (MQ=255)
                                                                                                              cagcGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACaaa                                  >  2:2380784/1‑148 (MQ=255)
                                                                                                                gcGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtg                                <  2:2722235/148‑1 (MQ=255)
                                                                                                                gcGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtg                                <  2:925492/148‑1 (MQ=255)
                                                                                                                gcGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtg                                <  2:2306061/148‑1 (MQ=255)
                                                                                                                gcGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtg                                >  1:2179970/1‑148 (MQ=255)
                                                                                                                 cGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtgc                               <  1:124399/148‑1 (MQ=255)
                                                                                                                    aaaCGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAAtgctgc                            <  2:1795344/148‑1 (MQ=255)
                                                                                                                        gCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGt                        <  1:1099810/148‑1 (MQ=255)
                                                                                                                        gCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGt                        <  2:1434468/148‑1 (MQ=255)
                                                                                                                         cACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATTAACAAAGCCTTTGGTCGCGATGTTTTTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTc                       <  1:1792123/148‑1 (MQ=255)
                                                                                                                         cACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAATCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTc                       >  1:3032888/1‑148 (MQ=255)
                                                                                                                          aCTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCt                      <  1:2538217/148‑1 (MQ=255)
                                                                                                                          aCTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCt                      <  1:2538241/148‑1 (MQ=255)
                                                                                                                             ttGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTa                   >  2:256849/1‑148 (MQ=255)
                                                                                                                                ctgcGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAg                <  1:743442/148‑1 (MQ=255)
                                                                                                                                ctgcGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAg                <  1:743553/148‑1 (MQ=255)
                                                                                                                                ctgcGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAg                <  2:1702200/148‑1 (MQ=255)
                                                                                                                                 tgcGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGa               <  2:1202834/148‑1 (MQ=255)
                                                                                                                                 tgcGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGa               <  2:1202873/148‑1 (MQ=255)
                                                                                                                                       aaaCCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCa         >  2:1431392/1‑148 (MQ=255)
                                                                                                                                         aCCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACt       >  1:1771755/1‑148 (MQ=255)
                                                                                                                                           ccACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTgg     >  2:673817/1‑148 (MQ=255)
                                                                                                                                           ccACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTgg     >  1:1031484/1‑148 (MQ=255)
                                                                                                                                            cACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGa    <  1:626210/148‑1 (MQ=255)
                                                                                                                                            cACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGa    <  1:2287583/148‑1 (MQ=255)
                                                                                                                                            cACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGa    <  1:1076136/148‑1 (MQ=255)
                                                                                                                                             aCTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGAt   <  1:1433409/148‑1 (MQ=255)
                                                                                                                                             aCTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGAt   >  1:1181610/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATC  >  CP009273/530321‑530610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACT  >  CP009273/530317‑530614
                                                                                                                                                     |                                                                                                                                                    
CTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTA                                                                                                                                                      <  SRR19973070.1622106/150‑1 (MQ=60)
CTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTA                                                                                                                                                      <  SRR19973070.1622093/150‑1 (MQ=60)
   GGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGAC                                                                                                                                                   >  SRR19973070.2639205/1‑150 (MQ=60)
           CTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCC                                                                                                                                           >  SRR19973070.797843/1‑150 (MQ=60)
               GATGCTAACGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTG                                                                                                                                       <  SRR19973070.340010/150‑1 (MQ=60)
               GATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTG                                                                                                                                       <  SRR19973070.1455506/150‑1 (MQ=60)
               GATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTG                                                                                                                                       <  SRR19973070.340011/150‑1 (MQ=60)
                 TGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACAGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAA                                                                                                                                     >  SRR19973070.681681/1‑150 (MQ=60)
                              CTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTG                                                                                                                        >  SRR19973070.1189092/1‑150 (MQ=60)
                               TGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGT                                                                                                                       >  SRR19973070.1646675/1‑150 (MQ=60)
                               TGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGT                                                                                                                       >  SRR19973070.1651187/1‑150 (MQ=60)
                                    CTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAA                                                                                                                  >  SRR19973070.2132752/1‑150 (MQ=60)
                                    CTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAA                                                                                                                  >  SRR19973070.2132241/1‑150 (MQ=60)
                                          GTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATG                                                                                                            >  SRR19973070.1578739/1‑150 (MQ=60)
                                          GTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATG                                                                                                            >  SRR19973070.1580075/1‑150 (MQ=60)
                                                 TGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAG                                                                                                     <  SRR19973070.1003279/150‑1 (MQ=60)
                                                 TGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAG                                                                                                     <  SRR19973070.2669516/150‑1 (MQ=60)
                                                 TGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAG                                                                                                     <  SRR19973070.2669952/150‑1 (MQ=60)
                                                    CGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCT                                                                                                  <  SRR19973070.1504393/150‑1 (MQ=60)
                                                      CAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCCGCGCGTGTATGAAGAGATGAACAAAGCCTTT                                                                                                >  SRR19973070.1447278/1‑150 (MQ=60)
                                                      CAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTT                                                                                                >  SRR19973070.971067/1‑150 (MQ=60)
                                                        GGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGG                                                                                              <  SRR19973070.3105256/150‑1 (MQ=60)
                                                            ATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGC                                                                                          >  SRR19973070.3035336/1‑150 (MQ=60)
                                                                AAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATG                                                                                      <  SRR19973070.3103940/150‑1 (MQ=60)
                                                                AAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATG                                                                                      <  SRR19973070.3103937/150‑1 (MQ=60)
                                                                AAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATG                                                                                      <  SRR19973070.3103926/150‑1 (MQ=60)
                                                                AAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATG                                                                                      >  SRR19973070.306338/1‑150 (MQ=60)
                                                                AAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATG                                                                                      >  SRR19973070.305775/1‑150 (MQ=60)
                                                                 AAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGT                                                                                     <  SRR19973070.175389/150‑1 (MQ=60)
                                                                   AAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTT                                                                                   >  SRR19973070.2654570/1‑150 (MQ=60)
                                                                       GGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTA                                                                               <  SRR19973070.980842/150‑1 (MQ=60)
                                                                       GGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTA                                                                               <  SRR19973070.980665/150‑1 (MQ=60)
                                                                           CGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTC                                                                           <  SRR19973070.1093232/150‑1 (MQ=60)
                                                                              CTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACC                                                                        >  SRR19973070.305294/1‑150 (MQ=60)
                                                                                  CGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCA                                                                    >  SRR19973070.2857826/1‑150 (MQ=60)
                                                                                  CGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCA                                                                    >  SRR19973070.253410/1‑150 (MQ=60)
                                                                                         TAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCT                                                             <  SRR19973070.1420852/150‑1 (MQ=60)
                                                                                            AGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTC                                                          >  SRR19973070.434437/1‑150 (MQ=60)
                                                                                            AGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTC                                                          >  SRR19973070.435200/1‑150 (MQ=60)
                                                                                             GAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCA                                                         <  SRR19973070.3219397/150‑1 (MQ=60)
                                                                                              AATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCAC                                                        <  SRR19973070.421074/150‑1 (MQ=60)
                                                                                                  GGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAAT                                                    <  SRR19973070.3317870/150‑1 (MQ=60)
                                                                                                  GGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAAT                                                    <  SRR19973070.2830100/150‑1 (MQ=60)
                                                                                                  GGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAAT                                                    <  SRR19973070.2830080/150‑1 (MQ=60)
                                                                                                    TCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCG                                                  <  SRR19973070.1197628/150‑1 (MQ=60)
                                                                                                       CCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTG                                               <  SRR19973070.459247/150‑1 (MQ=60)
                                                                                                             GCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCAC                                         <  SRR19973070.760080/150‑1 (MQ=60)
                                                                                                                  CAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATG                                    >  SRR19973070.2194398/1‑150 (MQ=60)
                                                                                                                    GCGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCT                                  <  SRR19973070.2586330/150‑1 (MQ=60)
                                                                                                                     CGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTG                                 <  SRR19973070.125570/150‑1 (MQ=60)
                                                                                                                     CGTAAACGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTG                                 <  SRR19973070.125501/150‑1 (MQ=60)
                                                                                                                           CGCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAATCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTC                           >  SRR19973070.3052184/1‑150 (MQ=60)
                                                                                                                            GCACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCT                          <  SRR19973070.1107371/150‑1 (MQ=60)
                                                                                                                             CACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATTAACAAAGCCTTTGGTCGCGATGTTTTTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTT                         <  SRR19973070.1804505/150‑1 (MQ=60)
                                                                                                                             CACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTT                         <  SRR19973070.826909/150‑1 (MQ=60)
                                                                                                                              ACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTT                        <  SRR19973070.2554830/150‑1 (MQ=60)
                                                                                                                              ACTTTGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTT                        <  SRR19973070.2554806/150‑1 (MQ=60)
                                                                                                                                  TGCTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAG                    <  SRR19973070.2545286/150‑1 (MQ=60)
                                                                                                                                    CTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGAC                  <  SRR19973070.748809/150‑1 (MQ=60)
                                                                                                                                    CTGCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGAC                  <  SRR19973070.748697/150‑1 (MQ=60)
                                                                                                                                      GCGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCG                <  SRR19973070.687527/150‑1 (MQ=60)
                                                                                                                                       CGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGC               >  SRR19973070.2508907/1‑150 (MQ=60)
                                                                                                                                       CGCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGC               >  SRR19973070.2028138/1‑150 (MQ=60)
                                                                                                                                        GCAAAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCC              <  SRR19973070.1204733/150‑1 (MQ=60)
                                                                                                                                           AAACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACT           >  SRR19973070.1784019/1‑150 (MQ=60)
                                                                                                                                            AACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTG          >  SRR19973070.2641043/1‑150 (MQ=60)
                                                                                                                                             ACCCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGG         >  SRR19973070.1038782/1‑150 (MQ=60)
                                                                                                                                               CCACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGAT       >  SRR19973070.1189912/1‑150 (MQ=60)
                                                                                                                                                CACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATC      <  SRR19973070.630638/150‑1 (MQ=60)
                                                                                                                                                CACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATC      <  SRR19973070.630721/150‑1 (MQ=60)
                                                                                                                                                CACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATC      <  SRR19973070.2302796/150‑1 (MQ=60)
                                                                                                                                                CACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATC      <  SRR19973070.1083634/150‑1 (MQ=60)
                                                                                                                                                 ACTTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCA     <  SRR19973070.1443320/150‑1 (MQ=60)
                                                                                                                                                   TTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAAC   <  SRR19973070.1085426/150‑1 (MQ=60)
                                                                                                                                                   TTAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAAC   <  SRR19973070.1084485/150‑1 (MQ=60)
                                                                                                                                                    TAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACT  >  SRR19973070.485559/1‑150 (MQ=60)
                                                                                                                                                    TAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACT  >  SRR19973070.1182998/1‑150 (MQ=60)
                                                                                                                                                    TAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACT  >  SRR19973070.1305188/1‑150 (MQ=60)
                                                                                                                                                    TAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACT  <  SRR19973070.1731130/150‑1 (MQ=60)
                                                                                                                                                    TAGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACT  >  SRR19973070.871843/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCGATGTTTGTTATGTCACCACCATTGGTCTGTCACAAATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACT  >  CP009273/530317‑530614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑