Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 546,491 G→T 89.9% V199V (GTG→GTT)  ybcF → putative carbonate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273546,4910GT89.9% 620.7 / 55.1 213V199V (GTG→GTT) ybcFputative carbonate kinase
Reads supporting (aligned to +/- strand):  ref base G (16/6);  new base T (96/95);  total (112/101)
Fisher's exact test for biased strand distribution p-value = 6.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGC  >  CP009273/546343‑546637
                                                                                                                                                    |                                                                                                                                                  
atTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGtga                                                                                                                                                   >  1:737581/1‑150 (MQ=255)
 tttGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCACAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGCGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttat                                                                                                                                                  <  2:890645/150‑1 (MQ=255)
 tttGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttat                                                                                                                                                  <  2:2354286/150‑1 (MQ=255)
  ttGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttatt                                                                                                                                                 <  1:4462473/150‑1 (MQ=255)
       ccGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATaa                                                                                                                                            >  2:197083/1‑150 (MQ=255)
       ccGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATaa                                                                                                                                            >  2:1008664/1‑150 (MQ=255)
       ccGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATaa                                                                                                                                            >  2:1761954/1‑150 (MQ=255)
          ggtggtGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGa                                                                                                                                         <  1:3114763/150‑1 (MQ=255)
            tggtggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGAtc                                                                                                                                       >  1:3670721/1‑150 (MQ=255)
             ggtggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGAtct                                                                                                                                      >  2:2814196/1‑150 (MQ=255)
              gtggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGAtctc                                                                                                                                     <  2:2299851/150‑1 (MQ=255)
               tggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcg                                                                                                                                    >  2:3800106/1‑150 (MQ=255)
                ggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTcgc                                                                                                                                   >  2:1492495/1‑150 (MQ=255)
                   gTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAATAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTAGTGATAAAGATCTcgccgc                                                                                                                                >  2:1941572/1‑150 (MQ=255)
                    tctcCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCt                                                                                                                               <  2:2567967/150‑1 (MQ=255)
                     ctcCGCAACCGCGAAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGAGGTGATTTGCAGTGGCGGCGGCTGAGTGCCTGAGACGGATGACGGAGCAGCGAGTGAAGCAGTGATTGATAAAGATCTCGCCTCTg                                                                                                                              >  1:2595525/1‑150 (MQ=255)
                         gCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGtt                                                                                                                          <  1:4525007/150‑1 (MQ=255)
                          cAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTg                                                                                                                         <  2:2231010/150‑1 (MQ=255)
                          cAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTg                                                                                                                         <  1:3758226/150‑1 (MQ=255)
                           aaCCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGTGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGc                                                                                                                        <  1:3068375/150‑1 (MQ=255)
                           aaCCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGc                                                                                                                        >  1:2587434/1‑150 (MQ=255)
                           aaCCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGc                                                                                                                        >  2:2388642/1‑150 (MQ=255)
                              cgcgTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATATCGCCGCTGCGTTGCTCg                                                                                                                     >  1:3458183/1‑150 (MQ=255)
                              cgcgTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCg                                                                                                                     >  1:1120325/1‑150 (MQ=255)
                                 gTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGTATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCg                                                                                                                  <  2:149559/150‑1 (MQ=255)
                                  tAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGa                                                                                                                 >  2:3494429/1‑150 (MQ=255)
                                   aaaaTTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAg                                                                                                                >  1:4163310/1‑150 (MQ=255)
                                     aaTTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCa                                                                                                              <  1:3947659/150‑1 (MQ=255)
                                      aTTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAg                                                                                                             >  2:3277894/1‑150 (MQ=255)
                                          tcGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTa                                                                                                         >  1:2840525/1‑150 (MQ=255)
                                             aCAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATg                                                                                                      <  2:486763/150‑1 (MQ=255)
                                               aGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCa                                                                                                    >  2:1160509/1‑150 (MQ=255)
                                                gCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAg                                                                                                   <  1:2957985/150‑1 (MQ=255)
                                                   aaGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATg                                                                                                <  1:404578/150‑1 (MQ=255)
                                                       cATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACt                                                                                            >  1:2942333/1‑150 (MQ=255)
                                                         tCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGATGCTCGCCGAGCAGATTAATGCAGATGGACtcg                                                                                          >  2:4435364/1‑148 (MQ=255)
                                                          cGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGt                                                                                         >  2:1141242/1‑150 (MQ=255)
                                                          cGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGt                                                                                         >  2:2985544/1‑150 (MQ=255)
                                                          cGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGt                                                                                         >  2:2555763/1‑150 (MQ=255)
                                                          cGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGt                                                                                         >  2:1664697/1‑150 (MQ=255)
                                                           gAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTg                                                                                        <  2:1729606/150‑1 (MQ=255)
                                                            aGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGa                                                                                       >  1:3994454/1‑150 (MQ=255)
                                                               tgttgCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATcc                                                                                    <  2:1772919/150‑1 (MQ=255)
                                                                gttgCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCt                                                                                   >  1:2221624/1‑150 (MQ=255)
                                                                gttgCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCt                                                                                   >  1:3338132/1‑150 (MQ=255)
                                                                 ttgCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTc                                                                                  <  1:4449726/150‑1 (MQ=255)
                                                                 ttgCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTc                                                                                  <  2:3854614/150‑1 (MQ=255)
                                                                    cTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCAcc                                                                               <  1:146593/150‑1 (MQ=255)
                                                                      cAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCAccta                                                                             <  1:2060945/150‑3 (MQ=255)
                                                                      cAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGa                                                                             >  1:2363359/1‑150 (MQ=255)
                                                                               gCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGc                                                                    >  2:260303/1‑150 (MQ=255)
                                                                                   gtggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGta                                                                <  1:3152892/150‑1 (MQ=255)
                                                                                   gtggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGta                                                                <  1:1793320/150‑1 (MQ=255)
                                                                                    tggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGtat                                                               >  1:866052/1‑150 (MQ=255)
                                                                                    tggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGtat                                                               >  2:1127197/1‑150 (MQ=255)
                                                                                    tggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGtat                                                               >  2:1569440/1‑150 (MQ=255)
                                                                                       tgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATg                                                            >  1:443020/1‑150 (MQ=255)
                                                                                         aTTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGaa                                                          <  2:3593288/150‑1 (MQ=255)
                                                                                            tGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAAc                                                       <  2:1521930/150‑1 (MQ=255)
                                                                                               aGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgg                                                    <  2:2345478/150‑1 (MQ=255)
                                                                                                 tGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgggg                                                  >  1:359204/1‑150 (MQ=255)
                                                                                                 tGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgggg                                                  <  2:1401826/150‑1 (MQ=255)
                                                                                                   gcggcggcgGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGaa                                                <  2:3373742/150‑1 (MQ=255)
                                                                                                   gcggcggcgGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGaa                                                <  2:3769007/150‑1 (MQ=255)
                                                                                                    cggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAc                                               <  2:1893545/150‑1 (MQ=255)
                                                                                                     ggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcg                                              <  2:162491/150‑1 (MQ=255)
                                                                                                     ggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcg                                              >  2:1266284/1‑150 (MQ=255)
                                                                                                     ggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcg                                              <  1:905190/150‑1 (MQ=255)
                                                                                                        ggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcgccg                                           <  1:1008664/150‑1 (MQ=255)
                                                                                                        ggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcgccg                                           >  1:3864749/1‑150 (MQ=255)
                                                                                                           ggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCgcag                                        >  1:3861839/1‑150 (MQ=255)
                                                                                                           ggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCgcag                                        >  2:2624057/1‑150 (MQ=255)
                                                                                                             tgtgCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCgcagca                                      >  2:2770153/1‑150 (MQ=255)
                                                                                                             tgtgCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCgcagca                                      <  1:1182873/150‑1 (MQ=255)
                                                                                                                gCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACg                                   <  2:3731295/150‑1 (MQ=255)
                                                                                                                 ccTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGt                                  >  2:826050/1‑150 (MQ=255)
                                                                                                                 ccTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGt                                  >  1:2758830/1‑150 (MQ=255)
                                                                                                                  cTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTg                                 <  1:1279526/150‑1 (MQ=255)
                                                                                                                     tgacggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCa                              >  2:3867745/1‑150 (MQ=255)
                                                                                                                       acggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCAtt                            <  1:409641/150‑1 (MQ=255)
                                                                                                                         ggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCg                          >  1:320155/1‑150 (MQ=255)
                                                                                                                         ggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCg                          <  2:862982/150‑1 (MQ=255)
                                                                                                                            tgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCa                       >  1:2701483/1‑150 (MQ=255)
                                                                                                                             gacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCAt                      <  1:4374418/150‑1 (MQ=255)
                                                                                                                             gacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCAt                      <  1:61503/150‑1 (MQ=255)
                                                                                                                                ggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGcc                   >  2:3341107/1‑150 (MQ=255)
                                                                                                                                ggaGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGcc                   >  2:3661977/1‑150 (MQ=255)
                                                                                                                                 gaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCca                  <  2:428335/150‑1 (MQ=255)
                                                                                                                                  aGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCcac                 >  2:4059850/1‑150 (MQ=255)
                                                                                                                                  aGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCcac                 >  2:4343630/1‑150 (MQ=255)
                                                                                                                                    cAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCacac               >  2:1538312/1‑150 (MQ=255)
                                                                                                                                      gggAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCg             >  2:745057/1‑150 (MQ=255)
                                                                                                                                      gggAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCg             <  1:2906908/150‑1 (MQ=255)
                                                                                                                                       ggAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCgg            <  2:121204/150‑1 (MQ=255)
                                                                                                                                            gAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAg       >  1:1546463/1‑150 (MQ=255)
                                                                                                                                              aGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGtt     <  1:1866421/150‑1 (MQ=255)
                                                                                                                                              aGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGtt     <  1:2012231/150‑1 (MQ=255)
                                                                                                                                                cAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAg   >  2:3038102/1‑150 (MQ=255)
                                                                                                                                                 aGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAgc  >  1:51792/1‑150 (MQ=255)
                                                                                                                                                 aGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAgc  >  1:3566337/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGC  >  CP009273/546343‑546637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCG  >  CP009273/546342‑546638
                                                                                                                                                     |                                                                                                                                                   
TATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTT                                                                                                                                                     >  SRR19973080.975132/1‑150 (MQ=60)
TATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTT                                                                                                                                                     >  SRR19973080.975099/1‑150 (MQ=60)
TATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTT                                                                                                                                                     >  SRR19973080.3638392/1‑150 (MQ=60)
TATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTT                                                                                                                                                     <  SRR19973080.2347849/150‑1 (MQ=60)
 ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGA                                                                                                                                                    >  SRR19973080.741789/1‑150 (MQ=60)
   TTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATT                                                                                                                                                  <  SRR19973080.4486690/150‑1 (MQ=60)
   TTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATT                                                                                                                                                  <  SRR19973080.4486655/150‑1 (MQ=60)
      CGCCGGGTGGTGTCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATAGAGTTGTTTCTCACAGAGGGGCATGTGGTGATTTTCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGAT                                                                                                                                               <  SRR19973080.111302/150‑1 (MQ=60)
      CGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGAT                                                                                                                                               >  SRR19973080.3686199/1‑150 (MQ=60)
         CGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAA                                                                                                                                            >  SRR19973080.3309194/1‑150 (MQ=60)
           GGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGA                                                                                                                                          <  SRR19973080.3131883/150‑1 (MQ=60)
            GTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGAT                                                                                                                                         <  SRR19973080.1831483/150‑1 (MQ=60)
             TGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATC                                                                                                                                        >  SRR19973080.3690936/1‑150 (MQ=60)
                     TCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCT                                                                                                                                >  SRR19973080.4023487/1‑150 (MQ=60)
                      CTCCGCAACCGCGAAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGAGGTGATTTGCAGTGGCGGCGGCTGAGTGCCTGAGACGGATGACGGAGCAGCGAGTGAAGCAGTGATTGATAAAGATCTCGCCTCTG                                                                                                                               >  SRR19973080.2609649/1‑150 (MQ=60)
                          GCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTT                                                                                                                           <  SRR19973080.4549383/150‑1 (MQ=60)
                          GCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTT                                                                                                                           <  SRR19973080.4550520/150‑1 (MQ=60)
                           CAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTG                                                                                                                          <  SRR19973080.3778718/150‑1 (MQ=60)
                           CAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTG                                                                                                                          >  SRR19973080.1536892/1‑150 (MQ=60)
                            AACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGTGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGC                                                                                                                         <  SRR19973080.3085084/150‑1 (MQ=60)
                            AACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGC                                                                                                                         <  SRR19973080.35527/150‑1 (MQ=60)
                            AACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGC                                                                                                                         <  SRR19973080.3084699/150‑1 (MQ=60)
                            AACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGC                                                                                                                         >  SRR19973080.2601531/1‑150 (MQ=60)
                            AACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGC                                                                                                                         >  SRR19973080.2601590/1‑150 (MQ=60)
                               CGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCG                                                                                                                      >  SRR19973080.1981108/1‑150 (MQ=60)
                               CGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATATCGCCGCTGCGTTGCTCG                                                                                                                      >  SRR19973080.3477126/1‑150 (MQ=60)
                               CGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCG                                                                                                                      >  SRR19973080.1127066/1‑150 (MQ=60)
                               CGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCG                                                                                                                      >  SRR19973080.1126746/1‑150 (MQ=60)
                                   TAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGA                                                                                                                  >  SRR19973080.1308585/1‑150 (MQ=60)
                                   TAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGA                                                                                                                  >  SRR19973080.1763530/1‑150 (MQ=60)
                                    AAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAG                                                                                                                 >  SRR19973080.4185788/1‑150 (MQ=60)
                                      AATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCA                                                                                                               <  SRR19973080.3969404/150‑1 (MQ=60)
                                       ATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAG                                                                                                              <  SRR19973080.303223/150‑1 (MQ=60)
                                       ATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAG                                                                                                              <  SRR19973080.34455/150‑1 (MQ=60)
                                          CTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATT                                                                                                           <  SRR19973080.671706/150‑1 (MQ=60)
                                          CTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATT                                                                                                           <  SRR19973080.671789/150‑1 (MQ=60)
                                           TCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTA                                                                                                          >  SRR19973080.2856124/1‑150 (MQ=60)
                                                AGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTTCTCGCCGAGCAGATTAATGCA                                                                                                     >  SRR19973080.1913328/1‑150 (MQ=60)
                                                AGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCA                                                                                                     <  SRR19973080.1411779/150‑1 (MQ=60)
                                                AGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCA                                                                                                     >  SRR19973080.55625/1‑150 (MQ=60)
                                                AGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCA                                                                                                     <  SRR19973080.289283/150‑1 (MQ=60)
                                                 GCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAG                                                                                                    <  SRR19973080.2974017/150‑1 (MQ=60)
                                                    AAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATG                                                                                                 <  SRR19973080.407007/150‑1 (MQ=60)
                                                        CATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACT                                                                                             >  SRR19973080.2958318/1‑150 (MQ=60)
                                                        CATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACT                                                                                             >  SRR19973080.2958434/1‑150 (MQ=60)
                                                        CATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACT                                                                                             >  SRR19973080.2837016/1‑150 (MQ=60)
                                                         ATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTG                                                                                            <  SRR19973080.899869/150‑1 (MQ=60)
                                                          TCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGG                                                                                           <  SRR19973080.4232504/150‑1 (MQ=60)
                                                            GAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTG                                                                                         >  SRR19973080.3941103/1‑150 (MQ=60)
                                                            GAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTG                                                                                         >  SRR19973080.3941145/1‑150 (MQ=60)
                                                             AGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGA                                                                                        >  SRR19973080.4016406/1‑150 (MQ=60)
                                                             AGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGA                                                                                        >  SRR19973080.4016405/1‑150 (MQ=60)
                                                              GTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGAT                                                                                       <  SRR19973080.3221880/150‑1 (MQ=60)
                                                                 GTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCT                                                                                    >  SRR19973080.2233878/1‑150 (MQ=60)
                                                                 GTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCT                                                                                    >  SRR19973080.3356307/1‑150 (MQ=60)
                                                                  TTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTC                                                                                   <  SRR19973080.4473863/150‑1 (MQ=60)
                                                                    GCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCAC                                                                                 <  SRR19973080.2945983/150‑1 (MQ=60)
                                                                     CTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACC                                                                                <  SRR19973080.147787/150‑1 (MQ=60)
                                                                       CAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCTA                                                                              <  SRR19973080.2072635/150‑1 (MQ=60)
                                                                       CAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGA                                                                              >  SRR19973080.2376507/1‑150 (MQ=60)
                                                                         AAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCCGATTAATGCAGATGGACTCGTGATCCTCACCGATG                                                                            >  SRR19973080.411255/1‑150 (MQ=60)
                                                                            AGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTG                                                                         >  SRR19973080.2289407/1‑150 (MQ=60)
                                                                                  ATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGG                                                                   >  SRR19973080.446600/1‑150 (MQ=60)
                                                                                   TGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGT                                                                  <  SRR19973080.4474770/150‑1 (MQ=60)
                                                                                   TGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGT                                                                  <  SRR19973080.4474795/150‑1 (MQ=60)
                                                                                    GTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTA                                                                 <  SRR19973080.2742311/150‑1 (MQ=60)
                                                                                    GTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTA                                                                 <  SRR19973080.3170261/150‑1 (MQ=60)
                                                                                    GTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTA                                                                 <  SRR19973080.1803541/150‑1 (MQ=60)
                                                                                     TGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTAT                                                                >  SRR19973080.871325/1‑150 (MQ=60)
                                                                                      GGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGTTAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTCTA                                                               >  SRR19973080.210447/1‑150 (MQ=60)
                                                                                        TGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATG                                                             >  SRR19973080.445707/1‑150 (MQ=60)
                                                                                        TGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATG                                                             >  SRR19973080.445783/1‑150 (MQ=60)
                                                                                           TTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAA                                                          <  SRR19973080.3848320/150‑1 (MQ=60)
                                                                                             TGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAAC                                                        >  SRR19973080.3640125/1‑150 (MQ=60)
                                                                                                  TGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGG                                                   >  SRR19973080.361278/1‑150 (MQ=60)
                                                                                                  TGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGG                                                   >  SRR19973080.361217/1‑150 (MQ=60)
                                                                                                      GGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACG                                               <  SRR19973080.910729/150‑1 (MQ=60)
                                                                                                      GGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACG                                               <  SRR19973080.911852/150‑1 (MQ=60)
                                                                                                      GGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACG                                               <  SRR19973080.3191883/150‑1 (MQ=60)
                                                                                                         GGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCG                                            <  SRR19973080.1014696/150‑1 (MQ=60)
                                                                                                         GGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCG                                            >  SRR19973080.3886025/1‑150 (MQ=60)
                                                                                                            GGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAG                                         <  SRR19973080.3149759/150‑1 (MQ=60)
                                                                                                            GGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAG                                         >  SRR19973080.3713959/1‑150 (MQ=60)
                                                                                                            GGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAG                                         <  SRR19973080.869875/150‑1 (MQ=60)
                                                                                                            GGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAG                                         >  SRR19973080.3883098/1‑150 (MQ=60)
                                                                                                            GGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAG                                         >  SRR19973080.3883117/1‑150 (MQ=60)
                                                                                                            GGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAG                                         <  SRR19973080.120842/150‑1 (MQ=60)
                                                                                                              TGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCA                                       <  SRR19973080.1189954/150‑1 (MQ=60)
                                                                                                                  CCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGT                                   >  SRR19973080.2774048/1‑150 (MQ=60)
                                                                                                                   CTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTG                                  <  SRR19973080.1287267/150‑1 (MQ=60)
                                                                                                                      TGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCA                               <  SRR19973080.242826/150‑1 (MQ=60)
                                                                                                                        ACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATT                             <  SRR19973080.412113/150‑1 (MQ=60)
                                                                                                                          GGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCG                           >  SRR19973080.322131/1‑150 (MQ=60)
                                                                                                                             TGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCA                        >  SRR19973080.2716273/1‑150 (MQ=60)
                                                                                                                              GACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCAT                       <  SRR19973080.62117/150‑1 (MQ=60)
                                                                                                                              GACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCAT                       <  SRR19973080.4398262/150‑1 (MQ=60)
                                                                                                                                      AGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACC               <  SRR19973080.2710413/150‑1 (MQ=60)
                                                                                                                                       GGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCG              <  SRR19973080.2922764/150‑1 (MQ=60)
                                                                                                                                        GGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGG             >  SRR19973080.3097105/1‑150 (MQ=60)
                                                                                                                                             GAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAG        >  SRR19973080.1555422/1‑150 (MQ=60)
                                                                                                                                             GAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAG        >  SRR19973080.2052444/1‑150 (MQ=60)
                                                                                                                                              AAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGT       >  SRR19973080.2833845/1‑150 (MQ=60)
                                                                                                                                              AAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGT       <  SRR19973080.4120055/150‑1 (MQ=60)
                                                                                                                                               AGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTT      <  SRR19973080.2023679/150‑1 (MQ=60)
                                                                                                                                               AGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTT      <  SRR19973080.4164367/150‑1 (MQ=60)
                                                                                                                                               AGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTT      <  SRR19973080.1876850/150‑1 (MQ=60)
                                                                                                                                                  AGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGC   >  SRR19973080.2533569/1‑150 (MQ=60)
                                                                                                                                                  AGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGC   >  SRR19973080.3586070/1‑150 (MQ=60)
                                                                                                                                                  AGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGC   >  SRR19973080.52306/1‑150 (MQ=60)
                                                                                                                                                   GTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCG  >  SRR19973080.2665324/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCG  >  CP009273/546342‑546638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑