Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 546,491 G→T 100% V199V (GTG→GTT)  ybcF → putative carbonate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273546,4910GT97.5% 742.1 / 5.4 212V199V (GTG→GTT) ybcFputative carbonate kinase
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (96/109);  total (100/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 219 total aligned reads displayed.

ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGC  >  CP009273/546343‑546637
                                                                                                                                                    |                                                                                                                                                  
  ttGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACTGAGCAGGGAGTGAAGCAGtgatt                                                                                                                                                 <  2:1409997/150‑1 (MQ=255)
  ttGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttatt                                                                                                                                                 >  2:4593155/1‑150 (MQ=255)
   tGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCCGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttattg                                                                                                                                                >  1:5065186/1‑150 (MQ=255)
   tGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGttattg                                                                                                                                                >  1:2604083/1‑150 (MQ=255)
      gcCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATa                                                                                                                                             <  1:1761329/150‑1 (MQ=255)
      gcCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATa                                                                                                                                             <  1:1991893/150‑1 (MQ=255)
         gggTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAg                                                                                                                                          <  1:3296694/150‑1 (MQ=255)
            tggtggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGAtc                                                                                                                                       <  1:2074978/150‑1 (MQ=255)
            tggtggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGAtc                                                                                                                                       >  1:1418040/1‑150 (MQ=255)
              gtggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGAtctc                                                                                                                                     <  1:3241494/150‑1 (MQ=255)
              gtggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACAGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGAtctc                                                                                                                                     <  2:4562504/150‑1 (MQ=255)
               tggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATACAGATCTcg                                                                                                                                    >  1:1443934/1‑150 (MQ=255)
               tggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcg                                                                                                                                    <  2:3785892/150‑1 (MQ=255)
               tggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcg                                                                                                                                    >  1:755128/1‑150 (MQ=255)
                ggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgc                                                                                                                                   <  1:287071/150‑1 (MQ=255)
                ggCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTcgc                                                                                                                                   >  1:2460681/1‑150 (MQ=255)
                    tctcCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCt                                                                                                                               >  1:4352690/1‑150 (MQ=255)
                      tcCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGc                                                                                                                             <  2:2670772/150‑1 (MQ=255)
                       ccGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCg                                                                                                                            >  2:1087482/1‑150 (MQ=255)
                         gCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGtt                                                                                                                          <  2:3109983/150‑1 (MQ=255)
                         gCAACCGCGTAAAATTCTCGACAGCGAAGCCATAGAGTTGTTGCTCAATGAGGGGCTTGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCTGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGtt                                                                                                                          <  1:4989933/150‑1 (MQ=255)
                             ccGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTc                                                                                                                      <  1:738034/150‑1 (MQ=255)
                             ccGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTc                                                                                                                      <  1:1699167/150‑1 (MQ=255)
                             ccGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTc                                                                                                                      <  1:4642835/150‑1 (MQ=255)
                              cgcgTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCg                                                                                                                     >  1:2399606/1‑150 (MQ=255)
                                  tAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGa                                                                                                                 >  1:3114478/1‑150 (MQ=255)
                                  tAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGa                                                                                                                 <  2:4710338/150‑1 (MQ=255)
                                       ttCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGa                                                                                                            >  1:675724/1‑150 (MQ=255)
                                       ttCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGa                                                                                                            >  2:741688/1‑150 (MQ=255)
                                         ctcGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGAtt                                                                                                          <  1:1731815/150‑1 (MQ=255)
                                           cGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTaa                                                                                                        <  2:303530/150‑1 (MQ=255)
                                            gACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAAt                                                                                                       >  1:101097/1‑150 (MQ=255)
                                            gACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAAt                                                                                                       <  2:4817394/150‑1 (MQ=255)
                                            gACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAAt                                                                                                       >  2:333357/1‑150 (MQ=255)
                                              cAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGc                                                                                                     >  2:1003423/1‑150 (MQ=255)
                                               aGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCa                                                                                                    <  2:1490379/150‑1 (MQ=255)
                                               aGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCa                                                                                                    >  1:5127575/1‑150 (MQ=255)
                                                gCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAg                                                                                                   <  2:4394925/150‑1 (MQ=255)
                                                  gAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGAt                                                                                                 <  1:4552095/150‑1 (MQ=255)
                                                  gAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGAt                                                                                                 >  2:1526361/1‑150 (MQ=255)
                                                  gAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGAt                                                                                                 <  2:2232521/150‑1 (MQ=255)
                                                     gCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGa                                                                                              <  1:3505151/150‑1 (MQ=255)
                                                      ccATCGTGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGAc                                                                                             <  2:1745654/150‑1 (MQ=255)
                                                      ccATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGAc                                                                                             >  2:1569423/1‑150 (MQ=255)
                                                      ccATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGAc                                                                                             >  1:2659409/1‑150 (MQ=255)
                                                      ccATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGAc                                                                                             >  1:4167873/1‑150 (MQ=255)
                                                        aTCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTg                                                                                           >  2:2974790/1‑150 (MQ=255)
                                                          cGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGt                                                                                         <  2:2775351/150‑1 (MQ=255)
                                                            aGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGa                                                                                       <  1:1097666/150‑1 (MQ=255)
                                                             gttgttGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGAt                                                                                      >  2:4221528/1‑150 (MQ=255)
                                                             gttgttGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGAt                                                                                      <  1:4907012/150‑1 (MQ=255)
                                                             gttgttGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGAt                                                                                      >  2:1627590/1‑150 (MQ=255)
                                                             gttgttGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGAt                                                                                      >  2:4997269/1‑150 (MQ=255)
                                                               tgttgCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATcc                                                                                    <  2:5078244/150‑1 (MQ=255)
                                                                  tgCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCa                                                                                 >  1:409368/1‑150 (MQ=255)
                                                                     tCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCg                                                                              >  2:2832574/1‑150 (MQ=255)
                                                                     tCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCg                                                                              <  1:39647/150‑1 (MQ=255)
                                                                       aaaGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGAt                                                                            <  2:1107522/150‑1 (MQ=255)
                                                                       aaaGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGAt                                                                            <  2:4100180/150‑1 (MQ=255)
                                                                         agagGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGc                                                                          <  2:4551840/150‑1 (MQ=255)
                                                                            ggggCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGa                                                                       <  1:2341601/150‑1 (MQ=255)
                                                                             gggCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGGTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGAt                                                                      <  1:4586263/150‑1 (MQ=255)
                                                                                 aTGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCgg                                                                  <  1:3862688/150‑1 (MQ=255)
                                                                                  tgtgGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGt                                                                 <  2:393939/150‑1 (MQ=255)
                                                                                  tgtgGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGt                                                                 <  2:3673865/150‑1 (MQ=255)
                                                                                  tgtgGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGt                                                                 <  2:3383626/150‑1 (MQ=255)
                                                                                   gtggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGta                                                                >  1:3713012/1‑150 (MQ=255)
                                                                                    tggtgATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGtat                                                               >  2:3446262/1‑150 (MQ=255)
                                                                                          tttGCAGTGGCGGCGGTGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGaaa                                                         >  2:258681/1‑150 (MQ=255)
                                                                                            tGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAAc                                                       <  2:2009723/150‑1 (MQ=255)
                                                                                             gCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACt                                                      <  1:2111898/150‑1 (MQ=255)
                                                                                              cAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTg                                                     <  2:1595605/150‑1 (MQ=255)
                                                                                               aGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgg                                                    >  2:1822789/1‑150 (MQ=255)
                                                                                               aGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTgg                                                    >  1:1177212/1‑150 (MQ=255)
                                                                                                  ggcggcggcGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGa                                                 <  1:3388722/150‑1 (MQ=255)
                                                                                                  ggcggcggcGGTGGGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGGTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGa                                                 <  2:4096997/150‑1 (MQ=255)
                                                                                                     ggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcg                                              >  2:5083607/1‑150 (MQ=255)
                                                                                                     ggcggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcg                                              >  2:3914424/1‑150 (MQ=255)
                                                                                                       cggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcgcc                                            <  2:1759230/150‑1 (MQ=255)
                                                                                                        ggcggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAAcgccg                                           <  2:80720/150‑1 (MQ=255)
                                                                                                           ggTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTCATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCgcag                                        >  2:1790224/1‑150 (MQ=255)
                                                                                                             tgtgCCTGTGACGGATGACGGAGCCGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCTCCGATGCTGATGCGGGATAGGAAAACTGGGGAACGCCgcagca                                      >  2:966476/1‑150 (MQ=255)
                                                                                                               tgCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAAc                                    >  2:2054834/1‑150 (MQ=255)
                                                                                                                 ccTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTACTGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGt                                  <  1:459684/150‑1 (MQ=255)
                                                                                                                  cTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTg                                 <  2:1327170/150‑1 (MQ=255)
                                                                                                                  cTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTg                                 >  2:59464/1‑150 (MQ=255)
                                                                                                                  cTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTg                                 >  2:4695729/1‑150 (MQ=255)
                                                                                                                         ggatgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCg                          >  1:3426593/1‑150 (MQ=255)
                                                                                                                           atgacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGcc                        >  1:355935/1‑150 (MQ=255)
                                                                                                                             gacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCAt                      <  2:793736/150‑1 (MQ=255)
                                                                                                                             gacggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCAt                      >  2:1522033/1‑150 (MQ=255)
                                                                                                                               cggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGc                    >  1:238830/1‑150 (MQ=255)
                                                                                                                                ggaGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGcc                   >  2:859940/1‑150 (MQ=255)
                                                                                                                                   gCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCcaca                >  2:2322913/1‑150 (MQ=255)
                                                                                                                                   gCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCcaca                >  1:3926371/1‑150 (MQ=255)
                                                                                                                                      gggAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCg             >  2:218072/1‑150 (MQ=255)
                                                                                                                                           tGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGa        <  1:1540959/150‑1 (MQ=255)
                                                                                                                                           tGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGa        <  2:2720682/150‑1 (MQ=255)
                                                                                                                                             aaGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGt      <  2:2548114/150‑1 (MQ=255)
                                                                                                                                                 aGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAgc  >  1:582674/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGC  >  CP009273/546343‑546637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGC  >  CP009273/546343‑546639
                                                                                                                                                    |                                                                                                                                                    
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     <  SRR19973083.4172697/150‑1 (MQ=60)
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     >  SRR19973083.2204515/1‑150 (MQ=60)
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     >  SRR19973083.638362/1‑150 (MQ=60)
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     >  SRR19973083.2606790/1‑150 (MQ=60)
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     >  SRR19973083.2606869/1‑150 (MQ=60)
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     >  SRR19973083.638329/1‑150 (MQ=60)
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     >  SRR19973083.5195233/1‑150 (MQ=60)
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     >  SRR19973083.1326484/1‑150 (MQ=60)
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     >  SRR19973083.3935086/1‑150 (MQ=60)
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     >  SRR19973083.3934991/1‑150 (MQ=60)
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     >  SRR19973083.1120899/1‑150 (MQ=60)
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTA                                                                                                                                                     >  SRR19973083.1120872/1‑150 (MQ=60)
 TTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTAT                                                                                                                                                    <  SRR19973083.4592939/150‑1 (MQ=60)
   TGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCCGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTG                                                                                                                                                  >  SRR19973083.5086865/1‑150 (MQ=60)
   TGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTG                                                                                                                                                  >  SRR19973083.2617188/1‑150 (MQ=60)
      GCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATA                                                                                                                                               <  SRR19973083.1770018/150‑1 (MQ=60)
      GCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATA                                                                                                                                               <  SRR19973083.2002070/150‑1 (MQ=60)
       CCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGAAAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTTTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAA                                                                                                                                              <  SRR19973083.2409388/150‑1 (MQ=60)
         GGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAG                                                                                                                                            <  SRR19973083.3543905/150‑1 (MQ=60)
         GGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAG                                                                                                                                            <  SRR19973083.3312196/150‑1 (MQ=60)
            TGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATC                                                                                                                                         >  SRR19973083.1425136/1‑150 (MQ=60)
            TGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATC                                                                                                                                         <  SRR19973083.2085949/150‑1 (MQ=60)
            TGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATC                                                                                                                                         <  SRR19973083.2085526/150‑1 (MQ=60)
            TGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATC                                                                                                                                         >  SRR19973083.1425273/1‑150 (MQ=60)
              GTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTC                                                                                                                                       <  SRR19973083.3256829/150‑1 (MQ=60)
              GTGGCGTCTCCGCAACCGCGTAAAATTCTAGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGTGAGTGAAGCAGTTATTGATAAAGATCTC                                                                                                                                       <  SRR19973083.3256789/150‑1 (MQ=60)
               TGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATACAGATCTCG                                                                                                                                      >  SRR19973083.1451194/1‑150 (MQ=60)
               TGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCG                                                                                                                                      >  SRR19973083.758822/1‑150 (MQ=60)
                GGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGC                                                                                                                                     <  SRR19973083.288561/150‑1 (MQ=60)
                GGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGC                                                                                                                                     >  SRR19973083.2473280/1‑150 (MQ=60)
                   GTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGC                                                                                                                                  >  SRR19973083.923228/1‑150 (MQ=60)
                    TCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCT                                                                                                                                 >  SRR19973083.4371574/1‑150 (MQ=60)
                    TCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCT                                                                                                                                 <  SRR19973083.743802/150‑1 (MQ=60)
                    TCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCT                                                                                                                                 <  SRR19973083.3360660/150‑1 (MQ=60)
                     CTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTG                                                                                                                                >  SRR19973083.4757713/1‑150 (MQ=60)
                     CTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTG                                                                                                                                >  SRR19973083.4757757/1‑150 (MQ=60)
                      TCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGCGTGAAGCAGGTATTGATAAAGATCTCGCCGCTGC                                                                                                                               >  SRR19973083.2641796/1‑150 (MQ=60)
                         GCAACCGCGTAAAATTCTCGACAGCGAAGCCATAGAGTTGTTGCTCAATGAGGGGCTTGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCTGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTT                                                                                                                            <  SRR19973083.5011362/150‑1 (MQ=60)
                          CAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTG                                                                                                                           <  SRR19973083.1120435/150‑1 (MQ=60)
                            ACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCT                                                                                                                         >  SRR19973083.3877041/1‑150 (MQ=60)
                            ACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCT                                                                                                                         >  SRR19973083.5182723/1‑150 (MQ=60)
                             CCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTC                                                                                                                        <  SRR19973083.4662842/150‑1 (MQ=60)
                             CCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTC                                                                                                                        <  SRR19973083.741665/150‑1 (MQ=60)
                             CCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTC                                                                                                                        <  SRR19973083.1707540/150‑1 (MQ=60)
                              CGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCG                                                                                                                       >  SRR19973083.2411926/1‑150 (MQ=60)
                                  TAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGA                                                                                                                   <  SRR19973083.2238887/150‑1 (MQ=60)
                                  TAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCAGCGATGCTCGCCGA                                                                                                                   >  SRR19973083.3129391/1‑150 (MQ=60)
                                       TTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGA                                                                                                              >  SRR19973083.679108/1‑150 (MQ=60)
                                       TTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGA                                                                                                              <  SRR19973083.643870/150‑1 (MQ=60)
                                       TTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGA                                                                                                              >  SRR19973083.679103/1‑150 (MQ=60)
                                         CTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATT                                                                                                            <  SRR19973083.1740304/150‑1 (MQ=60)
                                            GACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAAT                                                                                                         >  SRR19973083.101770/1‑150 (MQ=60)
                                            GACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAAT                                                                                                         <  SRR19973083.1728183/150‑1 (MQ=60)
                                             ACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATG                                                                                                        <  SRR19973083.125722/150‑1 (MQ=60)
                                               AGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCA                                                                                                      >  SRR19973083.5149399/1‑150 (MQ=60)
                                                 CGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGA                                                                                                    <  SRR19973083.335632/150‑1 (MQ=60)
                                                  GAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGAT                                                                                                   >  SRR19973083.2713567/1‑150 (MQ=60)
                                                  GAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGAT                                                                                                   <  SRR19973083.4571822/150‑1 (MQ=60)
                                                   AAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATG                                                                                                  <  SRR19973083.2355147/150‑1 (MQ=60)
                                                   AAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATG                                                                                                  <  SRR19973083.690241/150‑1 (MQ=60)
                                                    AGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGG                                                                                                 >  SRR19973083.1518092/1‑150 (MQ=60)
                                                     GCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGA                                                                                                <  SRR19973083.3521210/150‑1 (MQ=60)
                                                     GCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGA                                                                                                <  SRR19973083.3521221/150‑1 (MQ=60)
                                                      CCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGAC                                                                                               >  SRR19973083.2672756/1‑150 (MQ=60)
                                                      CCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGAC                                                                                               >  SRR19973083.4186249/1‑150 (MQ=60)
                                                      CCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGAC                                                                                               <  SRR19973083.2107048/150‑1 (MQ=60)
                                                       CATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACT                                                                                              <  SRR19973083.2427934/150‑1 (MQ=60)
                                                        ATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTG                                                                                             >  SRR19973083.2552769/1‑150 (MQ=60)
                                                         TCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGG                                                                                            <  SRR19973083.1597832/150‑1 (MQ=60)
                                                           GAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTG                                                                                          >  SRR19973083.1963123/1‑150 (MQ=60)
                                                            AGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGA                                                                                         <  SRR19973083.1103194/150‑1 (MQ=60)
                                                            AGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGA                                                                                         <  SRR19973083.5088602/150‑1 (MQ=60)
                                                             GTTGTTGCTCAAAGAGGGGGATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGAT                                                                                        <  SRR19973083.1021243/150‑1 (MQ=60)
                                                             GTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGAT                                                                                        <  SRR19973083.4928111/150‑1 (MQ=60)
                                                                GTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGTCGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCT                                                                                     <  SRR19973083.2579314/150‑1 (MQ=60)
                                                                  TGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCA                                                                                   >  SRR19973083.411213/1‑150 (MQ=60)
                                                                  TGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCA                                                                                   >  SRR19973083.4639214/1‑150 (MQ=60)
                                                                   GCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCAC                                                                                  <  SRR19973083.4946730/150‑1 (MQ=60)
                                                                     TCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCG                                                                                <  SRR19973083.39963/150‑1 (MQ=60)
                                                                            GGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGA                                                                         <  SRR19973083.2353642/150‑1 (MQ=60)
                                                                             GGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGGTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGAT                                                                        <  SRR19973083.4606083/150‑1 (MQ=60)
                                                                              GGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATG                                                                       <  SRR19973083.1761772/150‑1 (MQ=60)
                                                                                 ATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGG                                                                    <  SRR19973083.3880780/150‑1 (MQ=60)
                                                                                 ATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGG                                                                    <  SRR19973083.3879971/150‑1 (MQ=60)
                                                                                   GTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTA                                                                  >  SRR19973083.3729906/1‑150 (MQ=60)
                                                                                             GCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACT                                                        <  SRR19973083.2122571/150‑1 (MQ=60)
                                                                                               AGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGG                                                      >  SRR19973083.1183061/1‑150 (MQ=60)
                                                                                                  GGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGA                                                   <  SRR19973083.3404419/150‑1 (MQ=60)
                                                                                                  GGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGA                                                   <  SRR19973083.3405152/150‑1 (MQ=60)
                                                                                                         GCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGC                                            <  SRR19973083.4252383/150‑1 (MQ=60)
                                                                                                         GCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGC                                            <  SRR19973083.4252312/150‑1 (MQ=60)
                                                                                                              GTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAA                                       >  SRR19973083.1594641/1‑150 (MQ=60)
                                                                                                                 CCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTACTGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGT                                    <  SRR19973083.461807/150‑1 (MQ=60)
                                                                                                                   TGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGC                                  >  SRR19973083.2936401/1‑150 (MQ=60)
                                                                                                                   TGTGACGGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGC                                  >  SRR19973083.5037858/1‑150 (MQ=60)
                                                                                                                         GGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCG                            <  SRR19973083.3974554/150‑1 (MQ=60)
                                                                                                                         GGATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCG                            >  SRR19973083.3442365/1‑150 (MQ=60)
                                                                                                                           ATGACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCC                          >  SRR19973083.357621/1‑150 (MQ=60)
                                                                                                                             GACGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCAT                        <  SRR19973083.3304657/150‑1 (MQ=60)
                                                                                                                               CGGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGC                      >  SRR19973083.240118/1‑150 (MQ=60)
                                                                                                                                GGAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCC                     <  SRR19973083.4029847/150‑1 (MQ=60)
                                                                                                                                 GAGCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCA                    <  SRR19973083.3655201/150‑1 (MQ=60)
                                                                                                                                   GCAGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACA                  >  SRR19973083.3943851/1‑150 (MQ=60)
                                                                                                                                     AGGGAGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACC                <  SRR19973083.2047798/150‑1 (MQ=60)
                                                                                                                                         AGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGAT            <  SRR19973083.4612991/150‑1 (MQ=60)
                                                                                                                                         AGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGAT            >  SRR19973083.4133614/1‑150 (MQ=60)
                                                                                                                                         AGTGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGAT            <  SRR19973083.3852406/150‑1 (MQ=60)
                                                                                                                                           TGAAGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGA          <  SRR19973083.1548712/150‑1 (MQ=60)
                                                                                                                                              AGCAGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTT       <  SRR19973083.4382396/150‑1 (MQ=60)
                                                                                                                                                 AGTTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGC    >  SRR19973083.585600/1‑150 (MQ=60)
                                                                                                                                                   TTATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGC  >  SRR19973083.3968805/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
ATTTGCGCCGGGTGGTGGCGTCTCCGCAACCGCGTAAAATTCTCGACAGCGAAGCCATCGAGTTGTTGCTCAAAGAGGGGCATGTGGTGATTTGCAGTGGCGGCGGCGGTGTGCCTGTGACGGATGACGGAGCAGGGAGTGAAGCAGTGATTGATAAAGATCTCGCCGCTGCGTTGCTCGCCGAGCAGATTAATGCAGATGGACTGGTGATCCTCACCGATGCTGATGCGGTATATGAAAACTGGGGAACGCCGCAGCAACGTGCCATTCGCCATGCCACACCGGATGAGTTAGCGC  >  CP009273/546343‑546639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑