Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 583,341 T→G 93.8% S33R (AGT→CGT)  ybcH ← PRK09936 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273583,3410TG93.8% 1050.9 / 31.4 275S33R (AGT→CGT) ybcHPRK09936 family protein
Reads supporting (aligned to +/- strand):  ref base T (7/10);  new base G (124/134);  total (131/144)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 276 total aligned reads displayed.

CAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACGTT  >  CP009273/583194‑583490
                                                                                                                                                   |                                                                                                                                                     
cAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGAt                                                                                                                                                     <  1:2880591/150‑1 (MQ=255)
 aGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGAtc                                                                                                                                                    >  2:3313258/1‑150 (MQ=255)
 aGCTGCGGCCCGCTTAAACAAGAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGAtc                                                                                                                                                    <  2:4211154/150‑1 (MQ=255)
   cTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGAtctc                                                                                                                                                  <  1:923229/150‑1 (MQ=255)
    tGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCg                                                                                                                                                 <  1:3136800/150‑1 (MQ=255)
      cGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGt                                                                                                                                               >  2:4250227/1‑150 (MQ=255)
      cGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCAACAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGt                                                                                                                                               >  1:2668417/1‑150 (MQ=255)
       ggCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGtt                                                                                                                                              <  1:2161033/150‑1 (MQ=255)
         cccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGtttt                                                                                                                                            >  2:3242193/1‑150 (MQ=255)
         cccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGtttt                                                                                                                                            >  2:4052167/1‑150 (MQ=255)
          ccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtg                                                                                                                                           >  1:1769222/1‑150 (MQ=255)
          ccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtg                                                                                                                                           >  1:3850775/1‑150 (MQ=255)
            gCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtgtg                                                                                                                                         >  2:252963/1‑150 (MQ=255)
             cTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTgg                                                                                                                                        <  1:1599989/150‑1 (MQ=255)
               tAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGtt                                                                                                                                      <  2:2639983/150‑1 (MQ=255)
                aaaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTg                                                                                                                                     >  1:2836065/1‑150 (MQ=255)
                aaaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTg                                                                                                                                     <  2:821367/150‑1 (MQ=255)
                   cAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCa                                                                                                                                  <  1:2052742/150‑1 (MQ=255)
                    aaTAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAg                                                                                                                                 <  2:1771486/150‑1 (MQ=255)
                         cGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGataat                                                                                                                            <  2:57949/150‑1 (MQ=255)
                              gcTGTTCTGGCTGGGTAACTGCATCGCCGTAACGGGTCCATTGCAAAACAAGGTTATCGAAGCCTTTCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCtt                                                                                                                       <  1:1587477/150‑1 (MQ=255)
                                tGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTc                                                                                                                     <  1:4365743/150‑1 (MQ=255)
                                tGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTc                                                                                                                     <  2:477867/150‑1 (MQ=255)
                                 gTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCa                                                                                                                    <  1:1757159/150‑1 (MQ=255)
                                 attCTGGCTGGGTAAATGCATCGCCGTAACGGGTCAATTGAAAAACAAGGGTATCGAAGACTTGCAAACGTAACTGACTCATCAGCACCTGAAACTGGGTATCGGTAAACTGACGAACTATGTTATGTGGTTGACAGATAAAACCtataa                                                                                                                    >  2:3455873/2‑146 (MQ=255)
                                  ttCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCAt                                                                                                                   <  2:3147964/150‑1 (MQ=255)
                                          gggTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAgg                                                                                                           <  1:1461530/150‑1 (MQ=255)
                                           ggTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGa                                                                                                          <  2:2422589/150‑1 (MQ=255)
                                            gTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaa                                                                                                         <  2:1093336/150‑1 (MQ=255)
                                             tAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaa                                                                                                        >  1:208114/1‑150 (MQ=255)
                                             tAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaa                                                                                                        <  2:2947375/150‑1 (MQ=255)
                                                 tGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAgg                                                                                                    >  2:1409975/1‑150 (MQ=255)
                                                 tGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAgg                                                                                                    >  2:3911909/1‑150 (MQ=255)
                                                  gCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAggg                                                                                                   <  1:1817661/150‑1 (MQ=255)
                                                   cATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAATGGAAAAAGGGc                                                                                                  >  1:1872497/1‑150 (MQ=255)
                                                    aTCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCt                                                                                                 <  1:4346403/150‑1 (MQ=255)
                                                      cGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGa                                                                                               <  2:1271602/150‑1 (MQ=255)
                                                      cGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGa                                                                                               <  2:2667420/150‑1 (MQ=255)
                                                         cGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCa                                                                                            <  2:2976277/150‑1 (MQ=255)
                                                           tAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCaaa                                                                                          >  1:2367015/1‑150 (MQ=255)
                                                           tAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCaaa                                                                                          >  2:2182260/1‑150 (MQ=255)
                                                           tAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCaaa                                                                                          >  1:734672/1‑150 (MQ=255)
                                                               gggTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCa                                                                                      >  2:2566830/1‑150 (MQ=255)
                                                                 gTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgt                                                                                    <  2:130868/150‑1 (MQ=255)
                                                                 gTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgt                                                                                    <  1:3938544/150‑1 (MQ=255)
                                                                 gTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgt                                                                                    <  2:4461372/150‑1 (MQ=255)
                                                                    cATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTc                                                                                 <  1:899930/150‑1 (MQ=255)
                                                                     aTTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCa                                                                                >  1:1257394/1‑150 (MQ=255)
                                                                     aTTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCa                                                                                >  1:377848/1‑150 (MQ=255)
                                                                        gCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCa                                                                             >  1:177920/1‑150 (MQ=255)
                                                                        gCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCa                                                                             <  2:3110611/150‑1 (MQ=255)
                                                                        gCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCa                                                                             <  2:2252873/150‑1 (MQ=255)
                                                                         cAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCaa                                                                            >  2:2105873/1‑150 (MQ=255)
                                                                          aaaaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAAt                                                                           <  2:88400/150‑1 (MQ=255)
                                                                           aaaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATa                                                                          <  1:2302262/150‑1 (MQ=255)
                                                                            aaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAc                                                                         >  2:2176934/1‑150 (MQ=255)
                                                                               aaGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaa                                                                      >  2:3546562/1‑150 (MQ=255)
                                                                                aGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaaa                                                                     <  1:1010280/150‑1 (MQ=255)
                                                                                   gTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATg                                                                  <  2:1610155/150‑1 (MQ=255)
                                                                                    tATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGa                                                                 >  2:1956236/1‑150 (MQ=255)
                                                                                      tCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAAc                                                               <  1:4537149/150‑1 (MQ=255)
                                                                                      tCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGGTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAACAGCAGTGTCAGCAATACGAAAATGAAc                                                               <  1:2261323/150‑1 (MQ=255)
                                                                                       cGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAATGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACt                                                              >  1:3090380/1‑150 (MQ=255)
                                                                                        gAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACtt                                                             >  1:3011458/1‑150 (MQ=255)
                                                                                         aaGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTa                                                            >  2:2474245/1‑150 (MQ=255)
                                                                                          aGCCTTGCTAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTAc                                                           <  1:1324874/150‑1 (MQ=255)
                                                                                          aGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTAc                                                           <  1:584573/150‑1 (MQ=255)
                                                                                          aGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTAc                                                           <  2:2497806/150‑1 (MQ=255)
                                                                                            ccTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGc                                                         >  2:4433377/1‑150 (MQ=255)
                                                                                              ttGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAt                                                       <  1:2458487/150‑1 (MQ=255)
                                                                                               tGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAtt                                                      <  2:2769658/150‑1 (MQ=255)
                                                                                                gCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAttt                                                     >  1:4509765/1‑150 (MQ=255)
                                                                                                 cAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTa                                                    >  2:1052231/1‑150 (MQ=255)
                                                                                                    aCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCa                                                 >  1:4456906/1‑150 (MQ=255)
                                                                                                      gTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGt                                               >  1:2410728/1‑150 (MQ=255)
                                                                                                        aaCTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGc                                             >  1:2580034/1‑150 (MQ=255)
                                                                                                        aaCTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGc                                             <  2:3645310/150‑1 (MQ=255)
                                                                                                        aaCTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGc                                             >  1:3233676/1‑150 (MQ=255)
                                                                                                         aCTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCa                                            >  2:2226082/1‑150 (MQ=255)
                                                                                                             aCTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTcc                                        >  2:1663421/1‑150 (MQ=255)
                                                                                                             aCTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTcc                                        >  2:3349587/1‑150 (MQ=255)
                                                                                                             aCTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTcc                                        >  2:3871877/1‑150 (MQ=255)
                                                                                                                catcaGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCact                                     >  2:4163308/1‑148 (MQ=255)
                                                                                                                    aGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTg                                 >  2:2364921/1‑150 (MQ=255)
                                                                                                                        ccTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGaa                             <  2:3606305/150‑1 (MQ=255)
                                                                                                                        ccTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGaa                             <  2:648178/150‑1 (MQ=255)
                                                                                                                          tGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAc                           <  2:3850969/150‑1 (MQ=255)
                                                                                                                          tGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAc                           >  1:3660459/1‑150 (MQ=255)
                                                                                                                             cACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAcgcg                        >  1:3437385/1‑150 (MQ=255)
                                                                                                                               cTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgtt                      <  2:340741/150‑1 (MQ=255)
                                                                                                                                 gggTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgttgt                    >  2:1552663/1‑150 (MQ=255)
                                                                                                                                 gggTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgttgt                    <  1:3463713/150‑1 (MQ=255)
                                                                                                                                  ggTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgttgtt                   <  1:3966231/150‑1 (MQ=255)
                                                                                                                                  ggTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgttgtt                   <  1:2979857/150‑1 (MQ=255)
                                                                                                                                     aTCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTgcg                <  2:2730063/150‑1 (MQ=255)
                                                                                                                                             ccTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGt        >  2:3842306/1‑150 (MQ=255)
                                                                                                                                              cTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGtt       >  1:2543025/1‑150 (MQ=255)
                                                                                                                                              cTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGtt       <  2:3731229/150‑1 (MQ=255)
                                                                                                                                               tGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTa      <  1:4052167/150‑1 (MQ=255)
                                                                                                                                                   tATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACGtt  <  2:1646377/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACGTT  >  CP009273/583194‑583490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTAC  >  CP009273/583192‑583487
                                                                                                                                                     |                                                                                                                                                  
CGCAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACG                                                                                                                                                    <  SRR19973080.2122401/150‑1 (MQ=60)
  CAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTAT                                                                                                                                                  >  SRR19973080.2311024/1‑150 (MQ=60)
  CAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGAT                                                                                                                                                  <  SRR19973080.2896353/150‑1 (MQ=60)
     CTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTC                                                                                                                                               <  SRR19973080.928885/150‑1 (MQ=60)
     CTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTC                                                                                                                                               <  SRR19973080.2318857/150‑1 (MQ=60)
      TGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCG                                                                                                                                              <  SRR19973080.3154066/150‑1 (MQ=60)
       GCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGG                                                                                                                                             <  SRR19973080.1618889/150‑1 (MQ=60)
        CGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCAACAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGT                                                                                                                                            >  SRR19973080.2682921/1‑150 (MQ=60)
         GGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTT                                                                                                                                           <  SRR19973080.2173112/150‑1 (MQ=60)
          GCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTT                                                                                                                                          <  SRR19973080.2893638/150‑1 (MQ=60)
          GCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTT                                                                                                                                          <  SRR19973080.2893605/150‑1 (MQ=60)
          GCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTT                                                                                                                                          >  SRR19973080.817544/1‑150 (MQ=60)
            CCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTG                                                                                                                                        >  SRR19973080.3871938/1‑150 (MQ=60)
            CCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTG                                                                                                                                        >  SRR19973080.3871949/1‑150 (MQ=60)
            CCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTG                                                                                                                                        >  SRR19973080.3872145/1‑150 (MQ=60)
            CCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTG                                                                                                                                        <  SRR19973080.2262342/150‑1 (MQ=60)
            CCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTG                                                                                                                                        >  SRR19973080.1779373/1‑150 (MQ=60)
            CCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTG                                                                                                                                        >  SRR19973080.1779379/1‑150 (MQ=60)
               CTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGG                                                                                                                                     <  SRR19973080.1609346/150‑1 (MQ=60)
                TTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTTCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGT                                                                                                                                    <  SRR19973080.996892/150‑1 (MQ=60)
                TTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGT                                                                                                                                    >  SRR19973080.2797972/1‑150 (MQ=60)
                TTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGT                                                                                                                                    <  SRR19973080.1463393/150‑1 (MQ=60)
                  AAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTG                                                                                                                                  >  SRR19973080.2851649/1‑150 (MQ=60)
                  AAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTG                                                                                                                                  <  SRR19973080.2939970/150‑1 (MQ=60)
                     CAATCACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCA                                                                                                                               <  SRR19973080.2064421/150‑1 (MQ=60)
                     CAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCA                                                                                                                               >  SRR19973080.173265/1‑150 (MQ=60)
                     CAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCA                                                                                                                               <  SRR19973080.2064394/150‑1 (MQ=60)
                      AATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAG                                                                                                                              <  SRR19973080.3727048/150‑1 (MQ=60)
                        TAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACCAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGAT                                                                                                                            <  SRR19973080.910224/150‑1 (MQ=60)
                        TAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGAT                                                                                                                            <  SRR19973080.910985/150‑1 (MQ=60)
                         AACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATA                                                                                                                           <  SRR19973080.4517103/150‑1 (MQ=60)
                         AACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATA                                                                                                                           <  SRR19973080.4517048/150‑1 (MQ=60)
                            GTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATA                                                                                                                        <  SRR19973080.2098366/150‑1 (MQ=60)
                             TGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATAC                                                                                                                       <  SRR19973080.2980017/150‑1 (MQ=60)
                               CGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCT                                                                                                                     <  SRR19973080.4289625/150‑1 (MQ=60)
                                GCTGTTCTGGCTGGGTAACTGCATCGCCGTAACGGGTCCATTGCAAAACAAGGTTATCGAAGCCTTTCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTT                                                                                                                    <  SRR19973080.1596765/150‑1 (MQ=60)
                                GCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTT                                                                                                                    <  SRR19973080.1596788/150‑1 (MQ=60)
                                  TGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTC                                                                                                                  <  SRR19973080.4389555/150‑1 (MQ=60)
                                   GTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCA                                                                                                                 <  SRR19973080.1767283/150‑1 (MQ=60)
                                      CTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCG                                                                                                              <  SRR19973080.4027224/150‑1 (MQ=60)
                                            GGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGG                                                                                                        <  SRR19973080.1469972/150‑1 (MQ=60)
                                             GGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGA                                                                                                       >  SRR19973080.3560110/1‑150 (MQ=60)
                                               TAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAA                                                                                                     >  SRR19973080.209654/1‑150 (MQ=60)
                                                    GCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGG                                                                                                <  SRR19973080.1827946/150‑1 (MQ=60)
                                                     CATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAATGGAAAAAGGGC                                                                                               >  SRR19973080.1882947/1‑150 (MQ=60)
                                                     CATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGC                                                                                               >  SRR19973080.2224967/1‑150 (MQ=60)
                                                     CATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGC                                                                                               <  SRR19973080.4543291/150‑1 (MQ=60)
                                                     CATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGC                                                                                               >  SRR19973080.790031/1‑150 (MQ=60)
                                                     CATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGC                                                                                               >  SRR19973080.1882784/1‑150 (MQ=60)
                                                      ATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAATCGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCT                                                                                              <  SRR19973080.2082518/150‑1 (MQ=60)
                                                      ATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCT                                                                                              <  SRR19973080.4371906/150‑1 (MQ=60)
                                                      ATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCT                                                                                              <  SRR19973080.4370131/150‑1 (MQ=60)
                                                        CGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGA                                                                                            <  SRR19973080.3267423/150‑1 (MQ=60)
                                                           CGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCA                                                                                         >  SRR19973080.3091760/1‑150 (MQ=60)
                                                             TAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGACAAAGGGCTGACCAAA                                                                                       >  SRR19973080.2379948/1‑150 (MQ=60)
                                                             TAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAA                                                                                       >  SRR19973080.2380186/1‑150 (MQ=60)
                                                             TAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAA                                                                                       >  SRR19973080.738868/1‑150 (MQ=60)
                                                                CGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGC                                                                                    >  SRR19973080.384605/1‑150 (MQ=60)
                                                                 GGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGGTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCA                                                                                   <  SRR19973080.239043/150‑1 (MQ=60)
                                                                   GTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGT                                                                                 <  SRR19973080.3960264/150‑1 (MQ=60)
                                                                      CATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTC                                                                              >  SRR19973080.2011664/1‑150 (MQ=60)
                                                                      CATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTC                                                                              >  SRR19973080.2011420/1‑150 (MQ=60)
                                                                      CATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCGAGATAATACCTTTCAGCGCAAAGGAAAAAGGGCTGACCAACAGCAGTGTC                                                                              >  SRR19973080.3112992/1‑150 (MQ=60)
                                                                      CATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTC                                                                              <  SRR19973080.905441/150‑1 (MQ=60)
                                                                       ATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCA                                                                             >  SRR19973080.379985/1‑150 (MQ=60)
                                                                       ATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCA                                                                             >  SRR19973080.1265017/1‑150 (MQ=60)
                                                                          GCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCACAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCA                                                                          >  SRR19973080.179277/1‑150 (MQ=60)
                                                                          GCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCA                                                                          >  SRR19973080.179292/1‑150 (MQ=60)
                                                                          GCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCA                                                                          >  SRR19973080.179315/1‑150 (MQ=60)
                                                                             AAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATA                                                                       <  SRR19973080.129723/150‑1 (MQ=60)
                                                                             AAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATA                                                                       <  SRR19973080.351866/150‑1 (MQ=60)
                                                                             AAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATA                                                                       <  SRR19973080.154898/150‑1 (MQ=60)
                                                                             AAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATA                                                                       <  SRR19973080.2314990/150‑1 (MQ=60)
                                                                             AAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATA                                                                       <  SRR19973080.2431834/150‑1 (MQ=60)
                                                                              AACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAC                                                                      >  SRR19973080.3463469/1‑150 (MQ=60)
                                                                                 AAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAA                                                                   >  SRR19973080.2745056/1‑150 (MQ=60)
                                                                                 AAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAA                                                                   >  SRR19973080.4010953/1‑150 (MQ=60)
                                                                                 AAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAA                                                                   >  SRR19973080.4298486/1‑150 (MQ=60)
                                                                                  AGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAA                                                                  <  SRR19973080.1016319/150‑1 (MQ=60)
                                                                                    GGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAAT                                                                <  SRR19973080.178211/150‑1 (MQ=60)
                                                                                       ATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAA                                                             <  SRR19973080.3754958/150‑1 (MQ=60)
                                                                                        TCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAAC                                                            <  SRR19973080.2573218/150‑1 (MQ=60)
                                                                                        TCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAAC                                                            <  SRR19973080.4561564/150‑1 (MQ=60)
                                                                                        TCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAAC                                                            <  SRR19973080.2573201/150‑1 (MQ=60)
                                                                                        TCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGGTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAACAGCAGTGTCAGCAATACGAAAATGAAC                                                            <  SRR19973080.2273664/150‑1 (MQ=60)
                                                                                         CGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAATGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACT                                                           >  SRR19973080.3107309/1‑150 (MQ=60)
                                                                                          GAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACAAAAATGAACTT                                                          <  SRR19973080.3234344/150‑1 (MQ=60)
                                                                                          GAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACAAAAATGAACTT                                                          <  SRR19973080.3234280/150‑1 (MQ=60)
                                                                                          GAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTT                                                          >  SRR19973080.3027646/1‑150 (MQ=60)
                                                                                            AGCCTTGCTAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTAC                                                        <  SRR19973080.1332870/150‑1 (MQ=60)
                                                                                            AGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTAC                                                        <  SRR19973080.588273/150‑1 (MQ=60)
                                                                                            AGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTAC                                                        <  SRR19973080.588218/150‑1 (MQ=60)
                                                                                            AGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTAC                                                        <  SRR19973080.1332824/150‑1 (MQ=60)
                                                                                            AGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTAC                                                        <  SRR19973080.3128972/150‑1 (MQ=60)
                                                                                                TTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAT                                                    <  SRR19973080.2472104/150‑1 (MQ=60)
                                                                                                  GCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTT                                                  >  SRR19973080.10445/1‑150 (MQ=60)
                                                                                                  GCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTT                                                  >  SRR19973080.4534105/1‑150 (MQ=60)
                                                                                                  GCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTT                                                  >  SRR19973080.2291199/1‑150 (MQ=60)
                                                                                                   CAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTA                                                 >  SRR19973080.456225/1‑150 (MQ=60)
                                                                                                      ACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCA                                              >  SRR19973080.4481069/1‑150 (MQ=60)
                                                                                                        GTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGT                                            <  SRR19973080.466301/150‑1 (MQ=60)
                                                                                                        GTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGT                                            >  SRR19973080.2424111/1‑150 (MQ=60)
                                                                                                          AACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGC                                          >  SRR19973080.2594102/1‑150 (MQ=60)
                                                                                                          AACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGC                                          >  SRR19973080.3251491/1‑150 (MQ=60)
                                                                                                            CTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCAC                                        <  SRR19973080.3985505/150‑1 (MQ=60)
                                                                                                             TGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACT                                       <  SRR19973080.3327186/150‑1 (MQ=60)
                                                                                                                   ATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGTATTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGTAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGACAATGAACTTACGCATTTACCAGTGCACTCCAATG                                 >  SRR19973080.2425153/1‑150 (MQ=60)
                                                                                                                          CCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAA                          >  SRR19973080.4425402/1‑150 (MQ=60)
                                                                                                                           CTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGAGCACTCCAATGGTGAGAAA                         >  SRR19973080.1793408/1‑150 (MQ=60)
                                                                                                                            TGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAACGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAC                        <  SRR19973080.3979506/150‑1 (MQ=60)
                                                                                                                            TGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAC                        >  SRR19973080.3680644/1‑150 (MQ=60)
                                                                                                                              CCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGC                      <  SRR19973080.4439982/150‑1 (MQ=60)
                                                                                                                               CACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCG                     >  SRR19973080.3456136/1‑150 (MQ=60)
                                                                                                                               CACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCG                     <  SRR19973080.2996443/150‑1 (MQ=60)
                                                                                                                                   GGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGT                 <  SRR19973080.3482706/150‑1 (MQ=60)
                                                                                                                                    GGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTT                >  SRR19973080.612598/1‑150 (MQ=60)
                                                                                                                                    GGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTT                <  SRR19973080.3988066/150‑1 (MQ=60)
                                                                                                                                    GGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTT                <  SRR19973080.2995949/150‑1 (MQ=60)
                                                                                                                                     GTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTG               >  SRR19973080.1582706/1‑150 (MQ=60)
                                                                                                                                       ATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCG             >  SRR19973080.2622503/1‑150 (MQ=60)
                                                                                                                                       ATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCG             >  SRR19973080.3003407/1‑150 (MQ=60)
                                                                                                                                            TAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACC‑AAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCC       <  SRR19973080.2520439/150‑1 (MQ=60)
                                                                                                                                             AACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCC       <  SRR19973080.1359600/150‑1 (MQ=60)
                                                                                                                                                CTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTT    >  SRR19973080.2556960/1‑150 (MQ=60)
                                                                                                                                                 TGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTA   <  SRR19973080.4074326/150‑1 (MQ=60)
                                                                                                                                                  GACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTAC  >  SRR19973080.2504075/1‑150 (MQ=60)
                                                                                                                                                  GACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTAC  >  SRR19973080.1833201/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
CGCAGCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTAC  >  CP009273/583192‑583487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑