Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 583,341 T→G 100% S33R (AGT→CGT)  ybcH ← PRK09936 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273583,3410TG99.6% 859.3 / ‑5.4 210S33R (AGT→CGT) ybcHPRK09936 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (82/127);  total (82/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

GCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTT  >  CP009273/583196‑583485
                                                                                                                                                 |                                                                                                                                                
gCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGAt                                                                                                                                                <  1:1774880/148‑1 (MQ=255)
    cGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCg                                                                                                                                            <  2:31827/148‑1 (MQ=255)
    cGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCg                                                                                                                                            <  2:31113/148‑1 (MQ=255)
    cGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCg                                                                                                                                            >  2:2699735/1‑148 (MQ=255)
       cccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGtt                                                                                                                                         <  1:731122/148‑1 (MQ=255)
       cccGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGtt                                                                                                                                         <  2:1936983/148‑1 (MQ=255)
         cGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGtttt                                                                                                                                       <  1:3228289/148‑1 (MQ=255)
           cTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtgt                                                                                                                                     >  2:841597/1‑148 (MQ=255)
           cTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTtgt                                                                                                                                     <  1:3422348/148‑1 (MQ=255)
             tAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTgg                                                                                                                                   >  2:1050959/1‑148 (MQ=255)
              aaaCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGt                                                                                                                                  <  2:1093386/148‑1 (MQ=255)
                aCAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTg                                                                                                                                >  2:2498694/1‑148 (MQ=255)
                 cAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGc                                                                                                                               >  1:2763949/1‑148 (MQ=255)
                 cAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGc                                                                                                                               <  2:107382/148‑1 (MQ=255)
                  aaTAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGcc                                                                                                                              >  2:3045505/1‑148 (MQ=255)
                      aCGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGat                                                                                                                          >  2:3499421/1‑148 (MQ=255)
                       cGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGata                                                                                                                         <  1:1764492/148‑1 (MQ=255)
                        gTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGataa                                                                                                                        <  2:1204072/148‑1 (MQ=255)
                         tGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGataat                                                                                                                       <  1:2564728/148‑1 (MQ=255)
                           cgcTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATAc                                                                                                                     <  1:1593767/148‑1 (MQ=255)
                             cTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCt                                                                                                                   <  2:366057/148‑1 (MQ=255)
                                ttCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTc                                                                                                                <  2:2665304/148‑1 (MQ=255)
                                ttCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTc                                                                                                                <  1:2018444/148‑1 (MQ=255)
                                     gctggGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGc                                                                                                           >  1:2645140/1‑148 (MQ=255)
                                       tggGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCaa                                                                                                         <  1:1160981/148‑1 (MQ=255)
                                       tggGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCaa                                                                                                         <  1:2992747/148‑1 (MQ=255)
                                       tggGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCaa                                                                                                         <  1:1405884/148‑1 (MQ=255)
                                       tggGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCaa                                                                                                         <  1:2932091/148‑1 (MQ=255)
                                         ggTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAg                                                                                                       <  2:2259049/148‑1 (MQ=255)
                                           tAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGa                                                                                                     >  1:1486777/1‑148 (MQ=255)
                                            aaaTGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaa                                                                                                    >  2:238800/1‑148 (MQ=255)
                                             aaTGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaa                                                                                                   >  1:104010/1‑148 (MQ=255)
                                               tGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaaaa                                                                                                 <  2:2258838/148‑1 (MQ=255)
                                               tGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaaaa                                                                                                 <  2:748575/148‑1 (MQ=255)
                                               tGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGaaaaa                                                                                                 <  1:21469/148‑1 (MQ=255)
                                                 cATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAgg                                                                                               <  1:1556969/148‑1 (MQ=255)
                                                 cATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAgg                                                                                               <  2:2273537/148‑1 (MQ=255)
                                                   tCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGc                                                                                             <  2:2905993/148‑1 (MQ=255)
                                                   tCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGc                                                                                             <  2:3351719/148‑1 (MQ=255)
                                                    cGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCt                                                                                            >  2:1167664/1‑148 (MQ=255)
                                                      ccGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGa                                                                                          <  2:610576/148‑1 (MQ=255)
                                                         tAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCa                                                                                       >  2:1487499/1‑148 (MQ=255)
                                                         tAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCa                                                                                       >  2:3164124/1‑148 (MQ=255)
                                                               gTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCa                                                                                 <  2:2801703/148‑1 (MQ=255)
                                                                 ccATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgt                                                                               >  2:202669/1‑148 (MQ=255)
                                                                  cagtGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgtg                                                                              <  1:2510674/145‑1 (MQ=255)
                                                                  cATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAgtg                                                                              <  1:1624848/148‑1 (MQ=255)
                                                                     tGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCa                                                                           >  1:2964227/1‑148 (MQ=255)
                                                                         aaaCAAGGGTATTGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCaa                                                                       <  1:2684302/148‑1 (MQ=255)
                                                                          aaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAAt                                                                      <  1:1050959/148‑1 (MQ=255)
                                                                          aaCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAAt                                                                      >  2:2952721/1‑148 (MQ=255)
                                                                           aCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATa                                                                     >  2:2309994/1‑148 (MQ=255)
                                                                           aCAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATa                                                                     >  2:1518238/1‑148 (MQ=255)
                                                                            cAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAc                                                                    >  2:1968591/1‑148 (MQ=255)
                                                                            cAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAc                                                                    >  2:2462455/1‑148 (MQ=255)
                                                                             aaGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACg                                                                   <  1:1420620/148‑1 (MQ=255)
                                                                              aGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGa                                                                  <  2:2479529/148‑1 (MQ=255)
                                                                                ggTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaaa                                                                >  1:3027221/1‑148 (MQ=255)
                                                                                ggTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaaa                                                                >  1:3027950/1‑148 (MQ=255)
                                                                                ggTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGaaa                                                                <  1:2037388/148‑1 (MQ=255)
                                                                                  tATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAAt                                                              >  1:645324/1‑148 (MQ=255)
                                                                                   aTCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATg                                                             <  2:1717330/148‑1 (MQ=255)
                                                                                   aTCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATg                                                             <  2:948596/148‑1 (MQ=255)
                                                                                    tCGAAGCCGTGCAAAGGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGa                                                            <  2:3075511/148‑1 (MQ=255)
                                                                                     cGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGaa                                                           <  2:2668018/148‑1 (MQ=255)
                                                                                      gAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAAc                                                          <  1:1472812/148‑1 (MQ=255)
                                                                                        aGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACtt                                                        >  1:3292207/1‑148 (MQ=255)
                                                                                             tGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCa                                                   <  2:2598384/148‑1 (MQ=255)
                                                                                              gCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAt                                                  <  1:1673204/148‑1 (MQ=255)
                                                                                              gCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCAt                                                  <  1:2506214/148‑1 (MQ=255)
                                                                                                    gTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCa                                            <  1:1839551/148‑1 (MQ=255)
                                                                                                    gTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCa                                            <  1:110121/148‑1 (MQ=255)
                                                                                                     tAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAg                                           >  2:1150230/1‑148 (MQ=255)
                                                                                                         tGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCa                                       <  1:350192/148‑1 (MQ=255)
                                                                                                          gACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCAc                                      >  1:889803/1‑148 (MQ=255)
                                                                                                           aCTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACt                                     <  1:2969361/148‑1 (MQ=255)
                                                                                                               atcaGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCaa                                 <  1:445541/148‑1 (MQ=255)
                                                                                                                tcaGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAAt                                >  1:1475628/1‑148 (MQ=255)
                                                                                                                tcaGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAAt                                <  1:2309994/148‑1 (MQ=255)
                                                                                                                   gCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGt                             <  2:1590048/148‑1 (MQ=255)
                                                                                                                   gCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGt                             >  1:980379/1‑148 (MQ=255)
                                                                                                                    ccccTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTg                            <  2:2195976/148‑1 (MQ=255)
                                                                                                                    ccccTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTg                            <  1:1026247/148‑1 (MQ=255)
                                                                                                                    ccccTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTg                            >  1:1241228/1‑148 (MQ=255)
                                                                                                                    ccccTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTg                            <  2:3312943/148‑1 (MQ=255)
                                                                                                                        tGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGaa                        >  1:2278727/1‑148 (MQ=255)
                                                                                                                         gCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGaaa                       >  1:3067834/1‑148 (MQ=255)
                                                                                                                          ccACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCACTGGTGAGAAAc                      >  1:586783/1‑148 (MQ=255)
                                                                                                                             cTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAAcgcg                   >  2:1806697/1‑148 (MQ=255)
                                                                                                                              tGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgt                  <  2:2964251/148‑1 (MQ=255)
                                                                                                                               gggTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgtt                 >  2:3247756/1‑148 (MQ=255)
                                                                                                                                ggTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgttg                >  1:1117638/1‑148 (MQ=255)
                                                                                                                                ggTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCgttg                >  2:2010101/1‑148 (MQ=255)
                                                                                                                                     cGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTgcg           <  1:1167664/148‑1 (MQ=255)
                                                                                                                                        tAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGctc        >  2:1365512/1‑148 (MQ=255)
                                                                                                                                            cTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAACAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCg    <  2:1132464/148‑1 (MQ=255)
                                                                                                                                            cTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCg    <  1:841597/148‑1 (MQ=255)
                                                                                                                                            cTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCg    <  2:3513159/148‑1 (MQ=255)
                                                                                                                                             tGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGt   <  2:502886/148‑1 (MQ=255)
                                                                                                                                              gACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGtt  >  2:699765/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTT  >  CP009273/583196‑583485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACGTT  >  CP009273/583196‑583490
                                                                                                                                                 |                                                                                                                                                     
GCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCT                                                                                                                                                   <  SRR19973067.1786908/150‑1 (MQ=60)
  TGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCG                                                                                                                                                 <  SRR19973067.1647961/150‑1 (MQ=60)
     GGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTT                                                                                                                                              <  SRR19973067.2206138/150‑1 (MQ=60)
       CCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTT                                                                                                                                            <  SRR19973067.736202/150‑1 (MQ=60)
        CCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTG                                                                                                                                           <  SRR19973067.3285047/150‑1 (MQ=60)
         CGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGT                                                                                                                                          <  SRR19973067.3248554/150‑1 (MQ=60)
         CGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGT                                                                                                                                          <  SRR19973067.1169068/150‑1 (MQ=60)
           CTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGG                                                                                                                                        <  SRR19973067.3444128/150‑1 (MQ=60)
           CTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGG                                                                                                                                        <  SRR19973067.379840/150‑1 (MQ=60)
               AACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGC                                                                                                                                    >  SRR19973067.2781810/1‑150 (MQ=60)
                 CAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCA                                                                                                                                  >  SRR19973067.3102411/1‑150 (MQ=60)
                 CAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCA                                                                                                                                  >  SRR19973067.1684750/1‑150 (MQ=60)
                    TAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGAT                                                                                                                               <  SRR19973067.1481912/150‑1 (MQ=60)
                     AACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATA                                                                                                                              >  SRR19973067.3333879/1‑150 (MQ=60)
                       CGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAAT                                                                                                                            <  SRR19973067.1776492/150‑1 (MQ=60)
                       CGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAAT                                                                                                                            <  SRR19973067.1432209/150‑1 (MQ=60)
                       CGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCAGTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAAT                                                                                                                            <  SRR19973067.2144467/150‑1 (MQ=60)
                         TGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACCAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATAC                                                                                                                          <  SRR19973067.1426587/150‑1 (MQ=60)
                         TGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATAC                                                                                                                          <  SRR19973067.3003920/150‑1 (MQ=60)
                         TGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATAC                                                                                                                          <  SRR19973067.2581480/150‑1 (MQ=60)
                           CGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCT                                                                                                                        <  SRR19973067.1604691/150‑1 (MQ=60)
                           CGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCT                                                                                                                        <  SRR19973067.1605182/150‑1 (MQ=60)
                                TTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCAT                                                                                                                   <  SRR19973067.2032006/150‑1 (MQ=60)
                                   TGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGC                                                                                                                >  SRR19973067.2662222/1‑150 (MQ=60)
                                       TGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAG                                                                                                            <  SRR19973067.1415417/150‑1 (MQ=60)
                                       TGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAG                                                                                                            <  SRR19973067.2950693/150‑1 (MQ=60)
                                       TGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAG                                                                                                            <  SRR19973067.2950709/150‑1 (MQ=60)
                                       TGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAG                                                                                                            <  SRR19973067.1168709/150‑1 (MQ=60)
                                       TGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAG                                                                                                            <  SRR19973067.1168700/150‑1 (MQ=60)
                                       TGGGTAAATGCATCGCCGGAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAG                                                                                                            <  SRR19973067.3011712/150‑1 (MQ=60)
                                         GGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGA                                                                                                          <  SRR19973067.2581219/150‑1 (MQ=60)
                                         GGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGA                                                                                                          >  SRR19973067.1496624/1‑150 (MQ=60)
                                         GGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGA                                                                                                          >  SRR19973067.1496311/1‑150 (MQ=60)
                                         GGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGA                                                                                                          <  SRR19973067.854112/150‑1 (MQ=60)
                                           TAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAA                                                                                                        >  SRR19973067.105047/1‑150 (MQ=60)
                                             AATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAA                                                                                                      <  SRR19973067.3080416/150‑1 (MQ=60)
                                               TGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGG                                                                                                    <  SRR19973067.21794/150‑1 (MQ=60)
                                                 CATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGC                                                                                                  <  SRR19973067.1567575/150‑1 (MQ=60)
                                                               GTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGT                                                                                    <  SRR19973067.3515935/150‑1 (MQ=60)
                                                                TCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTG                                                                                   <  SRR19973067.1783291/150‑1 (MQ=60)
                                                                  CATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTC                                                                                 <  SRR19973067.1635996/150‑1 (MQ=60)
                                                                  CAGTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTC                                                                                 <  SRR19973067.2527151/150‑1 (MQ=60)
                                                                   ATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCA                                                                                >  SRR19973067.2982973/1‑150 (MQ=60)
                                                                   ATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCA                                                                                >  SRR19973067.2982949/1‑150 (MQ=60)
                                                                      GCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCA                                                                             >  SRR19973067.965359/1‑150 (MQ=60)
                                                                        AAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAAT                                                                           >  SRR19973067.1997296/1‑150 (MQ=60)
                                                                        AAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTACATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAAT                                                                           >  SRR19973067.2697705/1‑150 (MQ=60)
                                                                         AAACAAGGGTATTGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATA                                                                          <  SRR19973067.2701512/150‑1 (MQ=60)
                                                                          AACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAC                                                                         <  SRR19973067.2655855/150‑1 (MQ=60)
                                                                          AACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATAC                                                                         <  SRR19973067.1058171/150‑1 (MQ=60)
                                                                             AAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAA                                                                      <  SRR19973067.1430221/150‑1 (MQ=60)
                                                                              AGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAA                                                                     >  SRR19973067.3046495/1‑150 (MQ=60)
                                                                              AGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAA                                                                     >  SRR19973067.3046500/1‑150 (MQ=60)
                                                                              AGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAA                                                                     >  SRR19973067.3047229/1‑150 (MQ=60)
                                                                                GGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAAT                                                                   <  SRR19973067.2051026/150‑1 (MQ=60)
                                                                                GGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAAT                                                                   >  SRR19973067.649690/1‑150 (MQ=60)
                                                                                 GTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATG                                                                  >  SRR19973067.1562203/1‑150 (MQ=60)
                                                                                  TATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGA                                                                 >  SRR19973067.790123/1‑150 (MQ=60)
                                                                                   ATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAA                                                                <  SRR19973067.2478640/150‑1 (MQ=60)
                                                                                   ATCGAAGCCTTGCAAACGTAACAGACTCATCAGCCCCAGCCACTGGGTATCGGTAACCTGACGATCACGGTTTTGTGGAAGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGATGACCAAAAGCACAGTCAGCAATACGAAAATGAA                                                                >  SRR19973067.600734/1‑150 (MQ=60)
                                                                                    TCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAAC                                                               <  SRR19973067.3434211/150‑1 (MQ=60)
                                                                                      GAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTT                                                             <  SRR19973067.3027885/150‑1 (MQ=60)
                                                                                      GAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTT                                                             <  SRR19973067.1482617/150‑1 (MQ=60)
                                                                                      GAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTT                                                             >  SRR19973067.3312911/1‑150 (MQ=60)
                                                                                      GAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTT                                                             >  SRR19973067.2974645/1‑150 (MQ=60)
                                                                                           CTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCA                                                        >  SRR19973067.506332/1‑150 (MQ=60)
                                                                                              GCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTT                                                     <  SRR19973067.2525770/150‑1 (MQ=60)
                                                                                              GCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTT                                                     <  SRR19973067.1684742/150‑1 (MQ=60)
                                                                                              GCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTT                                                     <  SRR19973067.2522658/150‑1 (MQ=60)
                                                                                               CAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTA                                                    >  SRR19973067.698922/1‑150 (MQ=60)
                                                                                               CAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTA                                                    >  SRR19973067.1076751/1‑150 (MQ=60)
                                                                                                 AACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACC                                                  <  SRR19973067.2050147/150‑1 (MQ=60)
                                                                                                  ACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCA                                                 >  SRR19973067.2746036/1‑150 (MQ=60)
                                                                                                  ACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCA                                                 >  SRR19973067.2746007/1‑150 (MQ=60)
                                                                                                    GTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGT                                               <  SRR19973067.111186/150‑1 (MQ=60)
                                                                                                    GTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGT                                               <  SRR19973067.1851442/150‑1 (MQ=60)
                                                                                                    GTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGT                                               <  SRR19973067.112464/150‑1 (MQ=60)
                                                                                                    GTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGT                                               <  SRR19973067.1852098/150‑1 (MQ=60)
                                                                                                        CTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCAC                                           >  SRR19973067.895698/1‑150 (MQ=60)
                                                                                                        CTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCAC                                           >  SRR19973067.1594486/1‑150 (MQ=60)
                                                                                                         TGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACT                                          <  SRR19973067.352614/150‑1 (MQ=60)
                                                                                                           ACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCC                                        <  SRR19973067.2988099/150‑1 (MQ=60)
                                                                                                              CATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAAT                                     >  SRR19973067.1485395/1‑150 (MQ=60)
                                                                                                              CATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAAT                                     >  SRR19973067.1485444/1‑150 (MQ=60)
                                                                                                               ATCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATG                                    <  SRR19973067.448634/150‑1 (MQ=60)
                                                                                                                TCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGG                                   <  SRR19973067.2325208/150‑1 (MQ=60)
                                                                                                                TCAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGG                                   <  SRR19973067.1034180/150‑1 (MQ=60)
                                                                                                                 CAGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGT                                  >  SRR19973067.987109/1‑150 (MQ=60)
                                                                                                                  AGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTG                                 >  SRR19973067.1249986/1‑150 (MQ=60)
                                                                                                                  AGCCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTG                                 >  SRR19973067.1249580/1‑150 (MQ=60)
                                                                                                                    CCCCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAG                               <  SRR19973067.1033305/150‑1 (MQ=60)
                                                                                                                      CCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAA                             >  SRR19973067.2293829/1‑150 (MQ=60)
                                                                                                                      CCTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAA                             >  SRR19973067.2293798/1‑150 (MQ=60)
                                                                                                                       CTGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAA                            >  SRR19973067.3087360/1‑150 (MQ=60)
                                                                                                                        TGCCACTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCACTGGTGAGAAAC                           >  SRR19973067.590532/1‑150 (MQ=60)
                                                                                                                             CTGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTT                      >  SRR19973067.1052662/1‑150 (MQ=60)
                                                                                                                              TGGGTATCGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTG                     >  SRR19973067.1125209/1‑150 (MQ=60)
                                                                                                                                GGTATCGGTAAGCTGTCGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAATCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTT                   <  SRR19973067.1981946/150‑1 (MQ=60)
                                                                                                                                     CGGTAACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCT              <  SRR19973067.1175417/150‑1 (MQ=60)
                                                                                                                                         AACCTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCC          <  SRR19973067.1200056/150‑1 (MQ=60)
                                                                                                                                            CTGACGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTT       <  SRR19973067.847309/150‑1 (MQ=60)
                                                                                                                                                CGATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACGT   >  SRR19973067.1080163/1‑150 (MQ=60)
                                                                                                                                                 GATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACGTT  <  SRR19973067.1545044/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
GCTGCGGCCCGCTTAAACAATAACGTGCGCTGTTCTGGCTGGGTAAATGCATCGCCGTAACGGGTCCATTGCAAAACAAGGGTATCGAAGCCTTGCAAACGTAACTGACTCATCAGCCCCTGCCACTGGGTATCGGTAACCTGACTATCTCGGTTTTGTGGTTGCCAGATAATACCTTTCATCGCAAAGGAAAAAGGGCTGACCAAAAGCAGTGTCAGCAATACGAAAATGAACTTACGCATTTACCAGTGCACTCCAATGGTGAGAAACGCGTTGTTGCGCTCTCCGTTACGTT  >  CP009273/583196‑583490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑