Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 549,397 G→T 93.2% intergenic (‑1/+2) lpxH ← / ← ppiB UDP‑2,3‑diacylglucosamine pyrophosphohydrolase/peptidyl‑prolyl cis‑trans isomerase B (rotamase B)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273549,3970GT93.2% 815.7 / 42.8 260intergenic (‑1/+2)lpxH/ppiBUDP‑2,3‑diacylglucosamine pyrophosphohydrolase/peptidyl‑prolyl cis‑trans isomerase B (rotamase B)
Reads supporting (aligned to +/- strand):  ref base G (11/7);  new base T (122/120);  total (133/127)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 260 total aligned reads displayed.

GTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACC  >  CP009273/549250‑549545
                                                                                                                                                   |                                                                                                                                                    
gtcgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTAt                                                                                                                                                    >  2:1905254/1‑150 (MQ=255)
gtcgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGAt                                                                                                                                                    <  2:1784139/150‑1 (MQ=255)
gtcgCCAATCCATGCTTCAAACAGAGCGCCACGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTAt                                                                                                                                                    <  2:1348108/150‑1 (MQ=255)
 tcgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCACTAAAGAGTGTCGCCACTAtt                                                                                                                                                   >  2:1461198/1‑150 (MQ=255)
 tcgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTAtt                                                                                                                                                   >  2:585365/1‑150 (MQ=255)
    ccAATCCATGCTTAAAACCGATCGCCAATAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACt                                                                                                                                                <  1:1809034/150‑1 (MQ=255)
         ccATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTa                                                                                                                                           <  1:4936937/150‑1 (MQ=255)
           aTGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTCAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAAc                                                                                                                                         >  1:4861101/1‑150 (MQ=255)
                 cAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCAc                                                                                                                                   <  1:1016686/150‑1 (MQ=255)
                 cAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCAc                                                                                                                                   <  2:2301462/150‑1 (MQ=255)
                 cAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCAc                                                                                                                                   <  2:4884628/150‑1 (MQ=255)
                 cAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCAc                                                                                                                                   >  1:522128/1‑150 (MQ=255)
                    aCAGATCGCCATGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCAGCTAAAAAACGCAGAAAACCGGCGGTGATCGCAGGTTCTTCCACGAAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTTACGGTCACGCt                                                                                                                                <  2:4327714/150‑1 (MQ=255)
                    aCAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCt                                                                                                                                >  2:4788612/1‑150 (MQ=255)
                     cAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCtt                                                                                                                               >  2:2680595/1‑150 (MQ=255)
                      aGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCttt                                                                                                                              >  1:2480509/1‑150 (MQ=255)
                       gATCGCCAAGAATATACAGCGCGTCGGCCTGGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTc                                                                                                                             <  1:5027914/150‑1 (MQ=255)
                        aTCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCa                                                                                                                            <  1:4716668/150‑1 (MQ=255)
                         tCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCaa                                                                                                                           >  1:4614295/1‑150 (MQ=255)
                           gCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATg                                                                                                                         >  1:32467/1‑150 (MQ=255)
                             cAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGAt                                                                                                                       <  2:4054598/150‑1 (MQ=255)
                             cAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGAt                                                                                                                       >  1:3214003/1‑150 (MQ=255)
                              aaGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATa                                                                                                                      >  1:2280870/1‑150 (MQ=255)
                              aaGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCGAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATa                                                                                                                      >  1:3752655/1‑150 (MQ=255)
                                 aaTATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACg                                                                                                                   <  2:2915338/150‑1 (MQ=255)
                                  atatACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGt                                                                                                                  >  1:3593427/1‑150 (MQ=255)
                                   tataCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtc                                                                                                                 <  2:3130066/150‑1 (MQ=255)
                                             gTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGc                                                                                                       >  2:1085203/1‑150 (MQ=255)
                                             gTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGc                                                                                                       <  1:2608905/150‑1 (MQ=255)
                                                ggCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACg                                                                                                    >  2:1434745/1‑150 (MQ=255)
                                                ggCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACg                                                                                                    >  2:536698/1‑150 (MQ=255)
                                                ggCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACg                                                                                                    >  2:2358101/1‑150 (MQ=255)
                                                   cTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAACCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTcc                                                                                                 <  1:588579/150‑1 (MQ=255)
                                                   cTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTcc                                                                                                 >  2:2544938/1‑150 (MQ=255)
                                                   cTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTcc                                                                                                 >  2:579169/1‑150 (MQ=255)
                                                     tGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCATg                                                                                               <  1:4110154/150‑1 (MQ=255)
                                                       cGGGCGTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGt                                                                                             <  2:791277/150‑1 (MQ=255)
                                                        gggCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTg                                                                                            <  1:1404577/150‑1 (MQ=255)
                                                           cTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCAt                                                                                         >  2:3342485/1‑150 (MQ=255)
                                                            ttCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATa                                                                                        >  2:2870737/1‑150 (MQ=255)
                                                             tCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATAc                                                                                       >  2:1978571/1‑150 (MQ=255)
                                                              cccccGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATAcc                                                                                      <  1:1765179/150‑1 (MQ=255)
                                                              cccccGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATAcc                                                                                      >  2:1686636/1‑150 (MQ=255)
                                                              cccccGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATAcc                                                                                      <  2:128091/150‑1 (MQ=255)
                                                               ccccGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCg                                                                                     <  1:4304357/150‑1 (MQ=255)
                                                                   gCTAAAAAACGCAGAAACCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTAc                                                                                 <  1:896296/150‑1 (MQ=255)
                                                                    cTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACg                                                                                <  2:132225/150‑1 (MQ=255)
                                                                     tAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGa                                                                               >  2:3593105/1‑150 (MQ=255)
                                                                      aaaaaaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTCTTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGAc                                                                              >  2:1112796/1‑150 (MQ=255)
                                                                      aaaaaaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGAc                                                                              >  2:1113007/1‑150 (MQ=255)
                                                                       aaaaaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGAcc                                                                             >  2:4100606/1‑150 (MQ=255)
                                                                       aaaaaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTGTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGAcc                                                                             >  1:3872716/1‑150 (MQ=255)
                                                                          aaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGt                                                                          <  1:3729115/150‑1 (MQ=255)
                                                                            cGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTg                                                                        >  2:2093274/1‑150 (MQ=255)
                                                                              cAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCa                                                                      >  2:3401286/1‑150 (MQ=255)
                                                                               aGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCaa                                                                     >  2:327348/1‑150 (MQ=255)
                                                                               aGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCaa                                                                     <  1:3290182/150‑1 (MQ=255)
                                                                                gAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATACAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAac                                                                    <  2:5126091/150‑1 (MQ=255)
                                                                                gAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAac                                                                    <  2:2600144/150‑1 (MQ=255)
                                                                                 aaaaCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAaca                                                                   <  2:840861/150‑1 (MQ=255)
                                                                                  aaaCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAacac                                                                  >  2:1799788/1‑150 (MQ=255)
                                                                                      cggcggTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCttt                                                              <  1:1167048/150‑1 (MQ=255)
                                                                                       ggcggTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCACTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTg                                                             >  2:1555443/1‑150 (MQ=255)
                                                                                         cggTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGAt                                                           <  1:3530003/150‑1 (MQ=255)
                                                                                           gTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGAttt                                                         <  1:2689037/150‑1 (MQ=255)
                                                                                             gATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTg                                                       <  1:131653/150‑1 (MQ=255)
                                                                                              aTCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGt                                                      >  2:2726723/1‑150 (MQ=255)
                                                                                                 gCCGGTTCTTCCTCGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTa                                                   <  2:3667892/150‑1 (MQ=255)
                                                                                                   cGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTCCGACCGGTTGCAACACCTTTGATTTTGTCTAcc                                                 >  2:3536511/1‑150 (MQ=255)
                                                                                                     gTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCAc                                               <  2:1706542/150‑1 (MQ=255)
                                                                                                        cttcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTc                                            <  2:3823829/150‑1 (MQ=255)
                                                                                                           ccACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCAt                                         <  2:5137867/150‑1 (MQ=255)
                                                                                                            cACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATg                                        <  1:1572363/150‑1 (MQ=255)
                                                                                                              cGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGcc                                      >  1:2393168/1‑150 (MQ=255)
                                                                                                              cGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGcc                                      >  1:4974336/1‑150 (MQ=255)
                                                                                                                 agagATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTc                                   >  1:2170321/1‑150 (MQ=255)
                                                                                                                 agagATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTc                                   >  1:2170814/1‑150 (MQ=255)
                                                                                                                  gagaTGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCa                                  <  1:3481974/150‑1 (MQ=255)
                                                                                                                   agaTGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCaa                                 <  1:828844/150‑1 (MQ=255)
                                                                                                                     aTGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAAcc                               >  2:3046759/1‑150 (MQ=255)
                                                                                                                     aTGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAAcc                               >  2:3046877/1‑150 (MQ=255)
                                                                                                                     aTGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAAcc                               >  1:2101706/1‑150 (MQ=255)
                                                                                                                       gtaGATCTGCAATAAAGAGTGTCGCCTCTATTACTCGCTAACGGTCACGCTTTCTATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCAc                             <  2:4202675/148‑1 (MQ=255)
                                                                                                                       gAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCAc                             >  2:408141/1‑150 (MQ=255)
                                                                                                                        aaGATCAGCAATAAAGAGTGTCCCCACGATTACTCGCTAACGGACACGCTTTAAATGATAACGTCTTCTTTTGTCACGTACTGGTTCATACCGCTACGACCGGTTGCAACAACTTTGATTTTGTCTACCACGTCCAAGCAGTCAACCACt                            >  2:930769/1‑150 (MQ=255)
                                                                                                                                cAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCaaa                    >  1:39065/1‑150 (MQ=255)
                                                                                                                                 aaTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAac                   <  1:4478847/150‑1 (MQ=255)
                                                                                                                                 aaTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCAGGCCGTCAACCACTTCAGCAAac                   >  2:4394426/1‑150 (MQ=255)
                                                                                                                                 aaTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTGTGATTTTTTCTACCACGTCCATGCCGTCAACCACTTCAGCAAac                   >  2:4394626/1‑150 (MQ=255)
                                                                                                                                  aTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAaca                  <  1:2822132/150‑1 (MQ=255)
                                                                                                                                  aTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCCTCAACCACTTCAGCAAaca                  >  2:2213350/1‑150 (MQ=255)
                                                                                                                                    aaaGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACg                >  1:1708918/1‑150 (MQ=255)
                                                                                                                                    aaaGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACg                <  2:1222914/150‑1 (MQ=255)
                                                                                                                                      agagTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCa              <  1:1830181/150‑1 (MQ=255)
                                                                                                                                       gagTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAg             <  1:2897014/150‑1 (MQ=255)
                                                                                                                                         gtgtCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTATACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTa           >  1:4324275/1‑150 (MQ=255)
                                                                                                                                          tgtCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGAGTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAg          >  2:2891473/1‑150 (MQ=255)
                                                                                                                                               ccACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCa     >  1:932976/1‑150 (MQ=255)
                                                                                                                                                cACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCaa    <  2:1067340/150‑1 (MQ=255)
                                                                                                                                                  cGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAAcc  >  2:4956650/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACC  >  CP009273/549250‑549545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCT  >  CP009273/549248‑549546
                                                                                                                                                     |                                                                                                                                                     
TCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACT                                                                                                                                                       <  SRR19973083.433375/150‑1 (MQ=60)
TCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACT                                                                                                                                                       <  SRR19973083.433435/150‑1 (MQ=60)
      CCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACT                                                                                                                                                 <  SRR19973083.1818191/150‑1 (MQ=60)
      CCAATCCATGCTTAAAACCGATCGCCAATAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACT                                                                                                                                                 <  SRR19973083.1818118/150‑1 (MQ=60)
       CAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTC                                                                                                                                                >  SRR19973083.2468899/1‑150 (MQ=60)
           CCATGCTTCAAACAGATCGCCAAGAATATACCGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTA                                                                                                                                            <  SRR19973083.4958192/150‑1 (MQ=60)
           CCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTA                                                                                                                                            <  SRR19973083.4958166/150‑1 (MQ=60)
            CATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAA                                                                                                                                           >  SRR19973083.4974687/1‑150 (MQ=60)
             ATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTCAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAAC                                                                                                                                          >  SRR19973083.4881971/1‑150 (MQ=60)
             ATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAAC                                                                                                                                          <  SRR19973083.5009789/150‑1 (MQ=60)
               GCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGG                                                                                                                                        <  SRR19973083.1671143/150‑1 (MQ=60)
               GCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGG                                                                                                                                        <  SRR19973083.3882042/150‑1 (MQ=60)
                 TTCAAACAGATCGCCAAGAATATACCGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTC                                                                                                                                      <  SRR19973083.3992943/150‑1 (MQ=60)
                 TTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTC                                                                                                                                      >  SRR19973083.427763/1‑150 (MQ=60)
                   CAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCAC                                                                                                                                    >  SRR19973083.1749126/1‑150 (MQ=60)
                   CAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCAC                                                                                                                                    <  SRR19973083.1021778/150‑1 (MQ=60)
                   CAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCAC                                                                                                                                    >  SRR19973083.524692/1‑150 (MQ=60)
                        AGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTT                                                                                                                               >  SRR19973083.2493191/1‑150 (MQ=60)
                        AGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTT                                                                                                                               >  SRR19973083.4489807/1‑150 (MQ=60)
                         GATCGCCAAGAATATACAGCGCGTCGGCCTGGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTC                                                                                                                              <  SRR19973083.5049471/150‑1 (MQ=60)
                          ATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCA                                                                                                                             <  SRR19973083.4736844/150‑1 (MQ=60)
                          ATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCA                                                                                                                             <  SRR19973083.4733897/150‑1 (MQ=60)
                           TCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAA                                                                                                                            >  SRR19973083.4634217/1‑150 (MQ=60)
                            CGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAAT                                                                                                                           >  SRR19973083.1615096/1‑150 (MQ=60)
                             GCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATG                                                                                                                          >  SRR19973083.32650/1‑150 (MQ=60)
                             GCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATG                                                                                                                          >  SRR19973083.32716/1‑150 (MQ=60)
                               CAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGAT                                                                                                                        <  SRR19973083.372481/150‑1 (MQ=60)
                               CAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGAT                                                                                                                        >  SRR19973083.3229255/1‑150 (MQ=60)
                               CAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGAT                                                                                                                        >  SRR19973083.3229243/1‑150 (MQ=60)
                                AAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATA                                                                                                                       >  SRR19973083.2230423/1‑150 (MQ=60)
                                AAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATA                                                                                                                       >  SRR19973083.2292542/1‑150 (MQ=60)
                                AAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCGAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATA                                                                                                                       >  SRR19973083.3769657/1‑150 (MQ=60)
                                    ATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGT                                                                                                                   >  SRR19973083.2115025/1‑150 (MQ=60)
                                    ATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGT                                                                                                                   >  SRR19973083.3609889/1‑150 (MQ=60)
                                     TATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTC                                                                                                                  >  SRR19973083.1241133/1‑150 (MQ=60)
                                      ATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCT                                                                                                                 <  SRR19973083.4948975/150‑1 (MQ=60)
                                         CAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCT                                                                                                              >  SRR19973083.5074757/1‑150 (MQ=60)
                                          AGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTT                                                                                                             >  SRR19973083.2814204/1‑150 (MQ=60)
                                               GTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAACCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGC                                                                                                        <  SRR19973083.366718/150‑1 (MQ=60)
                                               GTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGC                                                                                                        >  SRR19973083.540821/1‑150 (MQ=60)
                                               GTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGC                                                                                                        <  SRR19973083.2622030/150‑1 (MQ=60)
                                                  GGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACG                                                                                                     >  SRR19973083.913793/1‑150 (MQ=60)
                                                     CTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAACCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCC                                                                                                  <  SRR19973083.591540/150‑1 (MQ=60)
                                                     CTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCC                                                                                                  >  SRR19973083.4551515/1‑150 (MQ=60)
                                                       TGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTG                                                                                                <  SRR19973083.2770195/150‑1 (MQ=60)
                                                       TGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCATG                                                                                                <  SRR19973083.4128354/150‑1 (MQ=60)
                                                        GCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGG                                                                                               >  SRR19973083.180900/1‑150 (MQ=60)
                                                          GGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTG                                                                                             <  SRR19973083.1411588/150‑1 (MQ=60)
                                                           GGCTTCCCCCGCTAAAAAACGCATAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGC                                                                                            <  SRR19973083.1377999/150‑1 (MQ=60)
                                                           GGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGC                                                                                            <  SRR19973083.2252889/150‑1 (MQ=60)
                                                                CCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACC                                                                                       <  SRR19973083.1773909/150‑1 (MQ=60)
                                                                 CCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCG                                                                                      <  SRR19973083.4323080/150‑1 (MQ=60)
                                                                     GCTAAAAAACGCAGAAACCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTAC                                                                                  <  SRR19973083.900548/150‑1 (MQ=60)
                                                                      CTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACG                                                                                 <  SRR19973083.3186916/150‑1 (MQ=60)
                                                                      CTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACG                                                                                 >  SRR19973083.3599829/1‑150 (MQ=60)
                                                                        AAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGAC                                                                               >  SRR19973083.3122829/1‑150 (MQ=60)
                                                                         AAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAATGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACC                                                                              >  SRR19973083.3890555/1‑150 (MQ=60)
                                                                         AAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTGTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACC                                                                              >  SRR19973083.3890018/1‑150 (MQ=60)
                                                                            AACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGT                                                                           <  SRR19973083.3746047/150‑1 (MQ=60)
                                                                            AACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGT                                                                           <  SRR19973083.3746062/150‑1 (MQ=60)
                                                                             ACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTT                                                                          <  SRR19973083.3779730/150‑1 (MQ=60)
                                                                                 AGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAA                                                                      <  SRR19973083.3305662/150‑1 (MQ=60)
                                                                                 AGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAA                                                                      <  SRR19973083.3305617/150‑1 (MQ=60)
                                                                                  GAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAAC                                                                     <  SRR19973083.5143559/150‑1 (MQ=60)
                                                                                        CGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTT                                                               <  SRR19973083.1172848/150‑1 (MQ=60)
                                                                                        CGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTT                                                               >  SRR19973083.1224888/1‑150 (MQ=60)
                                                                                           CGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGAT                                                            <  SRR19973083.3546205/150‑1 (MQ=60)
                                                                                           CGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGAT                                                            <  SRR19973083.2890074/150‑1 (MQ=60)
                                                                                             GTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTT                                                          <  SRR19973083.4249350/150‑1 (MQ=60)
                                                                                             GTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTT                                                          >  SRR19973083.2701607/1‑150 (MQ=60)
                                                                                             GTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTT                                                          <  SRR19973083.2702518/150‑1 (MQ=60)
                                                                                             GTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTT                                                          >  SRR19973083.1938113/1‑150 (MQ=60)
                                                                                               GATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTG                                                        <  SRR19973083.132484/150‑1 (MQ=60)
                                                                                                  CGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCT                                                     <  SRR19973083.3408863/150‑1 (MQ=60)
                                                                                                    CCGGTTCTTCCACGCAGAGATGACGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTAC                                                   <  SRR19973083.815616/150‑1 (MQ=60)
                                                                                                       GTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCAC                                                <  SRR19973083.2386635/150‑1 (MQ=60)
                                                                                                        TTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACG                                               >  SRR19973083.4055625/1‑150 (MQ=60)
                                                                                                            TCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCA                                           <  SRR19973083.2475360/150‑1 (MQ=60)
                                                                                                             CCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCAT                                          <  SRR19973083.2574280/150‑1 (MQ=60)
                                                                                                              CACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATG                                         <  SRR19973083.1580258/150‑1 (MQ=60)
                                                                                                              CACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATG                                         <  SRR19973083.1792283/150‑1 (MQ=60)
                                                                                                                TGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCC                                       >  SRR19973083.4768432/1‑150 (MQ=60)
                                                                                                                CGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCC                                       >  SRR19973083.4995703/1‑150 (MQ=60)
                                                                                                                CGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCC                                       >  SRR19973083.2405474/1‑150 (MQ=60)
                                                                                                                CGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCC                                       <  SRR19973083.2547019/150‑1 (MQ=60)
                                                                                                                   AGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTC                                    >  SRR19973083.2181694/1‑150 (MQ=60)
                                                                                                                   AGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTC                                    >  SRR19973083.2181545/1‑150 (MQ=60)
                                                                                                                   AGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTC                                    >  SRR19973083.2181199/1‑150 (MQ=60)
                                                                                                                    GAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCA                                   <  SRR19973083.3497892/150‑1 (MQ=60)
                                                                                                                     AGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAA                                  <  SRR19973083.832777/150‑1 (MQ=60)
                                                                                                                     AGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAA                                  <  SRR19973083.836841/150‑1 (MQ=60)
                                                                                                                       ATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACC                                >  SRR19973083.2112403/1‑150 (MQ=60)
                                                                                                                       ATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACC                                >  SRR19973083.2112348/1‑150 (MQ=60)
                                                                                                                       ATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGACCATGCCGTCAACC                                >  SRR19973083.2112150/1‑150 (MQ=60)
                                                                                                                        TGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCA                               <  SRR19973083.3497964/150‑1 (MQ=60)
                                                                                                                         GAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCAC                              >  SRR19973083.4653717/1‑150 (MQ=60)
                                                                                                                          AAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACT                             >  SRR19973083.1519135/1‑150 (MQ=60)
                                                                                                                           AGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTT                            >  SRR19973083.331705/1‑150 (MQ=60)
                                                                                                                            GATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTC                           <  SRR19973083.110972/150‑1 (MQ=60)
                                                                                                                              TCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAG                         >  SRR19973083.86235/1‑150 (MQ=60)
                                                                                                                              TCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAG                         <  SRR19973083.1004132/150‑1 (MQ=60)
                                                                                                                                  CAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAA                     >  SRR19973083.39376/1‑150 (MQ=60)
                                                                                                                                   AATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAAC                    <  SRR19973083.4765202/150‑1 (MQ=60)
                                                                                                                                   AATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAAC                    <  SRR19973083.4498298/150‑1 (MQ=60)
                                                                                                                                   AATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAAC                    >  SRR19973083.586398/1‑150 (MQ=60)
                                                                                                                                    ATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACA                   <  SRR19973083.2836100/150‑1 (MQ=60)
                                                                                                                                      AAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACG                 >  SRR19973083.1717321/1‑150 (MQ=60)
                                                                                                                                        AGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCA               <  SRR19973083.3568282/150‑1 (MQ=60)
                                                                                                                                        AGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCA               <  SRR19973083.1839393/150‑1 (MQ=60)
                                                                                                                                        AGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCA               <  SRR19973083.4439279/150‑1 (MQ=60)
                                                                                                                                         GAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAG              <  SRR19973083.2911215/150‑1 (MQ=60)
                                                                                                                                           GTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTA            >  SRR19973083.4343008/1‑150 (MQ=60)
                                                                                                                                           GTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTATACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTA            >  SRR19973083.4343044/1‑150 (MQ=60)
                                                                                                                                                 CCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCA      <  SRR19973083.1521381/150‑1 (MQ=60)
                                                                                                                                                 CCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCA      >  SRR19973083.937512/1‑150 (MQ=60)
                                                                                                                                                     TATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCT  <  SRR19973083.1397082/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TCGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCT  >  CP009273/549248‑549546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑