Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 549,397 G→T 100% intergenic (‑1/+2) lpxH ← / ← ppiB UDP‑2,3‑diacylglucosamine pyrophosphohydrolase/peptidyl‑prolyl cis‑trans isomerase B (rotamase B)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273549,3970GT100.0% 616.4 / NA 188intergenic (‑1/+2)lpxH/ppiBUDP‑2,3‑diacylglucosamine pyrophosphohydrolase/peptidyl‑prolyl cis‑trans isomerase B (rotamase B)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (92/96);  total (92/96)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 188 total aligned reads displayed.

CGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCC  >  CP009273/549252‑549541
                                                                                                                                                 |                                                                                                                                                
cgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCACTAAAGAGTGTCGCCACTAt                                                                                                                                                >  2:1916231/1‑148 (MQ=255)
  ccAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTa                                                                                                                                              <  2:2937195/148‑1 (MQ=255)
  ccAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGATAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTa                                                                                                                                              <  2:2399981/148‑1 (MQ=255)
  caaaTCCATGCTTCAAACAGATCGCCAATAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTa                                                                                                                                              <  1:2536538/146‑1 (MQ=255)
    aaTCCATGCTTCAAACAGATCGCCAAGAATATACCGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACt                                                                                                                                            <  2:446956/148‑1 (MQ=255)
     aTCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTc                                                                                                                                           >  1:3527100/1‑148 (MQ=255)
     aTCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTc                                                                                                                                           >  2:1192623/1‑148 (MQ=255)
      tCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCg                                                                                                                                          >  2:2195024/1‑148 (MQ=255)
       ccATGCTTCAAACAGATCGCCAAGAATATACCGCGCGGCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCACAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGc                                                                                                                                         <  1:3354067/148‑1 (MQ=255)
       ccATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGc                                                                                                                                         >  2:3355728/1‑148 (MQ=255)
         aTGCTACAAACAGAACCCCAACAATATCCCGCGCGGCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCCCAAACCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTa                                                                                                                                       <  1:912575/148‑1 (MQ=255)
          tGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCtca                                                                                                                                      >  2:3398480/1‑146 (MQ=255)
          tGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTaa                                                                                                                                      <  2:3246086/148‑1 (MQ=255)
                aaaCAGATCGCCAAGAATATACCGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCa                                                                                                                                <  2:3169119/148‑1 (MQ=255)
                 aaCAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCAc                                                                                                                               >  1:1361820/1‑148 (MQ=255)
                    aGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCt                                                                                                                            <  1:2252614/148‑1 (MQ=255)
                    aGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCt                                                                                                                            <  1:3165782/148‑1 (MQ=255)
                     gATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCtt                                                                                                                           <  1:1998739/148‑1 (MQ=255)
                      aTCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCttt                                                                                                                          >  1:1564122/1‑148 (MQ=255)
                         gCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCaa                                                                                                                       <  2:194784/148‑1 (MQ=255)
                         gCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCaa                                                                                                                       <  1:1023935/148‑1 (MQ=255)
                          ccAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAAt                                                                                                                      <  1:785039/148‑1 (MQ=255)
                          ccAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAAt                                                                                                                      >  2:939037/1‑148 (MQ=255)
                          ccAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGAGTCGCCACTATAACTCGCTAACGGTCACGCTTTaaaa                                                                                                                      >  2:100554/1‑147 (MQ=255)
                            aaGAATATACCGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGa                                                                                                                    <  1:3067854/148‑1 (MQ=255)
                              gAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATa                                                                                                                  <  2:1782293/148‑1 (MQ=255)
                                 tataCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACg                                                                                                               >  1:2840278/1‑148 (MQ=255)
                                  ataCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGt                                                                                                              <  1:2362535/148‑1 (MQ=255)
                                  ataCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGt                                                                                                              <  1:2551667/148‑1 (MQ=255)
                                  ataCAGCGCGTCGGCCTTGCGGGCTTCCCCCGATAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGt                                                                                                              <  1:1797354/148‑1 (MQ=255)
                                   taCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtc                                                                                                             <  2:3005632/148‑1 (MQ=255)
                                    aCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtct                                                                                                            <  2:3476057/148‑1 (MQ=255)
                                    aCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtct                                                                                                            >  2:1741126/1‑148 (MQ=255)
                                    aCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtct                                                                                                            >  1:1438045/1‑148 (MQ=255)
                                     cAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtctt                                                                                                           >  2:19817/1‑148 (MQ=255)
                                     cAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtctt                                                                                                           <  1:3219949/148‑1 (MQ=255)
                                      aGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtcttc                                                                                                          <  1:61097/148‑1 (MQ=255)
                                      aGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtcttc                                                                                                          <  2:1326951/148‑1 (MQ=255)
                                      aGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtcttc                                                                                                          <  1:1793183/148‑1 (MQ=255)
                                      aGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtcttc                                                                                                          >  1:2092713/1‑148 (MQ=255)
                                       gcgcGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtcttct                                                                                                         >  1:1630362/1‑148 (MQ=255)
                                       gcgcGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtcttct                                                                                                         >  1:1628894/1‑148 (MQ=255)
                                        cgcgTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTcttctt                                                                                                        <  1:3264786/148‑1 (MQ=255)
                                        cgcgTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTcttctt                                                                                                        >  1:1545704/1‑148 (MQ=255)
                                         gcgTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCttt                                                                                                       <  2:2628864/148‑1 (MQ=255)
                                           gTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCACAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTg                                                                                                     <  1:2727953/148‑1 (MQ=255)
                                            tCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTgg                                                                                                    <  1:3499557/148‑1 (MQ=255)
                                                ccTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACg                                                                                                >  2:1575929/1‑148 (MQ=255)
                                                ccTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACCCAGAGATGTAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACg                                                                                                >  1:1331394/1‑148 (MQ=255)
                                                  ttGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTc                                                                                              <  2:835804/148‑1 (MQ=255)
                                                      gggCTTCCCACGCTAAAAAACTCAGAAAACCGTCGGTGATCGCCGTTTCTTCCACGCAGAGATGACGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTgg                                                                                          <  2:1625540/148‑1 (MQ=255)
                                                         cTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGc                                                                                       <  2:2951206/148‑1 (MQ=255)
                                                           tCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCAt                                                                                     >  1:3113023/1‑148 (MQ=255)
                                                            cccccGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATa                                                                                    <  1:181931/148‑1 (MQ=255)
                                                                       aaaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGAcc                                                                         >  2:3095622/1‑148 (MQ=255)
                                                                          cGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTCCGACCGGt                                                                      >  2:2618903/1‑148 (MQ=255)
                                                                             aGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGc                                                                   >  2:699981/1‑148 (MQ=255)
                                                                                    cggcggTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCt                                                            <  2:3043760/148‑1 (MQ=255)
                                                                                     ggcggTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCtt                                                           <  1:1476609/148‑1 (MQ=255)
                                                                                      gcggTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTACTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCttt                                                          <  2:1039928/148‑1 (MQ=255)
                                                                                          tGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGCGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGAtt                                                      <  1:3363234/148‑1 (MQ=255)
                                                                                               gCCGGTTCTTCCACGCAGAGATTAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTc                                                 <  2:3336337/148‑1 (MQ=255)
                                                                                                  ggTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTAc                                              <  1:1576462/148‑1 (MQ=255)
                                                                                                    ttcttcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCa                                            >  2:1404178/1‑148 (MQ=255)
                                                                                                    ttcttcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCa                                            >  1:2845696/1‑148 (MQ=255)
                                                                                                     tcttcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCAc                                           >  1:3461433/1‑148 (MQ=255)
                                                                                                     tcttcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCAc                                           >  1:586296/1‑148 (MQ=255)
                                                                                                      cttcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACg                                          >  1:1195960/1‑148 (MQ=255)
                                                                                                      cttcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACg                                          <  1:692361/148‑1 (MQ=255)
                                                                                                       ttcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGt                                         <  2:2092713/148‑1 (MQ=255)
                                                                                                        tcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTc                                        <  2:21945/148‑1 (MQ=255)
                                                                                                         ccACGCAGAGATGAAGATCTGCAATACAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTcc                                       <  2:1883869/148‑1 (MQ=255)
                                                                                                         ccACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTcc                                       >  2:2425484/1‑148 (MQ=255)
                                                                                                         ccACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTcc                                       >  2:2425486/1‑148 (MQ=255)
                                                                                                          cACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCa                                      >  2:1441011/1‑148 (MQ=255)
                                                                                                           aCGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCAt                                     >  2:665251/1‑148 (MQ=255)
                                                                                                           aCGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCAt                                     >  1:2032161/1‑148 (MQ=255)
                                                                                                                gagaTGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGt                                <  1:2482007/148‑1 (MQ=255)
                                                                                                                gagaTGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGt                                >  1:1321251/1‑148 (MQ=255)
                                                                                                                  gaTGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCa                              >  1:1327927/1‑148 (MQ=255)
                                                                                                                   aTGAAGATCTGCAATAAAGAGTGTCGCCACTCTTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCaa                             <  2:2724938/148‑1 (MQ=255)
                                                                                                                    tGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAAc                            <  1:2124730/148‑1 (MQ=255)
                                                                                                                       aGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCAc                         <  2:1135467/148‑1 (MQ=255)
                                                                                                                         aTCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACtt                       >  1:171484/1‑148 (MQ=255)
                                                                                                                         aTCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACtt                       >  1:3139902/1‑148 (MQ=255)
                                                                                                                         aTCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACtt                       >  2:1509914/1‑148 (MQ=255)
                                                                                                                            tGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAg                    <  1:2724133/148‑1 (MQ=255)
                                                                                                                            tGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAg                    <  1:2724046/148‑1 (MQ=255)
                                                                                                                               aaTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCaa                 <  2:2252114/148‑1 (MQ=255)
                                                                                                                               aaTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCaa                 <  2:1070359/148‑1 (MQ=255)
                                                                                                                                   aaGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAacac             >  1:1883501/1‑148 (MQ=255)
                                                                                                                                    agagTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAAAGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACg            >  2:118656/1‑148 (MQ=255)
                                                                                                                                      agTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCa          <  2:364687/148‑1 (MQ=255)
                                                                                                                                      agTGTCGCATCTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCa          <  2:364630/148‑1 (MQ=255)
                                                                                                                                        tgtCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGt        >  2:3002112/1‑148 (MQ=255)
                                                                                                                                             ccACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGccc   <  1:1644156/148‑1 (MQ=255)
                                                                                                                                             ccACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGccc   <  1:3355737/148‑1 (MQ=255)
                                                                                                                                             ccACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGccc   >  2:1324927/1‑148 (MQ=255)
                                                                                                                                              cACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGcccc  >  2:1850515/1‑148 (MQ=255)
                                                                                                                                              cACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGcccc  >  2:2841133/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCC  >  CP009273/549252‑549541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCT  >  CP009273/549249‑549546
                                                                                                                                                    |                                                                                                                                                     
CGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTACCCCGCTAAAAAACGCAGAAAACCTGCGGTGATCGCCGGTTCTTCAACGCAGAGAAGAATATATGCAATAAAGAGTGTCGACACAA                                                                                                                                                      >  SRR19973066.679641/1‑150 (MQ=60)
 GTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTAT                                                                                                                                                     >  SRR19973066.196457/1‑150 (MQ=60)
    GCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTAC                                                                                                                                                  >  SRR19973066.987129/1‑150 (MQ=60)
     CCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCATAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACT                                                                                                                                                 <  SRR19973066.2553789/150‑1 (MQ=60)
     CAAATCCATGCTTCAAACAGATCGCCAATAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACT                                                                                                                                                 <  SRR19973066.2553778/150‑1 (MQ=60)
      CAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTC                                                                                                                                                >  SRR19973066.3550567/1‑150 (MQ=60)
        ATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGC                                                                                                                                              >  SRR19973066.2862363/1‑150 (MQ=60)
          CCATGCTTCAAACAGATCGCCAAGAATATACCGCGCGGCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCACAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTA                                                                                                                                            <  SRR19973066.3376416/150‑1 (MQ=60)
            ATGCTACAAACAGAACCCCAACAATATCCCGCGCGGCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCCCAAACCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAAC                                                                                                                                          <  SRR19973066.919067/150‑1 (MQ=60)
                  CAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCAC                                                                                                                                    >  SRR19973066.1371670/1‑150 (MQ=60)
                       AGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTT                                                                                                                               <  SRR19973066.3186870/150‑1 (MQ=60)
                       AGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTT                                                                                                                               <  SRR19973066.2268145/150‑1 (MQ=60)
                       AGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTT                                                                                                                               >  SRR19973066.1575388/1‑150 (MQ=60)
                        GATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTC                                                                                                                              <  SRR19973066.2012791/150‑1 (MQ=60)
                         ATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCA                                                                                                                             >  SRR19973066.2019178/1‑150 (MQ=60)
                            gcaaagtatttactcggcgagggcggtggggtcctccacggctaaaaaaccatctAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGTGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATG                                                                                                                          <  SRR19973066.1031600/95‑1 (MQ=60)
                            GCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGATAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATG                                                                                                                          <  SRR19973066.1031469/150‑1 (MQ=60)
                             CCAAGAATATCCCGCGCGTCGGCCTGGCGGGCTCCCCCCGCTAAAAAACGCACAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGA                                                                                                                         <  SRR19973066.17798/150‑1 (MQ=60)
                             CCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGA                                                                                                                         <  SRR19973066.790831/150‑1 (MQ=60)
                             CCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTAAATGA                                                                                                                         >  SRR19973066.2146575/1‑150 (MQ=60)
                               AAGAATATACCGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGANA                                                                                                                       <  SRR19973066.3088368/150‑1 (MQ=60)
                               AAGAATATACCGCGCGTCGGCCTTGCGGGCTACCCCCGCTAAAAAACGCACAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATA                                                                                                                       <  SRR19973066.14931/150‑1 (MQ=60)
                                AGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCCATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAA                                                                                                                      >  SRR19973066.61359/1‑150 (MQ=60)
                                 GAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAAC                                                                                                                     >  SRR19973066.2317892/1‑150 (MQ=60)
                                  AATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACG                                                                                                                    >  SRR19973066.2859370/1‑150 (MQ=60)
                                    TATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTC                                                                                                                  >  SRR19973066.3313212/1‑150 (MQ=60)
                                     ATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCT                                                                                                                 >  SRR19973066.1578279/1‑150 (MQ=60)
                                     ATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCT                                                                                                                 <  SRR19973066.1810128/150‑1 (MQ=60)
                                     ATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCT                                                                                                                 >  SRR19973066.1448289/1‑150 (MQ=60)
                                     ATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCT                                                                                                                 <  SRR19973066.2378545/150‑1 (MQ=60)
                                     ATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCT                                                                                                                 <  SRR19973066.2568996/150‑1 (MQ=60)
                                     ATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGATAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCT                                                                                                                 <  SRR19973066.1810132/150‑1 (MQ=60)
                                       ACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAATATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGGCTTC                                                                                                               >  SRR19973066.2107130/1‑150 (MQ=60)
                                        CAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCT                                                                                                              >  SRR19973066.1642206/1‑150 (MQ=60)
                                        CAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCT                                                                                                              <  SRR19973066.3241255/150‑1 (MQ=60)
                                        CAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCT                                                                                                              >  SRR19973066.1642201/1‑150 (MQ=60)
                                        CAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCT                                                                                                              >  SRR19973066.1640802/1‑150 (MQ=60)
                                        CAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCT                                                                                                              >  SRR19973066.1640711/1‑150 (MQ=60)
                                         agcgcggcccccccccccccttccccccaTAAAAAACGCAGAACACCGGCGGGGAGCGCCGGTTCTTCCCCGCAGAGATGAAGAACACCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTT                                                                                                             <  SRR19973066.3421168/121‑1 (MQ=60)
                                         CGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCACAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTT                                                                                                             <  SRR19973066.61853/150‑1 (MQ=60)
                                         AGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCCCGCTTTCAATGATAACGTCTTCTT                                                                                                             >  SRR19973066.1556762/1‑150 (MQ=60)
                                         AGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTT                                                                                                             <  SRR19973066.1805943/150‑1 (MQ=60)
                                           CGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTT                                                                                                           <  SRR19973066.3286242/150‑1 (MQ=60)
                                           CGCGTCGGCCTTGCGGGCTTCCCCCGATAAAAAACGCACAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTT                                                                                                           <  SRR19973066.626740/150‑1 (MQ=60)
                                              GTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCACAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGC                                                                                                        <  SRR19973066.2746302/150‑1 (MQ=60)
                                               TCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCA                                                                                                       <  SRR19973066.3522890/150‑1 (MQ=60)
                                               TCGGCCTTGCGGGCTTCCCCCGATAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAACGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCA                                                                                                       <  SRR19973066.3522894/150‑1 (MQ=60)
                                               TCGGCCTTGCGGGCTTACCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTTTAGCCACTATTACTCGCTAAAGGTCACGCTATCAATGATAACCTCTTCTTTTGGCA                                                                                                       >  SRR19973066.2743268/1‑150 (MQ=60)
                                                 GGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACG                                                                                                     >  SRR19973066.1353590/1‑150 (MQ=60)
                                                 GGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACG                                                                                                     >  SRR19973066.1340310/1‑150 (MQ=60)
                                                 GGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACCCAGAGATGTAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACG                                                                                                     >  SRR19973066.1341056/1‑150 (MQ=60)
                                                            CTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCAT                                                                                          >  SRR19973066.2090657/1‑150 (MQ=60)
                                                            CTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCAT                                                                                          >  SRR19973066.3133812/1‑150 (MQ=60)
                                                            CTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGACGGTTATTACACGCAGAGATGAAGATCTGCAATAAATAGTGTCGCCACTATAACTCGCTAAAGGTCACGCTTTCAATGATAAAGTATTCTTTTGTCACGTAATGGTGCAT                                                                                          >  SRR19973066.3133824/1‑150 (MQ=60)
                                                               CCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACC                                                                                       <  SRR19973066.183521/150‑1 (MQ=60)
                                                               CCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACC                                                                                       <  SRR19973066.183575/150‑1 (MQ=60)
                                                                   CGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTA                                                                                   >  SRR19973066.1215449/1‑150 (MQ=60)
                                                                    GCTAAAAAACGCAGAAACCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTAC                                                                                  <  SRR19973066.3212092/150‑1 (MQ=60)
                                                                                AGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAA                                                                      >  SRR19973066.2492365/1‑150 (MQ=60)
                                                                                        GGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTG                                                              <  SRR19973066.1487001/150‑1 (MQ=60)
                                                                                           GGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATT                                                           <  SRR19973066.3262177/150‑1 (MQ=60)
                                                                                            GTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTT                                                          >  SRR19973066.45482/1‑150 (MQ=60)
                                                                                             TGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGCGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTT                                                         <  SRR19973066.3385656/150‑1 (MQ=60)
                                                                                             TGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTT                                                         <  SRR19973066.3385622/150‑1 (MQ=60)
                                                                                             TGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTT                                                         <  SRR19973066.1605944/150‑1 (MQ=60)
                                                                                              GATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTG                                                        <  SRR19973066.2683490/150‑1 (MQ=60)
                                                                                                     GGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCA                                                 <  SRR19973066.1587829/150‑1 (MQ=60)
                                                                                                     GGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCA                                                 >  SRR19973066.2864818/1‑150 (MQ=60)
                                                                                                      GTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCAC                                                >  SRR19973066.3484575/1‑150 (MQ=60)
                                                                                                      GTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCAC                                                >  SRR19973066.590388/1‑150 (MQ=60)
                                                                                                       TTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACG                                               <  SRR19973066.1446691/150‑1 (MQ=60)
                                                                                                       TTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACG                                               >  SRR19973066.1204317/1‑150 (MQ=60)
                                                                                                         CTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTC                                             <  SRR19973066.697466/150‑1 (MQ=60)
                                                                                                            CCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCAT                                          >  SRR19973066.2046347/1‑150 (MQ=60)
                                                                                                               CGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCC                                       <  SRR19973066.75433/150‑1 (MQ=60)
                                                                                                                 CAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGT                                     >  SRR19973066.1333946/1‑150 (MQ=60)
                                                                                                                 CAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGT                                     >  SRR19973066.1330828/1‑150 (MQ=60)
                                                                                                                   GAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCA                                   >  SRR19973066.1337552/1‑150 (MQ=60)
                                                                                                                   GAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCA                                   <  SRR19973066.2498938/150‑1 (MQ=60)
                                                                                                                       TGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCA                               <  SRR19973066.2139444/150‑1 (MQ=60)
                                                                                                                         AAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACT                             >  SRR19973066.3059984/1‑150 (MQ=60)
                                                                                                                         AAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACT                             >  SRR19973066.3509370/1‑150 (MQ=60)
                                                                                                                         AAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACT                             >  SRR19973066.3180242/1‑150 (MQ=60)
                                                                                                                          AGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTT                            >  SRR19973066.3160851/1‑150 (MQ=60)
                                                                                                                          AGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTT                            >  SRR19973066.173015/1‑150 (MQ=60)
                                                                                                                          AGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTT                            >  SRR19973066.2114629/1‑150 (MQ=60)
                                                                                                                            ATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCA                          <  SRR19973066.1701355/150‑1 (MQ=60)
                                                                                                                            ATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCA                          <  SRR19973066.866320/150‑1 (MQ=60)
                                                                                                                            ATCTGCAATAAAGAGTGTCGCCACTATTACTAGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCA                          <  SRR19973066.2843488/150‑1 (MQ=60)
                                                                                                                               TGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCA                       <  SRR19973066.2742450/150‑1 (MQ=60)
                                                                                                                               TGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCA                       <  SRR19973066.2742456/150‑1 (MQ=60)
                                                                                                                               TGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCA                       <  SRR19973066.2742369/150‑1 (MQ=60)
                                                                                                                                    TAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACAC                  >  SRR19973066.1896940/1‑150 (MQ=60)
                                                                                                                                               GCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCC       >  SRR19973066.1535074/1‑150 (MQ=60)
                                                                                                                                                CCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCA      <  SRR19973066.3378095/150‑1 (MQ=60)
                                                                                                                                                CCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCA      <  SRR19973066.3378086/150‑1 (MQ=60)
                                                                                                                                                CCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCA      <  SRR19973066.1656122/150‑1 (MQ=60)
                                                                                                                                                 CACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAA     >  SRR19973066.1236897/1‑150 (MQ=60)
                                                                                                                                                   CTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACC   >  SRR19973066.3177605/1‑150 (MQ=60)
                                                                                                                                                    TATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCT  >  SRR19973066.2679157/1‑150 (MQ=60)
                                                                                                                                                    TATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCT  >  SRR19973066.2527313/1‑150 (MQ=60)
                                                                                                                                                    TATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCT  <  SRR19973066.2396509/150‑1 (MQ=60)
                                                                                                                                                    TATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCT  <  SRR19973066.2153982/150‑1 (MQ=60)
                                                                                                                                                    GATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCT  >  SRR19973066.1008097/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCAACCT  >  CP009273/549249‑549546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑