Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,076,526 C→A 92.8% D211Y (GAC→TAC)  speB ← agmatinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,076,5260CA92.8% 671.7 / 38.7 208D211Y (GAC→TAC) speBagmatinase
Reads supporting (aligned to +/- strand):  ref base C (9/6);  new base A (106/87);  total (115/93)
Fisher's exact test for biased strand distribution p-value = 7.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 210 total aligned reads displayed.

GCGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGT  >  CP009273/3076377‑3076672
                                                                                                                                                     |                                                                                                                                                  
gcgTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTc                                                                                                                                                    <  1:3334160/150‑1 (MQ=255)
  gTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCAt                                                                                                                                                  >  1:1356547/1‑150 (MQ=255)
   tACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATc                                                                                                                                                 >  1:3744870/1‑150 (MQ=255)
       aGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACg                                                                                                                                             <  2:4072874/150‑1 (MQ=255)
       aGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACg                                                                                                                                             <  2:535322/150‑1 (MQ=255)
        gTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGc                                                                                                                                            >  1:4341221/1‑150 (MQ=255)
            aaTAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCg                                                                                                                                        >  2:2663797/1‑150 (MQ=255)
            aaTAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCg                                                                                                                                        <  1:1655169/150‑1 (MQ=255)
               aGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATc                                                                                                                                     <  2:3687518/150‑1 (MQ=255)
               aGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATc                                                                                                                                     <  1:650152/150‑1 (MQ=255)
                   cgATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTc                                                                                                                                 >  1:3392147/1‑150 (MQ=255)
                    gATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGAAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCa                                                                                                                                >  1:2976167/1‑150 (MQ=255)
                     aTCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCAc                                                                                                                               <  1:4740068/150‑1 (MQ=255)
                      tCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCAcc                                                                                                                              >  1:425732/1‑150 (MQ=255)
                      tCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCAcc                                                                                                                              <  1:4610106/150‑1 (MQ=255)
                       cGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCt                                                                                                                             >  1:4280921/1‑150 (MQ=255)
                       cGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCt                                                                                                                             <  2:3283554/150‑1 (MQ=255)
                        ggAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACTTAATCCACGCTGCGATCGTTCACCTg                                                                                                                            >  2:2147575/1‑150 (MQ=255)
                         gAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTgg                                                                                                                           >  2:2252757/1‑150 (MQ=255)
                         gAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTgg                                                                                                                           >  1:619941/1‑150 (MQ=255)
                           ggTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCTCAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCa                                                                                                                         <  2:3937820/150‑1 (MQ=255)
                            gTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAg                                                                                                                        >  1:4445607/1‑150 (MQ=255)
                              cAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGc                                                                                                                      <  1:5011950/150‑1 (MQ=255)
                               aGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCg                                                                                                                     >  2:1519383/1‑150 (MQ=255)
                                ggCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGt                                                                                                                    >  2:890601/1‑150 (MQ=255)
                                  ccgccAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTcc                                                                                                                  <  2:3301689/150‑1 (MQ=255)
                                     ccAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGc                                                                                                               >  1:2738685/1‑150 (MQ=255)
                                          cACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCATCTGGCAGGCGTCCAGCACGGt                                                                                                          >  2:3664931/1‑150 (MQ=255)
                                           aCTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTa                                                                                                         <  1:1254342/150‑1 (MQ=255)
                                            cTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTaa                                                                                                        <  2:5040292/150‑1 (MQ=255)
                                              ggCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAg                                                                                                      <  2:1216019/150‑1 (MQ=255)
                                               gCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGc                                                                                                     <  1:2846650/150‑1 (MQ=255)
                                               gCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGc                                                                                                     <  1:1442695/150‑1 (MQ=255)
                                                cGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGcc                                                                                                    <  2:2500776/150‑1 (MQ=255)
                                                cGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGcc                                                                                                    <  2:3900867/150‑1 (MQ=255)
                                                 gTACCGGTGCCTGGTGCAACAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCg                                                                                                   <  2:3116915/150‑1 (MQ=255)
                                                   aCCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGtt                                                                                                 <  2:818248/150‑1 (MQ=255)
                                                      ggTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTc                                                                                              <  2:4632802/150‑1 (MQ=255)
                                                           cTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACACTCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTAt                                                                                         <  1:3096457/150‑1 (MQ=255)
                                                            tGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTCAAGCCGTTGTCTTTATc                                                                                        >  2:4940893/1‑150 (MQ=255)
                                                            tGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATc                                                                                        >  2:4268857/1‑150 (MQ=255)
                                                            tGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATc                                                                                        <  1:4698477/150‑1 (MQ=255)
                                                                gCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAAc                                                                                    <  1:3651355/150‑1 (MQ=255)
                                                                 cAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACt                                                                                   <  1:2075108/150‑1 (MQ=255)
                                                                  aaaaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTc                                                                                  >  1:2298342/1‑150 (MQ=255)
                                                                   aaaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCg                                                                                 >  1:2019263/1‑150 (MQ=255)
                                                                    aaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCgg                                                                                >  2:3045047/1‑150 (MQ=255)
                                                                     aGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGt                                                                               <  1:4940540/150‑1 (MQ=255)
                                                                      gCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCTTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTa                                                                              <  1:1780467/150‑1 (MQ=255)
                                                                          gATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGaa                                                                          >  2:1710959/1‑150 (MQ=255)
                                                                           aTCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAAt                                                                         >  2:3640144/1‑150 (MQ=255)
                                                                            tCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATa                                                                        >  1:3905803/1‑150 (MQ=255)
                                                                                 gCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAAt                                                                   >  1:3437297/1‑150 (MQ=255)
                                                                                 gCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTCCGAATACCAAt                                                                   >  2:2830946/1‑150 (MQ=255)
                                                                                 gCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAAt                                                                   >  2:900431/1‑150 (MQ=255)
                                                                                  cAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATc                                                                  <  2:3984946/150‑1 (MQ=255)
                                                                                   aGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCt                                                                 >  1:4012146/1‑150 (MQ=255)
                                                                                   aGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCt                                                                 >  2:3409004/1‑150 (MQ=255)
                                                                                   aGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCt                                                                 >  2:3698649/1‑150 (MQ=255)
                                                                                   aGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCt                                                                 >  1:959943/1‑150 (MQ=255)
                                                                                    gTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTg                                                                >  1:2551157/1‑150 (MQ=255)
                                                                                     tCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGc                                                               >  1:2636498/1‑150 (MQ=255)
                                                                                      cGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCa                                                              >  1:2380229/1‑150 (MQ=255)
                                                                                      cGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCa                                                              >  2:2121252/1‑150 (MQ=255)
                                                                                        atatCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACg                                                            >  1:1012806/1‑150 (MQ=255)
                                                                                             aaaaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATg                                                       >  2:2219573/1‑150 (MQ=255)
                                                                                              aaaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGa                                                      >  2:4133288/1‑150 (MQ=255)
                                                                                                aGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAtt                                                    >  2:929634/1‑150 (MQ=255)
                                                                                                 gTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTc                                                   >  1:495306/1‑150 (MQ=255)
                                                                                                 gTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTc                                                   <  1:5146198/150‑1 (MQ=255)
                                                                                                     ggTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGt                                               >  2:2729552/1‑150 (MQ=255)
                                                                                                     ggTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGt                                               >  1:837730/1‑150 (MQ=255)
                                                                                                         aaCCGGCATATCACCCACACTCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCTGTACGAATACCAATCTGCACGGAATGATTCGGGTCGAt                                           <  2:984750/150‑1 (MQ=255)
                                                                                                           ccGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCa                                         >  2:702791/1‑150 (MQ=255)
                                                                                                            cGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAg                                        <  1:1848178/150‑1 (MQ=255)
                                                                                                                atatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACct                                    >  2:4454260/1‑150 (MQ=255)
                                                                                                                atatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACct                                    >  1:4000456/1‑150 (MQ=255)
                                                                                                                atatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACct                                    >  1:2367732/1‑150 (MQ=255)
                                                                                                                atatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTGTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACct                                    >  2:2620953/1‑150 (MQ=255)
                                                                                                                   tCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACcttct                                 >  1:1027133/1‑150 (MQ=255)
                                                                                                                     aCCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCttt                               <  2:234613/150‑1 (MQ=255)
                                                                                                                      cccACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTc                              <  2:4029360/150‑1 (MQ=255)
                                                                                                                      cccACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTc                              <  2:2202218/150‑1 (MQ=255)
                                                                                                                      cccACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTc                              >  1:3157120/1‑150 (MQ=255)
                                                                                                                      cccACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTc                              <  2:4815517/150‑1 (MQ=255)
                                                                                                                       ccACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCg                             >  2:4034375/1‑150 (MQ=255)
                                                                                                                              cTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGt                      >  1:4251200/1‑150 (MQ=255)
                                                                                                                              cTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGt                      <  1:3766331/150‑1 (MQ=255)
                                                                                                                                 tttCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGtata                   >  2:4613476/1‑150 (MQ=255)
                                                                                                                                 tttCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGtata                   <  1:218617/150‑1 (MQ=255)
                                                                                                                                    cACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGaa                <  2:1411108/150‑1 (MQ=255)
                                                                                                                                       ttGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACAt             >  1:730001/1‑150 (MQ=255)
                                                                                                                                       ttGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACAt             >  2:1591270/1‑150 (MQ=255)
                                                                                                                                        tGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATa            >  2:5154037/1‑150 (MQ=255)
                                                                                                                                         gggCGATAACGTAATCCACGCTGCGATCGTTCATCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAg           <  1:3664942/150‑1 (MQ=255)
                                                                                                                                         gggCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAg           <  2:620890/150‑1 (MQ=255)
                                                                                                                                               tAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGt     <  1:1519383/150‑1 (MQ=255)
                                                                                                                                                aaCGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGACTGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTg    >  2:4697432/1‑150 (MQ=255)
                                                                                                                                                aaCGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTATATCAAACTCGGTACGAATACCAATCTGCACGGAATGATACGGGTCGATCAGACCTTCTTTCGGCGCGGTCTAGAACATAGTGCCGTg    >  2:4696221/1‑150 (MQ=255)
                                                                                                                                                  cGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGt  <  2:495306/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGT  >  CP009273/3076377‑3076672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAA  >  CP009273/3076377‑3076675
                                                                                                                                                     |                                                                                                                                                     
GCGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTC                                                                                                                                                       <  SRR19973083.3779847/150‑1 (MQ=60)
GCGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTC                                                                                                                                                       <  SRR19973083.3349747/150‑1 (MQ=60)
  GTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCAT                                                                                                                                                     >  SRR19973083.1363227/1‑150 (MQ=60)
  GTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAAT                                                                                                                                                     <  SRR19973083.4566945/150‑1 (MQ=60)
  GTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAAT                                                                                                                                                     >  SRR19973083.4711276/1‑150 (MQ=60)
   TACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATC                                                                                                                                                    >  SRR19973083.3761850/1‑150 (MQ=60)
   TACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATC                                                                                                                                                    >  SRR19973083.4284678/1‑150 (MQ=60)
        GTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGC                                                                                                                                               >  SRR19973083.4360041/1‑150 (MQ=60)
           TAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGC                                                                                                                                            >  SRR19973083.235884/1‑150 (MQ=60)
            AATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCG                                                                                                                                           <  SRR19973083.1663383/150‑1 (MQ=60)
             ATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGA                                                                                                                                          <  SRR19973083.1797102/150‑1 (MQ=60)
               AGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATC                                                                                                                                        <  SRR19973083.653417/150‑1 (MQ=60)
                   CGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTC                                                                                                                                    >  SRR19973083.3407850/1‑150 (MQ=60)
                    GATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGAAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCA                                                                                                                                   >  SRR19973083.2990602/1‑150 (MQ=60)
                     ATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCAC                                                                                                                                  >  SRR19973083.4324776/1‑150 (MQ=60)
                     ATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCAC                                                                                                                                  <  SRR19973083.4760307/150‑1 (MQ=60)
                      TCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACC                                                                                                                                 <  SRR19973083.4630013/150‑1 (MQ=60)
                      TCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACC                                                                                                                                 >  SRR19973083.427632/1‑150 (MQ=60)
                      TCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACC                                                                                                                                 <  SRR19973083.1283755/150‑1 (MQ=60)
                      TCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACC                                                                                                                                 <  SRR19973083.3890856/150‑1 (MQ=60)
                       CGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCT                                                                                                                                >  SRR19973083.4299576/1‑150 (MQ=60)
                         GAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGG                                                                                                                              >  SRR19973083.624002/1‑150 (MQ=60)
                         GAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGG                                                                                                                              >  SRR19973083.623049/1‑150 (MQ=60)
                           GGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCA                                                                                                                            <  SRR19973083.78703/150‑1 (MQ=60)
                           GGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCA                                                                                                                            <  SRR19973083.3497128/150‑1 (MQ=60)
                            GTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAG                                                                                                                           >  SRR19973083.4464937/1‑150 (MQ=60)
                            GTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAG                                                                                                                           <  SRR19973083.4367109/150‑1 (MQ=60)
                              CAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGC                                                                                                                         <  SRR19973083.5033450/150‑1 (MQ=60)
                              CAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGC                                                                                                                         <  SRR19973083.342405/150‑1 (MQ=60)
                                     CCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGC                                                                                                                  >  SRR19973083.2752347/1‑150 (MQ=60)
                                        ATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACG                                                                                                               <  SRR19973083.4735080/150‑1 (MQ=60)
                                           ACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTA                                                                                                            <  SRR19973083.1260461/150‑1 (MQ=60)
                                            CTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAA                                                                                                           <  SRR19973083.3106144/150‑1 (MQ=60)
                                              GGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAG                                                                                                         >  SRR19973083.257440/1‑150 (MQ=60)
                                               GCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGC                                                                                                        <  SRR19973083.1449947/150‑1 (MQ=60)
                                               GCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGC                                                                                                        <  SRR19973083.2860711/150‑1 (MQ=60)
                                                      GGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTC                                                                                                 <  SRR19973083.4985366/150‑1 (MQ=60)
                                                      GGTGCCTGGTGCAAAAGCAGGAGCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTC                                                                                                 <  SRR19973083.3402593/150‑1 (MQ=60)
                                                           CTGGTGCAAATGCAGGATCCAGGCAGTCGATATCAATAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGTTAAAGCCGTTGTCTTTAT                                                                                            <  SRR19973083.4007002/150‑1 (MQ=60)
                                                           CTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACACTCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTAT                                                                                            <  SRR19973083.3111296/150‑1 (MQ=60)
                                                           CTGGTGCAAAAGCAGGATCCAGGCAGTCGATATAAAAAGTCAGGTAAACCGGCATATCACACACAATCTGTTTCACTTGTGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTAT                                                                                            <  SRR19973083.1444781/150‑1 (MQ=60)
                                                            TGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATC                                                                                           <  SRR19973083.4718615/150‑1 (MQ=60)
                                                            TGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATC                                                                                           >  SRR19973083.2888032/1‑150 (MQ=60)
                                                                GCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAAC                                                                                       <  SRR19973083.3668054/150‑1 (MQ=60)
                                                                GCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAAC                                                                                       <  SRR19973083.3203587/150‑1 (MQ=60)
                                                                 CAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACT                                                                                      <  SRR19973083.2085656/150‑1 (MQ=60)
                                                                  AAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTC                                                                                     >  SRR19973083.2310113/1‑150 (MQ=60)
                                                                   AAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCG                                                                                    >  SRR19973083.2029576/1‑150 (MQ=60)
                                                                     AGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGT                                                                                  <  SRR19973083.4961779/150‑1 (MQ=60)
                                                                      GCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCTTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTA                                                                                 <  SRR19973083.1789352/150‑1 (MQ=60)
                                                                            TCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATA                                                                           >  SRR19973083.3923198/1‑150 (MQ=60)
                                                                            TCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATA                                                                           >  SRR19973083.3923186/1‑150 (MQ=60)
                                                                              CAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACC                                                                         <  SRR19973083.1987057/150‑1 (MQ=60)
                                                                                 GCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAAT                                                                      >  SRR19973083.3453101/1‑150 (MQ=60)
                                                                                   AGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCT                                                                    >  SRR19973083.4029959/1‑150 (MQ=60)
                                                                                   AGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCT                                                                    >  SRR19973083.4029992/1‑150 (MQ=60)
                                                                                   AGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCT                                                                    >  SRR19973083.964687/1‑150 (MQ=60)
                                                                                    GTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTG                                                                   >  SRR19973083.2564064/1‑150 (MQ=60)
                                                                                     TCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGC                                                                  >  SRR19973083.2649742/1‑150 (MQ=60)
                                                                                      CGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCA                                                                 <  SRR19973083.2201214/150‑1 (MQ=60)
                                                                                      CGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCA                                                                 >  SRR19973083.2392436/1‑150 (MQ=60)
                                                                                      CGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCA                                                                 >  SRR19973083.2392475/1‑150 (MQ=60)
                                                                                        ATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACG                                                               >  SRR19973083.1017876/1‑150 (MQ=60)
                                                                                        ATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACG                                                               >  SRR19973083.3130290/1‑150 (MQ=60)
                                                                                        ATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACG                                                               >  SRR19973083.1017900/1‑150 (MQ=60)
                                                                                            CAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAAT                                                           <  SRR19973083.2753879/150‑1 (MQ=60)
                                                                                                 GTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTC                                                      >  SRR19973083.497655/1‑150 (MQ=60)
                                                                                                 GTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTC                                                      >  SRR19973083.497692/1‑150 (MQ=60)
                                                                                                 GTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTC                                                      <  SRR19973083.5168063/150‑1 (MQ=60)
                                                                                                 GTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTC                                                      <  SRR19973083.2509207/150‑1 (MQ=60)
                                                                                                    AGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGG                                                   >  SRR19973083.2106591/1‑150 (MQ=60)
                                                                                                     GGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGT                                                  >  SRR19973083.3761068/1‑150 (MQ=60)
                                                                                                     GGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGT                                                  >  SRR19973083.841693/1‑150 (MQ=60)
                                                                                                      GTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTC                                                 <  SRR19973083.4168289/150‑1 (MQ=60)
                                                                                                        AAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGA                                               <  SRR19973083.2072179/150‑1 (MQ=60)
                                                                                                            CGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAG                                           <  SRR19973083.1857500/150‑1 (MQ=60)
                                                                                                               CATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATACGGGTCGAGCAGACC                                        >  SRR19973083.708913/1‑150 (MQ=60)
                                                                                                                ATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCT                                       >  SRR19973083.4018246/1‑150 (MQ=60)
                                                                                                                ATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCT                                       >  SRR19973083.2379920/1‑150 (MQ=60)
                                                                                                                   TCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCT                                    >  SRR19973083.1032282/1‑150 (MQ=60)
                                                                                                                      CCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTC                                 >  SRR19973083.3172186/1‑150 (MQ=60)
                                                                                                                      CCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTC                                 >  SRR19973083.5158201/1‑150 (MQ=60)
                                                                                                                      CCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTC                                 <  SRR19973083.1744004/150‑1 (MQ=60)
                                                                                                                              CTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGT                         >  SRR19973083.4270468/1‑150 (MQ=60)
                                                                                                                              CTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGT                         >  SRR19973083.4269780/1‑150 (MQ=60)
                                                                                                                              CTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGT                         <  SRR19973083.3783366/150‑1 (MQ=60)
                                                                                                                                 TTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATA                      >  SRR19973083.1618761/1‑150 (MQ=60)
                                                                                                                                 TTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATA                      <  SRR19973083.1117748/150‑1 (MQ=60)
                                                                                                                                 TTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATA                      <  SRR19973083.219814/150‑1 (MQ=60)
                                                                                                                                  TTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAG                     >  SRR19973083.4954660/1‑150 (MQ=60)
                                                                                                                                   TCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGA                    >  SRR19973083.2066078/1‑150 (MQ=60)
                                                                                                                                    CACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAA                   >  SRR19973083.4929196/1‑150 (MQ=60)
                                                                                                                                       TTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACAT                >  SRR19973083.5177376/1‑150 (MQ=60)
                                                                                                                                       TTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACAT                >  SRR19973083.733600/1‑150 (MQ=60)
                                                                                                                                        TGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATA               <  SRR19973083.3058765/150‑1 (MQ=60)
                                                                                                                                         GGGCGATAACGTAATCCACGCTGCGATCGTTCATCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAG              <  SRR19973083.3681679/150‑1 (MQ=60)
                                                                                                                                         GGGCGATAACGTAATCCACGCTGCGATCGTTCATCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAG              <  SRR19973083.3681668/150‑1 (MQ=60)
                                                                                                                                           GCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTG            <  SRR19973083.88109/150‑1 (MQ=60)
                                                                                                                                             GATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCC          >  SRR19973083.2673676/1‑150 (MQ=60)
                                                                                                                                               TAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGT        <  SRR19973083.1526991/150‑1 (MQ=60)
                                                                                                                                               TAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGT        <  SRR19973083.1527026/150‑1 (MQ=60)
                                                                                                                                                   GTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTC    >  SRR19973083.5070915/1‑150 (MQ=60)
                                                                                                                                                     AATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAA  <  SRR19973083.5068747/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GCGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTCAA  >  CP009273/3076377‑3076675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑