Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F247 I0 R1	1496	299.8	9582018	97.8%	9371213	149.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,076,526	C→A	100%	D211Y (GAC→TAC)	speB ←	agmatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,076,526	0	C	A	99.6%	870.9 / ‑4.1	251	D211Y (GAC→TAC)	speB	agmatinase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (121/129); total (121/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 251 total aligned reads displayed.

TACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTG  >  CP009273/3076380‑3076670

  ccAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATcc                                                                                                                                              <  1:949751/149‑1 (MQ=255)

  ccAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATcc                                                                                                                                              <  1:2044541/149‑1 (MQ=255)

  ccAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATcc                                                                                                                                              >  2:192226/1‑149 (MQ=255)

   cAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCa                                                                                                                                             <  2:3631680/149‑1 (MQ=255)

      tttAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGc                                                                                                                                          >  2:2220356/1‑149 (MQ=255)

         aaTAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGc                                                                                                                                       <  2:3036415/149‑1 (MQ=255)

         aaTAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGc                                                                                                                                       <  2:1602488/149‑1 (MQ=255)

            aGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGAt                                                                                                                                    <  1:1605698/149‑1 (MQ=255)

               gcgATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAGAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGt                                                                                                                                 >  2:1034094/1‑149 (MQ=255)

               gcgATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGt                                                                                                                                 <  1:3051604/149‑1 (MQ=255)

               gcgATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGt                                                                                                                                 <  2:4078644/149‑1 (MQ=255)

               gcgATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGAGATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCgg                                                                                                                                 >  2:3891070/1‑148 (MQ=255)

                 gATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTc                                                                                                                               <  1:2320543/149‑1 (MQ=255)

                  ataggAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCa                                                                                                                              <  1:4348122/146‑1 (MQ=255)

                  aTCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCa                                                                                                                              <  1:3456374/149‑1 (MQ=255)

                  aTCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCa                                                                                                                              <  1:2450971/149‑1 (MQ=255)

                     ggAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCt                                                                                                                           >  2:4344596/1‑149 (MQ=255)

                      gAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTg                                                                                                                          <  2:1437179/149‑1 (MQ=255)

                       aGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTgg                                                                                                                         <  1:2517862/149‑1 (MQ=255)

                          tCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAg                                                                                                                      <  2:544560/149‑1 (MQ=255)

                          tCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAg                                                                                                                      <  2:3503129/149‑1 (MQ=255)

                             ggCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCg                                                                                                                   >  2:2021811/1‑149 (MQ=255)

                             ggCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCg                                                                                                                   >  1:180299/1‑149 (MQ=255)

                             ggCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCg                                                                                                                   >  1:376859/1‑149 (MQ=255)

                               ccgccAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTc                                                                                                                 <  1:4292024/149‑1 (MQ=255)

                                cgccAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTcc                                                                                                                <  1:99572/149‑1 (MQ=255)

                                cgccAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTcc                                                                                                                >  2:4474973/1‑149 (MQ=255)

                                 gccAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCa                                                                                                               >  1:3109863/1‑149 (MQ=255)

                                             cGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGc                                                                                                   <  1:1687842/149‑1 (MQ=255)

                                             cGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGc                                                                                                   <  2:2808796/149‑1 (MQ=255)

                                                aCCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGt                                                                                                <  2:3809643/149‑1 (MQ=255)

                                                 ccGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGtt                                                                                               >  2:2133281/1‑149 (MQ=255)

                                                    gTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTc                                                                                            <  2:2718040/149‑1 (MQ=255)

                                                     tGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCt                                                                                           <  1:3285400/149‑1 (MQ=255)

                                                     tGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCt                                                                                           <  1:4483326/149‑1 (MQ=255)

                                                      gCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCtt                                                                                          >  1:199935/1‑149 (MQ=255)

                                                          ggTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATc                                                                                      <  1:1233290/149‑1 (MQ=255)

                                                          ggTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATc                                                                                      >  2:2498336/1‑149 (MQ=255)

                                                          ggTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATc                                                                                      <  1:4274080/149‑1 (MQ=255)

                                                          ggTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATc                                                                                      <  1:1233370/149‑1 (MQ=255)

                                                              cAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAAc                                                                                  <  1:2700797/149‑1 (MQ=255)

                                                              cAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAAc                                                                                  <  1:856188/149‑1 (MQ=255)

                                                               aaaaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACt                                                                                 >  1:1596655/1‑149 (MQ=255)

                                                                aaaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTc                                                                                >  2:369688/1‑149 (MQ=255)

                                                                 aaGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCg                                                                               >  1:1376298/1‑149 (MQ=255)

                                                                   gCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGt                                                                             <  1:702946/149‑1 (MQ=255)

                                                                    cAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTa                                                                            <  2:4759617/149‑1 (MQ=255)

                                                                    cAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTa                                                                            <  1:1156154/149‑1 (MQ=255)

                                                                    cAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTa                                                                            <  2:2362451/149‑1 (MQ=255)

                                                                     aGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTAc                                                                           >  2:4397043/1‑149 (MQ=255)

                                                                      ggATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACg                                                                          >  2:4183585/1‑149 (MQ=255)

                                                                        aTCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGaa                                                                        >  1:835515/1‑149 (MQ=255)

                                                                        aTCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGaa                                                                        >  2:3289645/1‑149 (MQ=255)

                                                                         tCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAAt                                                                       <  1:2400636/149‑1 (MQ=255)

                                                                         tCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAAt                                                                       >  1:1911919/1‑149 (MQ=255)

                                                                         tCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAAt                                                                       <  1:4045910/149‑1 (MQ=255)

                                                                             ggCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCa                                                                   >  2:515343/1‑149 (MQ=255)

                                                                               cAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAAt                                                                 <  1:1642199/149‑1 (MQ=255)

                                                                                aGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATc                                                                >  1:4470844/1‑149 (MQ=255)

                                                                                aGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATc                                                                <  1:1995375/149‑1 (MQ=255)

                                                                                 gTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCt                                                               >  1:1372514/1‑149 (MQ=255)

                                                                                   cGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGc                                                             <  2:3514842/149‑1 (MQ=255)

                                                                                     atatCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCAc                                                           >  2:1822476/1‑149 (MQ=255)

                                                                                        tCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTATTCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAAACTGCACGGa                                                        <  2:1982888/149‑1 (MQ=255)

                                                                                          aaaaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAAt                                                      <  2:1502773/149‑1 (MQ=255)

                                                                                          aaaaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAAt                                                      >  2:796414/1‑149 (MQ=255)

                                                                                            aaGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGa                                                    >  1:593182/1‑149 (MQ=255)

                                                                                              gTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAtt                                                  >  2:174675/1‑149 (MQ=255)

                                                                                               tCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTc                                                 >  1:3157344/1‑149 (MQ=255)

                                                                                                 aGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCgg                                               >  1:325715/1‑149 (MQ=255)

                                                                                                   gTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGt                                             <  2:2527948/149‑1 (MQ=255)

                                                                                                    tAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTc                                            >  1:578942/1‑149 (MQ=255)

                                                                                                    tAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTc                                            <  2:3406500/149‑1 (MQ=255)

                                                                                                    tAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTc                                            <  2:3406172/149‑1 (MQ=255)

                                                                                                       aCCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGAt                                         <  2:1096737/149‑1 (MQ=255)

                                                                                                        ccGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATc                                        <  2:536801/149‑1 (MQ=255)

                                                                                                          ggCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAg                                      >  1:2966706/1‑149 (MQ=255)

                                                                                                          ggCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAg                                      >  1:3964181/1‑149 (MQ=255)

                                                                                                              tatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACct                                  >  1:1427669/1‑149 (MQ=255)

                                                                                                              tatCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTAGGGTCGATCAGAcat                                  >  2:3932425/1‑147 (MQ=255)

                                                                                                                tCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACcttc                                >  1:4631665/1‑149 (MQ=255)

                                                                                                                tCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACcttc                                >  2:723920/1‑149 (MQ=255)

                                                                                                                   cccACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCttt                             <  1:3947839/149‑1 (MQ=255)

                                                                                                                   cccACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCttt                             <  1:1558585/149‑1 (MQ=255)

                                                                                                                     cacaATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCg                           >  2:1295256/1‑149 (MQ=255)

                                                                                                                     cacaATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCg                           >  2:2286286/1‑149 (MQ=255)

                                                                                                                      acaATCTGTTTCACTTGGGCGATAACGTACTCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGACTGATTCGGGTCGATCAGACCTTCTTTCgg                          >  1:264207/1‑149 (MQ=255)

                                                                                                                       caATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGgc                         <  2:3920157/149‑1 (MQ=255)

                                                                                                                       caATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGgc                         >  2:1254874/1‑149 (MQ=255)

                                                                                                                       caATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGgc                         >  2:1479446/1‑149 (MQ=255)

                                                                                                                            tGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGt                    >  1:1628291/1‑149 (MQ=255)

                                                                                                                              tttCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGtct                  >  1:4142983/1‑147 (MQ=255)

                                                                                                                                   cTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAAc             >  1:427682/1‑149 (MQ=255)

                                                                                                                                    ttGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACa            <  2:2905391/149‑1 (MQ=255)

                                                                                                                                     tGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACAt           <  2:4627660/149‑1 (MQ=255)

                                                                                                                                      gggCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATa          <  1:1608193/149‑1 (MQ=255)

                                                                                                                                            tAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCg    >  2:889796/1‑149 (MQ=255)

                                                                                                                                             aaCGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGt   >  2:3186706/1‑149 (MQ=255)

                                                                                                                                              aCGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTCTAGAACATAGTGCCGTg  >  2:4406399/1‑149 (MQ=255)

                                                                                                                                              aCGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTg  >  2:4280910/1‑149 (MQ=255)

TACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTG  >  CP009273/3076380‑3076670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTC  >  CP009273/3076377‑3076673

GCGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTA                                                                                                                                                     <  SRR19973071.978531/150‑1 (MQ=60)

GCGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTA                                                                                                                                                     <  SRR19973071.978103/150‑1 (MQ=60)

 CGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAA                                                                                                                                                    <  SRR19973071.2878742/150‑1 (MQ=60)

 CGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAA                                                                                                                                                    <  SRR19973071.2878795/150‑1 (MQ=60)

 CGTACCAGTTTAATAGAGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAA                                                                                                                                                    >  SRR19973071.504875/1‑150 (MQ=60)

 CGTACCAGTTTAATAGAGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAA                                                                                                                                                    >  SRR19973071.504835/1‑150 (MQ=60)

  GTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAAT                                                                                                                                                   <  SRR19973071.2033705/150‑1 (MQ=60)

  GTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGAAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTGTCACTTGGGCGATAACGTAAT                                                                                                                                                   >  SRR19973071.1673498/1‑150 (MQ=60)

     CCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCA                                                                                                                                                <  SRR19973071.2052037/150‑1 (MQ=60)

     CCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCA                                                                                                                                                <  SRR19973071.953195/150‑1 (MQ=60)

     CCAGTGTACTAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCCGGCAGTCGATATCAACAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCA                                                                                                                                                <  SRR19973071.2050872/150‑1 (MQ=60)

         TTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCT                                                                                                                                            <  SRR19973071.259923/150‑1 (MQ=60)

             ATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGA                                                                                                                                        <  SRR19973071.296189/150‑1 (MQ=60)

              TAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGAT                                                                                                                                       <  SRR19973071.3974572/150‑1 (MQ=60)

              TAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGAT                                                                                                                                       >  SRR19973071.4458725/1‑150 (MQ=60)

               AGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATC                                                                                                                                      <  SRR19973071.1611341/150‑1 (MQ=60)

               AGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATC                                                                                                                                      >  SRR19973071.3084059/1‑150 (MQ=60)

               AGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATC                                                                                                                                      <  SRR19973071.1611359/150‑1 (MQ=60)

                  GCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTT                                                                                                                                   <  SRR19973071.3062439/150‑1 (MQ=60)

                  GCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTT                                                                                                                                   <  SRR19973071.3905135/150‑1 (MQ=60)

                    GATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCA                                                                                                                                 <  SRR19973071.2328994/150‑1 (MQ=60)

                    GATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCA                                                                                                                                 <  SRR19973071.1801535/150‑1 (MQ=60)

                     ATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGTCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCAC                                                                                                                                <  SRR19973071.2459937/150‑1 (MQ=60)

                     ATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCAC                                                                                                                                <  SRR19973071.3468574/150‑1 (MQ=60)

                     ATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCAC                                                                                                                                <  SRR19973071.2459876/150‑1 (MQ=60)

                     ATAGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCAC                                                                                                                                <  SRR19973071.4363447/150‑1 (MQ=60)

                        GGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTG                                                                                                                             >  SRR19973071.738151/1‑150 (MQ=60)

                          AGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCNGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGC                                                                                                                           <  SRR19973071.2345993/150‑1 (MQ=60)

                          AGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGC                                                                                                                           <  SRR19973071.2527028/150‑1 (MQ=60)

                          AGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGC                                                                                                                           <  SRR19973071.2527032/150‑1 (MQ=60)

                               AGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCG                                                                                                                      >  SRR19973071.378048/1‑150 (MQ=60)

                               AGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCG                                                                                                                      >  SRR19973071.181055/1‑150 (MQ=60)

                                  CCGCCAATCACTGTCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCC                                                                                                                   <  SRR19973071.637931/150‑1 (MQ=60)

                                  CCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCC                                                                                                                   <  SRR19973071.4306631/150‑1 (MQ=60)

                                  CCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCC                                                                                                                   <  SRR19973071.4307216/150‑1 (MQ=60)

                                   CGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCA                                                                                                                  <  SRR19973071.100089/150‑1 (MQ=60)

                                   CGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCA                                                                                                                  >  SRR19973071.3120813/1‑150 (MQ=60)

                                   CGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCA                                                                                                                  <  SRR19973071.100117/150‑1 (MQ=60)

                                      CAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCA                                                                                                               >  SRR19973071.2101815/1‑150 (MQ=60)

                                        ATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACG                                                                                                             <  SRR19973071.4287696/150‑1 (MQ=60)

                                               GCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGC                                                                                                      <  SRR19973071.751407/150‑1 (MQ=60)

                                                CGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCC                                                                                                     <  SRR19973071.1694069/150‑1 (MQ=60)

                                                    CCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTG                                                                                                 >  SRR19973071.4454289/1‑150 (MQ=60)

                                                     CGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGT                                                                                                >  SRR19973071.3023897/1‑150 (MQ=60)

                                                        TGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTT                                                                                             >  SRR19973071.200752/1‑150 (MQ=60)

                                                        TGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTT                                                                                             <  SRR19973071.3297040/150‑1 (MQ=60)

                                                        TGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTT                                                                                             <  SRR19973071.4499240/150‑1 (MQ=60)

                                                         GCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTT                                                                                            <  SRR19973071.1692169/150‑1 (MQ=60)

                                                          CCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTA                                                                                           >  SRR19973071.3295657/1‑150 (MQ=60)

                                                           CTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTAT                                                                                          <  SRR19973071.3212323/150‑1 (MQ=60)

                                                           CTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTAT                                                                                          <  SRR19973071.3211911/150‑1 (MQ=60)

                                                             GGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCA                                                                                        <  SRR19973071.4289224/150‑1 (MQ=60)

                                                             GGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCA                                                                                        <  SRR19973071.1237646/150‑1 (MQ=60)

                                                             GGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCA                                                                                        <  SRR19973071.1237715/150‑1 (MQ=60)

                                                             GGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCA                                                                                        <  SRR19973071.1237726/150‑1 (MQ=60)

                                                             GGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGGAAACCGGCATATCACCCACAAGCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTGATCA                                                                                        <  SRR19973071.1237692/150‑1 (MQ=60)

                                                                 CAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACT                                                                                    >  SRR19973071.1602294/1‑150 (MQ=60)

                                                                 CAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACT                                                                                    >  SRR19973071.1602288/1‑150 (MQ=60)

                                                                 CAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACT                                                                                    <  SRR19973071.2710436/150‑1 (MQ=60)

                                                                 CAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACT                                                                                    <  SRR19973071.3605969/150‑1 (MQ=60)

                                                                 CAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACT                                                                                    <  SRR19973071.859239/150‑1 (MQ=60)

                                                                   AAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCG                                                                                  >  SRR19973071.1381378/1‑150 (MQ=60)

                                                                     AGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGT                                                                                >  SRR19973071.2435296/1‑150 (MQ=60)

                                                                     AGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGT                                                                                >  SRR19973071.2877566/1‑150 (MQ=60)

                                                                     AGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGT                                                                                >  SRR19973071.2435263/1‑150 (MQ=60)

                                                                      GCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTA                                                                               <  SRR19973071.705420/150‑1 (MQ=60)

                                                                       CAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTAC                                                                              <  SRR19973071.1160165/150‑1 (MQ=60)

                                                                          GATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAA                                                                           >  SRR19973071.838452/1‑150 (MQ=60)

                                                                           ATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAAT                                                                          >  SRR19973071.1918925/1‑150 (MQ=60)

                                                                            TCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATA                                                                         <  SRR19973071.4060105/150‑1 (MQ=60)

                                                                            TCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATA                                                                         <  SRR19973071.2409278/150‑1 (MQ=60)

                                                                            TCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATA                                                                         <  SRR19973071.4060196/150‑1 (MQ=60)

                                                                                  CAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATC                                                                   >  SRR19973071.4486694/1‑150 (MQ=60)

                                                                                  CAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATC                                                                   <  SRR19973071.1648169/150‑1 (MQ=60)

                                                                                  CAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATC                                                                   >  SRR19973071.4486699/1‑150 (MQ=60)

                                                                                   AGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCT                                                                  >  SRR19973071.3122127/1‑150 (MQ=60)

                                                                                   AGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCT                                                                  <  SRR19973071.2002570/150‑1 (MQ=60)

                                                                                   AGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCT                                                                  <  SRR19973071.2002752/150‑1 (MQ=60)

                                                                                   AGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCT                                                                  <  SRR19973071.2002574/150‑1 (MQ=60)

                                                                                   AGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCT                                                                  >  SRR19973071.1377581/1‑150 (MQ=60)

                                                                                    GTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTG                                                                 >  SRR19973071.331792/1‑150 (MQ=60)

                                                                                    GTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTG                                                                 <  SRR19973071.19661/150‑1 (MQ=60)

                                                                                      CGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCA                                                               <  SRR19973071.853684/150‑1 (MQ=60)

                                                                                      CGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCA                                                               <  SRR19973071.4033780/150‑1 (MQ=60)

                                                                                       GATATCAAAAGTAAGGTAAACCGGCATATAACCCACAATATGTTTCACTTGGGCGATAACGAAATCCACGCTGCGATCTTTCACCTGGCAGGCGACCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCAC                                                              >  SRR19973071.3823232/1‑150 (MQ=60)

                                                                                         TATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGG                                                            >  SRR19973071.1878141/1‑150 (MQ=60)

                                                                                            CAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAATCTCGGTACGAATACCAATCTGCACGGAAT                                                         >  SRR19973071.2215942/1‑150 (MQ=60)

                                                                                              AAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGA                                                       >  SRR19973071.595276/1‑150 (MQ=60)

                                                                                              AAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGA                                                       >  SRR19973071.595392/1‑150 (MQ=60)

                                                                                              AAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGA                                                       >  SRR19973071.2690612/1‑150 (MQ=60)

                                                                                               AAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAT                                                      <  SRR19973071.1864116/150‑1 (MQ=60)

                                                                                               AAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGAT                                                      <  SRR19973071.1588952/150‑1 (MQ=60)

                                                                                                 GTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTC                                                    >  SRR19973071.3168385/1‑150 (MQ=60)

                                                                                                   CAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGG                                                  >  SRR19973071.326822/1‑150 (MQ=60)

                                                                                                     GGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGT                                                >  SRR19973071.922859/1‑150 (MQ=60)

                                                                                                     GGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACATGGCAGGCGTCAAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGT                                                >  SRR19973071.4133384/1‑150 (MQ=60)

                                                                                                      GTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTC                                               >  SRR19973071.581187/1‑150 (MQ=60)

                                                                                                      GTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTC                                               >  SRR19973071.581109/1‑150 (MQ=60)

                                                                                                          ACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATC                                           >  SRR19973071.4330646/1‑150 (MQ=60)

                                                                                                            CGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAG                                         >  SRR19973071.2977326/1‑150 (MQ=60)

                                                                                                            CGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAG                                         >  SRR19973071.4249400/1‑150 (MQ=60)

                                                                                                            CGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAG                                         >  SRR19973071.3978085/1‑150 (MQ=60)

                                                                                                                ATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCT                                     >  SRR19973071.1432643/1‑150 (MQ=60)

                                                                                                                ATATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCT                                     >  SRR19973071.1432917/1‑150 (MQ=60)

                                                                                                                 TATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTT                                    <  SRR19973071.1496231/150‑1 (MQ=60)

                                                                                                                  ATCACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTC                                   >  SRR19973071.4648166/1‑150 (MQ=60)

                                                                                                                    CACCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTT                                 >  SRR19973071.9991/1‑150 (MQ=60)

                                                                                                                      CCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTC                               <  SRR19973071.3961709/150‑1 (MQ=60)

                                                                                                                      CCCACAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTC                               <  SRR19973071.1564137/150‑1 (MQ=60)

                                                                                                                        CACAATCTGTTTCACTTGGGCGATAACGTACTCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGACTGATTCGGGTCGATCAGACCTTCTTTCGG                             >  SRR19973071.265187/1‑150 (MQ=60)

                                                                                                                          CAATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCG                           >  SRR19973071.1529510/1‑150 (MQ=60)

                                                                                                                            ATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCG                         >  SRR19973071.953189/1‑150 (MQ=60)

                                                                                                                            ATCTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCG                         >  SRR19973071.3064013/1‑150 (MQ=60)

                                                                                                                              CTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGT                       >  SRR19973071.1634403/1‑150 (MQ=60)

                                                                                                                              CTGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGT                       >  SRR19973071.1634159/1‑150 (MQ=60)

                                                                                                                              ATGTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGAAAAGCCGTTGTCTTTATCAAAATCGGTACCAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTACTTTCGGCGCGGT                       >  SRR19973071.1634154/1‑150 (MQ=60)

                                                                                                                                GTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTCT                     >  SRR19973071.4157735/1‑150 (MQ=60)

                                                                                                                                GTTTCACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTAT                     >  SRR19973071.466028/1‑150 (MQ=60)

                                                                                                                                     ACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGACTACCAATCTGCACGGAATGATTAGGGTCGAGCAGACCATCATTCGGCGCGGTATAGAAC                >  SRR19973071.1311014/1‑150 (MQ=60)

                                                                                                                                     ACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAAC                >  SRR19973071.429138/1‑150 (MQ=60)

                                                                                                                                     ACTTGGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTATTTCGGCGCGGTATAGAAC                >  SRR19973071.429855/1‑150 (MQ=60)

                                                                                                                                         GGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAG            <  SRR19973071.1614778/150‑1 (MQ=60)

                                                                                                                                         GGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAG            <  SRR19973071.1613878/150‑1 (MQ=60)

                                                                                                                                         GGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAG            <  SRR19973071.1613872/150‑1 (MQ=60)

                                                                                                                                         GGGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAG            <  SRR19973071.3324974/150‑1 (MQ=60)

                                                                                                                                          GGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGT           >  SRR19973071.1378569/1‑150 (MQ=60)

                                                                                                                                          GGCGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGT           >  SRR19973071.1377767/1‑150 (MQ=60)

                                                                                                                                            CGATAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGC         >  SRR19973071.1234032/1‑150 (MQ=60)

                                                                                                                                               TAACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGT      >  SRR19973071.4289670/1‑150 (MQ=60)

                                                                                                                                                AACGTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTAGGCGCGGTATAGAACATAGTGCCGTG     >  SRR19973071.4591585/1‑150 (MQ=60)

                                                                                                                                                 ACGTAATCCACGCTGCGATCGGTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGG    <  SRR19973071.4601196/150‑1 (MQ=60)

                                                                                                                                                   GTAATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTC  <  SRR19973071.1037826/150‑1 (MQ=60)

GCGTACCAGTTTAATAGCGCGATCGGAGGTCAGGCCGCCAATCACTGGCGTACCGGTGCCTGGTGCAAAAGCAGGATCCAGGCAGTCGATATCAAAAGTCAGGTAAACCGGCATATCACCCACAATCTGTTTCACTTGGGCGATAACGTCATCCACGCTGCGATCGTTCACCTGGCAGGCGTCCAGCACGGTAAAGCCGTTGTCTTTATCAAACTCGGTACGAATACCAATCTGCACGGAATGATTCGGGTCGATCAGACCTTCTTTCGGCGCGGTATAGAACATAGTGCCGTGGTC  >  CP009273/3076377‑3076673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`