Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,120,216 C→A 91.3% D389Y (GAT→TAT)  glcD ← glycolate oxidase subunit, FAD‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,120,2160CA91.3% 617.4 / 43.2 203D389Y (GAT→TAT) glcDglycolate oxidase subunit, FAD‑linked
Reads supporting (aligned to +/- strand):  ref base C (9/9);  new base A (97/88);  total (106/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 205 total aligned reads displayed.

GATTTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAAC  >  CP009273/3120067‑3120361
                                                                                                                                                     |                                                                                                                                                 
  ttttttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCAAACTTCAACGCAGTGTTCGAGGATCTTCCCGACCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCAtatc                                                                                                                                                 <  1:4499714/150‑1 (MQ=255)
   tttttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATcc                                                                                                                                                <  2:3635598/150‑1 (MQ=255)
     tttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTACCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCgg                                                                                                                                              >  2:1901438/1‑150 (MQ=255)
        ctcGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCAt                                                                                                                                           >  2:4445833/1‑150 (MQ=255)
             ccGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAg                                                                                                                                      <  2:5019117/150‑1 (MQ=255)
             ccGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAg                                                                                                                                      <  2:135616/150‑1 (MQ=255)
              cGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGa                                                                                                                                     >  2:1859055/1‑150 (MQ=255)
              cGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGa                                                                                                                                     >  1:3979242/1‑150 (MQ=255)
               gATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGAc                                                                                                                                    <  1:385620/150‑1 (MQ=255)
                aTGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACg                                                                                                                                   <  2:2832843/150‑1 (MQ=255)
                   ccATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAATAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTg                                                                                                                                <  2:267011/150‑1 (MQ=255)
                   ccATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTg                                                                                                                                <  2:3844197/150‑1 (MQ=255)
                   ccATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTg                                                                                                                                <  2:4121572/150‑1 (MQ=255)
                   ccATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTg                                                                                                                                >  2:2279920/1‑150 (MQ=255)
                   ccATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTg                                                                                                                                <  1:4641580/150‑1 (MQ=255)
                     aTGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGc                                                                                                                              >  1:3817294/1‑150 (MQ=255)
                        ttCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAac                                                                                                                           >  2:640928/1‑150 (MQ=255)
                            ccACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGt                                                                                                                       >  2:864398/1‑150 (MQ=255)
                             cACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTa                                                                                                                      >  1:1122451/1‑150 (MQ=255)
                                  aTGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCa                                                                                                                 <  2:5031523/150‑1 (MQ=255)
                                  aTGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCa                                                                                                                 <  2:4997364/150‑1 (MQ=255)
                                  aTGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCa                                                                                                                 <  2:5031856/150‑1 (MQ=255)
                                  aTGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCa                                                                                                                 <  2:3473221/150‑1 (MQ=255)
                                  aTGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCa                                                                                                                 >  1:1702223/1‑150 (MQ=255)
                                   tgctgcCGCCAACTTCAACGCAGAGTTCGAGGATCGTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCat                                                                                                                <  2:760556/150‑1 (MQ=255)
                                    gcggccgccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTGCCGCGCGGGCAACGTCACCGGGTTCGTTGGCATCGACAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCata                                                                                                               <  2:4891047/147‑1 (MQ=255)
                                      tgcCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATAtt                                                                                                             <  2:182648/150‑1 (MQ=255)
                                         cgccAACTTCCACGCAGAGTTCGAGGATCGTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGATAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgct                                                                                                          <  2:1735272/150‑1 (MQ=255)
                                          gccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgctg                                                                                                         <  2:821109/150‑1 (MQ=255)
                                            cAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCg                                                                                                       <  1:2506536/150‑1 (MQ=255)
                                              aCTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGAt                                                                                                     >  2:1595350/1‑150 (MQ=255)
                                              aCTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGAt                                                                                                     >  2:384733/1‑150 (MQ=255)
                                              aCTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGAt                                                                                                     >  2:3426083/1‑150 (MQ=255)
                                               cTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATa                                                                                                    >  2:2289981/1‑150 (MQ=255)
                                               cTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATa                                                                                                    <  2:4703404/150‑1 (MQ=255)
                                                 tCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATaaa                                                                                                  <  1:4865508/150‑1 (MQ=255)
                                                  cAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAAc                                                                                                 <  1:4907974/150‑1 (MQ=255)
                                                  cAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAAc                                                                                                 <  2:4018793/150‑1 (MQ=255)
                                                    aCGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACgg                                                                                               <  1:2244576/150‑1 (MQ=255)
                                                    aCGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACgg                                                                                               <  1:3576174/150‑1 (MQ=255)
                                                      gCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGc                                                                                             <  2:2641480/150‑1 (MQ=255)
                                                          agTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCACCACGTAAATCATATTGCTGCGATAAACGGGCAATg                                                                                         >  1:4877980/1‑150 (MQ=255)
                                                           gTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACTGGCAATGc                                                                                        >  1:3239370/1‑150 (MQ=255)
                                                           gTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGc                                                                                        >  2:1592386/1‑150 (MQ=255)
                                                           gTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGc                                                                                        <  1:4322752/150‑1 (MQ=255)
                                                           gTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGc                                                                                        >  1:1637165/1‑150 (MQ=255)
                                                           gTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGc                                                                                        >  1:811716/1‑150 (MQ=255)
                                                             tCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCt                                                                                      >  1:280003/1‑150 (MQ=255)
                                                             tCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCt                                                                                      <  2:4817909/150‑1 (MQ=255)
                                                               gAGGATCGTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTc                                                                                    <  1:1799823/150‑1 (MQ=255)
                                                                   aTCGTCCCGCCCAGCTCGTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGt                                                                                <  2:1179297/150‑1 (MQ=255)
                                                                     cTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTAc                                                                              >  1:1022072/1‑150 (MQ=255)
                                                                      ttCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACg                                                                             >  2:2892976/1‑150 (MQ=255)
                                                                      ttCCCGCCCAGCTCTTCCGCGCGGGCAAAGTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACg                                                                             <  2:2104916/150‑1 (MQ=255)
                                                                        cccGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGcc                                                                           >  2:4268827/1‑150 (MQ=255)
                                                                                 ctctTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGgc                                                                  >  1:2869943/1‑150 (MQ=255)
                                                                                 ctctTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGgc                                                                  <  2:1115177/150‑1 (MQ=255)
                                                                                   ctTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGgcgc                                                                >  2:378508/1‑150 (MQ=255)
                                                                                       cgcgcgGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGAcg                                                            >  2:3584206/1‑150 (MQ=255)
                                                                                          gcgGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCgg                                                         <  2:3198861/150‑1 (MQ=255)
                                                                                             ggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGAt                                                      >  1:875868/1‑150 (MQ=255)
                                                                                              gCAAATTCACCGGGTTCGTTGGCATCGACAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATg                                                     <  2:4204784/150‑1 (MQ=255)
                                                                                              gCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATg                                                     <  2:1610714/150‑1 (MQ=255)
                                                                                              gCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATg                                                     <  2:1610617/150‑1 (MQ=255)
                                                                                                aaaTTCACCGGGGTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGt                                                   <  2:3574606/150‑1 (MQ=255)
                                                                                                 aaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTg                                                  >  1:1337076/1‑150 (MQ=255)
                                                                                                 aaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTg                                                  >  1:1336965/1‑150 (MQ=255)
                                                                                                   ttCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGcc                                                >  1:3114049/1‑150 (MQ=255)
                                                                                                     naCCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGNTGCGATAAACGGGCAATNCCTNCNAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccat                                              >  2:2692504/2‑150 (MQ=255)
                                                                                                     cACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccat                                              >  1:868163/1‑150 (MQ=255)
                                                                                                       ccGGGGTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatcc                                            <  1:5192371/150‑1 (MQ=255)
                                                                                                           gTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGc                                        >  1:4362985/1‑150 (MQ=255)
                                                                                                             tCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCag                                      >  2:225612/1‑150 (MQ=255)
                                                                                                              cGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagt                                     >  2:2954849/1‑150 (MQ=255)
                                                                                                              cGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagt                                     >  2:4295912/1‑150 (MQ=255)
                                                                                                               gTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagta                                    >  2:456822/1‑150 (MQ=255)
                                                                                                                ttGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagtag                                   >  2:4579709/1‑150 (MQ=255)
                                                                                                                 tGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagtagt                                  >  2:4939535/1‑150 (MQ=255)
                                                                                                                 tGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagtagt                                  <  1:1305243/150‑1 (MQ=255)
                                                                                                                  ggCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAgtagta                                 >  1:1370141/1‑150 (MQ=255)
                                                                                                                   gCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTaa                                <  2:4048236/150‑1 (MQ=255)
                                                                                                                       cGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCg                            >  1:4250539/1‑150 (MQ=255)
                                                                                                                       cGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCg                            <  2:2165652/150‑1 (MQ=255)
                                                                                                                        gAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCgg                           >  1:2129453/1‑150 (MQ=255)
                                                                                                                            aGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCAGGgag                       <  2:5161710/150‑1 (MQ=255)
                                                                                                                              gATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAt                     <  1:4968718/150‑1 (MQ=255)
                                                                                                                               aTTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGgagaca                    <  1:1985293/150‑3 (MQ=255)
                                                                                                                                 tAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACg                  >  1:4069042/1‑150 (MQ=255)
                                                                                                                                 tAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACg                  >  2:3459958/1‑150 (MQ=255)
                                                                                                                                  aaCGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGt                 >  1:4951004/1‑150 (MQ=255)
                                                                                                                                     gggTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCt              <  2:1450290/150‑1 (MQ=255)
                                                                                                                                     gggTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCt              >  2:2082377/1‑150 (MQ=255)
                                                                                                                                      ggTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTa             >  1:1179101/1‑150 (MQ=255)
                                                                                                                                         gCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccg          <  1:341858/150‑1 (MQ=255)
                                                                                                                                           aTGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgcc        >  1:4187102/1‑150 (MQ=255)
                                                                                                                                             gTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCgg      <  1:1712319/150‑1 (MQ=255)
                                                                                                                                                gCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGaa   >  2:2465074/1‑150 (MQ=255)
                                                                                                                                                 ccATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAAc  <  2:4524801/150‑1 (MQ=255)
                                                                                                                                                 ccATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAAc  >  2:3103590/1‑150 (MQ=255)
                                                                                                                                                 ccATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAAc  <  2:1936756/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GATTTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAAC  >  CP009273/3120067‑3120361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGC  >  CP009273/3120067‑3120363
                                                                                                                                                     |                                                                                                                                                   
GATTTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATC                                                                                                                                                     <  SRR19973083.1138763/150‑1 (MQ=60)
GATTTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATA                                                                                                                                                     >  SRR19973083.3988272/1‑150 (MQ=60)
 ATTTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATAT                                                                                                                                                    >  SRR19973083.2954010/1‑150 (MQ=60)
 ATTTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATAT                                                                                                                                                    >  SRR19973083.1586613/1‑150 (MQ=60)
 ATATTTTCTCGCCCGATGCCATGTTCGCCAATGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGAATATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATAT                                                                                                                                                    <  SRR19973083.1672634/150‑1 (MQ=60)
  TTTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCAAACTTCAACGCAGTGTTCGAGGATCTTCCCGACCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATC                                                                                                                                                   <  SRR19973083.4519246/150‑1 (MQ=60)
   TTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCC                                                                                                                                                  <  SRR19973083.4311869/150‑1 (MQ=60)
     TTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGG                                                                                                                                                >  SRR19973083.370315/1‑150 (MQ=60)
      TTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGC                                                                                                                                               >  SRR19973083.826044/1‑150 (MQ=60)
         TCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATG                                                                                                                                            >  SRR19973083.4266004/1‑150 (MQ=60)
          CGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGA                                                                                                                                           >  SRR19973083.4906176/1‑150 (MQ=60)
            CCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAA                                                                                                                                         <  SRR19973083.4569633/150‑1 (MQ=60)
             CCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAG                                                                                                                                        <  SRR19973083.4940815/150‑1 (MQ=60)
              CGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGA                                                                                                                                       >  SRR19973083.3996847/1‑150 (MQ=60)
              CGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGA                                                                                                                                       >  SRR19973083.3996948/1‑150 (MQ=60)
               GATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGAC                                                                                                                                      <  SRR19973083.387402/150‑1 (MQ=60)
                   CCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTG                                                                                                                                  <  SRR19973083.4661584/150‑1 (MQ=60)
                     ATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGC                                                                                                                                >  SRR19973083.3834454/1‑150 (MQ=60)
                     ATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGC                                                                                                                                >  SRR19973083.3834469/1‑150 (MQ=60)
                     ATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGC                                                                                                                                >  SRR19973083.4974813/1‑150 (MQ=60)
                     ATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGC                                                                                                                                >  SRR19973083.3446845/1‑150 (MQ=60)
                     ATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGC                                                                                                                                >  SRR19973083.3479479/1‑150 (MQ=60)
                             CACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTA                                                                                                                        >  SRR19973083.1128087/1‑150 (MQ=60)
                             CACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTA                                                                                                                        >  SRR19973083.74747/1‑150 (MQ=60)
                                 GATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATC                                                                                                                    <  SRR19973083.1735214/150‑1 (MQ=60)
                                  ATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCA                                                                                                                   >  SRR19973083.1710605/1‑150 (MQ=60)
                                            CAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCG                                                                                                         <  SRR19973083.5106894/150‑1 (MQ=60)
                                            CAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCG                                                                                                         <  SRR19973083.2519304/150‑1 (MQ=60)
                                                 TCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAA                                                                                                    <  SRR19973083.4886395/150‑1 (MQ=60)
                                                 TCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAA                                                                                                    <  SRR19973083.4886421/150‑1 (MQ=60)
                                                  CAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAAC                                                                                                   <  SRR19973083.4929075/150‑1 (MQ=60)
                                                  CAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAAC                                                                                                   >  SRR19973083.2694589/1‑150 (MQ=60)
                                                  CAACGCAGAGTTCGAGGATCGTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAAC                                                                                                   <  SRR19973083.1349886/150‑1 (MQ=60)
                                                    ACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGG                                                                                                 <  SRR19973083.3592566/150‑1 (MQ=60)
                                                    ACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGG                                                                                                 <  SRR19973083.2255986/150‑1 (MQ=60)
                                                          AGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCACCACGTAAATCATATTGCTGCGATAAACGGGCAATG                                                                                           >  SRR19973083.4898928/1‑150 (MQ=60)
                                                           GTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACTGGCAATGC                                                                                          >  SRR19973083.3254702/1‑150 (MQ=60)
                                                           GTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGC                                                                                          >  SRR19973083.815591/1‑150 (MQ=60)
                                                           GTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGC                                                                                          <  SRR19973083.1117307/150‑1 (MQ=60)
                                                           GTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGC                                                                                          <  SRR19973083.4341516/150‑1 (MQ=60)
                                                           GTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGC                                                                                          <  SRR19973083.4341562/150‑1 (MQ=60)
                                                           GTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGC                                                                                          >  SRR19973083.1645267/1‑150 (MQ=60)
                                                           GTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGC                                                                                          >  SRR19973083.1645320/1‑150 (MQ=60)
                                                             TCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCT                                                                                        >  SRR19973083.281475/1‑150 (MQ=60)
                                                               GAGGATCGTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTC                                                                                      <  SRR19973083.1808835/150‑1 (MQ=60)
                                                                 GGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCA                                                                                    <  SRR19973083.2217480/150‑1 (MQ=60)
                                                                  GATCTTCCCGCCCAGCTCTTCCTCGCGGGCAAATTCACCGGGTTCTTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCTATAAAAGGGCAATGCCTTCCAG                                                                                   >  SRR19973083.1665291/1‑150 (MQ=60)
                                                                     CTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTAC                                                                                >  SRR19973083.1027193/1‑150 (MQ=60)
                                                                               AGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCATCACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGG                                                                      >  SRR19973083.3351649/1‑150 (MQ=60)
                                                                                 CTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGC                                                                    >  SRR19973083.2884048/1‑150 (MQ=60)
                                                                                 CTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGC                                                                    >  SRR19973083.2884062/1‑150 (MQ=60)
                                                                                   CTTCCGCGCGGGCACATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGC                                                                  <  SRR19973083.4030549/150‑1 (MQ=60)
                                                                                             GGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGAT                                                        >  SRR19973083.879958/1‑150 (MQ=60)
                                                                                                 AATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTG                                                    >  SRR19973083.1343494/1‑150 (MQ=60)
                                                                                                 AATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTG                                                    >  SRR19973083.1343495/1‑150 (MQ=60)
                                                                                                 AATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTG                                                    >  SRR19973083.1343607/1‑150 (MQ=60)
                                                                                                   TTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCC                                                  >  SRR19973083.3128962/1‑150 (MQ=60)
                                                                                                     CACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCAT                                                >  SRR19973083.872214/1‑150 (MQ=60)
                                                                                                     CACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCAT                                                >  SRR19973083.872268/1‑150 (MQ=60)
                                                                                                       CCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCC                                              <  SRR19973083.4839465/150‑1 (MQ=60)
                                                                                                       CCGGGGTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCC                                              <  SRR19973083.5214356/150‑1 (MQ=60)
                                                                                                           GTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGC                                          >  SRR19973083.4381936/1‑150 (MQ=60)
                                                                                                           GTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGC                                          >  SRR19973083.4381919/1‑150 (MQ=60)
                                                                                                                 TGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGT                                    >  SRR19973083.2920948/1‑150 (MQ=60)
                                                                                                                 TGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGT                                    <  SRR19973083.1311352/150‑1 (MQ=60)
                                                                                                                 TGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGT                                    <  SRR19973083.1311522/150‑1 (MQ=60)
                                                                                                                  GGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTA                                   >  SRR19973083.1376922/1‑150 (MQ=60)
                                                                                                                    CATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAAT                                 >  SRR19973083.2037404/1‑150 (MQ=60)
                                                                                                                       CGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCG                              >  SRR19973083.4269119/1‑150 (MQ=60)
                                                                                                                        GAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGG                             <  SRR19973083.3175035/150‑1 (MQ=60)
                                                                                                                        GAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGG                             >  SRR19973083.2140180/1‑150 (MQ=60)
                                                                                                                              GATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAT                       >  SRR19973083.1320056/1‑150 (MQ=60)
                                                                                                                              GATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAT                       <  SRR19973083.4166622/150‑1 (MQ=60)
                                                                                                                              GATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAT                       <  SRR19973083.4990061/150‑1 (MQ=60)
                                                                                                                               ATTATCGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATA                      <  SRR19973083.1995415/150‑1 (MQ=60)
                                                                                                                               ATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGACA                      <  SRR19973083.1995439/150‑1 (MQ=60)
                                                                                                                                 TAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACG                    >  SRR19973083.4087100/1‑150 (MQ=60)
                                                                                                                                  AACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGT                   >  SRR19973083.2400678/1‑150 (MQ=60)
                                                                                                                                  AACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGT                   >  SRR19973083.4972276/1‑150 (MQ=60)
                                                                                                                                      GGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTA               >  SRR19973083.1184954/1‑150 (MQ=60)
                                                                                                                                         GCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCG            <  SRR19973083.952918/150‑1 (MQ=60)
                                                                                                                                         GCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCG            <  SRR19973083.343513/150‑1 (MQ=60)
                                                                                                                                           ATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCC          >  SRR19973083.4205530/1‑150 (MQ=60)
                                                                                                                                             GTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGG        <  SRR19973083.1720525/150‑1 (MQ=60)
                                                                                                                                             GTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGG        <  SRR19973083.1720736/150‑1 (MQ=60)
                                                                                                                                                   ATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGC  >  SRR19973083.1895979/1‑150 (MQ=60)
                                                                                                                                                   ATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGC  >  SRR19973083.880002/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GATTTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGC  >  CP009273/3120067‑3120363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑