Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,120,216 C→A 100% D389Y (GAT→TAT)  glcD ← glycolate oxidase subunit, FAD‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,120,2160CA99.6% 903.0 / ‑4.8 274D389Y (GAT→TAT) glcDglycolate oxidase subunit, FAD‑linked
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (135/138);  total (135/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 275 total aligned reads displayed.

TTTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACG  >  CP009273/3120069‑3120362
                                                                                                                                                   |                                                                                                                                                  
 tttttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATcc                                                                                                                                                 <  2:4276676/150‑1 (MQ=255)
 tttttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATcc                                                                                                                                                 <  1:3709026/150‑1 (MQ=255)
   tttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCgg                                                                                                                                               <  1:2851051/150‑1 (MQ=255)
    ttCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGc                                                                                                                                              >  2:1665804/1‑150 (MQ=255)
       tcGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATg                                                                                                                                           >  2:340676/1‑150 (MQ=255)
        cGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGa                                                                                                                                          <  2:2981316/150‑1 (MQ=255)
        cGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGa                                                                                                                                          >  1:3406579/1‑150 (MQ=255)
        cGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGa                                                                                                                                          >  1:4390395/1‑150 (MQ=255)
        cGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGa                                                                                                                                          >  1:4783480/1‑150 (MQ=255)
        cGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGa                                                                                                                                          >  2:2367366/1‑150 (MQ=255)
         gCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGaa                                                                                                                                         <  2:587287/150‑1 (MQ=255)
            cGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGa                                                                                                                                      <  1:61490/150‑1 (MQ=255)
             gATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGAc                                                                                                                                     <  2:3957182/150‑1 (MQ=255)
              aTGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACg                                                                                                                                    >  2:716356/1‑150 (MQ=255)
              aTGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACg                                                                                                                                    <  2:1432889/150‑1 (MQ=255)
               tGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGt                                                                                                                                   <  2:3838784/150‑1 (MQ=255)
                gCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGtt                                                                                                                                  <  1:1177262/150‑1 (MQ=255)
                 ccATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTg                                                                                                                                 >  1:4057707/1‑150 (MQ=255)
                 ccATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCAGGCATGAAAGACGTTg                                                                                                                                 <  1:4819104/150‑1 (MQ=255)
                   aTGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGc                                                                                                                               >  1:2346310/1‑150 (MQ=255)
                   aTGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGc                                                                                                                               <  1:2151785/150‑1 (MQ=255)
                    tGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCa                                                                                                                              >  1:1971903/1‑150 (MQ=255)
                      ttCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCCTGAAAGACGTTGGCAac                                                                                                                            >  2:2108809/1‑150 (MQ=255)
                      ttCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAac                                                                                                                            <  1:4144200/150‑1 (MQ=255)
                       tCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAaca                                                                                                                           <  1:1362789/150‑1 (MQ=255)
                        cGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAacac                                                                                                                          <  1:4395629/150‑1 (MQ=255)
                        cGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAacac                                                                                                                          <  1:3591242/150‑1 (MQ=255)
                         gCCACTGATGCTGCCGCCAACTTCAAAGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCAGGCATGAAAGACGTTGGCAACACg                                                                                                                         >  1:3460153/1‑150 (MQ=255)
                          ccACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGt                                                                                                                        >  2:4315927/1‑150 (MQ=255)
                              tGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAAt                                                                                                                    >  1:819402/1‑150 (MQ=255)
                               gATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATc                                                                                                                   >  1:3669795/1‑150 (MQ=255)
                                  gctgcCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCata                                                                                                                <  1:3596088/150‑1 (MQ=255)
                                    tgcCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATAtt                                                                                                              >  1:524687/1‑150 (MQ=255)
                                        gccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgctg                                                                                                          <  1:2477913/150‑1 (MQ=255)
                                         ccAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATtgctgc                                                                                                         <  1:2327374/150‑1 (MQ=255)
                                            aCTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGAt                                                                                                      <  2:4708580/150‑1 (MQ=255)
                                                cAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAAc                                                                                                  >  1:2120077/1‑150 (MQ=255)
                                                cAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAAc                                                                                                  >  1:3313435/1‑150 (MQ=255)
                                                   cGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACggg                                                                                               >  1:1828126/1‑150 (MQ=255)
                                                    gCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGc                                                                                              >  2:1385197/1‑150 (MQ=255)
                                                     cAGAGTTCGAGGATCGTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCa                                                                                             <  2:1972582/150‑1 (MQ=255)
                                                        agTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAAGTCACCGGGTTCGTTGGCATAGAACAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATg                                                                                          <  2:856631/150‑1 (MQ=255)
                                                         gTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGc                                                                                         >  1:1968659/1‑150 (MQ=255)
                                                            cGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCtt                                                                                      >  2:1974885/1‑150 (MQ=255)
                                                            cGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCtt                                                                                      <  2:1840082/150‑1 (MQ=255)
                                                             gAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTc                                                                                     <  2:1307774/150‑1 (MQ=255)
                                                              aGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTcc                                                                                    >  2:4954977/1‑150 (MQ=255)
                                                                gATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAg                                                                                  <  2:2447434/150‑1 (MQ=255)
                                                                 aTCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGt                                                                                 >  2:4809370/1‑150 (MQ=255)
                                                                           ccAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggca                                                                       >  2:978135/1‑150 (MQ=255)
                                                                             aGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggcagg                                                                     >  2:3753981/1‑150 (MQ=255)
                                                                             aGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggcagg                                                                     >  2:3283540/1‑150 (MQ=255)
                                                                             aGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCcaggcagg                                                                     <  2:2544154/150‑1 (MQ=255)
                                                                              gCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAggg                                                                    <  2:1027622/150‑1 (MQ=255)
                                                                               ctctTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGgc                                                                   >  2:1607514/1‑150 (MQ=255)
                                                                                 ctTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGgcgc                                                                 >  2:4175440/1‑150 (MQ=255)
                                                                                    ccGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGAc                                                              >  1:1397618/1‑150 (MQ=255)
                                                                                        gcgGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCgg                                                          <  1:1707437/150‑1 (MQ=255)
                                                                                         cgGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCggg                                                         >  2:451524/1‑150 (MQ=255)
                                                                                          gggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGa                                                        >  2:4867888/1‑150 (MQ=255)
                                                                                          gggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGa                                                        <  2:966378/150‑1 (MQ=255)
                                                                                          gggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGa                                                        >  2:2573382/1‑150 (MQ=255)
                                                                                           ggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGAt                                                       >  2:5017923/1‑150 (MQ=255)
                                                                                           ggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGAt                                                       <  1:873943/150‑1 (MQ=255)
                                                                                            gCAAATTCACCGGGTTCGTTGGCATCGACAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATg                                                      <  2:527024/150‑1 (MQ=255)
                                                                                            gCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATg                                                      <  2:169242/150‑1 (MQ=255)
                                                                                             cAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATgg                                                     >  1:3031160/1‑150 (MQ=255)
                                                                                              aaaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGt                                                    >  1:4603472/1‑150 (MQ=255)
                                                                                              aaaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGt                                                    <  1:2184191/150‑1 (MQ=255)
                                                                                              aaaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGt                                                    >  1:1150438/1‑150 (MQ=255)
                                                                                              aaaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGt                                                    <  2:394731/150‑1 (MQ=255)
                                                                                               aaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTg                                                   >  2:4438073/1‑150 (MQ=255)
                                                                                               aaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTg                                                   <  1:2684944/150‑1 (MQ=255)
                                                                                               aaTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTg                                                   >  1:3263881/1‑150 (MQ=255)
                                                                                                aTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGc                                                  >  1:656965/1‑150 (MQ=255)
                                                                                                  tCACCGGGGTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGcca                                                <  1:4888221/150‑1 (MQ=255)
                                                                                                    aCCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatc                                              >  2:4628771/1‑150 (MQ=255)
                                                                                                    aCCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatc                                              >  2:4665593/1‑150 (MQ=255)
                                                                                                    aCCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatc                                              <  2:824271/150‑1 (MQ=255)
                                                                                                     ccGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatcc                                             <  1:1565606/150‑1 (MQ=255)
                                                                                                       gggTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGccatccat                                           >  2:3255829/1‑150 (MQ=255)
                                                                                                        ggTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATg                                          <  2:3109479/150‑1 (MQ=255)
                                                                                                          ttCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCa                                        >  2:529178/1‑150 (MQ=255)
                                                                                                          ttCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCa                                        >  1:4735/1‑150 (MQ=255)
                                                                                                           tCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCag                                       <  2:2950054/150‑1 (MQ=255)
                                                                                                            cGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagt                                      <  1:746580/150‑1 (MQ=255)
                                                                                                              ttGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagtag                                    <  1:1495129/150‑1 (MQ=255)
                                                                                                               tGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCagtagt                                   <  2:4905538/150‑1 (MQ=255)
                                                                                                                   aTCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATc                               >  2:121184/1‑150 (MQ=255)
                                                                                                                     cGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTACTCCg                             >  2:1632320/1‑150 (MQ=255)
                                                                                                                      gAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCgg                            >  1:4278011/1‑150 (MQ=255)
                                                                                                                       aaaaGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCggg                           <  2:2644046/150‑1 (MQ=255)
                                                                                                                          aGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGgag                        >  2:3539463/1‑150 (MQ=255)
                                                                                                                            gATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAt                      >  2:4287841/1‑150 (MQ=255)
                                                                                                                                      tGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAcc            <  2:2758731/150‑1 (MQ=255)
                                                                                                                                        cATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgc          <  1:393211/150‑1 (MQ=255)
                                                                                                                                        cATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgc          >  2:2209761/1‑150 (MQ=255)
                                                                                                                                         aTGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAccgcc         <  2:1904780/150‑1 (MQ=255)
                                                                                                                                           gTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCgg       <  1:1665815/150‑1 (MQ=255)
                                                                                                                                             tGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGTTACGTCCTACCGCCGGGa     <  2:2281261/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACG  >  CP009273/3120069‑3120362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCAT  >  CP009273/3120070‑3120365
                                                                                                                                                  |                                                                                                                                                     
TTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCC                                                                                                                                                    <  SRR19973063.3720887/150‑1 (MQ=60)
TTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCC                                                                                                                                                    <  SRR19973063.3721060/150‑1 (MQ=60)
 TTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGTGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCG                                                                                                                                                   <  SRR19973063.4762604/150‑1 (MQ=60)
 TTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCG                                                                                                                                                   <  SRR19973063.3352073/150‑1 (MQ=60)
 TTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCG                                                                                                                                                   <  SRR19973063.4762600/150‑1 (MQ=60)
  TTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGG                                                                                                                                                  >  SRR19973063.2141949/1‑150 (MQ=60)
  TTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGG                                                                                                                                                  <  SRR19973063.3700488/150‑1 (MQ=60)
  TTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGG                                                                                                                                                  <  SRR19973063.2860193/150‑1 (MQ=60)
   TTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGC                                                                                                                                                 >  SRR19973063.169763/1‑150 (MQ=60)
    TCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCA                                                                                                                                                <  SRR19973063.4188680/150‑1 (MQ=60)
       CGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGA                                                                                                                                             >  SRR19973063.4404452/1‑150 (MQ=60)
       CGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGA                                                                                                                                             >  SRR19973063.4798757/1‑150 (MQ=60)
       CGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGA                                                                                                                                             >  SRR19973063.3417414/1‑150 (MQ=60)
        GCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAA                                                                                                                                            <  SRR19973063.1173357/150‑1 (MQ=60)
          CCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAG                                                                                                                                          >  SRR19973063.2431320/1‑150 (MQ=60)
           CGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGA                                                                                                                                         <  SRR19973063.61701/150‑1 (MQ=60)
           CGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGGTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGA                                                                                                                                         >  SRR19973063.7492/1‑150 (MQ=60)
            GATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGAC                                                                                                                                        >  SRR19973063.1560541/1‑150 (MQ=60)
               GCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTT                                                                                                                                     >  SRR19973063.4405355/1‑150 (MQ=60)
               GCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTT                                                                                                                                     <  SRR19973063.1180816/150‑1 (MQ=60)
                CCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTG                                                                                                                                    >  SRR19973063.4070712/1‑150 (MQ=60)
                CCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCAGGCATGAAAGACGTTG                                                                                                                                    <  SRR19973063.4834527/150‑1 (MQ=60)
                  ATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGC                                                                                                                                  <  SRR19973063.2158524/150‑1 (MQ=60)
                  ATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGC                                                                                                                                  >  SRR19973063.2353779/1‑150 (MQ=60)
                  ATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGC                                                                                                                                  >  SRR19973063.2353817/1‑150 (MQ=60)
                   TGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCA                                                                                                                                 >  SRR19973063.1978070/1‑150 (MQ=60)
                   TGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCA                                                                                                                                 >  SRR19973063.1977811/1‑150 (MQ=60)
                     TTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAAC                                                                                                                               <  SRR19973063.4157454/150‑1 (MQ=60)
                      TCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACA                                                                                                                              >  SRR19973063.4200796/1‑150 (MQ=60)
                      TCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACA                                                                                                                              <  SRR19973063.1366890/150‑1 (MQ=60)
                      TCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACA                                                                                                                              >  SRR19973063.1614861/1‑150 (MQ=60)
                       CGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACAC                                                                                                                             <  SRR19973063.3602956/150‑1 (MQ=60)
                       CGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACAC                                                                                                                             <  SRR19973063.4409710/150‑1 (MQ=60)
                       CGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACAC                                                                                                                             <  SRR19973063.3602757/150‑1 (MQ=60)
                        GCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACG                                                                                                                            >  SRR19973063.3471154/1‑150 (MQ=60)
                        GCCACTGATGCTGCCGCCAACTTCAAAGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCAGGCATGAAAGACGTTGGCAACACG                                                                                                                            >  SRR19973063.3471162/1‑150 (MQ=60)
                           ACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAA                                                                                                                         >  SRR19973063.4986369/1‑150 (MQ=60)
                           ACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAA                                                                                                                         <  SRR19973063.825335/150‑1 (MQ=60)
                             TGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAAT                                                                                                                       >  SRR19973063.821431/1‑150 (MQ=60)
                             TGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAAT                                                                                                                       >  SRR19973063.821770/1‑150 (MQ=60)
                              GATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATC                                                                                                                      >  SRR19973063.3681553/1‑150 (MQ=60)
                                TGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCAT                                                                                                                    >  SRR19973063.2085387/1‑150 (MQ=60)
                                 GCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATA                                                                                                                   <  SRR19973063.3607625/150‑1 (MQ=60)
                                   TGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATT                                                                                                                 >  SRR19973063.526225/1‑150 (MQ=60)
                                   TGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATT                                                                                                                 >  SRR19973063.526251/1‑150 (MQ=60)
                                   TGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCATTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATT                                                                                                                 <  SRR19973063.735280/150‑1 (MQ=60)
                                    GCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTG                                                                                                                >  SRR19973063.4130857/1‑150 (MQ=60)
                                      TGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCT                                                                                                              <  SRR19973063.3481793/150‑1 (MQ=60)
                                       GCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTG                                                                                                             <  SRR19973063.2485837/150‑1 (MQ=60)
                                        CCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGC                                                                                                            <  SRR19973063.2334825/150‑1 (MQ=60)
                                         CAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCG                                                                                                           >  SRR19973063.2877086/1‑150 (MQ=60)
                                             TTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAA                                                                                                       <  SRR19973063.4529926/150‑1 (MQ=60)
                                             TTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAA                                                                                                       >  SRR19973063.616629/1‑150 (MQ=60)
                                               CAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAAC                                                                                                     >  SRR19973063.3324028/1‑150 (MQ=60)
                                               CAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAAC                                                                                                     >  SRR19973063.2126706/1‑150 (MQ=60)
                                                  CGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGG                                                                                                  >  SRR19973063.1833815/1‑150 (MQ=60)
                                                   GCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGC                                                                                                 <  SRR19973063.1389311/150‑1 (MQ=60)
                                                   GCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGC                                                                                                 <  SRR19973063.1389296/150‑1 (MQ=60)
                                                       AGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATG                                                                                             <  SRR19973063.2961264/150‑1 (MQ=60)
                                                       AGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATG                                                                                             <  SRR19973063.3103551/150‑1 (MQ=60)
                                                        GTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGC                                                                                            >  SRR19973063.1974816/1‑150 (MQ=60)
                                                        GTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGC                                                                                            >  SRR19973063.1974817/1‑150 (MQ=60)
                                                        GTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGC                                                                                            >  SRR19973063.1974873/1‑150 (MQ=60)
                                                            GAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTC                                                                                        <  SRR19973063.2552456/150‑1 (MQ=60)
                                                            GAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTC                                                                                        >  SRR19973063.4838972/1‑150 (MQ=60)
                                                             AGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCC                                                                                       <  SRR19973063.2161052/150‑1 (MQ=60)
                                                              GGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCA                                                                                      <  SRR19973063.2075910/150‑1 (MQ=60)
                                                                     CCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCC                                                                               <  SRR19973063.1725103/150‑1 (MQ=60)
                                                                        GCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGG                                                                            >  SRR19973063.2345702/1‑150 (MQ=60)
                                                                        GCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGG                                                                            <  SRR19973063.1712429/150‑1 (MQ=60)
                                                                         CCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGC                                                                           >  SRR19973063.4737269/1‑150 (MQ=60)
                                                                         CCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGC                                                                           <  SRR19973063.1457698/150‑1 (MQ=60)
                                                                         CCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGC                                                                           <  SRR19973063.3632233/150‑1 (MQ=60)
                                                                            AGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGG                                                                        <  SRR19973063.4390589/150‑1 (MQ=60)
                                                                             GCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGG                                                                       <  SRR19973063.2705033/150‑1 (MQ=60)
                                                                               TCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCG                                                                     <  SRR19973063.2208412/150‑1 (MQ=60)
                                                                               TCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCG                                                                     <  SRR19973063.2208420/150‑1 (MQ=60)
                                                                                CTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGC                                                                    <  SRR19973063.341658/150‑1 (MQ=60)
                                                                                 TTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCG                                                                   >  SRR19973063.37179/1‑150 (MQ=60)
                                                                                   CCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGAC                                                                 >  SRR19973063.1401846/1‑150 (MQ=60)
                                                                                   CCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGAC                                                                 >  SRR19973063.4877218/1‑150 (MQ=60)
                                                                                   CCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGAC                                                                 >  SRR19973063.4100238/1‑150 (MQ=60)
                                                                                       GCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGG                                                             <  SRR19973063.1712637/150‑1 (MQ=60)
                                                                                        CGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGG                                                            <  SRR19973063.3520901/150‑1 (MQ=60)
                                                                                         TGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGA                                                           >  SRR19973063.750796/1‑150 (MQ=60)
                                                                                          GGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGAT                                                          <  SRR19973063.876455/150‑1 (MQ=60)
                                                                                           GCAAATTCACCGGGTTCGTTGGCATCGACAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATG                                                         <  SRR19973063.4808415/150‑1 (MQ=60)
                                                                                           GCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATG                                                         >  SRR19973063.3619625/1‑150 (MQ=60)
                                                                                            CAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGG                                                        >  SRR19973063.3040838/1‑150 (MQ=60)
                                                                                             AAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGT                                                       >  SRR19973063.4618197/1‑150 (MQ=60)
                                                                                             AAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGT                                                       >  SRR19973063.1153912/1‑150 (MQ=60)
                                                                                             AAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGT                                                       <  SRR19973063.2191034/150‑1 (MQ=60)
                                                                                             AAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGT                                                       >  SRR19973063.1300010/1‑150 (MQ=60)
                                                                                              AATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTG                                                      <  SRR19973063.2693595/150‑1 (MQ=60)
                                                                                              AATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTG                                                      >  SRR19973063.3274360/1‑150 (MQ=60)
                                                                                               ATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGC                                                     >  SRR19973063.658890/1‑150 (MQ=60)
                                                                                                 TCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCA                                                   >  SRR19973063.3267168/1‑150 (MQ=60)
                                                                                                 TCACCGGGGTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCA                                                   <  SRR19973063.4903991/150‑1 (MQ=60)
                                                                                                   ACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATC                                                 <  SRR19973063.4490825/150‑1 (MQ=60)
                                                                                                    CCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCC                                                <  SRR19973063.1796661/150‑1 (MQ=60)
                                                                                                    CCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCC                                                <  SRR19973063.1570414/150‑1 (MQ=60)
                                                                                                    CCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCC                                                <  SRR19973063.2768901/150‑1 (MQ=60)
                                                                                                         TTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCA                                           >  SRR19973063.3300922/1‑150 (MQ=60)
                                                                                                         TTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCA                                           >  SRR19973063.4759/1‑150 (MQ=60)
                                                                                                           CGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGT                                         <  SRR19973063.748763/150‑1 (MQ=60)
                                                                                                           CGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGT                                         <  SRR19973063.3341630/150‑1 (MQ=60)
                                                                                                             TTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAG                                       <  SRR19973063.1499708/150‑1 (MQ=60)
                                                                                                             TTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAG                                       <  SRR19973063.4047534/150‑1 (MQ=60)
                                                                                                                GCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAA                                    >  SRR19973063.4713421/1‑150 (MQ=60)
                                                                                                                GCATCGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAA                                    >  SRR19973063.4381353/1‑150 (MQ=60)
                                                                                                                    CGAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCG                                >  SRR19973063.2154467/1‑150 (MQ=60)
                                                                                                                     GAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGG                               >  SRR19973063.4291609/1‑150 (MQ=60)
                                                                                                                     GAAAAGGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGG                               >  SRR19973063.2419319/1‑150 (MQ=60)
                                                                                                                          GGATTAACGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGA                          >  SRR19973063.3877523/1‑150 (MQ=60)
                                                                                                                                 CGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCC                   >  SRR19973063.143265/1‑150 (MQ=60)
                                                                                                                                 CGGGTGCATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCC                   <  SRR19973063.1313204/150‑1 (MQ=60)
                                                                                                                                       CATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGC             <  SRR19973063.5123305/150‑1 (MQ=60)
                                                                                                                                       CATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGC             <  SRR19973063.394330/150‑1 (MQ=60)
                                                                                                                                       CATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGC             <  SRR19973063.868165/150‑1 (MQ=60)
                                                                                                                                        ATGTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCC            <  SRR19973063.64206/150‑1 (MQ=60)
                                                                                                                                          GTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGG          <  SRR19973063.1670895/150‑1 (MQ=60)
                                                                                                                                          GTTGCCATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGG          <  SRR19973063.1670884/150‑1 (MQ=60)
                                                                                                                                               CATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACG     <  SRR19973063.924273/150‑1 (MQ=60)
                                                                                                                                                ATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGC    >  SRR19973063.1803505/1‑150 (MQ=60)
                                                                                                                                                ATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGC    >  SRR19973063.1462968/1‑150 (MQ=60)
                                                                                                                                                ATATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGC    >  SRR19973063.1090958/1‑150 (MQ=60)
                                                                                                                                                 TATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCA   <  SRR19973063.4824748/150‑1 (MQ=60)
                                                                                                                                                  ATCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCAT  <  SRR19973063.4842281/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
TTTTTCTCGCCCGATGCCATGTTCGCCACTGATGCTGCCGCCAACTTCAACGCAGAGTTCGAGGATCTTCCCGCCCAGCTCTTCCGCGCGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCAT  >  CP009273/3120070‑3120365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑