Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,056,018 T→A 93.4% F138I (TTC→ATC)  yihN → MFS transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,056,0180TA93.4% 736.9 / 31.8 212F138I (TTC→ATC) yihNMFS transporter family protein
Reads supporting (aligned to +/- strand):  ref base T (4/10);  new base A (101/97);  total (105/107)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 217 total aligned reads displayed.

TTTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTC  >  CP009273/4055869‑4056164
                                                                                                                                                     |                                                                                                                                                  
      tGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTNGGCGATATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGTGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGaaa                                                                                                                                              >  1:4795032/1‑150 (MQ=255)
      tGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGaaa                                                                                                                                              <  1:4250622/150‑1 (MQ=255)
        acgacAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACa                                                                                                                                            <  1:3113029/150‑1 (MQ=255)
          gacAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAgg                                                                                                                                          <  2:2744696/150‑1 (MQ=255)
             aaTGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCg                                                                                                                                       >  1:3305692/1‑150 (MQ=255)
                   ggggTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAt                                                                                                                                 <  1:3704407/150‑1 (MQ=255)
                    gggTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAtt                                                                                                                                <  1:1647662/150‑1 (MQ=255)
                    gggTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAtt                                                                                                                                <  1:3386682/150‑1 (MQ=255)
                     ggTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTg                                                                                                                               >  1:1034272/1‑150 (MQ=255)
                       tATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTc                                                                                                                             <  2:4965360/150‑1 (MQ=255)
                        aTTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCg                                                                                                                            >  2:653464/1‑150 (MQ=255)
                                aTCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtg                                                                                                                    >  2:4044765/1‑150 (MQ=255)
                                       ttGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCAt                                                                                                             >  1:3981260/1‑150 (MQ=255)
                                        tGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCAtt                                                                                                            <  2:1545405/150‑1 (MQ=255)
                                            tGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCt                                                                                                        >  1:4206971/1‑150 (MQ=255)
                                            tGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCt                                                                                                        >  2:3950791/1‑150 (MQ=255)
                                                cgcTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGt                                                                                                    >  2:1109427/1‑150 (MQ=255)
                                                 gcTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGtt                                                                                                   <  1:3466184/150‑1 (MQ=255)
                                                    aTTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGc                                                                                                <  1:1551209/150‑1 (MQ=255)
                                                        ggCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTa                                                                                            >  2:2545139/1‑150 (MQ=255)
                                                          cGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAtt                                                                                          >  2:877823/1‑150 (MQ=255)
                                                           gTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAttt                                                                                         >  2:1679740/1‑150 (MQ=255)
                                                           gTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAttt                                                                                         <  2:4639009/150‑1 (MQ=255)
                                                           gTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAttt                                                                                         >  1:3564504/1‑150 (MQ=255)
                                                           gTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAttt                                                                                         >  1:1168789/1‑150 (MQ=255)
                                                            ttACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTa                                                                                        <  2:712922/150‑1 (MQ=255)
                                                               cTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCt                                                                                     >  2:344659/1‑150 (MQ=255)
                                                               cTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCt                                                                                     >  1:2791335/1‑150 (MQ=255)
                                                               cTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCt                                                                                     <  2:3564444/150‑1 (MQ=255)
                                                                ttGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTg                                                                                    <  1:2981721/150‑1 (MQ=255)
                                                                 tGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTgg                                                                                   <  1:792610/150‑1 (MQ=255)
                                                                   cGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGtt                                                                                 <  1:873919/150‑1 (MQ=255)
                                                                    gACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGttt                                                                                >  2:3521832/1‑150 (MQ=255)
                                                                    gACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGttt                                                                                >  2:762428/1‑150 (MQ=255)
                                                                     aCATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTg                                                                               >  1:3746308/1‑150 (MQ=255)
                                                                      cATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTgg                                                                              <  1:3412659/150‑1 (MQ=255)
                                                                       atgatgAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGTGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGc                                                                             >  2:4625869/1‑150 (MQ=255)
                                                                          atgaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGAGGTGGCATTTTCTGCGTTGGCAGTAGTTACCTGGTTTGGCAGt                                                                          >  2:4048173/1‑150 (MQ=255)
                                                                           tgaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTg                                                                         <  2:1941055/150‑1 (MQ=255)
                                                                           tgaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTg                                                                         <  1:4871700/150‑1 (MQ=255)
                                                                            gaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTgg                                                                        >  1:1874007/1‑150 (MQ=255)
                                                                            gaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTgg                                                                        >  1:2492879/1‑150 (MQ=255)
                                                                             aaCTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGc                                                                       >  1:3585268/1‑150 (MQ=255)
                                                                               cTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtt                                                                     <  2:3786803/150‑1 (MQ=255)
                                                                               cTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtt                                                                     <  2:2904051/150‑1 (MQ=255)
                                                                                tGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtta                                                                    <  2:1014005/150‑1 (MQ=255)
                                                                                tGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtta                                                                    >  1:2296742/1‑150 (MQ=255)
                                                                                 ggCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCttat                                                                   <  2:3205753/150‑1 (MQ=255)
                                                                                 ggCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCttat                                                                   <  1:4765070/150‑1 (MQ=255)
                                                                                    cGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAg                                                                           <  1:4993872/139‑1 (MQ=255)
                                                                                    cGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAg                                                                >  1:1568515/1‑150 (MQ=255)
                                                                                     ggTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAgg                                                               >  1:1499338/1‑150 (MQ=255)
                                                                                     ggTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAgg                                                               <  1:4146375/150‑1 (MQ=255)
                                                                                      gTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGt                                                              <  2:1878558/150‑1 (MQ=255)
                                                                                        cTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGttt                                                            <  2:3624823/150‑1 (MQ=255)
                                                                                        cTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGttt                                                            >  2:4599745/1‑150 (MQ=255)
                                                                                        cTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCGGGTTTGGCAGTGGCTTATTAGGttt                                                            >  2:3066155/1‑150 (MQ=255)
                                                                                         ttGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGtttt                                                           <  1:3084339/150‑1 (MQ=255)
                                                                                         ttGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGtttt                                                           >  1:4323149/1‑150 (MQ=255)
                                                                                          tGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTa                                                          <  2:2488723/150‑1 (MQ=255)
                                                                                               aaGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCa                                                     <  1:4827102/150‑1 (MQ=255)
                                                                                                aGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAg                                                    >  2:4991745/1‑150 (MQ=255)
                                                                                                aGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAg                                                    >  1:360573/1‑150 (MQ=255)
                                                                                                     gTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATc                                               <  2:1323150/150‑1 (MQ=255)
                                                                                                      tAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCt                                              >  2:2942963/1‑150 (MQ=255)
                                                                                                           cGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTc                                         <  1:3902098/150‑1 (MQ=255)
                                                                                                            gATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCt                                        <  2:1356445/150‑1 (MQ=255)
                                                                                                             aTTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTa                                       <  2:4683663/150‑1 (MQ=255)
                                                                                                             aTTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTa                                       >  1:706858/1‑150 (MQ=255)
                                                                                                             aTTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTa                                       <  1:1117149/150‑1 (MQ=255)
                                                                                                              ttGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTAt                                      >  2:2241082/1‑150 (MQ=255)
                                                                                                              ttGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTAt                                      <  1:5101355/150‑1 (MQ=255)
                                                                                                                gggCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTc                                    >  1:556880/1‑150 (MQ=255)
                                                                                                                  gCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCCTTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTccc                                  >  2:253601/1‑150 (MQ=255)
                                                                                                                   cAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCt                                 <  1:1964624/150‑1 (MQ=255)
                                                                                                                    aaTAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCtt                                >  1:2384351/1‑150 (MQ=255)
                                                                                                                     aTAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTa                               >  2:520552/1‑150 (MQ=255)
                                                                                                                       aGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTAtt                             >  1:2331308/1‑150 (MQ=255)
                                                                                                                         cGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATtgt                           >  1:2170185/1‑150 (MQ=255)
                                                                                                                         cGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATtgt                           >  2:4191942/1‑150 (MQ=255)
                                                                                                                          gAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATtgtg                          >  1:2663978/1‑150 (MQ=255)
                                                                                                                            acaacaAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGAt                        <  2:400660/150‑1 (MQ=255)
                                                                                                                            acaacaAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGAt                        <  2:263553/150‑1 (MQ=255)
                                                                                                                             caacaaGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGAtt                       <  2:3147116/150‑1 (MQ=255)
                                                                                                                             caacaaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGAtt                       >  2:180685/1‑150 (MQ=255)
                                                                                                                                caaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGcc                    >  2:1796084/1‑150 (MQ=255)
                                                                                                                                 aaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCg                   >  1:383139/1‑150 (MQ=255)
                                                                                                                                   ggTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTa                 <  1:2879657/150‑1 (MQ=255)
                                                                                                                                    gTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAg                >  1:5092000/1‑150 (MQ=255)
                                                                                                                                     tCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAgg               <  2:4141089/150‑1 (MQ=255)
                                                                                                                                     tCGGTTGTTTGGCGTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAgg               <  2:1988611/150‑1 (MQ=255)
                                                                                                                                      cGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGc              >  1:4363394/1‑150 (MQ=255)
                                                                                                                                      cGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGc              <  2:3529686/150‑1 (MQ=255)
                                                                                                                                           gttTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattat         >  2:4101643/1‑150 (MQ=255)
                                                                                                                                           gttTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattat         <  2:1509846/150‑1 (MQ=255)
                                                                                                                                              tGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattattat      >  1:1586889/1‑150 (MQ=255)
                                                                                                                                              tGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCatgattat      >  1:1213284/1‑150 (MQ=255)
                                                                                                                                               ggCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCGCTTATTGTGATTGCCGTAGGCattattatt     >  1:2235061/1‑150 (MQ=255)
                                                                                                                                               ggCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattattatt     >  1:1335264/1‑150 (MQ=255)
                                                                                                                                                 cttcatCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTAtttt   >  1:4098225/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTC  >  CP009273/4055869‑4056164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTT  >  CP009273/4055870‑4056166
                                                                                                                                                    |                                                                                                                                                    
TTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCAT                                                                                                                                                     >  SRR19973083.2893160/1‑150 (MQ=60)
 TATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATC                                                                                                                                                    <  SRR19973083.2252079/150‑1 (MQ=60)
  ATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCG                                                                                                                                                   <  SRR19973083.3752384/150‑1 (MQ=60)
     TGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTNGGCGATATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGTGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAA                                                                                                                                                >  SRR19973083.4815536/1‑150 (MQ=60)
     TGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAA                                                                                                                                                <  SRR19973083.4269202/150‑1 (MQ=60)
     TGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAA                                                                                                                                                <  SRR19973083.4269198/150‑1 (MQ=60)
       ACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACA                                                                                                                                              <  SRR19973083.3127940/150‑1 (MQ=60)
            AATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCG                                                                                                                                         >  SRR19973083.3321220/1‑150 (MQ=60)
             ATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGT                                                                                                                                        <  SRR19973083.939368/150‑1 (MQ=60)
             ATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGT                                                                                                                                        <  SRR19973083.3120062/150‑1 (MQ=60)
             ATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGT                                                                                                                                        <  SRR19973083.1436164/150‑1 (MQ=60)
                  GGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAT                                                                                                                                   <  SRR19973083.3721276/150‑1 (MQ=60)
                   GGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATT                                                                                                                                  <  SRR19973083.215021/150‑1 (MQ=60)
                   GGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATT                                                                                                                                  <  SRR19973083.1655858/150‑1 (MQ=60)
                   GGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATT                                                                                                                                  <  SRR19973083.3402374/150‑1 (MQ=60)
                    GGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTG                                                                                                                                 >  SRR19973083.1039465/1‑150 (MQ=60)
                     GTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGT                                                                                                                                >  SRR19973083.246278/1‑150 (MQ=60)
                     GTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGT                                                                                                                                >  SRR19973083.1056193/1‑150 (MQ=60)
                        TTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGA                                                                                                                             <  SRR19973083.1204403/150‑1 (MQ=60)
                               ATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTG                                                                                                                      >  SRR19973083.3837897/1‑150 (MQ=60)
                               ATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTG                                                                                                                      >  SRR19973083.5118218/1‑150 (MQ=60)
                                   TCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGG                                                                                                                  >  SRR19973083.954535/1‑150 (MQ=60)
                                   TCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGG                                                                                                                  <  SRR19973083.1623180/150‑1 (MQ=60)
                                      TTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCAT                                                                                                               >  SRR19973083.3998972/1‑150 (MQ=60)
                                      TTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCAT                                                                                                               >  SRR19973083.716464/1‑150 (MQ=60)
                                       TGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATT                                                                                                              <  SRR19973083.3410142/150‑1 (MQ=60)
                                        GTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTT                                                                                                             <  SRR19973083.5076063/150‑1 (MQ=60)
                                           TGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCT                                                                                                          >  SRR19973083.3613514/1‑150 (MQ=60)
                                           TGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCT                                                                                                          >  SRR19973083.1572124/1‑150 (MQ=60)
                                           TGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCT                                                                                                          >  SRR19973083.4225454/1‑150 (MQ=60)
                                                GCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTT                                                                                                     <  SRR19973083.3482046/150‑1 (MQ=60)
                                                GCTATTGGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTT                                                                                                     <  SRR19973083.2801093/150‑1 (MQ=60)
                                                  TATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGG                                                                                                   <  SRR19973083.3394681/150‑1 (MQ=60)
                                                   ATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGC                                                                                                  <  SRR19973083.1558996/150‑1 (MQ=60)
                                                   ATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGC                                                                                                  <  SRR19973083.1559010/150‑1 (MQ=60)
                                                          GTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCGATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTT                                                                                           >  SRR19973083.3630033/1‑150 (MQ=60)
                                                          GTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTT                                                                                           >  SRR19973083.1174599/1‑150 (MQ=60)
                                                          GTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTT                                                                                           >  SRR19973083.3580802/1‑150 (MQ=60)
                                                          GTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTT                                                                                           >  SRR19973083.3580862/1‑150 (MQ=60)
                                                          GTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGACAGTATTT                                                                                           <  SRR19973083.3474527/150‑1 (MQ=60)
                                                              CTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCT                                                                                       >  SRR19973083.2805204/1‑150 (MQ=60)
                                                              CTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCA                                                                                       <  SRR19973083.4992760/150‑1 (MQ=60)
                                                               TTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTG                                                                                      <  SRR19973083.2996174/150‑1 (MQ=60)
                                                                TGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGG                                                                                     <  SRR19973083.796437/150‑1 (MQ=60)
                                                                  CGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTT                                                                                   <  SRR19973083.822815/150‑1 (MQ=60)
                                                                  CGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTT                                                                                   <  SRR19973083.877998/150‑1 (MQ=60)
                                                                  CGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTT                                                                                   <  SRR19973083.4785748/150‑1 (MQ=60)
                                                                    ACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTG                                                                                 >  SRR19973083.3763290/1‑150 (MQ=60)
                                                                     CATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGG                                                                                <  SRR19973083.3428412/150‑1 (MQ=60)
                                                                     CATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGG                                                                                <  SRR19973083.4197313/150‑1 (MQ=60)
                                                                        tcttccgatctCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAG                                                                             <  SRR19973083.5015316/139‑1 (MQ=60)
                                                                          TGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTG                                                                           <  SRR19973083.4892577/150‑1 (MQ=60)
                                                                          TGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTG                                                                           <  SRR19973083.4892620/150‑1 (MQ=60)
                                                                           GAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGG                                                                          >  SRR19973083.2505594/1‑150 (MQ=60)
                                                                           GAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGG                                                                          >  SRR19973083.1883493/1‑150 (MQ=60)
                                                                           GAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGGTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGG                                                                          >  SRR19973083.1883468/1‑150 (MQ=60)
                                                                            AACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGC                                                                         >  SRR19973083.3601344/1‑150 (MQ=60)
                                                                            AACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGC                                                                         >  SRR19973083.3601695/1‑150 (MQ=60)
                                                                               TGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTA                                                                      >  SRR19973083.2308503/1‑150 (MQ=60)
                                                                                GGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTAT                                                                     <  SRR19973083.4785374/150‑1 (MQ=60)
                                                                                GGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTAT                                                                     <  SRR19973083.2942801/150‑1 (MQ=60)
                                                                                   CGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAG                                                                  >  SRR19973083.1576389/1‑150 (MQ=60)
                                                                                    GGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGG                                                                 >  SRR19973083.1506889/1‑150 (MQ=60)
                                                                                    GGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGG                                                                 <  SRR19973083.4164691/150‑1 (MQ=60)
                                                                                    GGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATGTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGG                                                                 >  SRR19973083.4113906/1‑150 (MQ=60)
                                                                                        TTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTT                                                             >  SRR19973083.4341914/1‑150 (MQ=60)
                                                                                        TTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTT                                                             <  SRR19973083.3099125/150‑1 (MQ=60)
                                                                                         TGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTA                                                            >  SRR19973083.4042672/1‑150 (MQ=60)
                                                                                              AAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCA                                                       <  SRR19973083.4847800/150‑1 (MQ=60)
                                                                                              AAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCA                                                       <  SRR19973083.4847806/150‑1 (MQ=60)
                                                                                               AGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAG                                                      >  SRR19973083.362188/1‑150 (MQ=60)
                                                                                               AGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAG                                                      >  SRR19973083.362279/1‑150 (MQ=60)
                                                                                                GTCGGTAAGTAGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGG                                                     <  SRR19973083.23375/150‑1 (MQ=60)
                                                                                                   GGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCAT                                                  >  SRR19973083.1263694/1‑150 (MQ=60)
                                                                                                   GGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCAT                                                  >  SRR19973083.3007167/1‑150 (MQ=60)
                                                                                                       AGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGG                                              >  SRR19973083.2887175/1‑150 (MQ=60)
                                                                                                       AGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTGATTAGGTTTTAAAGCAGGCATCTGG                                              >  SRR19973083.114142/1‑150 (MQ=60)
                                                                                                          CGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTC                                           <  SRR19973083.3919473/150‑1 (MQ=60)
                                                                                                            ATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTA                                         >  SRR19973083.710380/1‑150 (MQ=60)
                                                                                                            ATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTA                                         <  SRR19973083.1122757/150‑1 (MQ=60)
                                                                                                             TTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTAT                                        <  SRR19973083.5123120/150‑1 (MQ=60)
                                                                                                              TGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATT                                       <  SRR19973083.1558930/150‑1 (MQ=60)
                                                                                                              TGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGGGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATT                                       <  SRR19973083.2387311/150‑1 (MQ=60)
                                                                                                               GGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTC                                      >  SRR19973083.559658/1‑150 (MQ=60)
                                                                                                                  CAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCT                                   <  SRR19973083.1974660/150‑1 (MQ=60)
                                                                                                                   AATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTT                                  >  SRR19973083.2396622/1‑150 (MQ=60)
                                                                                                                     TAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTAT                                <  SRR19973083.4755413/150‑1 (MQ=60)
                                                                                                                      AGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATT                               >  SRR19973083.2343272/1‑150 (MQ=60)
                                                                                                                       GCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTG                              >  SRR19973083.2634481/1‑150 (MQ=60)
                                                                                                                       GCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTG                              <  SRR19973083.693691/150‑1 (MQ=60)
                                                                                                                        CGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGT                             >  SRR19973083.2181062/1‑150 (MQ=60)
                                                                                                                        CGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGT                             >  SRR19973083.2181135/1‑150 (MQ=60)
                                                                                                                         GAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTG                            >  SRR19973083.2677351/1‑150 (MQ=60)
                                                                                                                            CAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATT                         <  SRR19973083.2539482/150‑1 (MQ=60)
                                                                                                                             AACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTG                        >  SRR19973083.390501/1‑150 (MQ=60)
                                                                                                                                AAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCG                     <  SRR19973083.5115112/150‑1 (MQ=60)
                                                                                                                                AAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCG                     >  SRR19973083.4734305/1‑150 (MQ=60)
                                                                                                                                AAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCG                     >  SRR19973083.384911/1‑150 (MQ=60)
                                                                                                                                 AGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGT                    >  SRR19973083.5158881/1‑150 (MQ=60)
                                                                                                                                  GGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTA                   <  SRR19973083.2893804/150‑1 (MQ=60)
                                                                                                                                   GTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAG                  >  SRR19973083.5113748/1‑150 (MQ=60)
                                                                                                                                    TCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGG                 <  SRR19973083.1900285/150‑1 (MQ=60)
                                                                                                                                     CGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGC                >  SRR19973083.4382336/1‑150 (MQ=60)
                                                                                                                                     CGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGC                >  SRR19973083.4382293/1‑150 (MQ=60)
                                                                                                                                        TTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATT             >  SRR19973083.3283149/1‑150 (MQ=60)
                                                                                                                                        TTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATT             >  SRR19973083.3283200/1‑150 (MQ=60)
                                                                                                                                           TTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATT          >  SRR19973083.2027246/1‑150 (MQ=60)
                                                                                                                                            TTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTA         <  SRR19973083.3984744/150‑1 (MQ=60)
                                                                                                                                             TGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTAT        >  SRR19973083.1594842/1‑150 (MQ=60)
                                                                                                                                             TGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTAT        >  SRR19973083.4304862/1‑150 (MQ=60)
                                                                                                                                             TGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATGATTAT        >  SRR19973083.1219264/1‑150 (MQ=60)
                                                                                                                                             TGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCGTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCCTTATTAT        >  SRR19973083.1594181/1‑150 (MQ=60)
                                                                                                                                              GGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCGCTTATTGTGATTGCCGTAGGCATTATTATT       >  SRR19973083.2246407/1‑150 (MQ=60)
                                                                                                                                              GGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATT       >  SRR19973083.1341777/1‑150 (MQ=60)
                                                                                                                                                CTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTT     >  SRR19973083.4116375/1‑150 (MQ=60)
                                                                                                                                                CTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCGTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTT     >  SRR19973083.4116462/1‑150 (MQ=60)
                                                                                                                                                   CATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTT  <  SRR19973083.3104692/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTT  >  CP009273/4055870‑4056166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑