Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,056,018 T→A 100% F138I (TTC→ATC)  yihN → MFS transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,056,0180TA100.0% 685.4 / NA 205F138I (TTC→ATC) yihNMFS transporter family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (85/120);  total (85/120)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

TTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTAT  >  CP009273/4055873‑4056160
                                                                                                                                                 |                                                                                                                                              
tttGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCttcatc                                                                                                                                              >  1:333563/1‑148 (MQ=255)
       acAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAg                                                                                                                                       >  2:2499543/1‑148 (MQ=255)
       acAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAg                                                                                                                                       >  1:3017542/1‑148 (MQ=255)
        cAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAgg                                                                                                                                      <  2:2353006/148‑1 (MQ=255)
        cAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAgg                                                                                                                                      <  2:589020/148‑1 (MQ=255)
          aTGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGc                                                                                                                                    >  1:2259791/1‑148 (MQ=255)
          aTGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGc                                                                                                                                    >  2:1065117/1‑148 (MQ=255)
           tGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCg                                                                                                                                   >  1:746141/1‑148 (MQ=255)
           tGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCg                                                                                                                                   >  2:29951/1‑148 (MQ=255)
             ccGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTg                                                                                                                                 <  1:1290891/148‑1 (MQ=255)
             ccGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTg                                                                                                                                 >  1:1959426/1‑148 (MQ=255)
              cGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTgg                                                                                                                                <  1:1618050/148‑1 (MQ=255)
                gggTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATATCGAACAACAAGGTCGGTTGTTTTGCTTCATCGAAACAGGGCGTGGCa                                                                                                                              >  1:296385/1‑148 (MQ=255)
                 ggTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAt                                                                                                                             <  1:259382/148‑1 (MQ=255)
                  gTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCTTGGCAtt                                                                                                                            <  2:1444340/148‑1 (MQ=255)
                  gTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAtt                                                                                                                            <  2:1869908/148‑1 (MQ=255)
                   tATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTg                                                                                                                           <  1:1018131/148‑1 (MQ=255)
                      tGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCg                                                                                                                        >  1:2408291/1‑148 (MQ=255)
                              ccTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtg                                                                                                                >  1:1585138/1‑148 (MQ=255)
                               ctctTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtgg                                                                                                               >  1:3476319/1‑148 (MQ=255)
                                tctTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtggt                                                                                                              <  1:2347080/148‑1 (MQ=255)
                                 ctTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtggtg                                                                                                             >  1:1223501/1‑148 (MQ=255)
                                   ttGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGc                                                                                                           <  1:1542613/148‑1 (MQ=255)
                                      tCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCAtt                                                                                                        <  2:3240152/148‑1 (MQ=255)
                                        tGGGCGCTATTGGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCAtttt                                                                                                      <  2:1437388/148‑1 (MQ=255)
                                         gggCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGATACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTc                                                                                                     <  1:294658/148‑1 (MQ=255)
                                              cTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGt                                                                                                >  1:2032735/1‑148 (MQ=255)
                                                aTTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTg                                                                                              <  1:1860847/148‑1 (MQ=255)
                                                aTTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTg                                                                                              <  2:3061068/148‑1 (MQ=255)
                                                 tttGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTgg                                                                                             >  2:1365973/1‑148 (MQ=255)
                                                 tttGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTgg                                                                                             <  1:1519699/148‑1 (MQ=255)
                                                  ttGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGc                                                                                            >  1:2868754/1‑148 (MQ=255)
                                                  ttGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGc                                                                                            <  2:1267662/148‑1 (MQ=255)
                                                      cGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTa                                                                                        <  2:2668190/148‑1 (MQ=255)
                                                       gTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAt                                                                                       <  1:2731981/148‑1 (MQ=255)
                                                        ttACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAtt                                                                                      >  1:358601/1‑148 (MQ=255)
                                                        ttACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAtt                                                                                      <  2:1116795/148‑1 (MQ=255)
                                                        ttACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAtt                                                                                      >  2:263473/1‑148 (MQ=255)
                                                         tACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAttt                                                                                     >  2:876819/1‑148 (MQ=255)
                                                         tACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAttt                                                                                     <  1:1065117/148‑1 (MQ=255)
                                                         tACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAttt                                                                                     <  1:1278523/148‑1 (MQ=255)
                                                            ttGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTAcc                                                                                  <  1:1732472/148‑1 (MQ=255)
                                                            ttGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTAcc                                                                                  >  2:2656194/1‑148 (MQ=255)
                                                                gACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGt                                                                              <  1:2158004/148‑1 (MQ=255)
                                                                  cATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGttt                                                                            <  2:214704/148‑1 (MQ=255)
                                                                  cATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGttt                                                                            >  1:2440010/1‑148 (MQ=255)
                                                                        gaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGt                                                                      <  1:2719907/148‑1 (MQ=255)
                                                                          aCTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGTCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTTGTGGCATTTTCTGCGTTGGCAGTATTTAACTGGTTTTGCAGTgg                                                                    >  1:666403/1‑148 (MQ=255)
                                                                           cTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGc                                                                   <  2:541732/148‑1 (MQ=255)
                                                                           cTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGc                                                                   <  2:211862/148‑1 (MQ=255)
                                                                            tGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCt                                                                  <  2:3337407/148‑1 (MQ=255)
                                                                            tGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCt                                                                  <  2:2895995/148‑1 (MQ=255)
                                                                             ggCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtt                                                                 <  2:148443/148‑1 (MQ=255)
                                                                             ggCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtt                                                                 <  2:1688806/148‑1 (MQ=255)
                                                                             ggCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtt                                                                 >  1:3219434/1‑148 (MQ=255)
                                                                              gCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtta                                                                <  2:1081481/148‑1 (MQ=255)
                                                                              gCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtta                                                                <  2:33494/148‑1 (MQ=255)
                                                                                  gTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCGTATTAg                                                            >  2:3024906/1‑148 (MQ=255)
                                                                                   tCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCCGTATTTACCTGGTTTGGCCGTGGCTTATTggg                                                           >  1:5254/1‑148 (MQ=255)
                                                                                   tCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAgg                                                           >  1:1159495/1‑148 (MQ=255)
                                                                                    cTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGt                                                          >  2:2092066/1‑148 (MQ=255)
                                                                                    cTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGt                                                          >  1:1944534/1‑148 (MQ=255)
                                                                                      tGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGttt                                                        >  2:1508698/1‑148 (MQ=255)
                                                                                        cTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTa                                                      <  2:2550628/148‑1 (MQ=255)
                                                                                              tCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAg                                                >  1:1694968/1‑148 (MQ=255)
                                                                                                ggTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGc                                              <  1:1383368/148‑1 (MQ=255)
                                                                                                 gTAAGTAGATTGGGCAATAGCGAACAACAAGTTCGGTTGTTTGGCTTAATCGAAAAAGGGCGTGGCAATGTCGATACCGTGGTGGCATTTTCTGCGTATGCAGTATTTACAAGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCa                                             >  2:469110/1‑148 (MQ=255)
                                                                                                  tAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCAt                                            <  1:2889572/148‑1 (MQ=255)
                                                                                                       cGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGt                                       <  2:509891/148‑1 (MQ=255)
                                                                                                        gATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGtt                                      <  2:2709439/148‑1 (MQ=255)
                                                                                                         aTTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTc                                     <  2:1622038/148‑1 (MQ=255)
                                                                                                          ttGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCt                                    >  2:2446108/1‑148 (MQ=255)
                                                                                                            gggCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTCTTAGGTTTTAAAGCAGGCATCTGGTTCTAt                                  >  2:2854636/1‑148 (MQ=255)
                                                                                                             ggCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTAtt                                 <  2:2260407/148‑1 (MQ=255)
                                                                                                               cAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTcc                               >  1:2491163/1‑148 (MQ=255)
                                                                                                                aaTAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTccc                              <  1:589928/148‑1 (MQ=255)
                                                                                                                  tAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCtt                            >  1:3368622/1‑148 (MQ=255)
                                                                                                                  tAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCtt                            >  1:941029/1‑148 (MQ=255)
                                                                                                                   aGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTa                           <  2:564953/148‑1 (MQ=255)
                                                                                                                     cGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTAtt                         >  1:2516923/1‑148 (MQ=255)
                                                                                                                     cGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGGTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTAtt                         >  2:2659349/1‑148 (MQ=255)
                                                                                                                      gAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATtg                        >  2:2296425/1‑148 (MQ=255)
                                                                                                                        acaacaAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATtgtg                      <  1:263473/148‑1 (MQ=255)
                                                                                                                         caacaaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGa                     <  2:129771/148‑1 (MQ=255)
                                                                                                                            caaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTg                  <  2:790644/148‑1 (MQ=255)
                                                                                                                            caaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCGTATTGTGATTg                  >  2:2226457/1‑148 (MQ=255)
                                                                                                                             aaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGc                 >  2:1330407/1‑148 (MQ=255)
                                                                                                                             aaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGc                 >  1:2101284/1‑148 (MQ=255)
                                                                                                                              aGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGcc                >  2:47896/1‑148 (MQ=255)
                                                                                                                               ggTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCg               <  2:1799531/148‑1 (MQ=255)
                                                                                                                               ggTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCg               >  2:1303295/1‑148 (MQ=255)
                                                                                                                               ggTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATATACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCg               >  2:1177042/1‑148 (MQ=255)
                                                                                                                                 tCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAACGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTa             <  1:3256397/148‑1 (MQ=255)
                                                                                                                                      tgttTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCat        <  1:3488016/148‑1 (MQ=255)
                                                                                                                                       gttTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCatt       <  2:740334/148‑1 (MQ=255)
                                                                                                                                        tttGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCatta      <  1:876819/148‑1 (MQ=255)
                                                                                                                                          tGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattatt    <  2:358601/148‑1 (MQ=255)
                                                                                                                                          tGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattatt    <  2:545361/148‑1 (MQ=255)
                                                                                                                                           ggCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattatta   <  2:3260298/148‑1 (MQ=255)
                                                                                                                                            gCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattattat  <  2:296385/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTAT  >  CP009273/4055873‑4056160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTT  >  CP009273/4055871‑4056166
                                                                                                                                                   |                                                                                                                                                    
TATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATC                                                                                                                                                    <  SRR19973065.2170326/150‑1 (MQ=60)
TATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATC                                                                                                                                                    <  SRR19973065.3110218/150‑1 (MQ=60)
TATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATC                                                                                                                                                    <  SRR19973065.3110272/150‑1 (MQ=60)
TATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATC                                                                                                                                                    >  SRR19973065.335893/1‑150 (MQ=60)
     GACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAAC                                                                                                                                               <  SRR19973065.2557490/150‑1 (MQ=60)
       CGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAG                                                                                                                                             >  SRR19973065.3036024/1‑150 (MQ=60)
          CAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGC                                                                                                                                          <  SRR19973065.2980094/150‑1 (MQ=60)
          CAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGC                                                                                                                                          <  SRR19973065.2062516/150‑1 (MQ=60)
          CAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGC                                                                                                                                          >  SRR19973065.2274083/1‑150 (MQ=60)
           AATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCG                                                                                                                                         >  SRR19973065.751134/1‑150 (MQ=60)
             TGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTG                                                                                                                                       >  SRR19973065.1972225/1‑150 (MQ=60)
               CCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGC                                                                                                                                     <  SRR19973065.1299399/150‑1 (MQ=60)
                CGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATATCGAACAACAAGGTCGGTTGTTTTGCTTCATCGAAACAGGGCGTGGCA                                                                                                                                    >  SRR19973065.298290/1‑150 (MQ=60)
                CGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCA                                                                                                                                    <  SRR19973065.1628905/150‑1 (MQ=60)
                  GGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATT                                                                                                                                  <  SRR19973065.1968725/150‑1 (MQ=60)
                   GGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTG                                                                                                                                 <  SRR19973065.261176/150‑1 (MQ=60)
                     TATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTC                                                                                                                               <  SRR19973065.1024879/150‑1 (MQ=60)
                      ATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCG                                                                                                                              >  SRR19973065.2423450/1‑150 (MQ=60)
                        TGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGAT                                                                                                                            <  SRR19973065.665759/150‑1 (MQ=60)
                              ATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTG                                                                                                                      >  SRR19973065.1595710/1‑150 (MQ=60)
                              ATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGAG                                                                                                                      >  SRR19973065.1594653/1‑150 (MQ=60)
                               TCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGG                                                                                                                     >  SRR19973065.3497470/1‑150 (MQ=60)
                                 CTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTG                                                                                                                   >  SRR19973065.1231472/1‑150 (MQ=60)
                                  TCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGG                                                                                                                  <  SRR19973065.2361712/150‑1 (MQ=60)
                                  TCTTTGTCTGGGCGCGATTGGGCGTTACTTGCGACATGATGAACGGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGG                                                                                                                  <  SRR19973065.675202/150‑1 (MQ=60)
                                   CTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGC                                                                                                                 >  SRR19973065.2364444/1‑150 (MQ=60)
                                   CTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGC                                                                                                                 >  SRR19973065.2145396/1‑150 (MQ=60)
                                     TTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCAT                                                                                                               <  SRR19973065.1552750/150‑1 (MQ=60)
                                     TTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCAT                                                                                                               <  SRR19973065.1552813/150‑1 (MQ=60)
                                           GGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGATACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTG                                                                                                         <  SRR19973065.296554/150‑1 (MQ=60)
                                             GCGCTATTTGGCGTTTCTTGCGACATGATGAACTTGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGTCAATAGCGAACAACAAGGTCTGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCG                                                                                                       <  SRR19973065.1224272/150‑1 (MQ=60)
                                              CGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGT                                                                                                      >  SRR19973065.2045779/1‑150 (MQ=60)
                                               GCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTT                                                                                                     <  SRR19973065.2472510/150‑1 (MQ=60)
                                                  ATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGC                                                                                                  <  SRR19973065.1873095/150‑1 (MQ=60)
                                                  ATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGC                                                                                                  >  SRR19973065.2886349/1‑150 (MQ=60)
                                                   TTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCA                                                                                                 <  SRR19973065.1529651/150‑1 (MQ=60)
                                                        CGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATT                                                                                            >  SRR19973065.361125/1‑150 (MQ=60)
                                                         GTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTT                                                                                           <  SRR19973065.2748978/150‑1 (MQ=60)
                                                           TACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTAC                                                                                         <  SRR19973065.1072170/150‑1 (MQ=60)
                                                           TACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTAC                                                                                         <  SRR19973065.1286964/150‑1 (MQ=60)
                                                           TACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTAC                                                                                         <  SRR19973065.2801213/150‑1 (MQ=60)
                                                           TACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTAC                                                                                         <  SRR19973065.2746160/150‑1 (MQ=60)
                                                              TTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTG                                                                                      <  SRR19973065.1743937/150‑1 (MQ=60)
                                                                  GACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTTTGTCGATTGGGCAATAGCGTACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTT                                                                                  <  SRR19973065.1729415/150‑1 (MQ=60)
                                                                  GACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTT                                                                                  <  SRR19973065.2171878/150‑1 (MQ=60)
                                                                  GACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTT                                                                                  <  SRR19973065.1486792/150‑1 (MQ=60)
                                                                  GACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTT                                                                                  <  SRR19973065.393382/150‑1 (MQ=60)
                                                                  GACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTT                                                                                  <  SRR19973065.188228/150‑1 (MQ=60)
                                                                  GACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTT                                                                                  >  SRR19973065.2455392/1‑150 (MQ=60)
                                                                  GACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTT                                                                                  <  SRR19973065.2171984/150‑1 (MQ=60)
                                                                   ACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTG                                                                                 <  SRR19973065.3180068/150‑1 (MQ=60)
                                                                    CATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGG                                                                                <  SRR19973065.1314406/150‑1 (MQ=60)
                                                                       GATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAG                                                                             <  SRR19973065.779507/150‑1 (MQ=60)
                                                                          GAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGTCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTTGTGGCATTTTCTGCGTTGGCAGTATTTAACTGGTTTTGCAGTGG                                                                          >  SRR19973065.670839/1‑150 (MQ=60)
                                                                          GAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGG                                                                          <  SRR19973065.2736824/150‑1 (MQ=60)
                                                                          GAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGG                                                                          <  SRR19973065.200577/150‑1 (MQ=60)
                                                                             CTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTT                                                                       >  SRR19973065.3238945/1‑150 (MQ=60)
                                                                             CTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTT                                                                       >  SRR19973065.3238786/1‑150 (MQ=60)
                                                                                 CCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTA                                                                   <  SRR19973065.243674/150‑1 (MQ=60)
                                                                                   GGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCCGTATTTACCTGGTTTGGCCGTGGCTTATTGGG                                                                 >  SRR19973065.5333/1‑150 (MQ=60)
                                                                                   GGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGG                                                                 >  SRR19973065.1166923/1‑150 (MQ=60)
                                                                                   GGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGG                                                                 >  SRR19973065.2338823/1‑150 (MQ=60)
                                                                                    GTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGT                                                                >  SRR19973065.1957245/1‑150 (MQ=60)
                                                                                         GCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAA                                                           <  SRR19973065.1065165/150‑1 (MQ=60)
                                                                                            CAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGC                                                        >  SRR19973065.3180080/1‑150 (MQ=60)
                                                                                             AAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCA                                                       >  SRR19973065.1824109/1‑150 (MQ=60)
                                                                                              AGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAG                                                      <  SRR19973065.2515263/150‑1 (MQ=60)
                                                                                              AGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAG                                                      >  SRR19973065.1706281/1‑150 (MQ=60)
                                                                                                  GGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCAT                                                  >  SRR19973065.128010/1‑150 (MQ=60)
                                                                                                  GGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCAT                                                  <  SRR19973065.1392471/150‑1 (MQ=60)
                                                                                                    TAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCT                                                <  SRR19973065.2907232/150‑1 (MQ=60)
                                                                                                     AAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTG                                               >  SRR19973065.534907/1‑150 (MQ=60)
                                                                                                          GATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCT                                          >  SRR19973065.290621/1‑150 (MQ=60)
                                                                                                            TTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTAT                                        <  SRR19973065.3500631/150‑1 (MQ=60)
                                                                                                             TGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATT                                       <  SRR19973065.3416690/150‑1 (MQ=60)
                                                                                                               GGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCC                                     >  SRR19973065.1698406/1‑150 (MQ=60)
                                                                                                               GGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCC                                     >  SRR19973065.2506843/1‑150 (MQ=60)
                                                                                                                  AATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTT                                  >  SRR19973065.3388803/1‑150 (MQ=60)
                                                                                                                  AATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTT                                  <  SRR19973065.3519050/150‑1 (MQ=60)
                                                                                                                  AATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTT                                  <  SRR19973065.593586/150‑1 (MQ=60)
                                                                                                                  AATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTT                                  >  SRR19973065.3389223/1‑150 (MQ=60)
                                                                                                                  AATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTT                                  >  SRR19973065.947242/1‑150 (MQ=60)
                                                                                                                     AGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATT                               >  SRR19973065.2532734/1‑150 (MQ=60)
                                                                                                                        GAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTG                            <  SRR19973065.2054278/150‑1 (MQ=60)
                                                                                                                        GAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTAACTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATATTGTTCTATTCACTTATTGTG                            >  SRR19973065.2730355/1‑150 (MQ=60)
                                                                                                                          ACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGAT                          <  SRR19973065.265280/150‑1 (MQ=60)
                                                                                                                             ACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGC                       >  SRR19973065.2114797/1‑150 (MQ=60)
                                                                                                                              CAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCC                      >  SRR19973065.2648145/1‑150 (MQ=60)
                                                                                                                               AAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTAACTGGTTTGGCAGTGGCGTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCGTATTGTGATTGCCG                     >  SRR19973065.2918114/1‑150 (MQ=60)
                                                                                                                                AGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTACCTTATTGTGATTGCCGT                    >  SRR19973065.1292654/1‑150 (MQ=60)
                                                                                                                                   TCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAACGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGG                 <  SRR19973065.3276096/150‑1 (MQ=60)
                                                                                                                                   TCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGG                 <  SRR19973065.3276918/150‑1 (MQ=60)
                                                                                                                                      GTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCAT              >  SRR19973065.1601792/1‑150 (MQ=60)
                                                                                                                                      GTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCAT              <  SRR19973065.30334/150‑1 (MQ=60)
                                                                                                                                       TTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATT             <  SRR19973065.1140833/150‑1 (MQ=60)
                                                                                                                                        TGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTA            <  SRR19973065.3509228/150‑1 (MQ=60)
                                                                                                                                          TTTGGCTTCATCGAATCAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATT          <  SRR19973065.882416/150‑1 (MQ=60)
                                                                                                                                          TTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATT          <  SRR19973065.882429/150‑1 (MQ=60)
                                                                                                                                                  CATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCGTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTCGGCATTATTATTTTCTT  >  SRR19973065.618709/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
TATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTTCTT  >  CP009273/4055871‑4056166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑