Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F52 I0 R1 779 320.1 10410758 96.6% 10056792 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,556,774 G→A 21.5% intergenic (+298/‑177) yffP → / → yffQ CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,556,7740GA21.5% 784.2 / 200.7 330intergenic (+298/‑177)yffP/yffQCPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (117/142);  new base A (33/38);  total (150/180)
Fisher's exact test for biased strand distribution p-value = 8.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 338 total aligned reads displayed.

ACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTT  >  CP009273/2556626‑2556922
                                                                                                                                                      |                                                                                                                                                     
  cTACCCGGGCCTTTCTCACTATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGt                                                                                                                                                     <  2:4182691/149‑1 (MQ=255)
  cTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGt                                                                                                                                                     <  2:3561847/149‑1 (MQ=255)
     cccGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGaa                                                                                                                                                  <  1:2343713/149‑1 (MQ=255)
      ccGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAAt                                                                                                                                                 <  1:301868/149‑1 (MQ=255)
       cGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAAtt                                                                                                                                                <  1:1126994/149‑1 (MQ=255)
        gggCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAAttt                                                                                                                                               <  2:3323531/149‑1 (MQ=255)
         ggCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAAtttt                                                                                                                                              <  1:2822432/149‑1 (MQ=255)
         ggCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAAtttt                                                                                                                                              >  1:2183129/1‑149 (MQ=255)
         ggCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAAtttt                                                                                                                                              >  1:3539802/1‑149 (MQ=255)
             tttCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGATCATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGa                                                                                                                                          <  2:2458356/149‑1 (MQ=255)
             tttCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGa                                                                                                                                          >  1:3725562/1‑149 (MQ=255)
               tctcACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATg                                                                                                                                        >  2:4657448/1‑149 (MQ=255)
                 tcACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCa                                                                                                                                      <  1:1622855/149‑1 (MQ=255)
                    caATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGAt                                                                                                                                   <  2:305999/149‑1 (MQ=255)
                     aaTCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATc                                                                                                                                  >  2:1739670/1‑149 (MQ=255)
                        ccccGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCt                                                                                                                               <  1:3147123/149‑1 (MQ=255)
                         cccGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCtt                                                                                                                              <  1:4482724/149‑1 (MQ=255)
                         cccGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCtt                                                                                                                              >  2:1168130/1‑149 (MQ=255)
                               gACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGTCTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTccc                                                                                                                        <  1:2778149/149‑1 (MQ=255)
                               gACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTccc                                                                                                                        <  2:3884042/149‑1 (MQ=255)
                                    tataTCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAg                                                                                                                   <  1:74613/149‑1 (MQ=255)
                                     atatCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAgg                                                                                                                  >  1:4807986/1‑149 (MQ=255)
                                      tatCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGc                                                                                                                 >  2:1316419/1‑149 (MQ=255)
                                        tCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCAt                                                                                                               >  1:1551457/1‑149 (MQ=255)
                                          aCCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCAttt                                                                                                             <  1:4245569/149‑1 (MQ=255)
                                           cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCAtttt                                                                                                            >  1:1380076/1‑149 (MQ=255)
                                           cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCAtttt                                                                                                            <  1:4135475/149‑1 (MQ=255)
                                           cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCAtttt                                                                                                            >  1:3400856/1‑149 (MQ=255)
                                           cccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCAtttt                                                                                                            <  2:2422262/149‑1 (MQ=255)
                                            ccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCAttttt                                                                                                           <  1:64139/149‑1 (MQ=255)
                                            ccATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCAttttt                                                                                                           <  1:2569822/149‑1 (MQ=255)
                                              aTCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGt                                                                                                         >  1:1725538/1‑149 (MQ=255)
                                                cTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGttt                                                                                                       <  1:3093124/149‑1 (MQ=255)
                                                cTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGttt                                                                                                       <  2:2097843/149‑1 (MQ=255)
                                                 tttCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTa                                                                                                      <  1:3669823/149‑1 (MQ=255)
                                                  ttCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTaa                                                                                                     >  2:2208056/1‑149 (MQ=255)
                                                    ccGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAAtt                                                                                                   <  2:1352007/149‑1 (MQ=255)
                                                      gTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtca                                                                                                 >  2:4704750/1‑149 (MQ=255)
                                                      gTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtca                                                                                                 >  1:4972665/1‑149 (MQ=255)
                                                       ttttCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATtcaa                                                                                                >  2:579863/1‑149 (MQ=255)
                                                            aGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAAc                                                                                           <  2:2698419/149‑1 (MQ=255)
                                                                 ggAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAg                                                                                      <  2:3392041/149‑1 (MQ=255)
                                                                  gagaCTGATGGCCGCCGACGTTGAACAATTGATTAATTTATGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAgg                                                                                     <  1:5171317/149‑1 (MQ=255)
                                                                   agaCTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGc                                                                                    <  1:1805688/149‑1 (MQ=255)
                                                                   agaCTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACAGTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGc                                                                                    <  2:761680/149‑1 (MQ=255)
                                                                      cTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGt                                                                                 <  2:1745582/149‑1 (MQ=255)
                                                                        gATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTc                                                                               >  1:3834503/1‑149 (MQ=255)
                                                                          tGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTccc                                                                             <  1:194846/149‑1 (MQ=255)
                                                                                ccgACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCcaacaac                                                                       <  1:828287/149‑1 (MQ=255)
                                                                                ccgACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCcaacaac                                                                       <  2:3967273/149‑1 (MQ=255)
                                                                                 cgACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCcaacaaca                                                                      >  2:3202479/1‑149 (MQ=255)
                                                                                   aCGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACaaa                                                                    <  2:4058030/149‑1 (MQ=255)
                                                                                   aCGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACaaa                                                                    <  2:5001576/149‑1 (MQ=255)
                                                                                    cGTTGCACAATTGATTAATTTCTGAACATTCAAAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACaaa                                                                    <  2:3789910/149‑1 (MQ=255)
                                                                                    cGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACaaaa                                                                   <  1:4540174/149‑1 (MQ=255)
                                                                                     gTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAAt                                                                  <  1:4281675/149‑1 (MQ=255)
                                                                                        gCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATaaa                                                               >  2:3475196/1‑149 (MQ=255)
                                                                                          acaATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGa                                                             >  1:4044687/1‑149 (MQ=255)
                                                                                          acaATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGCTGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGa                                                             <  2:5156285/149‑1 (MQ=255)
                                                                                            aaTTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATg                                                           >  1:4597927/1‑149 (MQ=255)
                                                                                             aTTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATgg                                                          >  2:2556667/1‑149 (MQ=255)
                                                                                             aTTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATgg                                                          >  2:2726308/1‑149 (MQ=255)
                                                                                                       ttCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaa                                                <  1:3516330/149‑1 (MQ=255)
                                                                                                       ttCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTaaaaa                                                <  2:16409/149‑1 (MQ=255)
                                                                                                         cTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATg                                              <  2:2881720/149‑1 (MQ=255)
                                                                                                          tGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑a                                            >  2:3757237/1‑149 (MQ=255)
                                                                                                          tGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACAAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑a                                            <  1:114767/149‑1 (MQ=255)
                                                                                                          agAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑a                                            >  1:5120108/2‑149 (MQ=255)
                                                                                                            aaCATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑aaa                                          >  1:4914717/1‑149 (MQ=255)
                                                                                                            aaCATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑aaa                                          >  2:4857358/1‑149 (MQ=255)
                                                                                                            aaCATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑aaa                                          >  1:2920042/1‑149 (MQ=255)
                                                                                                               aTTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGGAAGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACg                                        >  2:4761181/1‑149 (MQ=255)
                                                                                                                ttC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGt                                      <  1:1454849/149‑1 (MQ=255)
                                                                                                                ttC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGAAGTTCACAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGt                                      >  2:3934920/1‑149 (MQ=255)
                                                                                                                  c‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTgg                                    <  2:1763991/149‑1 (MQ=255)
                                                                                                                  c‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTgg                                    >  1:330122/1‑149 (MQ=255)
                                                                                                                    aaaTCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTggg                                   >  2:4390029/1‑149 (MQ=255)
                                                                                                                    aaaTCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTggg                                   >  2:1271537/1‑149 (MQ=255)
                                                                                                                    aaaTCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTggg                                   <  1:1717956/149‑1 (MQ=255)
                                                                                                                     aaTCAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGa                                  >  2:1942970/1‑149 (MQ=255)
                                                                                                                      aTCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGaa                                 <  2:151192/149‑1 (MQ=255)
                                                                                                                        cAACTCCCACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGt                               <  2:1900016/149‑1 (MQ=255)
                                                                                                                             cccACCAAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTTAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCtt                          >  2:3722144/1‑149 (MQ=255)
                                                                                                                              ccaccaAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCttt                         >  2:4260632/1‑149 (MQ=255)
                                                                                                                              ccaccaAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCttt                         <  1:551607/149‑1 (MQ=255)
                                                                                                                               caccaAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTa                        >  1:885802/1‑149 (MQ=255)
                                                                                                                                accaAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTAc                       >  1:5055420/1‑149 (MQ=255)
                                                                                                                                accaAAGCCAACAAGATGGG‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTAc                       >  1:1236809/1‑149 (MQ=255)
                                                                                                                                    aaGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAAACGGTGGGAAGTGGCTTTACTgg                    <  1:579863/149‑1 (MQ=255)
                                                                                                                                    aaGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGt                   >  1:1067470/1‑149 (MQ=255)
                                                                                                                                    aaGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGt                   >  1:462065/1‑149 (MQ=255)
                                                                                                                                     aGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGTg                  >  1:4386032/1‑149 (MQ=255)
                                                                                                                                     aGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGTg                  >  1:2690528/1‑149 (MQ=255)
                                                                                                                                        caacaaGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATGAAAACGGTGGGAAGTGGCTTTACTGGTGAt                >  1:3949544/1‑149 (MQ=255)
                                                                                                                                          acaaGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGTGATGGt             >  2:79653/1‑149 (MQ=255)
                                                                                                                                                tGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACACAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGtt       >  2:48091/1‑149 (MQ=255)
                                                                                                                                                tGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGtt       <  2:3418883/149‑1 (MQ=255)
                                                                                                                                                 ggg‑AAGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTg      >  2:1010091/1‑149 (MQ=255)
                                                                                                                                                     aGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGtt   <  2:1991538/149‑1 (MQ=255)
                                                                                                                                                      ggTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTGGGttt  >  2:5066213/1‑149 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ACCTACCCGGGCCTTTCTCACAATCCCCGCTGACATTATATCACCCATCTTTCCGTTTTCAGGTGGGAGACTGATGGCCGCCGACGTTGCACAATTGATTAATTTCTGAACATTC‑AAATCAACTCCCACCAAAGCCAACAAGATGGG‑AGGTGAATTTTGTGATGCAGATCGCTTTTTCCCTTCAGGCATTTTTGTTTAATTCAATCAACGAAAGGCAGTTCCCAACAACAAAATAAAGATGGGAGGTAAAAATG‑AAACGGTGGGAAGTGGCTTTACTGGTGATGGTAATGTTGGTTT  >  CP009273/2556626‑2556922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A