Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 437,672 T→A 100% V223E (GTG→GAG)  thiI → tRNA s(4)U8 sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273437,6720TA99.4% 538.4 / ‑4.5 170V223E (GTG→GAG) thiItRNA s(4)U8 sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (75/94);  total (75/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 171 total aligned reads displayed.

GGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTG  >  CP009273/437527‑437815
                                                                                                                                                 |                                                                                                                                               
 gCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGGTTCGACTCCGGGGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGgc                                                                                                                                              >  1:794675/1‑148 (MQ=255)
   aCCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGgcgc                                                                                                                                            <  2:2774430/148‑1 (MQ=255)
    cccAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGGTATATGTTGAAGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCa                                                                                                                                           >  1:1350958/1‑148 (MQ=255)
     ccAGGAAGATGTGCTGTCGCTGATTTACGGTGGTTTAGACTCCGGGGTTTGCCGGTATATTTTGATGCGTCGCGGCGGCCGCGTGCATTAAAGCTTCTTTAACCTCGGCGGCGCGGCGCCTGAAATTTGCGTGCGGCAGGAGgcgcct                                                                                                                                          >  1:19855/1‑146 (MQ=255)
        ggAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTAt                                                                                                                                       <  1:1540249/148‑1 (MQ=255)
         gAAGATGTGCTGTCGCTCATTTCCGGTGGTTTGGACTCCGGTGTTTGCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCAATAATGCTTATTTAAACTCGGCGGCGCGGCGCATGAAAATGGCGTGCGTCAGGAGGCGCATTATc                                                                                                                                      >  1:385993/1‑148 (MQ=255)
            gATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCtgt                                                                                                                                   <  1:165055/148‑1 (MQ=255)
            gATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCtgt                                                                                                                                   <  1:3075729/148‑1 (MQ=255)
                tgCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATTCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATATGTGGaa                                                                                                                               >  1:3006239/1‑148 (MQ=255)
                     tCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCt                                                                                                                          <  1:610353/148‑1 (MQ=255)
                      cGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCtt                                                                                                                         <  2:1546685/148‑1 (MQ=255)
                      cGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCtt                                                                                                                         <  2:1579834/148‑1 (MQ=255)
                        cTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTg                                                                                                                       >  2:1356695/1‑148 (MQ=255)
                         tCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTgg                                                                                                                      >  1:892030/1‑148 (MQ=255)
                         tCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTgg                                                                                                                      <  2:237512/148‑1 (MQ=255)
                          cATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGc                                                                                                                     >  2:1326091/1‑148 (MQ=255)
                           aTTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCa                                                                                                                    >  2:2073129/1‑148 (MQ=255)
                               ccGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTc                                                                                                                <  1:1299218/148‑1 (MQ=255)
                                 ggtgttTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTccc                                                                                                              >  1:1301443/1‑148 (MQ=255)
                                  gtggtTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCa                                                                                                             >  2:754454/1‑148 (MQ=255)
                                         cGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCgcgtg                                                                                                      <  2:2677904/148‑1 (MQ=255)
                                            cTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCgcgtgcgt                                                                                                   <  1:1452539/148‑1 (MQ=255)
                                              ccGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGttt                                                                                                 <  1:2073129/148‑1 (MQ=255)
                                               cGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGtttt                                                                                                <  2:2537070/148‑1 (MQ=255)
                                               cGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGtttt                                                                                                >  1:1546201/1‑148 (MQ=255)
                                                ggTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTg                                                                                               >  2:1957064/1‑148 (MQ=255)
                                                 gtgtTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGt                                                                                              >  1:2507528/1‑148 (MQ=255)
                                                 gtgtTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGt                                                                                              >  1:1851821/1‑148 (MQ=255)
                                                  tgtTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTc                                                                                             <  2:531484/148‑1 (MQ=255)
                                                     ttCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCt                                                                                          <  2:31478/148‑1 (MQ=255)
                                                     ttCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCt                                                                                          <  1:3128165/148‑1 (MQ=255)
                                                     ttCCAGTTATATGTGGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCt                                                                                          <  2:1709974/148‑1 (MQ=255)
                                                       ccAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTAt                                                                                        <  2:858255/148‑1 (MQ=255)
                                                          gTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTaa                                                                                     <  2:366361/148‑1 (MQ=255)
                                                          gTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTaa                                                                                     <  2:366357/148‑1 (MQ=255)
                                                          gTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTaa                                                                                     <  2:3278063/148‑1 (MQ=255)
                                                           ttATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGGGCATGAATTTGGGGTGCGTCAGGAGGCGCATTATCGGTGGAACCGCTTTGGCAGCTCCAACCGCGTGCGTTTTGTCGCTATTAAt                                                                                    <  2:2808977/148‑1 (MQ=255)
                                                           ttATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAt                                                                                    <  1:2585252/148‑1 (MQ=255)
                                                             atatGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAttt                                                                                  >  2:216696/1‑148 (MQ=255)
                                                             atatGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAttt                                                                                  <  2:2431987/148‑1 (MQ=255)
                                                               atGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCAACCGCGTGCGTTTTGTCGCTATTAATTTCg                                                                                >  2:826498/1‑148 (MQ=255)
                                                                 gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGaa                                                                              >  1:536492/1‑148 (MQ=255)
                                                                 gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGaa                                                                              >  1:2337910/1‑148 (MQ=255)
                                                                 gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGaa                                                                              >  1:114897/1‑148 (MQ=255)
                                                                     aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCgg                                                                          >  2:2204150/1‑148 (MQ=255)
                                                                      tGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGgt                                                                         <  2:2841442/148‑1 (MQ=255)
                                                                        cGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGgtcg                                                                       <  1:1929856/148‑1 (MQ=255)
                                                                         gTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGgtcgt                                                                      <  2:2283207/148‑1 (MQ=255)
                                                                         gTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGgtcgt                                                                      <  2:1488478/148‑1 (MQ=255)
                                                                            gcgGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCgg                                                                   <  2:3288074/148‑1 (MQ=255)
                                                                            gcgGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCgg                                                                   <  2:1163206/148‑1 (MQ=255)
                                                                              ggCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCgggg                                                                 <  1:2245954/148‑1 (MQ=255)
                                                                                ctcccGCGTGCAGTCCCGCTTCATAAACCTCGCCGGCGCGCCGCATGACCTTGGCGCTCGTAAGGACGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGaa                                                               <  2:2798672/145‑1 (MQ=255)
                                                                                    cgcgTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATtc                                                           <  2:566215/148‑1 (MQ=255)
                                                                                     gcgTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATtct                                                          <  2:2214872/148‑1 (MQ=255)
                                                                                     gcgTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATtct                                                          <  1:1575064/148‑1 (MQ=255)
                                                                                      cgTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATtctc                                                         >  2:768362/1‑148 (MQ=255)
                                                                                        tGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCga                                                       >  2:636978/1‑148 (MQ=255)
                                                                                        tGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCga                                                       >  1:3301766/1‑148 (MQ=255)
                                                                                        tGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCga                                                       >  1:1734571/1‑148 (MQ=255)
                                                                                         gCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGGACTTCTCgag                                                      >  1:1687410/1‑148 (MQ=255)
                                                                                         gCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCgag                                                      >  2:1632798/1‑148 (MQ=255)
                                                                                          cATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCgaga                                                     >  2:783462/1‑148 (MQ=255)
                                                                                            ttACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGaaa                                                   >  2:469060/1‑148 (MQ=255)
                                                                                             tACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGaaaa                                                  >  2:619456/1‑148 (MQ=255)
                                                                                                tGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGTCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcg                                               <  1:390209/148‑1 (MQ=255)
                                                                                                tGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcg                                               <  1:1022814/148‑1 (MQ=255)
                                                                                                 gCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcga                                              <  1:2120566/148‑1 (MQ=255)
                                                                                                 gCTTCTGTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcga                                              <  2:1407399/148‑1 (MQ=255)
                                                                                                     cttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCgacgac                                          <  2:3209446/148‑1 (MQ=255)
                                                                                                     cttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCgacgac                                          >  2:2933012/1‑148 (MQ=255)
                                                                                                      tttAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAATTTCTCGAGAAAATCGacgacg                                         <  2:2648134/148‑1 (MQ=255)
                                                                                                       ttAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACgg                                        >  1:1665165/1‑148 (MQ=255)
                                                                                                       ttAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACgg                                        >  1:571837/1‑148 (MQ=255)
                                                                                                         aaCCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGACCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTc                                      <  1:3279509/148‑1 (MQ=255)
                                                                                                          aCCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCCTGCGTGTTGTCGCTAGTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAACATCGACGACGGTCa                                     >  2:2865170/1‑148 (MQ=255)
                                                                                                            cTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGa                                   <  1:1021463/148‑1 (MQ=255)
                                                                                                             tCGGCGGCGCGGCGCATGAATTTGGCGTGCTTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGAt                                  <  2:271401/148‑1 (MQ=255)
                                                                                                             tCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGAt                                  >  1:2642185/1‑148 (MQ=255)
                                                                                                             tCGGCGGCGCGGCGAATGATTTTGGCGTGCGTCTGGAGGCGCATTATCTTTGGAACCGCTTTGTCAGCTCCCACCTCGTGAGTTTTGTCGCTATTATTTTCGAACCGGTCGTCGGGTAATTTCTCGAGAAAATCGACGACGGTCAGAt                                  <  2:2367531/148‑1 (MQ=255)
                                                                                                                gcggcgcggcGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATggg                               <  1:2654491/148‑1 (MQ=255)
                                                                                                                 cggcgcggcgCATGAATTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGc                              <  1:51382/148‑1 (MQ=255)
                                                                                                                  ggcgcggcgcATGAATTTGGCGTGCGTCAGGAGGCGCATTATCGGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCTTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCg                             <  1:2709413/148‑1 (MQ=255)
                                                                                                                    cgcggcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGtt                           <  1:755380/148‑1 (MQ=255)
                                                                                                                       ggcgcATTAAATTGGGGTGCGTCTGGAGGCGATTTATCTGTGGAACCGCTTTGGCAGGTACAACCGCGTGGGTTTTTTCGCTATTAATTTCGAACCGGTCTTCGGGGAAAGTCTGGAGAAAATCGACGACGGTCAGATGGGCGTTTTc                        <  1:1650138/148‑1 (MQ=255)
                                                                                                                        gcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGACCTTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATcc                       >  2:2501097/1‑148 (MQ=255)
                                                                                                                        gcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATcc                       >  1:2771290/1‑148 (MQ=255)
                                                                                                                        gcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATcc                       >  2:2501167/1‑148 (MQ=255)
                                                                                                                             tGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaaa                  <  1:1356731/148‑1 (MQ=255)
                                                                                                                              gAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAAc                 >  1:712593/1‑148 (MQ=255)
                                                                                                                               aaaTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACg                >  1:3234174/1‑148 (MQ=255)
                                                                                                                               aaaTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACg                >  1:1596252/1‑148 (MQ=255)
                                                                                                                                 ttgggCGTGCGTCCGGAGGCGCATTATCTGTGGAACCGATTTGGCAGCTCCCACCGGGTGCTTTTTGTCGATATTAATTTCGAACGGGTGGTCGGGGAAATTCTCGACAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTa              <  1:560313/145‑1 (MQ=255)
                                                                                                                                  ttGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTat             <  2:1749405/148‑1 (MQ=255)
                                                                                                                                   tGGCGTGCGTCAGGAGGCGCATTATCTGTGGACCCGCTGTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatg            <  2:2698280/148‑1 (MQ=255)
                                                                                                                                   tGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatg            >  1:241859/1‑148 (MQ=255)
                                                                                                                                   tGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatg            >  1:405111/1‑148 (MQ=255)
                                                                                                                                     gcgtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatgat          >  1:892529/1‑148 (MQ=255)
                                                                                                                                      cgtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatgatg         <  2:488205/148‑1 (MQ=255)
                                                                                                                                      cgtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatgatg         <  2:488154/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTG  >  CP009273/437527‑437815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCAT  >  CP009273/437523‑437821
                                                                                                                                                     |                                                                                                                                                     
GATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGA                                                                                                                                                       <  SRR19973070.2540254/150‑1 (MQ=60)
 ATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAG                                                                                                                                                      <  SRR19973070.2564861/150‑1 (MQ=60)
 ATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAG                                                                                                                                                      <  SRR19973070.2564821/150‑1 (MQ=60)
   CGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGGTTCGACTCCGGGGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGC                                                                                                                                                    >  SRR19973070.800166/1‑150 (MQ=60)
      CACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGGTATATGTTGAAGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCA                                                                                                                                                 >  SRR19973070.1360210/1‑150 (MQ=60)
       ACCCAGGAAGATGTGCTGTCGCTGATTTACGGTGGTTTAGACTCCGGGGTTTGCCGGTATATTTTGATGCGTCGCGGCGGCCGCGTGCATTAAAGCTTCTTTAACCTCGGCGGCGCGGCGCCTGAAATTTGCGTGCGGCAGGAGGCGCCT                                                                                                                                                >  SRR19973070.20145/1‑150 (MQ=60)
        CCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATT                                                                                                                                               <  SRR19973070.311310/150‑1 (MQ=60)
        CCCAGGAAGATGTGCTGTCGCTCATTTCCGGGGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGGCAGGAGGCGCATT                                                                                                                                               >  SRR19973070.1761530/1‑150 (MQ=60)
          CAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTAT                                                                                                                                             <  SRR19973070.2001484/150‑1 (MQ=60)
           AGGAAGATGTGCTGTCGCTGATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGGTGATGCGTCGCGGCTGCCGCGTGCATTAATGCTTCTTTAACCTCGGCGGCGCGGCGCCTGAAATTGGCGTGCGTCAGGAGGCGCATTATA                                                                                                                                            >  SRR19973070.388795/1‑150 (MQ=60)
           AGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTGGACTCCGGTGTTTGCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCAATAATGCTTATTTAAACTCGGCGGCGCGGCGCATGAAAATGGCGTGCGTCAGGAGGCGCATTATC                                                                                                                                            >  SRR19973070.388825/1‑150 (MQ=60)
            GGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCT                                                                                                                                           <  SRR19973070.1551073/150‑1 (MQ=60)
            GGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCT                                                                                                                                           <  SRR19973070.1552358/150‑1 (MQ=60)
             GAAGACGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGTTTCTTTAACCTTGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTG                                                                                                                                          <  SRR19973070.568756/150‑1 (MQ=60)
                GATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGG                                                                                                                                       <  SRR19973070.166390/150‑1 (MQ=60)
                GATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGG                                                                                                                                       <  SRR19973070.166400/150‑1 (MQ=60)
                GATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGG                                                                                                                                       <  SRR19973070.406857/150‑1 (MQ=60)
                GATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGG                                                                                                                                       <  SRR19973070.3095483/150‑1 (MQ=60)
                  TGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATTCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATATGTGGAA                                                                                                                                     >  SRR19973070.3025447/1‑150 (MQ=60)
                         TCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTT                                                                                                                              <  SRR19973070.614634/150‑1 (MQ=60)
                         TCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTT                                                                                                                              <  SRR19973070.613807/150‑1 (MQ=60)
                           GCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGG                                                                                                                            >  SRR19973070.898328/1‑150 (MQ=60)
                                   CCGGTGTTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCC                                                                                                                    >  SRR19973070.1310491/1‑150 (MQ=60)
                                   CCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCC                                                                                                                    <  SRR19973070.1308258/150‑1 (MQ=60)
                                   CCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCC                                                                                                                    <  SRR19973070.1167172/150‑1 (MQ=60)
                                        GGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGC                                                                                                               <  SRR19973070.1037286/150‑1 (MQ=60)
                                             CGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCG                                                                                                          <  SRR19973070.1679454/150‑1 (MQ=60)
                                              GACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGT                                                                                                         >  SRR19973070.1505750/1‑150 (MQ=60)
                                                CTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTT                                                                                                       <  SRR19973070.1462646/150‑1 (MQ=60)
                                                 TCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTT                                                                                                      <  SRR19973070.1741069/150‑1 (MQ=60)
                                                 TCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTT                                                                                                      >  SRR19973070.1557076/1‑150 (MQ=60)
                                                  CCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTG                                                                                                     <  SRR19973070.2087086/150‑1 (MQ=60)
                                                   CGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGT                                                                                                    >  SRR19973070.2523828/1‑150 (MQ=60)
                                                   CGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGT                                                                                                    >  SRR19973070.1864509/1‑150 (MQ=60)
                                                     GTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCG                                                                                                  >  SRR19973070.3206865/1‑150 (MQ=60)
                                                     GTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCG                                                                                                  >  SRR19973070.2205476/1‑150 (MQ=60)
                                                         TTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTAT                                                                                              <  SRR19973070.3148364/150‑1 (MQ=60)
                                                               TTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTT                                                                                        <  SRR19973070.2602143/150‑1 (MQ=60)
                                                                TATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTC                                                                                       <  SRR19973070.2563360/150‑1 (MQ=60)
                                                                   ATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAA                                                                                    >  SRR19973070.115948/1‑150 (MQ=60)
                                                                   ATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAA                                                                                    >  SRR19973070.540090/1‑150 (MQ=60)
                                                                   ATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAA                                                                                    >  SRR19973070.2353428/1‑150 (MQ=60)
                                                                   ATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAA                                                                                    >  SRR19973070.540065/1‑150 (MQ=60)
                                                                       TGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGG                                                                                >  SRR19973070.2898272/1‑150 (MQ=60)
                                                                            CGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTC                                                                           <  SRR19973070.1942876/150‑1 (MQ=60)
                                                                            CGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTC                                                                           <  SRR19973070.1942855/150‑1 (MQ=60)
                                                                                  GGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAA                                                                     <  SRR19973070.2260798/150‑1 (MQ=60)
                                                                                    CTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAAT                                                                   <  SRR19973070.3318425/150‑1 (MQ=60)
                                                                                       CCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCT                                                                <  SRR19973070.227944/150‑1 (MQ=60)
                                                                                         GCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCG                                                              <  SRR19973070.1586120/150‑1 (MQ=60)
                                                                                          CGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGA                                                             >  SRR19973070.3322910/1‑150 (MQ=60)
                                                                                          CGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGA                                                             >  SRR19973070.1746594/1‑150 (MQ=60)
                                                                                           GTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGGACTTCTCGAG                                                            >  SRR19973070.1698998/1‑150 (MQ=60)
                                                                                           GTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAG                                                            >  SRR19973070.7749/1‑150 (MQ=60)
                                                                                               ATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCTTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAA                                                        >  SRR19973070.2383189/1‑150 (MQ=60)
                                                                                               ATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAA                                                        >  SRR19973070.2656801/1‑150 (MQ=60)
                                                                                                  ACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCG                                                     <  SRR19973070.1335247/150‑1 (MQ=60)
                                                                                                    TGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGTCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGAC                                                   <  SRR19973070.393067/150‑1 (MQ=60)
                                                                                                    TGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGAC                                                   <  SRR19973070.1030069/150‑1 (MQ=60)
                                                                                                     GCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACG                                                  <  SRR19973070.457928/150‑1 (MQ=60)
                                                                                                     GCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACG                                                  <  SRR19973070.2134770/150‑1 (MQ=60)
                                                                                                        TCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACG                                               >  SRR19973070.1623980/1‑150 (MQ=60)
                                                                                                         CTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGG                                              >  SRR19973070.1676610/1‑150 (MQ=60)
                                                                                                         CTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGG                                              >  SRR19973070.575728/1‑150 (MQ=60)
                                                                                                             AACCTGGGCGGCGGGGCGCATGAATTTGGCGTGCGTCAGGAGGCGCATTATCGGTGGAACCGCTTTGGCAGCTCCCACCGCGTGGGTTTTGTCGCTATTATTTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAG                                          <  SRR19973070.2685241/150‑1 (MQ=60)
                                                                                                             AACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGACCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAG                                          <  SRR19973070.3300579/150‑1 (MQ=60)
                                                                                                               CCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGAT                                        >  SRR19973070.2659402/1‑150 (MQ=60)
                                                                                                                CTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATG                                       <  SRR19973070.1028712/150‑1 (MQ=60)
                                                                                                                   gggggcgcggcgatttaatTTGGCGTGCGGCTGGTGGCGCATTTTATTTGGAACCGATTGGCCAGCTCCCACCGCGTGCTTTTTGTCGATTTTAATTTCGAACCGGTCGTCGGTGAATTTCTCTATAAAATCGACGACGGTCAGATGGGC                                    <  SRR19973070.2251086/131‑1 (MQ=52)
                                                                                                                   GGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGC                                    >  SRR19973070.1563689/1‑150 (MQ=60)
                                                                                                                    GCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCG                                   <  SRR19973070.2671760/150‑1 (MQ=60)
                                                                                                                     CGGCGCGGCGCATGAATTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGT                                  <  SRR19973070.52005/150‑1 (MQ=60)
                                                                                                                      GGCGCGGCGCATGAATTTGGCGTGCGTCAGGAGGCGCATTATCGGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCTTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTT                                 <  SRR19973070.2726924/150‑1 (MQ=60)
                                                                                                                        CGCGGCGCATGAAATTGGCGTGCGTCTGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTAT                               <  SRR19973070.759780/150‑1 (MQ=60)
                                                                                                                        CGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGAATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTAT                               <  SRR19973070.760708/150‑1 (MQ=60)
                                                                                                                          CGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCC                             >  SRR19973070.2789027/1‑150 (MQ=60)
                                                                                                                           GGCGCATTAAATTGGGGTGCGTCTGGAGGCGATTTATCTGTGGAACCGCTTTGGCAGGTACAACCGCGTGGGTTTTTTCGCTATTAATTTCGAACCGGTCTTCGGGGAAAGTCTGGAGAAAATCGACGACGGTCAGATGGGCGTTTTCCT                            <  SRR19973070.1661530/150‑1 (MQ=60)
                                                                                                                                ATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAAC                       >  SRR19973070.717676/1‑150 (MQ=60)
                                                                                                                                 TGAAATTGGCGTGCGTCAGGAGGCTCATTATCTGTGGAACCGATTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACG                      <  SRR19973070.1365963/150‑1 (MQ=60)
                                                                                                                                 TGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACG                      <  SRR19973070.1365999/150‑1 (MQ=60)
                                                                                                                                 TGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACG                      >  SRR19973070.3255049/1‑150 (MQ=60)
                                                                                                                                 TGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACG                      >  SRR19973070.1607406/1‑150 (MQ=60)
                                                                                                                                    AATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTAT                   <  SRR19973070.2727364/150‑1 (MQ=60)
                                                                                                                                     ttgGGCGTGCGTCCGGAGGCGCATTATCTGTGGAACCGATTTGGCAGCTCCCACCGGGTGCTTTTTGTCGATATTAATTTCGAACGGGTGGTCGGGGAAATTCTCGACAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATG                  <  SRR19973070.564008/147‑1 (MQ=60)
                                                                                                                                     ATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATG                  >  SRR19973070.408085/1‑150 (MQ=60)
                                                                                                                                     ATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATG                  >  SRR19973070.243526/1‑150 (MQ=60)
                                                                                                                                       TGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCTGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGAATGAT                >  SRR19973070.811351/1‑150 (MQ=60)
                                                                                                                                       TGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGAT                >  SRR19973070.898834/1‑150 (MQ=60)
                                                                                                                                                  AGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCG     >  SRR19973070.1809025/1‑150 (MQ=60)
                                                                                                                                                  AGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCG     >  SRR19973070.1928386/1‑150 (MQ=60)
                                                                                                                                                     AGGCGCATTATCTGTGGACCCGCTTGGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGACCGGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCAT  <  SRR19973070.38942/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCAT  >  CP009273/437523‑437821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑