Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 437,672 T→A 100% V223E (GTG→GAG)  thiI → tRNA s(4)U8 sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273437,6720TA100.0% 587.0 / ‑6.7 184V223E (GTG→GAG) thiItRNA s(4)U8 sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (74/109);  total (75/109)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

ACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTG  >  CP009273/437530‑437815
                                                                                                                                              |                                                                                                                                               
 cccAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCa                                                                                                                                           <  2:1596227/148‑1 (MQ=255)
 cccAGGAAGATGTGCTGTCGCTCAGTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCa                                                                                                                                           <  2:1595915/148‑1 (MQ=255)
  ccAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCAt                                                                                                                                          <  1:2925224/148‑1 (MQ=255)
   cAGGAAGATGTGCTGTCGCGCATTTCCGGTGGTTTCGACTCCGGTGTTTGCAGTTATATGTTTTTTCGTCGCGGCTGCCGCGTGCATTACTGCTTGTTTATACACGGCGGCGCGGCGCATGAAAATGGCGTGCGGGAGGTGgggccgt                                                                                                                                         >  1:3216654/1‑145 (MQ=255)
    aGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGGTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTa                                                                                                                                        >  1:1226397/1‑148 (MQ=255)
     ggAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTAt                                                                                                                                       >  2:3387249/1‑148 (MQ=255)
     ggAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTAAGGCTTCTTTAACCTCGGCGGCGCGGCGCATTAAATTGGCGTGCGTCAGGAGGCGCATTAt                                                                                                                                       >  1:297085/1‑148 (MQ=255)
      gAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATc                                                                                                                                      <  1:1042964/148‑1 (MQ=255)
      gAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATc                                                                                                                                      <  2:1714356/148‑1 (MQ=255)
             tgCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCTCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGaa                                                                                                                               <  1:1142845/148‑1 (MQ=255)
             tgCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGaa                                                                                                                               <  2:3085488/148‑1 (MQ=255)
                tGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCg                                                                                                                            <  2:2975910/148‑1 (MQ=255)
                 gTCGCTAATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGc                                                                                                                           <  1:3687015/148‑1 (MQ=255)
                  tCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCt                                                                                                                          <  1:3434239/148‑1 (MQ=255)
                  tCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCt                                                                                                                          <  1:1983077/148‑1 (MQ=255)
                  tCGCTCATGTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCt                                                                                                                          <  2:3199858/148‑1 (MQ=255)
                   cGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCtt                                                                                                                         <  2:3479757/148‑1 (MQ=255)
                     cTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTg                                                                                                                       >  2:1188222/1‑148 (MQ=255)
                       cATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGc                                                                                                                     >  2:132489/1‑148 (MQ=255)
                        aTTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTGTGGCa                                                                                                                    >  2:2703840/1‑148 (MQ=255)
                          ttCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGc                                                                                                                  <  2:3550376/148‑1 (MQ=255)
                          ttCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGc                                                                                                                  >  1:777709/1‑148 (MQ=255)
                             cGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTcc                                                                                                               <  1:2610864/148‑1 (MQ=255)
                               gtggtTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCa                                                                                                             >  2:2146011/1‑148 (MQ=255)
                               gtggtTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCa                                                                                                             >  1:3569664/1‑148 (MQ=255)
                                tggtTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCAc                                                                                                            >  1:3422033/1‑148 (MQ=255)
                                tggtTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCAc                                                                                                            >  1:1072180/1‑148 (MQ=255)
                                tggtTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCAc                                                                                                            >  2:2670323/1‑148 (MQ=255)
                                    ttCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACcgcg                                                                                                        >  2:2581490/1‑148 (MQ=255)
                                       gACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCgcgtgc                                                                                                     <  2:3664037/148‑1 (MQ=255)
                                          tCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGtt                                                                                                  <  2:593681/148‑1 (MQ=255)
                                          tCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGtt                                                                                                  <  2:2470359/148‑1 (MQ=255)
                                                gtTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCg                                                                                            >  2:1529297/1‑148 (MQ=255)
                                                gtTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCg                                                                                            >  2:2208075/1‑148 (MQ=255)
                                                  ttCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCt                                                                                          >  2:1931433/1‑148 (MQ=255)
                                                    ccAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTAt                                                                                        <  1:485778/148‑1 (MQ=255)
                                                      aGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTa                                                                                      >  1:303784/1‑148 (MQ=255)
                                                      aGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTa                                                                                      >  2:2883196/1‑148 (MQ=255)
                                                      aGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGAGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTa                                                                                      <  1:3536444/148‑1 (MQ=255)
                                                       gTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTaa                                                                                     <  1:3066662/148‑1 (MQ=255)
                                                        ttATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAt                                                                                    >  1:3625000/1‑148 (MQ=255)
                                                        ttATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAt                                                                                    >  1:1900004/1‑148 (MQ=255)
                                                        ttATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAt                                                                                    >  2:1108744/1‑148 (MQ=255)
                                                          atatGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAttt                                                                                  <  1:868013/148‑1 (MQ=255)
                                                             tGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGa                                                                               <  2:3325191/148‑1 (MQ=255)
                                                              gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGaa                                                                              >  2:910258/1‑148 (MQ=255)
                                                              gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGaa                                                                              <  1:1208134/148‑1 (MQ=255)
                                                              gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGaa                                                                              <  1:3112683/148‑1 (MQ=255)
                                                                tGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAAcc                                                                            >  2:47685/1‑148 (MQ=255)
                                                                  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCgg                                                                          <  1:1871521/148‑1 (MQ=255)
                                                                   tGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGgt                                                                         <  1:910258/148‑1 (MQ=255)
                                                                        cgcgGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGtcgtcg                                                                    <  1:3115614/148‑1 (MQ=255)
                                                                          cgGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCggg                                                                  <  1:3117553/148‑1 (MQ=255)
                                                                          cgGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGGGTAACAGCTTTGGCCGCGAACACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCggg                                                                  <  1:3745835/148‑1 (MQ=255)
                                                                              tGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGaaa                                                              <  1:1732636/148‑1 (MQ=255)
                                                                              tGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGaaa                                                              <  1:2809286/148‑1 (MQ=255)
                                                                              tGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGaaa                                                              <  2:1742406/148‑1 (MQ=255)
                                                                                ccGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAAtt                                                            <  2:1409527/148‑1 (MQ=255)
                                                                                ccGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAAtt                                                            <  1:456615/148‑1 (MQ=255)
                                                                                 cgcgTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATtc                                                           <  1:1961640/148‑1 (MQ=255)
                                                                                     tGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCga                                                       >  2:1839629/1‑148 (MQ=255)
                                                                                      gCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCgag                                                      >  1:251851/1‑148 (MQ=255)
                                                                                        aTTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGaa                                                    <  1:2208075/148‑1 (MQ=255)
                                                                                           aCTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAAt                                                 >  1:602440/1‑148 (MQ=255)
                                                                                           aCTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAAt                                                 <  2:23609/148‑1 (MQ=255)
                                                                                           aCTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAAt                                                 <  2:23581/148‑1 (MQ=255)
                                                                                           aCTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCCCGAGAAAAt                                                 <  2:2153404/148‑1 (MQ=255)
                                                                                             tGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcg                                               <  2:633503/148‑1 (MQ=255)
                                                                                             tGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcg                                               <  1:419331/148‑1 (MQ=255)
                                                                                                ttcttTACCCTCGGCGGCGCGGCGCATGAAATTGGCGTGCTTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACGGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgacg                                            <  2:2256222/148‑1 (MQ=255)
                                                                                                ttcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgacg                                            >  1:2121543/1‑148 (MQ=255)
                                                                                                ttcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgacg                                            <  2:1372927/148‑1 (MQ=255)
                                                                                                 tcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgacga                                           >  1:2945983/1‑148 (MQ=255)
                                                                                                 tcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgacga                                           >  1:1425974/1‑148 (MQ=255)
                                                                                                 tcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgacga                                           >  1:1724019/1‑148 (MQ=255)
                                                                                                     tAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGt                                       <  2:1260273/148‑1 (MQ=255)
                                                                                                        catCGGCGGCGCGGCGAATGAAATTGGCTTGCGTCAGGAGGCGCATTATCTGTGGAACGGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAg                                    <  2:1577576/146‑1 (MQ=255)
                                                                                                         cTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGa                                   >  2:1963897/1‑148 (MQ=255)
                                                                                                         cTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGa                                   >  2:3525314/1‑148 (MQ=255)
                                                                                                                 cgcggcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGtt                           <  2:328321/148‑1 (MQ=255)
                                                                                                                 cgcggcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGGGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGtt                           <  1:2670195/148‑1 (MQ=255)
                                                                                                                  gcggcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTa                          <  2:2173709/148‑1 (MQ=255)
                                                                                                                    ggcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATc                        <  2:777709/148‑1 (MQ=255)
                                                                                                                         aTGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaa                   <  2:1051607/148‑1 (MQ=255)
                                                                                                                         aTGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaa                   <  2:3319250/148‑1 (MQ=255)
                                                                                                                          tGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaaa                  >  1:2499437/1‑148 (MQ=255)
                                                                                                                           gAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaaaa                 >  1:267929/1‑147 (MQ=255)
                                                                                                                           gAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAAc                 >  1:268227/1‑148 (MQ=255)
                                                                                                                             aaTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGt               <  2:1226397/148‑1 (MQ=255)
                                                                                                                              tttGGCGTGCGTCTGGAGGCGCATTATCTTTGTAACCGCTTTGGCAGCTCCCCCGGGGTGGGTTTTGTCGCAATTAATTTCCAACCGGTCGTGGGGTTAATTCTCGAGAAAATCGACGACGGTGAGATGGGCGTTATCCTCAAACGTa              <  1:62257/147‑1 (MQ=255)
                                                                                                                              aTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTa              >  1:302186/1‑148 (MQ=255)
                                                                                                                              aTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTa              <  2:3693457/148‑1 (MQ=255)
                                                                                                                              aTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTa              <  2:1312787/148‑1 (MQ=255)
                                                                                                                                tGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGATATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatg            <  1:1509458/148‑1 (MQ=255)
                                                                                                                                 ggCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatga           <  2:2617756/148‑1 (MQ=255)
                                                                                                                                   cgtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatgatg         <  1:1251381/148‑1 (MQ=255)
                                                                                                                                    gtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATgg        <  1:1968972/148‑1 (MQ=255)
                                                                                                                                    gtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATgg        <  1:2146011/148‑1 (MQ=255)
                                                                                                                                        gtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgtgcg    <  2:2347931/148‑1 (MQ=255)
                                                                                                                                          cAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgtgcgtg  <  2:2148389/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
ACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTG  >  CP009273/437530‑437815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGC  >  CP009273/437524‑437819
                                                                                                                                                    |                                                                                                                                                   
ATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAG                                                                                                                                                    <  SRR19973069.2925934/150‑1 (MQ=60)
 TCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGG                                                                                                                                                   <  SRR19973069.2855731/150‑1 (MQ=60)
  CGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGC                                                                                                                                                  <  SRR19973069.3601548/150‑1 (MQ=60)
     CACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTGCAGGTATATGTTGATGCGTCGCGGCTGGCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCCTGAAATTGGCGTGGGTCAGGAGGCGCA                                                                                                                                               >  SRR19973069.3341009/1‑150 (MQ=60)
      ACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCAT                                                                                                                                              <  SRR19973069.3753673/150‑1 (MQ=60)
        CCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTA                                                                                                                                            <  SRR19973069.2944609/150‑1 (MQ=60)
        CCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAAACTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTA                                                                                                                                            >  SRR19973069.1236536/1‑150 (MQ=60)
        CCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGGTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTA                                                                                                                                            >  SRR19973069.1234772/1‑150 (MQ=60)
         CAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTAAGGCTTCTTTAACCTCGGCGGCGCGGCGCATTAAATTGGCGTGCGTCAGGAGGCGCATTAT                                                                                                                                           >  SRR19973069.299110/1‑150 (MQ=60)
         CAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTGATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTAT                                                                                                                                           >  SRR19973069.298662/1‑150 (MQ=60)
            GAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTG                                                                                                                                        <  SRR19973069.1050211/150‑1 (MQ=60)
              AGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTG                                                                                                                                      >  SRR19973069.1268771/1‑150 (MQ=60)
                   TGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCTCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACC                                                                                                                                 <  SRR19973069.1150824/150‑1 (MQ=60)
                     CTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGC                                                                                                                               >  SRR19973069.3109307/1‑150 (MQ=60)
                       GTCGCTAATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTT                                                                                                                             <  SRR19973069.3711128/150‑1 (MQ=60)
                        TCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTT                                                                                                                            <  SRR19973069.3456557/150‑1 (MQ=60)
                        TCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTT                                                                                                                            <  SRR19973069.3456518/150‑1 (MQ=60)
                        TCGCTCATGTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTT                                                                                                                            <  SRR19973069.1996870/150‑1 (MQ=60)
                            TCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCA                                                                                                                        >  SRR19973069.802987/1‑150 (MQ=60)
                             CATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAG                                                                                                                       <  SRR19973069.2883008/150‑1 (MQ=60)
                             CATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAG                                                                                                                       <  SRR19973069.258398/150‑1 (MQ=60)
                              ATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGC                                                                                                                      >  SRR19973069.637680/1‑150 (MQ=60)
                              ATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGC                                                                                                                      >  SRR19973069.783222/1‑150 (MQ=60)
                                   CGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCA                                                                                                                 >  SRR19973069.3592917/1‑150 (MQ=60)
                                   CGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCA                                                                                                                 <  SRR19973069.2628319/150‑1 (MQ=60)
                                    GGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCAC                                                                                                                >  SRR19973069.3444300/1‑150 (MQ=60)
                                    GGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCAC                                                                                                                >  SRR19973069.1079659/1‑150 (MQ=60)
                                              ACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTT                                                                                                      <  SRR19973069.1989025/150‑1 (MQ=60)
                                                        TTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTAT                                                                                            <  SRR19973069.3144939/150‑1 (MQ=60)
                                                        TTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTAT                                                                                            >  SRR19973069.3064543/1‑150 (MQ=60)
                                                        TTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTAT                                                                                            >  SRR19973069.1650111/1‑150 (MQ=60)
                                                          CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTA                                                                                          <  SRR19973069.1914837/150‑1 (MQ=60)
                                                          CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTA                                                                                          <  SRR19973069.489289/150‑1 (MQ=60)
                                                          CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTA                                                                                          >  SRR19973069.305837/1‑150 (MQ=60)
                                                          CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTA                                                                                          >  SRR19973069.305488/1‑150 (MQ=60)
                                                            AGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAT                                                                                        >  SRR19973069.2188547/1‑150 (MQ=60)
                                                            AGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAT                                                                                        >  SRR19973069.1913146/1‑150 (MQ=60)
                                                            AGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAT                                                                                        >  SRR19973069.3648694/1‑150 (MQ=60)
                                                            AGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAT                                                                                        >  SRR19973069.3648333/1‑150 (MQ=60)
                                                            AGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGAGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAT                                                                                        <  SRR19973069.3559374/150‑1 (MQ=60)
                                                             GTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATT                                                                                       <  SRR19973069.3553398/150‑1 (MQ=60)
                                                             GTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATT                                                                                       <  SRR19973069.3086933/150‑1 (MQ=60)
                                                               TATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTC                                                                                     <  SRR19973069.448386/150‑1 (MQ=60)
                                                                ATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCG                                                                                    <  SRR19973069.874061/150‑1 (MQ=60)
                                                                    GTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACC                                                                                <  SRR19973069.1216463/150‑1 (MQ=60)
                                                                    GTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACC                                                                                <  SRR19973069.1216430/150‑1 (MQ=60)
                                                                    GTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACC                                                                                <  SRR19973069.3133152/150‑1 (MQ=60)
                                                                        ATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTC                                                                            <  SRR19973069.1884549/150‑1 (MQ=60)
                                                                        ATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTC                                                                            <  SRR19973069.1884557/150‑1 (MQ=60)
                                                                         TGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCG                                                                           <  SRR19973069.916494/150‑1 (MQ=60)
                                                                         TGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCG                                                                           <  SRR19973069.2816023/150‑1 (MQ=60)
                                                                              CGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGG                                                                      <  SRR19973069.3136089/150‑1 (MQ=60)
                                                                              CGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGG                                                                      <  SRR19973069.3136117/150‑1 (MQ=60)
                                                                                CGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGA                                                                    <  SRR19973069.3138039/150‑1 (MQ=60)
                                                                                CGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGGGTAACAGCTTTGGCCGCGAACACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGA                                                                    <  SRR19973069.3770235/150‑1 (MQ=60)
                                                                                    TGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATT                                                                <  SRR19973069.2827905/150‑1 (MQ=60)
                                                                                    TGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATT                                                                <  SRR19973069.1744777/150‑1 (MQ=60)
                                                                                    TGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATT                                                                <  SRR19973069.1744786/150‑1 (MQ=60)
                                                                                    TGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATT                                                                <  SRR19973069.900766/150‑1 (MQ=60)
                                                                                      CCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCT                                                              <  SRR19973069.459960/150‑1 (MQ=60)
                                                                                       CGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTC                                                             <  SRR19973069.1975236/150‑1 (MQ=60)
                                                                                          GTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGACATTCTCGAG                                                          >  SRR19973069.2052217/1‑150 (MQ=60)
                                                                                          GTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAG                                                          <  SRR19973069.566472/150‑1 (MQ=60)
                                                                                          GTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAG                                                          >  SRR19973069.934964/1‑150 (MQ=60)
                                                                                          GTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAG                                                          >  SRR19973069.253696/1‑150 (MQ=60)
                                                                                            GCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAA                                                        >  SRR19973069.383816/1‑150 (MQ=60)
                                                                                             CATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAA                                                       >  SRR19973069.1122790/1‑150 (MQ=60)
                                                                                              ATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAA                                                      <  SRR19973069.2223048/150‑1 (MQ=60)
                                                                                               TTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAAT                                                     >  SRR19973069.606495/1‑150 (MQ=60)
                                                                                                 ACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCG                                                   >  SRR19973069.1180081/1‑150 (MQ=60)
                                                                                                   TGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGAC                                                 <  SRR19973069.422407/150‑1 (MQ=60)
                                                                                                    GCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGACATTCTCGAGAAAATCGACG                                                >  SRR19973069.2136153/1‑150 (MQ=60)
                                                                                                     CTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGA                                               >  SRR19973069.1435940/1‑150 (MQ=60)
                                                                                                     CTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGA                                               >  SRR19973069.1736099/1‑150 (MQ=60)
                                                                                                     CTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGA                                               >  SRR19973069.2965532/1‑150 (MQ=60)
                                                                                                       TCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACG                                             >  SRR19973069.626355/1‑150 (MQ=60)
                                                                                                           TAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCA                                         >  SRR19973069.1795382/1‑150 (MQ=60)
                                                                                                             ACCTCGGCGGCGCGGCGCATGAATTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGA                                       <  SRR19973069.3668440/150‑1 (MQ=60)
                                                                                                                       CGCGGCGCATGAATTTGGCGTGCGTCAGGAGGCGCTTTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCTTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTAT                             <  SRR19973069.2688229/150‑1 (MQ=60)
                                                                                                                       CGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGGGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTAT                             <  SRR19973069.2688099/150‑1 (MQ=60)
                                                                                                                              CATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAA                      >  SRR19973069.2514485/1‑150 (MQ=60)
                                                                                                                              CATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAA                      >  SRR19973069.2516133/1‑150 (MQ=60)
                                                                                                                               ATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAAC                     >  SRR19973069.270134/1‑150 (MQ=60)
                                                                                                                               ATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAAC                     >  SRR19973069.2152527/1‑150 (MQ=60)
                                                                                                                               ATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAAC                     >  SRR19973069.269839/1‑150 (MQ=60)
                                                                                                                               ATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAAA                     >  SRR19973069.269836/1‑150 (MQ=60)
                                                                                                                                TGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACG                    >  SRR19973069.264540/1‑150 (MQ=60)
                                                                                                                                  AAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTA                  >  SRR19973069.303554/1‑150 (MQ=60)
                                                                                                                                  AAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTA                  >  SRR19973069.304231/1‑150 (MQ=60)
                                                                                                                                    TTTGGCGTGCGTCTGGAGGCGCATTATCTTTGTAACCGCTTTGGCAGCTCCCCCGGGGTGGGTTTTGTCGCAATTAATTTCCAACCGGTCGTGGGGTTAATTCTCGAGAAAATCGACGACGGTGAGATGGGCGTTATCCTCAAACGTATG                <  SRR19973069.62991/150‑1 (MQ=60)
                                                                                                                                      TGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGATATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGAT              <  SRR19973069.1519897/150‑1 (MQ=60)
                                                                                                                                         CGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGT           <  SRR19973069.1259853/150‑1 (MQ=60)
                                                                                                                                          GTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTG          <  SRR19973069.1982640/150‑1 (MQ=60)
                                                                                                                                          GTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTG          <  SRR19973069.2160730/150‑1 (MQ=60)
                                                                                                                                                 AGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAAACGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTAAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGACG   >  SRR19973069.3046055/1‑150 (MQ=60)
                                                                                                                                                  GGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGC  >  SRR19973069.3695808/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
ATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGC  >  CP009273/437524‑437819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑