Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,122,013 G→T 94.6% T119T (ACC→ACA)  wcaF ← putative acyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,122,0130GT94.6% 540.5 / 20.2 184T119T (ACC→ACA) wcaFputative acyl transferase
Reads supporting (aligned to +/- strand):  ref base G (7/3);  new base T (72/102);  total (79/105)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 185 total aligned reads displayed.

GGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAT  >  CP009273/2121868‑2122157
                                                                                                                                                 |                                                                                                                                                
ggCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGt                                                                                                                                                <  1:3120320/148‑1 (MQ=255)
 gCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATtgtg                                                                                                                                               <  1:8868/148‑1 (MQ=255)
  cAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGa                                                                                                                                              >  1:2712306/1‑148 (MQ=255)
   aaaCCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGaa                                                                                                                                             <  1:2453809/148‑1 (MQ=255)
      ccACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATg                                                                                                                                          >  2:891108/1‑148 (MQ=255)
         caTTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTg                                                                                                                                       <  2:5513/148‑1 (MQ=255)
         caTTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTg                                                                                                                                       <  1:3071580/148‑1 (MQ=255)
          aTTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGa                                                                                                                                      >  2:2883914/1‑148 (MQ=255)
           tttGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGAc                                                                                                                                     <  1:1321265/148‑1 (MQ=255)
            ttGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACACTAGGCGTGGCGTTAATTGTGAAATGTTGACt                                                                                                                                    >  1:2131847/1‑148 (MQ=255)
            ttGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACt                                                                                                                                    <  2:114663/148‑1 (MQ=255)
            ttGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACt                                                                                                                                    <  2:1631415/148‑1 (MQ=255)
                cGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCa                                                                                                                                <  1:687489/148‑1 (MQ=255)
                 ggAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCAt                                                                                                                               <  2:1454577/148‑1 (MQ=255)
                 ggAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCAt                                                                                                                               <  2:1454605/148‑1 (MQ=255)
                  gAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATg                                                                                                                              >  2:190729/1‑148 (MQ=255)
                  gAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATg                                                                                                                              <  1:212455/148‑1 (MQ=255)
                   aaGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATgg                                                                                                                             <  2:1435708/148‑1 (MQ=255)
                   aaGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATgg                                                                                                                             >  2:629889/1‑148 (MQ=255)
                   aaGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGGTAATTGTGAAATGTTGACTTGCATgg                                                                                                                             >  1:772697/1‑148 (MQ=255)
                      cGATTTAAAAACACTGCTTCGTGCACCCACGTCGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCTGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCg                                                                                                                          <  1:2419766/148‑1 (MQ=255)
                        aTTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTg                                                                                                                        <  2:430824/148‑1 (MQ=255)
                         tttAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTgg                                                                                                                       >  2:321314/1‑148 (MQ=255)
                          ttAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGc                                                                                                                      <  1:2820126/148‑1 (MQ=255)
                          ttAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGc                                                                                                                      >  2:2302070/1‑148 (MQ=255)
                          ttAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGc                                                                                                                      <  1:456189/148‑1 (MQ=255)
                             aaaaCACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTAc                                                                                                                   >  2:1560373/1‑148 (MQ=255)
                              aaaCACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTAcc                                                                                                                  >  1:1013845/1‑148 (MQ=255)
                              aaaCACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTAcc                                                                                                                  >  2:1782056/1‑148 (MQ=255)
                              aaaCACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTAcc                                                                                                                  >  2:214991/1‑148 (MQ=255)
                              aaaCACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTAcc                                                                                                                  >  2:422089/1‑148 (MQ=255)
                                  acTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTg                                                                                                              <  2:1630301/148‑1 (MQ=255)
                                  acTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTg                                                                                                              >  2:933623/1‑148 (MQ=255)
                                      cTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCata                                                                                                          <  2:265853/148‑1 (MQ=255)
                                        tCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaa                                                                                                        <  2:1934971/148‑1 (MQ=255)
                                        tCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaa                                                                                                        >  2:12472/1‑148 (MQ=255)
                                           tGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaataa                                                                                                     <  2:1014229/148‑1 (MQ=255)
                                           tGCACCCACGACGGTGCCGTCGCCGAGTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaataa                                                                                                     <  2:2598084/148‑1 (MQ=255)
                                            gCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAAc                                                                                                    <  1:422089/148‑1 (MQ=255)
                                            gCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAAc                                                                                                    >  1:2990088/1‑148 (MQ=255)
                                             cACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACt                                                                                                   <  2:2290382/148‑1 (MQ=255)
                                             cACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACt                                                                                                   >  2:441178/1‑148 (MQ=255)
                                              aCCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACtt                                                                                                  <  1:1544346/148‑1 (MQ=255)
                                                ccACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACtttt                                                                                                >  2:1715190/1‑148 (MQ=255)
                                                 cACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACttttt                                                                                               <  2:3094371/148‑1 (MQ=255)
                                                     acgGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCg                                                                                           <  1:2959014/148‑1 (MQ=255)
                                                         tGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCTGTTGCCAGCCAGCATGTCTCGCCAATCACAATAGGCGTGGCGTTACTTGTGAAATGTTGACTGGCATGGTCGTGGCTACCGGTGCATACATAACTTTTTTGCGatat                                                                                       <  2:2784796/148‑1 (MQ=255)
                                                         tGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGatat                                                                                       <  2:2788873/148‑1 (MQ=255)
                                                             gTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCAcc                                                                                   <  1:2803568/148‑1 (MQ=255)
                                                                  cGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAtg                                                                              <  1:1715190/148‑1 (MQ=255)
                                                                  cGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAtg                                                                              >  2:1915991/1‑148 (MQ=255)
                                                                    aTTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAtgtg                                                                            <  1:1992461/148‑1 (MQ=255)
                                                                     ttGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTgc                                                                           >  1:847569/1‑148 (MQ=255)
                                                                      tgtgACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTgcg                                                                          >  1:1097748/1‑148 (MQ=255)
                                                                      tgtgACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTgcg                                                                          >  1:2112357/1‑148 (MQ=255)
                                                                        tgACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGcc                                                                        >  2:2402962/1‑148 (MQ=255)
                                                                         gACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCa                                                                       <  1:3257795/148‑1 (MQ=255)
                                                                         gACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCa                                                                       <  1:743326/148‑1 (MQ=255)
                                                                            gCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATg                                                                    <  2:2228418/148‑1 (MQ=255)
                                                                            gCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATg                                                                    <  2:2431306/148‑1 (MQ=255)
                                                                             ccTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATgg                                                                   >  2:789312/1‑148 (MQ=255)
                                                                                 gggCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAt                                                               <  2:717359/148‑1 (MQ=255)
                                                                                  ggCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAtt                                                              <  2:1832771/148‑1 (MQ=255)
                                                                                  ggCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAtt                                                              <  2:493584/148‑1 (MQ=255)
                                                                                    caacaaAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTc                                                            >  1:2210050/1‑148 (MQ=255)
                                                                                    caacaaAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTc                                                            >  1:547509/1‑148 (MQ=255)
                                                                                     aacaaAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCa                                                           <  1:2482910/148‑1 (MQ=255)
                                                                                          aGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCCATGGTTATTTCACCGAg                                                      >  1:2926471/1‑148 (MQ=255)
                                                                                          aGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAg                                                      <  2:864377/148‑1 (MQ=255)
                                                                                          aGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAg                                                      <  2:864219/148‑1 (MQ=255)
                                                                                            aCATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATACATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAggg                                                    >  2:3016622/1‑148 (MQ=255)
                                                                                            aCATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAggg                                                    >  2:3017267/1‑148 (MQ=255)
                                                                                               tCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGtat                                                 >  1:604355/1‑148 (MQ=255)
                                                                                               tCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGtat                                                 >  1:1922768/1‑148 (MQ=255)
                                                                                                cGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGtata                                                >  1:2576729/1‑148 (MQ=255)
                                                                                                cGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGtata                                                <  1:1842630/148‑1 (MQ=255)
                                                                                                    tGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATaaa                                            <  2:3262856/148‑1 (MQ=255)
                                                                                                          ccagcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACg                                      >  2:2691010/1‑148 (MQ=255)
                                                                                                           cagcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGt                                     >  2:1671387/1‑148 (MQ=255)
                                                                                                            agcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTc                                    <  1:198011/148‑1 (MQ=255)
                                                                                                              cATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAt                                  <  1:1156166/148‑1 (MQ=255)
                                                                                                              cATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAt                                  >  1:154037/1‑148 (MQ=255)
                                                                                                              cATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAt                                  <  1:1632416/148‑1 (MQ=255)
                                                                                                                 ttCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTCTATACATTGACGTCATCGc                               >  2:428408/1‑148 (MQ=255)
                                                                                                                       ccAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGAccc                         <  2:2112357/148‑1 (MQ=255)
                                                                                                                         aaTCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCAc                       >  1:1035042/1‑148 (MQ=255)
                                                                                                                         aaTCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCAc                       >  1:2687136/1‑148 (MQ=255)
                                                                                                                            cacaATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCAcgcg                    <  2:2955651/148‑1 (MQ=255)
                                                                                                                              caATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTa                  <  2:772697/148‑1 (MQ=255)
                                                                                                                              caATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTa                  >  1:201717/1‑148 (MQ=255)
                                                                                                                                 tAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATCGTTATTTCACCGAGGGTCTATAAATTGACGTCATCGCCGACCCACGCGTAATc               >  2:3198039/1‑148 (MQ=255)
                                                                                                                                  aGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTAACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCa              >  1:965428/1‑148 (MQ=255)
                                                                                                                                   ggcgtggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAc             <  1:914359/148‑1 (MQ=255)
                                                                                                                                      gtggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTa          <  2:2785538/148‑1 (MQ=255)
                                                                                                                                        ggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAg        >  1:369300/1‑148 (MQ=255)
                                                                                                                                        ggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAg        >  1:2394208/1‑148 (MQ=255)
                                                                                                                                          cgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGt      <  2:1324436/148‑1 (MQ=255)
                                                                                                                                          cgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGt      <  1:2034505/148‑1 (MQ=255)
                                                                                                                                              aaTTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAt  <  1:2393423/148‑1 (MQ=255)
                                                                                                                                              aaTTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAt  <  1:2063357/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAT  >  CP009273/2121868‑2122157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTC  >  CP009273/2121865‑2122160
                                                                                                                                                    |                                                                                                                                                   
CCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTG                                                                                                                                                    <  SRR19973070.3334021/150‑1 (MQ=60)
   GGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGA                                                                                                                                                 >  SRR19973070.2729836/1‑150 (MQ=60)
   GGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGA                                                                                                                                                 <  SRR19973070.3140454/150‑1 (MQ=60)
   GGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGA                                                                                                                                                 <  SRR19973070.3141036/150‑1 (MQ=60)
    GCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAA                                                                                                                                                <  SRR19973070.9001/150‑1 (MQ=60)
    GCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAA                                                                                                                                                <  SRR19973070.1132906/150‑1 (MQ=60)
      AAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAAT                                                                                                                                              <  SRR19973070.2469543/150‑1 (MQ=60)
      AAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAAT                                                                                                                                              <  SRR19973070.2469855/150‑1 (MQ=60)
            CATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGAC                                                                                                                                        <  SRR19973070.3091291/150‑1 (MQ=60)
             ATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACACTAGGCGTGGCGTTAATTGTGAAATGTTGACT                                                                                                                                       >  SRR19973070.2146095/1‑150 (MQ=60)
             ATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACT                                                                                                                                       >  SRR19973070.467088/1‑150 (MQ=60)
             ATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACT                                                                                                                                       >  SRR19973070.1705626/1‑150 (MQ=60)
              TTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTT                                                                                                                                      <  SRR19973070.1330403/150‑1 (MQ=60)
               TTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTG                                                                                                                                     <  SRR19973070.2049178/150‑1 (MQ=60)
                GGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGC                                                                                                                                    <  SRR19973070.591838/150‑1 (MQ=60)
                   CGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATG                                                                                                                                 <  SRR19973070.2670242/150‑1 (MQ=60)
                   CGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATG                                                                                                                                 <  SRR19973070.692394/150‑1 (MQ=60)
                   CGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATG                                                                                                                                 <  SRR19973070.3036237/150‑1 (MQ=60)
                   CGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATG                                                                                                                                 <  SRR19973070.1006275/150‑1 (MQ=60)
                    GGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGG                                                                                                                                >  SRR19973070.2000748/1‑150 (MQ=60)
                    GGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGG                                                                                                                                >  SRR19973070.780370/1‑150 (MQ=60)
                    GGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGG                                                                                                                                >  SRR19973070.2142323/1‑150 (MQ=60)
                    GGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGGTAATTGTGAAATGTTGACTTGCATGG                                                                                                                                >  SRR19973070.778114/1‑150 (MQ=60)
                     GAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGT                                                                                                                               <  SRR19973070.214020/150‑1 (MQ=60)
                     GAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGT                                                                                                                               <  SRR19973070.340901/150‑1 (MQ=60)
                         CGATTTAAAAACACTGCTTCGTGCACCCACGTCGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCTGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTG                                                                                                                           <  SRR19973070.2435682/150‑1 (MQ=60)
                             TTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTA                                                                                                                       <  SRR19973070.2838355/150‑1 (MQ=60)
                             TTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTA                                                                                                                       <  SRR19973070.459460/150‑1 (MQ=60)
                               AAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACC                                                                                                                     >  SRR19973070.1021049/1‑150 (MQ=60)
                                AAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCG                                                                                                                    <  SRR19973070.2126983/150‑1 (MQ=60)
                                 AAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGG                                                                                                                   <  SRR19973070.21376/150‑1 (MQ=60)
                                     ACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCA                                                                                                               <  SRR19973070.276788/150‑1 (MQ=60)
                                          TTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAAT                                                                                                          <  SRR19973070.2989722/150‑1 (MQ=60)
                                             GTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAAC                                                                                                       >  SRR19973070.3009243/1‑150 (MQ=60)
                                               GCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTT                                                                                                     <  SRR19973070.425168/150‑1 (MQ=60)
                                                 ACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTT                                                                                                   <  SRR19973070.1555197/150‑1 (MQ=60)
                                                        ACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGAT                                                                                            <  SRR19973070.2978048/150‑1 (MQ=60)
                                                        ACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGAT                                                                                            <  SRR19973070.2481296/150‑1 (MQ=60)
                                                          GGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATAT                                                                                          <  SRR19973070.2507739/150‑1 (MQ=60)
                                                                GTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGA                                                                                    <  SRR19973070.2821682/150‑1 (MQ=60)
                                                                     CGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTG                                                                               <  SRR19973070.1727046/150‑1 (MQ=60)
                                                                     CGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTG                                                                               <  SRR19973070.2151104/150‑1 (MQ=60)
                                                                     CGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTG                                                                               >  SRR19973070.1913780/1‑150 (MQ=60)
                                                                      GATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGC                                                                              >  SRR19973070.1810252/1‑150 (MQ=60)
                                                                      GATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGC                                                                              >  SRR19973070.853362/1‑150 (MQ=60)
                                                                       ATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCG                                                                             <  SRR19973070.2005984/150‑1 (MQ=60)
                                                                       ATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCG                                                                             >  SRR19973070.1105264/1‑150 (MQ=60)
                                                                       ATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCG                                                                             <  SRR19973070.2005949/150‑1 (MQ=60)
                                                                       ATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCG                                                                             >  SRR19973070.1105306/1‑150 (MQ=60)
                                                                       ATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCG                                                                             >  SRR19973070.2126519/1‑150 (MQ=60)
                                                                            GACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAAT                                                                        <  SRR19973070.748581/150‑1 (MQ=60)
                                                                            GACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAAT                                                                        <  SRR19973070.748644/150‑1 (MQ=60)
                                                                            GACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAAT                                                                        <  SRR19973070.3278774/150‑1 (MQ=60)
                                                                             ACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATG                                                                       <  SRR19973070.2270728/150‑1 (MQ=60)
                                                                                     GGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTC                                                               >  SRR19973070.2224590/1‑150 (MQ=60)
                                                                                     GGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTC                                                               >  SRR19973070.551161/1‑150 (MQ=60)
                                                                                        AACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACC                                                            <  SRR19973070.2499058/150‑1 (MQ=60)
                                                                                           AAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAG                                                         >  SRR19973070.2945428/1‑150 (MQ=60)
                                                                                           AAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATAACCGAATGTGCGCCCATGGTTATTTCACCGAG                                                         >  SRR19973070.2945361/1‑150 (MQ=60)
                                                                                                CATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTAT                                                    >  SRR19973070.1259641/1‑150 (MQ=60)
                                                                                                CATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTAT                                                    <  SRR19973070.1554322/150‑1 (MQ=60)
                                                                                                CATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTAT                                                    >  SRR19973070.608575/1‑150 (MQ=60)
                                                                                                CATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTAT                                                    >  SRR19973070.1935734/1‑150 (MQ=60)
                                                                                                 ATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATA                                                   >  SRR19973070.2593574/1‑150 (MQ=60)
                                                                                                   CGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATA                                                 <  SRR19973070.1855275/150‑1 (MQ=60)
                                                                                                   CGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATA                                                 <  SRR19973070.2419968/150‑1 (MQ=60)
                                                                                                    GGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAA                                                >  SRR19973070.736106/1‑150 (MQ=60)
                                                                                                        GCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTG                                            <  SRR19973070.897400/150‑1 (MQ=60)
                                                                                                             CCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTC                                       >  SRR19973070.430879/1‑150 (MQ=60)
                                                                                                               AGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAT                                     <  SRR19973070.199520/150‑1 (MQ=60)
                                                                                                               AGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAT                                     >  SRR19973070.155324/1‑150 (MQ=60)
                                                                                                               AGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGAAGTCAT                                     >  SRR19973070.19676/1‑150 (MQ=60)
                                                                                                                 CATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCG                                   <  SRR19973070.1643736/150‑1 (MQ=60)
                                                                                                                 CATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCG                                   <  SRR19973070.1164236/150‑1 (MQ=60)
                                                                                                                          CCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCAC                          >  SRR19973070.2704554/1‑150 (MQ=60)
                                                                                                                          CCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCAC                          >  SRR19973070.1042355/1‑150 (MQ=60)
                                                                                                                             ATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCG                       <  SRR19973070.417949/150‑1 (MQ=60)
                                                                                                                               CACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTA                     >  SRR19973070.203245/1‑150 (MQ=60)
                                                                                                                                 CAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAAT                   <  SRR19973070.884025/150‑1 (MQ=60)
                                                                                                                                  AATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATC                  >  SRR19973070.282985/1‑150 (MQ=60)
                                                                                                                                   ATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTAACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCA                 >  SRR19973070.972321/1‑150 (MQ=60)
                                                                                                                                      GGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCT              <  SRR19973070.920862/150‑1 (MQ=60)
                                                                                                                                         GTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAG           >  SRR19973070.372013/1‑150 (MQ=60)
                                                                                                                                         GTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAG           >  SRR19973070.2410010/1‑150 (MQ=60)
                                                                                                                                             CGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTA       <  SRR19973070.2048267/150‑1 (MQ=60)
                                                                                                                                                 AATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTT   <  SRR19973070.145537/150‑1 (MQ=60)
                                                                                                                                                 AATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTT   <  SRR19973070.2940600/150‑1 (MQ=60)
                                                                                                                                                 AATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTT   <  SRR19973070.2077281/150‑1 (MQ=60)
                                                                                                                                                 AATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTT   <  SRR19973070.2409224/150‑1 (MQ=60)
                                                                                                                                                  ATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTC  >  SRR19973070.146757/1‑150 (MQ=60)
                                                                                                                                                  ATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTC  >  SRR19973070.2991106/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATTTC  >  CP009273/2121865‑2122160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑