Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,122,013 G→T 100% T119T (ACC→ACA)  wcaF ← putative acyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,122,0130GT99.2% 849.3 / ‑2.2 256T119T (ACC→ACA) wcaFputative acyl transferase
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (104/150);  total (106/150)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 256 total aligned reads displayed.

GGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAT  >  CP009273/2121868‑2122157
                                                                                                                                                 |                                                                                                                                                
ggCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGt                                                                                                                                                >  2:106449/1‑148 (MQ=255)
     aCCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAAt                                                                                                                                           <  1:1830950/148‑1 (MQ=255)
      ccACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATg                                                                                                                                          <  2:1509020/148‑1 (MQ=255)
        acaTTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGtt                                                                                                                                        <  1:1224440/148‑1 (MQ=255)
         caTTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTg                                                                                                                                       <  2:708834/148‑1 (MQ=255)
          aTTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGa                                                                                                                                      <  1:2084965/148‑1 (MQ=255)
              gCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTg                                                                                                                                  <  2:1302940/148‑1 (MQ=255)
                cGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCa                                                                                                                                <  2:891323/148‑1 (MQ=255)
                 ggAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCAt                                                                                                                               <  1:1145290/148‑1 (MQ=255)
                   aaGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATgg                                                                                                                             >  1:810063/1‑148 (MQ=255)
                   aaGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATgg                                                                                                                             >  1:1161921/1‑148 (MQ=255)
                   aaGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATgg                                                                                                                             >  2:396926/1‑148 (MQ=255)
                   aaGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATgg                                                                                                                             <  1:1889776/148‑1 (MQ=255)
                   aaGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATgg                                                                                                                             <  1:2689265/148‑1 (MQ=255)
                    aGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGt                                                                                                                            >  2:2309023/1‑148 (MQ=255)
                     gCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTc                                                                                                                           >  2:162861/1‑148 (MQ=255)
                      cGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCg                                                                                                                          >  2:2263704/1‑148 (MQ=255)
                       gATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGt                                                                                                                         <  1:572324/148‑1 (MQ=255)
                        aTTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTg                                                                                                                        <  2:2749027/148‑1 (MQ=255)
                         tttAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTgg                                                                                                                       <  1:1541291/148‑1 (MQ=255)
                            aaaaaCACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTa                                                                                                                    <  2:737067/148‑1 (MQ=255)
                             aaaaCACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTAc                                                                                                                   >  2:2584685/1‑148 (MQ=255)
                             aaaaCACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTAc                                                                                                                   >  2:2584221/1‑148 (MQ=255)
                                acacTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCgg                                                                                                                <  2:14608/148‑1 (MQ=255)
                                 cacTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGt                                                                                                               <  1:1480578/148‑1 (MQ=255)
                                 cacTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGt                                                                                                               >  2:3514711/1‑148 (MQ=255)
                                  acTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTg                                                                                                              >  2:682820/1‑148 (MQ=255)
                                   cTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGc                                                                                                             >  1:1654282/1‑148 (MQ=255)
                                    tGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCa                                                                                                            <  1:979932/148‑1 (MQ=255)
                                     gCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCat                                                                                                           >  2:1752649/1‑148 (MQ=255)
                                     gCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCat                                                                                                           >  2:2456886/1‑148 (MQ=255)
                                        tCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaa                                                                                                        >  1:3427391/1‑148 (MQ=255)
                                         cGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaat                                                                                                       >  2:654955/1‑148 (MQ=255)
                                         cGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaat                                                                                                       <  1:939132/148‑1 (MQ=255)
                                          gTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCataaata                                                                                                      <  2:2695351/148‑1 (MQ=255)
                                               cccACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACttt                                                                                                 >  2:2907306/1‑148 (MQ=255)
                                                ccACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACtttt                                                                                                >  2:2233874/1‑148 (MQ=255)
                                                ccACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACtttt                                                                                                >  2:2992063/1‑148 (MQ=255)
                                                 cACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACttttt                                                                                               <  1:471072/148‑1 (MQ=255)
                                                 cACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACttttt                                                                                               <  2:2897605/148‑1 (MQ=255)
                                                   cgacgGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTg                                                                                             >  1:2555344/1‑148 (MQ=255)
                                                      cgGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGa                                                                                          >  2:2078608/1‑148 (MQ=255)
                                                      cgGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGa                                                                                          <  2:601388/148‑1 (MQ=255)
                                                      cgGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGa                                                                                          <  2:2392881/148‑1 (MQ=255)
                                                       ggTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGat                                                                                         <  1:1065995/148‑1 (MQ=255)
                                                       ggTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGat                                                                                         <  2:829227/148‑1 (MQ=255)
                                                       ggTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGat                                                                                         <  1:864995/148‑1 (MQ=255)
                                                         tGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGatat                                                                                       <  2:803721/148‑1 (MQ=255)
                                                          gCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATc                                                                                      <  1:3296382/148‑1 (MQ=255)
                                                              tCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCg                                                                                  >  2:52020/1‑148 (MQ=255)
                                                               cGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGa                                                                                 <  1:184244/148‑1 (MQ=255)
                                                               cGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGa                                                                                 <  1:933671/148‑1 (MQ=255)
                                                                 ccGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAt                                                                               <  2:557874/148‑1 (MQ=255)
                                                                 ccGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAt                                                                               <  1:2699625/148‑1 (MQ=255)
                                                                  cGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAtg                                                                              <  1:3374382/148‑1 (MQ=255)
                                                                   gATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAtgt                                                                             <  1:1346821/148‑1 (MQ=255)
                                                                        tgACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGAGATATCACCGAATGTGCGcc                                                                        >  2:3189687/1‑148 (MQ=255)
                                                                           cGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAAt                                                                     <  2:1365176/148‑1 (MQ=255)
                                                                             ccTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATgg                                                                   <  1:2584522/148‑1 (MQ=255)
                                                                             ccTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATgg                                                                   >  2:3315772/1‑148 (MQ=255)
                                                                             ccTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATgg                                                                   <  1:37801/148‑1 (MQ=255)
                                                                               tGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGtt                                                                 >  2:2717186/1‑148 (MQ=255)
                                                                                ggggCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTa                                                                <  1:2609655/148‑1 (MQ=255)
                                                                                 gggCATCAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAt                                                               <  2:495346/148‑1 (MQ=255)
                                                                                 gggCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAt                                                               >  1:3510600/1‑148 (MQ=255)
                                                                                  ggCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAtt                                                              >  2:3079252/1‑148 (MQ=255)
                                                                                  ggCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAtt                                                              <  1:3448969/148‑1 (MQ=255)
                                                                                    caacaaAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTc                                                            <  2:2575026/148‑1 (MQ=255)
                                                                                         aaGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGa                                                       <  1:2238741/148‑1 (MQ=255)
                                                                                             cATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGt                                                   <  2:1853889/148‑1 (MQ=255)
                                                                                               tCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGtat                                                 <  2:398414/148‑1 (MQ=255)
                                                                                                cGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGtata                                                <  2:477763/148‑1 (MQ=255)
                                                                                                cGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGtata                                                >  1:197745/1‑148 (MQ=255)
                                                                                                cGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGtata                                                <  2:3305666/148‑1 (MQ=255)
                                                                                                   ttGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATaa                                             <  1:1054432/148‑1 (MQ=255)
                                                                                                     gccagccaGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAAt                                           >  1:3187114/1‑148 (MQ=255)
                                                                                                       cagccagcATGTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTg                                         <  1:2993506/148‑1 (MQ=255)
                                                                                                        agccagcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGa                                        <  2:2753491/148‑1 (MQ=255)
                                                                                                        agccagcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGa                                        <  2:1215940/148‑1 (MQ=255)
                                                                                                           cagcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGt                                     >  1:3257994/1‑148 (MQ=255)
                                                                                                           cagcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGt                                     >  1:1867313/1‑148 (MQ=255)
                                                                                                             gcATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCa                                   <  2:2050155/148‑1 (MQ=255)
                                                                                                              cATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAt                                  >  1:2778161/1‑148 (MQ=255)
                                                                                                                tttCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCg                                >  1:3002604/1‑148 (MQ=255)
                                                                                                                tttCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCg                                >  1:2124964/1‑148 (MQ=255)
                                                                                                                 ttCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGc                               >  2:2186506/1‑148 (MQ=255)
                                                                                                                            cacaATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCAcgcg                    >  2:3014108/1‑148 (MQ=255)
                                                                                                                            cacaATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCAcgcg                    >  2:1266235/1‑148 (MQ=255)
                                                                                                                                 tAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATc               <  2:2635839/148‑1 (MQ=255)
                                                                                                                                 tAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATc               <  1:1527510/148‑1 (MQ=255)
                                                                                                                                   ggcgtggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAc             >  1:2342607/1‑148 (MQ=255)
                                                                                                                                   ggcgtggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAc             >  1:1362995/1‑148 (MQ=255)
                                                                                                                                      gtggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTa          <  1:1752649/148‑1 (MQ=255)
                                                                                                                                       tggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTaa         <  1:52/148‑1 (MQ=255)
                                                                                                                                       tggcgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTaa         >  1:1251715/1‑148 (MQ=255)
                                                                                                                                          cgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGt      <  2:45287/148‑1 (MQ=255)
                                                                                                                                          cgtTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACATAAGGt      >  2:3431612/1‑148 (MQ=255)
                                                                                                                                            ttAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTa    <  1:738926/148‑1 (MQ=255)
                                                                                                                                            ttAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTa    <  1:2845042/148‑1 (MQ=255)
                                                                                                                                             tAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTaa   >  1:669470/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAT  >  CP009273/2121868‑2122157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATT  >  CP009273/2121865‑2122158
                                                                                                                                                    |                                                                                                                                                 
CCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGGTAATTG                                                                                                                                                  >  SRR19973065.1861357/1‑150 (MQ=60)
   GGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGA                                                                                                                                               >  SRR19973065.2446346/1‑150 (MQ=60)
        ACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGT                                                                                                                                          <  SRR19973065.1843012/150‑1 (MQ=60)
           ACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGA                                                                                                                                       <  SRR19973065.1232423/150‑1 (MQ=60)
           ACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGA                                                                                                                                       <  SRR19973065.1232256/150‑1 (MQ=60)
             ATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACT                                                                                                                                     <  SRR19973065.2098328/150‑1 (MQ=60)
             ATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACT                                                                                                                                     <  SRR19973065.2098333/150‑1 (MQ=60)
               TTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTG                                                                                                                                   <  SRR19973065.2628961/150‑1 (MQ=60)
                  CCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCAT                                                                                                                                <  SRR19973065.2946297/150‑1 (MQ=60)
                  CCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCAT                                                                                                                                <  SRR19973065.2946397/150‑1 (MQ=60)
                    GGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGG                                                                                                                              >  SRR19973065.1169357/1‑150 (MQ=60)
                    GGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGG                                                                                                                              >  SRR19973065.1067839/1‑150 (MQ=60)
                    GGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGG                                                                                                                              >  SRR19973065.815409/1‑150 (MQ=60)
                    GGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGG                                                                                                                              <  SRR19973065.1152679/150‑1 (MQ=60)
                    GGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGG                                                                                                                              <  SRR19973065.1153058/150‑1 (MQ=60)
                      AAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTC                                                                                                                            <  SRR19973065.2705960/150‑1 (MQ=60)
                      AAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTC                                                                                                                            <  SRR19973065.1902202/150‑1 (MQ=60)
                      AAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTC                                                                                                                            <  SRR19973065.1902371/150‑1 (MQ=60)
                          GATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGG                                                                                                                        <  SRR19973065.575934/150‑1 (MQ=60)
                            TTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCT                                                                                                                      <  SRR19973065.1551480/150‑1 (MQ=60)
                             TTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTA                                                                                                                     <  SRR19973065.828730/150‑1 (MQ=60)
                               AAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACC                                                                                                                   >  SRR19973065.546974/1‑150 (MQ=60)
                                  AACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGT                                                                                                                >  SRR19973065.1872713/1‑150 (MQ=60)
                                  AACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGT                                                                                                                >  SRR19973065.1847790/1‑150 (MQ=60)
                                   ACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTG                                                                                                               <  SRR19973065.1439770/150‑1 (MQ=60)
                                   ACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTG                                                                                                               <  SRR19973065.1973040/150‑1 (MQ=60)
                                   ACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGAAGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTG                                                                                                               <  SRR19973065.3497642/150‑1 (MQ=60)
                                    CACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGC                                                                                                              <  SRR19973065.1490037/150‑1 (MQ=60)
                                    CACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGC                                                                                                              <  SRR19973065.1499433/150‑1 (MQ=60)
                                    CACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGC                                                                                                              >  SRR19973065.1665372/1‑150 (MQ=60)
                                     ACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCA                                                                                                             <  SRR19973065.823136/150‑1 (MQ=60)
                                       TGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATA                                                                                                           <  SRR19973065.986432/150‑1 (MQ=60)
                                         CTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAA                                                                                                         >  SRR19973065.3448307/1‑150 (MQ=60)
                                         CTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAA                                                                                                         >  SRR19973065.694409/1‑150 (MQ=60)
                                            CGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAA                                                                                                      <  SRR19973065.945337/150‑1 (MQ=60)
                                            CGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAA                                                                                                      <  SRR19973065.944867/150‑1 (MQ=60)
                                            CGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAA                                                                                                      >  SRR19973065.148075/1‑150 (MQ=60)
                                               GCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTT                                                                                                   <  SRR19973065.1660413/150‑1 (MQ=60)
                                                 ACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAAATGTGAAATTTTGACTTGCATGTTATAGGCTACCGGTGCATAAATAACTTTT                                                                                                 >  SRR19973065.3281838/1‑150 (MQ=60)
                                                    CACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTG                                                                                              >  SRR19973065.2571331/1‑150 (MQ=60)
                                                    CACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTG                                                                                              <  SRR19973065.474299/150‑1 (MQ=60)
                                                      CGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCG                                                                                            >  SRR19973065.3364714/1‑150 (MQ=60)
                                                      CGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCG                                                                                            >  SRR19973065.1629102/1‑150 (MQ=60)
                                                          GGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATNT                                                                                        <  SRR19973065.870555/150‑1 (MQ=60)
                                                          GGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATAT                                                                                        <  SRR19973065.1772543/150‑1 (MQ=60)
                                                          GGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATAT                                                                                        <  SRR19973065.1073055/150‑1 (MQ=60)
                                                          GGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATAT                                                                                        <  SRR19973065.1772617/150‑1 (MQ=60)
                                                            TGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCA                                                                                      >  SRR19973065.1886144/1‑150 (MQ=60)
                                                             GCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCAC                                                                                     <  SRR19973065.3316458/150‑1 (MQ=60)
                                                                  CGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAT                                                                                <  SRR19973065.939837/150‑1 (MQ=60)
                                                                  CGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAT                                                                                <  SRR19973065.3088872/150‑1 (MQ=60)
                                                                  CGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAT                                                                                <  SRR19973065.3088927/150‑1 (MQ=60)
                                                                  CGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAT                                                                                <  SRR19973065.940711/150‑1 (MQ=60)
                                                                  CGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAT                                                                                <  SRR19973065.1879208/150‑1 (MQ=60)
                                                                  CGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAAT                                                                                <  SRR19973065.185697/150‑1 (MQ=60)
                                                                    CCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGT                                                                              <  SRR19973065.950444/150‑1 (MQ=60)
                                                                    CCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGT                                                                              <  SRR19973065.2716392/150‑1 (MQ=60)
                                                                     CGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTG                                                                             <  SRR19973065.3395019/150‑1 (MQ=60)
                                                                     CGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTG                                                                             <  SRR19973065.3396028/150‑1 (MQ=60)
                                                                     CGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTG                                                                             >  SRR19973065.1194728/1‑150 (MQ=60)
                                                                      GATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGC                                                                            <  SRR19973065.1355727/150‑1 (MQ=60)
                                                                            GACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAAT                                                                      <  SRR19973065.2234610/150‑1 (MQ=60)
                                                                             gatctTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATG                                                                     <  SRR19973065.2624184/145‑1 (MQ=60)
                                                                                CCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGACATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTT                                                                  <  SRR19973065.2118473/150‑1 (MQ=60)
                                                                                CCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTT                                                                  <  SRR19973065.2600622/150‑1 (MQ=60)
                                                                                CCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTT                                                                  <  SRR19973065.2600787/150‑1 (MQ=60)
                                                                                CCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTT                                                                  <  SRR19973065.2600321/150‑1 (MQ=60)
                                                                                CCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGNT                                                                  <  SRR19973065.38263/150‑1 (MQ=60)
                                                                                  TGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAT                                                                >  SRR19973065.3531894/1‑150 (MQ=60)
                                                                                  TGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTAT                                                                <  SRR19973065.1105938/150‑1 (MQ=60)
                                                                                   GGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATT                                                               <  SRR19973065.2625837/150‑1 (MQ=60)
                                                                                   GGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATT                                                               >  SRR19973065.3214868/1‑150 (MQ=60)
                                                                                     GGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTC                                                             <  SRR19973065.3469998/150‑1 (MQ=60)
                                                                                            AAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGG                                                      <  SRR19973065.2252976/150‑1 (MQ=60)
                                                                                            AAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGG                                                      <  SRR19973065.3536018/150‑1 (MQ=60)
                                                                                                 ATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATA                                                 >  SRR19973065.2023572/1‑150 (MQ=60)
                                                                                                 ATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATA                                                 >  SRR19973065.199283/1‑150 (MQ=60)
                                                                                                  TCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATAT                                                <  SRR19973065.687359/150‑1 (MQ=60)
                                                                                                      TTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAAT                                            <  SRR19973065.597079/150‑1 (MQ=60)
                                                                                                      TTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAAT                                            <  SRR19973065.3335991/150‑1 (MQ=60)
                                                                                                      TTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAAT                                            <  SRR19973065.1061423/150‑1 (MQ=60)
                                                                                                      TTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAAT                                            <  SRR19973065.383604/150‑1 (MQ=60)
                                                                                                      TTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAAT                                            >  SRR19973065.3206348/1‑150 (MQ=60)
                                                                                                       TGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATT                                           <  SRR19973065.2205117/150‑1 (MQ=60)
                                                                                                          CAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGAC                                        <  SRR19973065.3011985/150‑1 (MQ=60)
                                                                                                          CAGCCAGCATGTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGAC                                        <  SRR19973065.3011838/150‑1 (MQ=60)
                                                                                                           AGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACG                                       >  SRR19973065.1250608/1‑150 (MQ=60)
                                                                                                           AGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATAACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGAAG                                       >  SRR19973065.1548302/1‑150 (MQ=60)
                                                                                                            GCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGT                                      <  SRR19973065.63575/150‑1 (MQ=60)
                                                                                                            GCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGT                                      >  SRR19973065.3277716/1‑150 (MQ=60)
                                                                                                            GCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGT                                      >  SRR19973065.1879605/1‑150 (MQ=60)
                                                                                                               AGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCAT                                   >  SRR19973065.2795403/1‑150 (MQ=60)
                                                                                                                 CATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCG                                 >  SRR19973065.3020999/1‑150 (MQ=60)
                                                                                                                 CATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCG                                 >  SRR19973065.2138642/1‑150 (MQ=60)
                                                                                                                 CATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCG                                 >  SRR19973065.3452281/1‑150 (MQ=60)
                                                                                                                 CATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCG                                 >  SRR19973065.3452264/1‑150 (MQ=60)
                                                                                                                  ATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGC                                >  SRR19973065.1312669/1‑150 (MQ=60)
                                                                                                                  ATTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGC                                >  SRR19973065.1313281/1‑150 (MQ=60)
                                                                                                                   TTTCTCGCCAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCC                               <  SRR19973065.2373859/150‑1 (MQ=60)
                                                                                                                           CAATCACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACG                       >  SRR19973065.932494/1‑150 (MQ=60)
                                                                                                                                ACAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAA                  <  SRR19973065.2654874/150‑1 (MQ=60)
                                                                                                                                 CAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAAT                 <  SRR19973065.2333396/150‑1 (MQ=60)
                                                                                                                                 CAATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAAATTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAAT                 >  SRR19973065.769147/1‑150 (MQ=60)
                                                                                                                                  AATAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATC                >  SRR19973065.2739595/1‑150 (MQ=60)
                                                                                                                                    TAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAC              <  SRR19973065.1535031/150‑1 (MQ=60)
                                                                                                                                    TAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAC              <  SRR19973065.1537552/150‑1 (MQ=60)
                                                                                                                                    TAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAC              <  SRR19973065.2707881/150‑1 (MQ=60)
                                                                                                                                    TAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAC              >  SRR19973065.1372015/1‑150 (MQ=60)
                                                                                                                                    TAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCAC              >  SRR19973065.2357228/1‑150 (MQ=60)
                                                                                                                                    TAGGCGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAAAAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATAGCCCACCCACGCGTAATCAC              >  SRR19973065.1371985/1‑150 (MQ=60)
                                                                                                                                        CGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAA          >  SRR19973065.3308127/1‑150 (MQ=60)
                                                                                                                                        CGTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAA          >  SRR19973065.1259955/1‑150 (MQ=60)
                                                                                                                                         GTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAG         <  SRR19973065.1764186/150‑1 (MQ=60)
                                                                                                                                         GTGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAG         <  SRR19973065.1764172/150‑1 (MQ=60)
                                                                                                                                          TGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGG        <  SRR19973065.53/150‑1 (MQ=60)
                                                                                                                                          TGGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGG        <  SRR19973065.37/150‑1 (MQ=60)
                                                                                                                                           GGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGT       <  SRR19973065.476361/150‑1 (MQ=60)
                                                                                                                                           GGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGT       <  SRR19973065.475996/150‑1 (MQ=60)
                                                                                                                                           GGCGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGT       <  SRR19973065.2844932/150‑1 (MQ=60)
                                                                                                                                             CGTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTA     >  SRR19973065.1113869/1‑150 (MQ=60)
                                                                                                                                              GTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAA    >  SRR19973065.673929/1‑150 (MQ=60)
                                                                                                                                              GTTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAA    >  SRR19973065.2735656/1‑150 (MQ=60)
                                                                                                                                               TTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAT   <  SRR19973065.743869/150‑1 (MQ=60)
                                                                                                                                               TTAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAAT   <  SRR19973065.2862549/150‑1 (MQ=60)
                                                                                                                                                TAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATT  >  SRR19973065.2480337/1‑150 (MQ=60)
                                                                                                                                                TAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATT  >  SRR19973065.2496841/1‑150 (MQ=60)
                                                                                                                                                TAATTGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATT  >  SRR19973065.3165600/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                 
CCCGGCAAACCACATTTGCCGGAAGCGATTTAAAAACACTGCTTCGTGCACCCACGACGGTGCCGTCGCCGATTGTGACGCCTGGGGCAACAAAGACATCGGTTGCCAGCCAGCATTTCTCGCCAATCACAATAGGCGTGGCGTTAATGGTGAAATGTTGACTTGCATGGTCGTGGCTACCGGTGCATAAATAACTTTTTTGCGATATCACCGAATGTGCGCCAATGGTTATTTCACCGAGGGTATATAAATTGACGTCATCGCCGACCCACGCGTAATCACCTAAGGTTAATT  >  CP009273/2121865‑2122158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑